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The map label for this gene is pnp [H]

Identifier: 46446277

GI number: 46446277

Start: 789625

End: 791733

Strand: Reverse

Name: pnp [H]

Synonym: pc0643

Alternate gene names: 46446277

Gene position: 791733-789625 (Counterclockwise)

Preceding gene: 46446278

Following gene: 46446276

Centisome position: 32.79

GC content: 39.64

Gene sequence:

>2109_bases
ATGGAGTATTATTTAATGCAACGTGAAACTATTTCAGTTCCTGTTGGTGCACAAGAAATTATTTTTGAAACAGGTAAAAT
TGCTCGTCAAGCTGGAGGAGCTGTTGTTGTCCGATGTGGAGAAACAGTTGTCTTCACAACGGCTTGTGCCGCCCCAAATG
CTGATTCCACGACCGATTTTTTACCTCTTCGTGTTGATTATCAGGAAAAATTTTCGTCAGCAGGTAAAACACTTGGTGGA
TTTATTAAAAGAGAAGGTCGCCCGACTGAGAAAGAAGTTCTTGTTTCTCGTTTAATTGATCGTCCTATTCGCCCAATGTT
TGAAGAAGGTTATTACAATGAAGTTCAACTTCTTTCTTTTGTTTGGTCTTATGATGGAATTAACTCACCAGAACCTCTAG
CAATTTGTGGAGCTTCTGCAGCTCTAGTCATTTCCGATATTCCTCTTATTAAACCTGTTGGAGCTGTACGTATAGGGTTT
ATTGATGCTCAATTTATCGTCAACCCAACTATAGAACAACAAAAGCAATCTAAACTAGATTTGTTGATAGCAGGAACCGA
GGAAGCTGTTTTAATGATTGAAGGCTTCTGTGATTTCTTAACAGAAGATCAAGTATTGGAAGCTATTGAAATAGGCCATC
GTAGCATCAAAACGATTTGCCAAACACTTGAACAGTGGCGTGCAAAAGTGGGTAAACCAAAAAATCGTGAAACTCTTAGA
CAATTACCAAAAGAATTGTATGCAGATGTAGAATCAATTGCGAATCCCCTCTTAGAAAAAGCACTCCGAATTTGTGAAAA
GCAAAAACGGGAAGAAGCCCTAGCTGAAGTCACCAAGGCTGTCAATGATCGTTTGATGCCAGAAAACGAAGAGCCTAAAT
ACCCAGCCAAACATATCGCTTATGTCATTAAAGACGTCTCTTCTAAAATGATGCGTCAAATGATTTTAAATGAAAATGTT
CGTTCCGATGGAAGAACATCAACAGATATACGATTTATTGATATTGAACAAAGTCTTCTCCCCCGTGCTCACGGAAGCTC
TTTATTTACAAGAGGTGAAACACAAGCATTAGCTGTATGTACATTGGGCGGTGCTTCAATGGCACAACGATTTGAAGATT
TAGAAGGAGAAGGTAACAACCGTTTCTACTTACAATATTCTTTTCCTCCTTACTCAGTCGGAGAAGTAGGAAGAGTTGGA
GCTCCGGGAAGACGAGAAATCGGACATGGAAAGTTAGCGGAAAGAGCTTTAATGGCAGTCATCCCCACGAAAGAACAATT
TCCTTACACAATCCGTTTGGAATCTAACATTACAGAATCTAACGGATCCTCTTCTATGGCAACTGTTTGCGGAGGCTGCT
TAGCGCTCATGGATGCAGGTGTGGCTATTAAACGTCCAGTTGCGGGAATCGCAATGGGACTAATATTAGAAAATGAACGC
TTTATTATCCTATCAGATATTCTGGGAATTGAAGATGCGCTCGGTGATATGGATTTCAAGGTAACCGGAGATCAAAACGG
CATTACTGCTTTCCAAATGGACATCAAAGTAGAAGGCATTACCATTGAAATTATGCGGGTTGCTTTAAAACAAGCTAAAG
AAGGCCGCGTCCATATCCTAAATAAGATGCTAGCTGTATGCCCAACGTATAAGGGTGAAATGTCTCGTTATGCACCACGC
ATTGAAACGATCCAAATTAAACCAAGCAAAATTGCAGTTGTTATCGGCCCAGGTGGAAAACAAATCAGAGCAATCATCGA
ACAGACAGGTGTTCAAATTGATATTGATGATACGGGTCTTGTTAACATTGCTGCCATAGATTTAGTAAGCATTGAAAAAG
CAAAAGCCATTATTCATGGTCTGACCGCTGAAATTGAGATTGGTAGAATTTATTCTGGAAAAGCCATTTCTATTGCACCC
TTTGGTGTTTTTGTGGAAATCCTTCCCGGTAAAGAAGGCCTTTGCCATATTTCTGAATTTGATGTAAATCGTATCAACAG
CTTAGATGAATTTGTAAAACAAGGTGATATGCTCATGGTAAAAGTTCTCGATATCAACGAACGAGGACAAATTAAATTGA
GCCGCAAAGCCACTTTACAAAGTCAATAG

Upstream 100 bases:

>100_bases
TGAGAGCTAGGTTAAAAATTGTTGGTTATAAACAATATTTTTTCCTCTCAAAAATGCGTATAGAATTTTCAATTTTGATT
TATTAAAGTTTTCTTTTTTT

Downstream 100 bases:

>100_bases
CTTTTCTTTTATGGTGCTGAACAAATTCAGCACCATCTATTCCTCTTTTATAAAATTCATAAACCACGCTAATGTCTACA
AGCAAAAATATTCTTGATCA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MEYYLMQRETISVPVGAQEIIFETGKIARQAGGAVVVRCGETVVFTTACAAPNADSTTDFLPLRVDYQEKFSSAGKTLGG
FIKREGRPTEKEVLVSRLIDRPIRPMFEEGYYNEVQLLSFVWSYDGINSPEPLAICGASAALVISDIPLIKPVGAVRIGF
IDAQFIVNPTIEQQKQSKLDLLIAGTEEAVLMIEGFCDFLTEDQVLEAIEIGHRSIKTICQTLEQWRAKVGKPKNRETLR
QLPKELYADVESIANPLLEKALRICEKQKREEALAEVTKAVNDRLMPENEEPKYPAKHIAYVIKDVSSKMMRQMILNENV
RSDGRTSTDIRFIDIEQSLLPRAHGSSLFTRGETQALAVCTLGGASMAQRFEDLEGEGNNRFYLQYSFPPYSVGEVGRVG
APGRREIGHGKLAERALMAVIPTKEQFPYTIRLESNITESNGSSSMATVCGGCLALMDAGVAIKRPVAGIAMGLILENER
FIILSDILGIEDALGDMDFKVTGDQNGITAFQMDIKVEGITIEIMRVALKQAKEGRVHILNKMLAVCPTYKGEMSRYAPR
IETIQIKPSKIAVVIGPGGKQIRAIIEQTGVQIDIDDTGLVNIAAIDLVSIEKAKAIIHGLTAEIEIGRIYSGKAISIAP
FGVFVEILPGKEGLCHISEFDVNRINSLDEFVKQGDMLMVKVLDINERGQIKLSRKATLQSQ

Sequences:

>Translated_702_residues
MEYYLMQRETISVPVGAQEIIFETGKIARQAGGAVVVRCGETVVFTTACAAPNADSTTDFLPLRVDYQEKFSSAGKTLGG
FIKREGRPTEKEVLVSRLIDRPIRPMFEEGYYNEVQLLSFVWSYDGINSPEPLAICGASAALVISDIPLIKPVGAVRIGF
IDAQFIVNPTIEQQKQSKLDLLIAGTEEAVLMIEGFCDFLTEDQVLEAIEIGHRSIKTICQTLEQWRAKVGKPKNRETLR
QLPKELYADVESIANPLLEKALRICEKQKREEALAEVTKAVNDRLMPENEEPKYPAKHIAYVIKDVSSKMMRQMILNENV
RSDGRTSTDIRFIDIEQSLLPRAHGSSLFTRGETQALAVCTLGGASMAQRFEDLEGEGNNRFYLQYSFPPYSVGEVGRVG
APGRREIGHGKLAERALMAVIPTKEQFPYTIRLESNITESNGSSSMATVCGGCLALMDAGVAIKRPVAGIAMGLILENER
FIILSDILGIEDALGDMDFKVTGDQNGITAFQMDIKVEGITIEIMRVALKQAKEGRVHILNKMLAVCPTYKGEMSRYAPR
IETIQIKPSKIAVVIGPGGKQIRAIIEQTGVQIDIDDTGLVNIAAIDLVSIEKAKAIIHGLTAEIEIGRIYSGKAISIAP
FGVFVEILPGKEGLCHISEFDVNRINSLDEFVKQGDMLMVKVLDINERGQIKLSRKATLQSQ
>Mature_702_residues
MEYYLMQRETISVPVGAQEIIFETGKIARQAGGAVVVRCGETVVFTTACAAPNADSTTDFLPLRVDYQEKFSSAGKTLGG
FIKREGRPTEKEVLVSRLIDRPIRPMFEEGYYNEVQLLSFVWSYDGINSPEPLAICGASAALVISDIPLIKPVGAVRIGF
IDAQFIVNPTIEQQKQSKLDLLIAGTEEAVLMIEGFCDFLTEDQVLEAIEIGHRSIKTICQTLEQWRAKVGKPKNRETLR
QLPKELYADVESIANPLLEKALRICEKQKREEALAEVTKAVNDRLMPENEEPKYPAKHIAYVIKDVSSKMMRQMILNENV
RSDGRTSTDIRFIDIEQSLLPRAHGSSLFTRGETQALAVCTLGGASMAQRFEDLEGEGNNRFYLQYSFPPYSVGEVGRVG
APGRREIGHGKLAERALMAVIPTKEQFPYTIRLESNITESNGSSSMATVCGGCLALMDAGVAIKRPVAGIAMGLILENER
FIILSDILGIEDALGDMDFKVTGDQNGITAFQMDIKVEGITIEIMRVALKQAKEGRVHILNKMLAVCPTYKGEMSRYAPR
IETIQIKPSKIAVVIGPGGKQIRAIIEQTGVQIDIDDTGLVNIAAIDLVSIEKAKAIIHGLTAEIEIGRIYSGKAISIAP
FGVFVEILPGKEGLCHISEFDVNRINSLDEFVKQGDMLMVKVLDINERGQIKLSRKATLQSQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=723, Percent_Identity=35.6846473029046, Blast_Score=408, Evalue=1e-113,
Organism=Escherichia coli, GI145693187, Length=688, Percent_Identity=48.2558139534884, Blast_Score=641, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=730, Percent_Identity=31.2328767123288, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=35.8041958041958, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=35.8041958041958, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=35.8041958041958, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=35.5932203389831, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020588
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77329; Mature: 77329

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1 ; PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYYLMQRETISVPVGAQEIIFETGKIARQAGGAVVVRCGETVVFTTACAAPNADSTTDF
CCEEEEECCEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCCCCCE
LPLRVDYQEKFSSAGKTLGGFIKREGRPTEKEVLVSRLIDRPIRPMFEEGYYNEVQLLSF
EEEEECHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHH
VWSYDGINSPEPLAICGASAALVISDIPLIKPVGAVRIGFIDAQFIVNPTIEQQKQSKLD
HHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHHCCCEE
LLIAGTEEAVLMIEGFCDFLTEDQVLEAIEIGHRSIKTICQTLEQWRAKVGKPKNRETLR
EEEECCCCEEEEEEHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QLPKELYADVESIANPLLEKALRICEKQKREEALAEVTKAVNDRLMPENEEPKYPAKHIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YVIKDVSSKMMRQMILNENVRSDGRTSTDIRFIDIEQSLLPRAHGSSLFTRGETQALAVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCEECCCCCEEEEE
TLGGASMAQRFEDLEGEGNNRFYLQYSFPPYSVGEVGRVGAPGRREIGHGKLAERALMAV
ECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEE
IPTKEQFPYTIRLESNITESNGSSSMATVCGGCLALMDAGVAIKRPVAGIAMGLILENER
ECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHEEEEECCCC
FIILSDILGIEDALGDMDFKVTGDQNGITAFQMDIKVEGITIEIMRVALKQAKEGRVHIL
EEEEEHHHCHHHHHCCCCEEEECCCCCEEEEEEEEEEECEEHHHHHHHHHHCCCCHHHHH
NKMLAVCPTYKGEMSRYAPRIETIQIKPSKIAVVIGPGGKQIRAIIEQTGVQIDIDDTGL
HHHHHHCCCCCCHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCE
VNIAAIDLVSIEKAKAIIHGLTAEIEIGRIYSGKAISIAPFGVFVEILPGKEGLCHISEF
EEEEEEEEHHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCEEEEEEECCCCCCEEEECC
DVNRINSLDEFVKQGDMLMVKVLDINERGQIKLSRKATLQSQ
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MEYYLMQRETISVPVGAQEIIFETGKIARQAGGAVVVRCGETVVFTTACAAPNADSTTDF
CCEEEEECCEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCCCCCE
LPLRVDYQEKFSSAGKTLGGFIKREGRPTEKEVLVSRLIDRPIRPMFEEGYYNEVQLLSF
EEEEECHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHH
VWSYDGINSPEPLAICGASAALVISDIPLIKPVGAVRIGFIDAQFIVNPTIEQQKQSKLD
HHHCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHHCCCEE
LLIAGTEEAVLMIEGFCDFLTEDQVLEAIEIGHRSIKTICQTLEQWRAKVGKPKNRETLR
EEEECCCCEEEEEEHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QLPKELYADVESIANPLLEKALRICEKQKREEALAEVTKAVNDRLMPENEEPKYPAKHIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
YVIKDVSSKMMRQMILNENVRSDGRTSTDIRFIDIEQSLLPRAHGSSLFTRGETQALAVC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCEECCCCCEEEEE
TLGGASMAQRFEDLEGEGNNRFYLQYSFPPYSVGEVGRVGAPGRREIGHGKLAERALMAV
ECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEE
IPTKEQFPYTIRLESNITESNGSSSMATVCGGCLALMDAGVAIKRPVAGIAMGLILENER
ECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHEEEEECCCC
FIILSDILGIEDALGDMDFKVTGDQNGITAFQMDIKVEGITIEIMRVALKQAKEGRVHIL
EEEEEHHHCHHHHHCCCCEEEECCCCCEEEEEEEEEEECEEHHHHHHHHHHCCCCHHHHH
NKMLAVCPTYKGEMSRYAPRIETIQIKPSKIAVVIGPGGKQIRAIIEQTGVQIDIDDTGL
HHHHHHCCCCCCHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCE
VNIAAIDLVSIEKAKAIIHGLTAEIEIGRIYSGKAISIAPFGVFVEILPGKEGLCHISEF
EEEEEEEEHHHHHHHHHHHCCCEEEEEEEEECCCEEEEECCEEEEEEECCCCCCEEEECC
DVNRINSLDEFVKQGDMLMVKVLDINERGQIKLSRKATLQSQ
CHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA