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The map label for this gene is truA

Identifier: 46446316

GI number: 46446316

Start: 830587

End: 831375

Strand: Reverse

Name: truA

Synonym: pc0682

Alternate gene names: 46446316

Gene position: 831375-830587 (Counterclockwise)

Preceding gene: 46446317

Following gene: 46446313

Centisome position: 34.43

GC content: 36.25

Gene sequence:

>789_bases
TTGGACTACACAACCCAATCCGACTTGAGTCTCTCTAAAAGTATGCAAAATATTAAATTGAAGATTGCTTATGATGGACA
GGCGTATTTTGGCTGGCAAAAAACTCCTGCCGGCCCAAGCGTTGAAAAAACGCTTCAAAACAGTTTGGAACAAATACTGC
AACATACAATTTCTTTGCAGGCAGCAAGCCGTACAGACAAAGGTGTACATGCACGAGGGCAAATAGTCAATTTTTTAACA
ACAAAGTCTATTACAGATCTACAAAAATTTATTCTCAGTTTAAATAGTTTGCTTCCTACAGATTTACGTATCTTATCGGC
AGAAAAAATGCCTTCGACTTTTCATCCCACTTTAAATTGTGTCGCCAAGGAATATTGTTATTATATTTGTTATGATTTTG
TTCAACTTCCTGAATACCGTCCCTATTCTTGGCATTGCCCTTTCCCTCTTATGTTAGGAAAAATGACACAAGCTATTTCT
GTACTTATTGGCGAACATGACTTTTCTGCTTTTTGTAATTTTAAAAAAAATGTTAACTATACAGATTATATTCGACGTGT
CCAAGCAATTCACCTGGAAGTCCTGAATCATAAACGTCTTTGCATTCGTATTAAAGGCAATCATTTTTTATACAAAATGG
TGCGAAATATTGTGGGTACTTTAATTTATATAGGAAAGGGAAAGCTAATGGTAGAAGATATCCCTTCAATCCTTCAGTCT
CAAGATCGAAAAATGGCTGGTGTGACAGCCCCCGCACACGGACTTTTTCTTCAAACAGTCCTTTATTAA

Upstream 100 bases:

>100_bases
CTTTCTTAGGGGAAAGTGGATTGATGGCCAGTGAGAGAATGATTGGAATGGTGCTCGTTTTGATTGCAGTTCAAAGATTT
TTAGAAGGCATTTTACTTTT

Downstream 100 bases:

>100_bases
AATTTTAGGTTTATTTGCTTGTGTCTCGTTTTTTTGCCATTTAAGCTTTAGTCGAAAAAGTGGAAAGGTATTCTACAAAA
AGAACGACTCCAAATCCTAT

Product: putative tRNA-pseudouridine synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 2; tRNA-uridine isomerase I 2

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDYTTQSDLSLSKSMQNIKLKIAYDGQAYFGWQKTPAGPSVEKTLQNSLEQILQHTISLQAASRTDKGVHARGQIVNFLT
TKSITDLQKFILSLNSLLPTDLRILSAEKMPSTFHPTLNCVAKEYCYYICYDFVQLPEYRPYSWHCPFPLMLGKMTQAIS
VLIGEHDFSAFCNFKKNVNYTDYIRRVQAIHLEVLNHKRLCIRIKGNHFLYKMVRNIVGTLIYIGKGKLMVEDIPSILQS
QDRKMAGVTAPAHGLFLQTVLY

Sequences:

>Translated_262_residues
MDYTTQSDLSLSKSMQNIKLKIAYDGQAYFGWQKTPAGPSVEKTLQNSLEQILQHTISLQAASRTDKGVHARGQIVNFLT
TKSITDLQKFILSLNSLLPTDLRILSAEKMPSTFHPTLNCVAKEYCYYICYDFVQLPEYRPYSWHCPFPLMLGKMTQAIS
VLIGEHDFSAFCNFKKNVNYTDYIRRVQAIHLEVLNHKRLCIRIKGNHFLYKMVRNIVGTLIYIGKGKLMVEDIPSILQS
QDRKMAGVTAPAHGLFLQTVLY
>Mature_262_residues
MDYTTQSDLSLSKSMQNIKLKIAYDGQAYFGWQKTPAGPSVEKTLQNSLEQILQHTISLQAASRTDKGVHARGQIVNFLT
TKSITDLQKFILSLNSLLPTDLRILSAEKMPSTFHPTLNCVAKEYCYYICYDFVQLPEYRPYSWHCPFPLMLGKMTQAIS
VLIGEHDFSAFCNFKKNVNYTDYIRRVQAIHLEVLNHKRLCIRIKGNHFLYKMVRNIVGTLIYIGKGKLMVEDIPSILQS
QDRKMAGVTAPAHGLFLQTVLY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=252, Percent_Identity=31.3492063492063, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI70166645, Length=272, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI70166634, Length=272, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI70166599, Length=272, Percent_Identity=27.9411764705882, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=33.739837398374, Blast_Score=135, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321112, Length=232, Percent_Identity=26.7241379310345, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281362146, Length=266, Percent_Identity=24.812030075188, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648455, Length=266, Percent_Identity=24.812030075188, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA2_PARUW (Q6MDE3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007681.1
- ProteinModelPortal:   Q6MDE3
- SMR:   Q6MDE3
- STRING:   Q6MDE3
- GeneID:   2780742
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0682
- NMPDR:   fig|264201.1.peg.682
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   IRIIIAN
- ProtClustDB:   CLSK2762223
- BioCyc:   CPRO264201:PC0682-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29935; Mature: 29935

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: ACT_SITE 66-66

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTTQSDLSLSKSMQNIKLKIAYDGQAYFGWQKTPAGPSVEKTLQNSLEQILQHTISLQ
CCCCCCCHHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AASRTDKGVHARGQIVNFLTTKSITDLQKFILSLNSLLPTDLRILSAEKMPSTFHPTLNC
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHH
VAKEYCYYICYDFVQLPEYRPYSWHCPFPLMLGKMTQAISVLIGEHDFSAFCNFKKNVNY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCH
TDYIRRVQAIHLEVLNHKRLCIRIKGNHFLYKMVRNIVGTLIYIGKGKLMVEDIPSILQS
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHH
QDRKMAGVTAPAHGLFLQTVLY
CCCHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDYTTQSDLSLSKSMQNIKLKIAYDGQAYFGWQKTPAGPSVEKTLQNSLEQILQHTISLQ
CCCCCCCHHHHHHHHCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AASRTDKGVHARGQIVNFLTTKSITDLQKFILSLNSLLPTDLRILSAEKMPSTFHPTLNC
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHH
VAKEYCYYICYDFVQLPEYRPYSWHCPFPLMLGKMTQAISVLIGEHDFSAFCNFKKNVNY
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCH
TDYIRRVQAIHLEVLNHKRLCIRIKGNHFLYKMVRNIVGTLIYIGKGKLMVEDIPSILQS
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCEEHHHHHHHHHH
QDRKMAGVTAPAHGLFLQTVLY
CCCHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA