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The map label for this gene is pheT [H]

Identifier: 46446003

GI number: 46446003

Start: 494708

End: 497116

Strand: Direct

Name: pheT [H]

Synonym: pc0369

Alternate gene names: 46446003

Gene position: 494708-497116 (Clockwise)

Preceding gene: 46445999

Following gene: 46446004

Centisome position: 20.49

GC content: 35.82

Gene sequence:

>2409_bases
TTGGTCATTATGCGTATACCTTTATCTTGGCTTAAAGAATATATTGATTTGAATCTTGAACCTGTTGAAATAGCTAAAAT
TTTGACAATGGCTGGTCTAGAAGTAGATGGCTATGAAGAGATAGGAAAGGGATTTTCGGGAGTTATTGTGGGAGATGTTT
TGGAAGTAGAAAAACATCCTAATGCTGATAAACTCCTTATTGCTTTGGTAAGTGATGGAAAAGAAATTCATCAAGTGGTT
TGTGGAGCTTCTAATTGTCGAAAAGGATTAAAAACAGCATTTGCACCTGTTGGAGCTTCTCTGATTGATGAAGGGAAAGA
GTTTAAAATAAAAAAAGGAAAGTTAAGAGGAATTGAATCAAACGGCATGCTTTGCTCGGCCAAAGAACTGCAGATATCAA
ATGAAGATGATGGAATTATGGAACTTTCAGAACATCTACAAGCAGGAACAGCATTAAATACGCTTTATTCGGATACCATT
TTTGAAATTTCTTTAACGCCTAATTTGGGGCATTGTGCAAGTATTGTTGGTGTAGCTCGGGAGTTATCTGCAGCGACAAG
CCATCCTCTTCGTTATCCTCATTTGGTTTTTAAAGAAGAGGGAAATCTTATTAATAACAGTCTTACACTTGATATTGAAG
CGAAAGAAGCTTGTTCAAGGTACACTTGCCGGGTGATTAAAAATGTGAAAGTAGGTGCTTCTCCAGATTGGTTAAAAGCA
AAAATCGAAAAGAGTGGTCTTCGCAGTGTAAATAATATTGTTGATGTAACTAATTATGTATTGTTAGAAATGGGGCATCC
TTTGCATGCCTTTGATTACGATCTTGTCAAAGGAAAAAAAATTGTTGTTAGAATGGCTCAAGAAGGAGAAATTTTTGAGA
CTTTGGATGGGAAAGAGCGAATTTTAAAAGATTCTCACCTAGTGATTGCTGATTCAGAAAAGCCCATAGCTATTGCTGGC
GTGATGGGAGGGCGCAATAGCGAAGTGAGCGAGAAAACAACCAATATTATTTTAGAATCTGCTTTTTTTGATCCTATTTT
TGTGCGAAAAACAAGCAAACAATTAGGATTGCAAACAGATGCTTCTAAGCGATTTGAAAGGGGCACTGACCCAAACCAAC
TGATTTCGGTTCTTAATCGCGCAGCTATGTTAATTCAAATGGTGGCTGGAGGGATAATTGAAGATTCTATTTTAGATGAG
CAAACGAAAGACTTTCTAGAATTGACAATTCGATGCAGACTATCTCGAATAAATCAGATATTGGGGACAACGCTTAGTCG
AGGAGAAGTTGAAACTATTTTTCAAAGTTTAGGATTCCATTATCAATGGGATGGACAAGATAGTTTTATGGTATGCGTGC
CTACTTACCGGACTGACGTTAAAGAAGAAATTGACCTCATTGAAGAAGTTGCTCGCATCTATGGCTACGATCACATTCCA
AGAAATGGTGGTCGTTATCAAACGTCCAAATTACCTGATGCACCGATTTATCTATTCGAAAAAGAAATTCAATCATATTT
GATTGCAGAAGGGTTACAGGAATTTATCACATGTGATTTGATTGGGCCAACTTTGGTCAATGTTGTGCAAAACCTTTCTA
TGCCTGCAGAAGCAATTGTCAAAGTTCTAAATCCTACGTCGATAGAACAATCGATTTTGAGAACATCTTTATTACCCGGA
CTTTTACAAGTTGTCAAATACAATTTGGATCATCAAAACCATCAAATCAATGGATTCGAAATTGGGCGGATCCATTTTAA
AGACGGCGATCAATATCAAGAACAATCTGTTGTTGGAATTCTTTTATCAGGCTTTTCTCAACCCTACCATTTTGAAGAGA
AAAAACGAGAGTATGATTTTTATGATTTGAAAGGAATTATAGAAAACCTTTTGAAAGAACTAGGAATTGAAAAACCGCAA
TTTAAAAATTTAGATCTTAAAACTTTTCATTCTGGGCGGCAGGCTTCTTTGTTTGTAGATGAACTTGAGGTAGGTTCTTT
TGGAGAAGTTCATCCTAGTATTCAAAGGCGTTTAGATGTTTCTCAGCGTATCTTATTTGGAGAGTTTAATTTACATGACT
TAATGCAAGTGGCAACTAAACTTGAAAAAGTCAAACCTTTAGCTATTTATCCTGGTTCTGAACGCGATTGGACTTTCACA
ATCAAGACGTCTGTACCTTTTGCTGAAATTATCAAGGCTATTCATGAGCAAAAATCTGATCTATTAGAAAATGTATTGTT
ATTAGATATTTACCGAAGTGAAAAACTGACAGTTGGTCATCAAAATATGACTCTGCGATTTATTTATCGGGATTTTTCTA
AAACAATTGCACAAGAAGTTGTAGAAAATGAGCATCAACGATTAAAAACAGCAGTTTCTCAAAAGTTTGCTAATGATCTT
AAACAATAA

Upstream 100 bases:

>100_bases
AATTTTATGATAAAATATTCTTAATATCTCCTCGAGCTTCTCAATTAAAAGAATCTATTCAGAATGAAAATCATTTAATC
CTTAAAATCTAAATCTATGT

Downstream 100 bases:

>100_bases
TTTGAGAAAATTCTCATTCAATTGGAGTGTTATATGAAGAATTCATTTTTCTTATCTTCTACTTTGTTATGTCTATTAGT
TTCTACCTCTTGTCAAACTC

Product: putative phenylalanine-tRNA ligase beta chain

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MVIMRIPLSWLKEYIDLNLEPVEIAKILTMAGLEVDGYEEIGKGFSGVIVGDVLEVEKHPNADKLLIALVSDGKEIHQVV
CGASNCRKGLKTAFAPVGASLIDEGKEFKIKKGKLRGIESNGMLCSAKELQISNEDDGIMELSEHLQAGTALNTLYSDTI
FEISLTPNLGHCASIVGVARELSAATSHPLRYPHLVFKEEGNLINNSLTLDIEAKEACSRYTCRVIKNVKVGASPDWLKA
KIEKSGLRSVNNIVDVTNYVLLEMGHPLHAFDYDLVKGKKIVVRMAQEGEIFETLDGKERILKDSHLVIADSEKPIAIAG
VMGGRNSEVSEKTTNIILESAFFDPIFVRKTSKQLGLQTDASKRFERGTDPNQLISVLNRAAMLIQMVAGGIIEDSILDE
QTKDFLELTIRCRLSRINQILGTTLSRGEVETIFQSLGFHYQWDGQDSFMVCVPTYRTDVKEEIDLIEEVARIYGYDHIP
RNGGRYQTSKLPDAPIYLFEKEIQSYLIAEGLQEFITCDLIGPTLVNVVQNLSMPAEAIVKVLNPTSIEQSILRTSLLPG
LLQVVKYNLDHQNHQINGFEIGRIHFKDGDQYQEQSVVGILLSGFSQPYHFEEKKREYDFYDLKGIIENLLKELGIEKPQ
FKNLDLKTFHSGRQASLFVDELEVGSFGEVHPSIQRRLDVSQRILFGEFNLHDLMQVATKLEKVKPLAIYPGSERDWTFT
IKTSVPFAEIIKAIHEQKSDLLENVLLLDIYRSEKLTVGHQNMTLRFIYRDFSKTIAQEVVENEHQRLKTAVSQKFANDL
KQ

Sequences:

>Translated_802_residues
MVIMRIPLSWLKEYIDLNLEPVEIAKILTMAGLEVDGYEEIGKGFSGVIVGDVLEVEKHPNADKLLIALVSDGKEIHQVV
CGASNCRKGLKTAFAPVGASLIDEGKEFKIKKGKLRGIESNGMLCSAKELQISNEDDGIMELSEHLQAGTALNTLYSDTI
FEISLTPNLGHCASIVGVARELSAATSHPLRYPHLVFKEEGNLINNSLTLDIEAKEACSRYTCRVIKNVKVGASPDWLKA
KIEKSGLRSVNNIVDVTNYVLLEMGHPLHAFDYDLVKGKKIVVRMAQEGEIFETLDGKERILKDSHLVIADSEKPIAIAG
VMGGRNSEVSEKTTNIILESAFFDPIFVRKTSKQLGLQTDASKRFERGTDPNQLISVLNRAAMLIQMVAGGIIEDSILDE
QTKDFLELTIRCRLSRINQILGTTLSRGEVETIFQSLGFHYQWDGQDSFMVCVPTYRTDVKEEIDLIEEVARIYGYDHIP
RNGGRYQTSKLPDAPIYLFEKEIQSYLIAEGLQEFITCDLIGPTLVNVVQNLSMPAEAIVKVLNPTSIEQSILRTSLLPG
LLQVVKYNLDHQNHQINGFEIGRIHFKDGDQYQEQSVVGILLSGFSQPYHFEEKKREYDFYDLKGIIENLLKELGIEKPQ
FKNLDLKTFHSGRQASLFVDELEVGSFGEVHPSIQRRLDVSQRILFGEFNLHDLMQVATKLEKVKPLAIYPGSERDWTFT
IKTSVPFAEIIKAIHEQKSDLLENVLLLDIYRSEKLTVGHQNMTLRFIYRDFSKTIAQEVVENEHQRLKTAVSQKFANDL
KQ
>Mature_802_residues
MVIMRIPLSWLKEYIDLNLEPVEIAKILTMAGLEVDGYEEIGKGFSGVIVGDVLEVEKHPNADKLLIALVSDGKEIHQVV
CGASNCRKGLKTAFAPVGASLIDEGKEFKIKKGKLRGIESNGMLCSAKELQISNEDDGIMELSEHLQAGTALNTLYSDTI
FEISLTPNLGHCASIVGVARELSAATSHPLRYPHLVFKEEGNLINNSLTLDIEAKEACSRYTCRVIKNVKVGASPDWLKA
KIEKSGLRSVNNIVDVTNYVLLEMGHPLHAFDYDLVKGKKIVVRMAQEGEIFETLDGKERILKDSHLVIADSEKPIAIAG
VMGGRNSEVSEKTTNIILESAFFDPIFVRKTSKQLGLQTDASKRFERGTDPNQLISVLNRAAMLIQMVAGGIIEDSILDE
QTKDFLELTIRCRLSRINQILGTTLSRGEVETIFQSLGFHYQWDGQDSFMVCVPTYRTDVKEEIDLIEEVARIYGYDHIP
RNGGRYQTSKLPDAPIYLFEKEIQSYLIAEGLQEFITCDLIGPTLVNVVQNLSMPAEAIVKVLNPTSIEQSILRTSLLPG
LLQVVKYNLDHQNHQINGFEIGRIHFKDGDQYQEQSVVGILLSGFSQPYHFEEKKREYDFYDLKGIIENLLKELGIEKPQ
FKNLDLKTFHSGRQASLFVDELEVGSFGEVHPSIQRRLDVSQRILFGEFNLHDLMQVATKLEKVKPLAIYPGSERDWTFT
IKTSVPFAEIIKAIHEQKSDLLENVLLLDIYRSEKLTVGHQNMTLRFIYRDFSKTIAQEVVENEHQRLKTAVSQKFANDL
KQ

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=808, Percent_Identity=35.519801980198, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 90199; Mature: 90199

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIMRIPLSWLKEYIDLNLEPVEIAKILTMAGLEVDGYEEIGKGFSGVIVGDVLEVEKHP
CEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEHHHHHHCCCC
NADKLLIALVSDGKEIHQVVCGASNCRKGLKTAFAPVGASLIDEGKEFKIKKGKLRGIES
CCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCC
NGMLCSAKELQISNEDDGIMELSEHLQAGTALNTLYSDTIFEISLTPNLGHCASIVGVAR
CCCEEEHHHCEECCCCCHHHHHHHHHHHCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHH
ELSAATSHPLRYPHLVFKEEGNLINNSLTLDIEAKEACSRYTCRVIKNVKVGASPDWLKA
HHHHHCCCCCCCCCEEEECCCCEECCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHH
KIEKSGLRSVNNIVDVTNYVLLEMGHPLHAFDYDLVKGKKIVVRMAQEGEIFETLDGKER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHCCCHHH
ILKDSHLVIADSEKPIAIAGVMGGRNSEVSEKTTNIILESAFFDPIFVRKTSKQLGLQTD
HHCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCC
ASKRFERGTDPNQLISVLNRAAMLIQMVAGGIIEDSILDEQTKDFLELTIRCRLSRINQI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTTLSRGEVETIFQSLGFHYQWDGQDSFMVCVPTYRTDVKEEIDLIEEVARIYGYDHIP
HHCCCCCCHHHHHHHHCCCEEEECCCCCEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCC
RNGGRYQTSKLPDAPIYLFEKEIQSYLIAEGLQEFITCDLIGPTLVNVVQNLSMPAEAIV
CCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
KVLNPTSIEQSILRTSLLPGLLQVVKYNLDHQNHQINGFEIGRIHFKDGDQYQEQSVVGI
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEEEECCCCCHHHHHHHHH
LLSGFSQPYHFEEKKREYDFYDLKGIIENLLKELGIEKPQFKNLDLKTFHSGRQASLFVD
HHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEE
ELEVGSFGEVHPSIQRRLDVSQRILFGEFNLHDLMQVATKLEKVKPLAIYPGSERDWTFT
CCCCCCCCCCCHHHHHHHCHHHHHEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEE
IKTSVPFAEIIKAIHEQKSDLLENVLLLDIYRSEKLTVGHQNMTLRFIYRDFSKTIAQEV
EECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCEEEHHHHHHHHHHHHHH
VENEHQRLKTAVSQKFANDLKQ
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIMRIPLSWLKEYIDLNLEPVEIAKILTMAGLEVDGYEEIGKGFSGVIVGDVLEVEKHP
CEEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEHHHHHHCCCC
NADKLLIALVSDGKEIHQVVCGASNCRKGLKTAFAPVGASLIDEGKEFKIKKGKLRGIES
CCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCCCEEEECCCCCCCCC
NGMLCSAKELQISNEDDGIMELSEHLQAGTALNTLYSDTIFEISLTPNLGHCASIVGVAR
CCCEEEHHHCEECCCCCHHHHHHHHHHHCHHHHHHHCCEEEEEEECCCCHHHHHHHHHHH
ELSAATSHPLRYPHLVFKEEGNLINNSLTLDIEAKEACSRYTCRVIKNVKVGASPDWLKA
HHHHHCCCCCCCCCEEEECCCCEECCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHH
KIEKSGLRSVNNIVDVTNYVLLEMGHPLHAFDYDLVKGKKIVVRMAQEGEIFETLDGKER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHCCCHHH
ILKDSHLVIADSEKPIAIAGVMGGRNSEVSEKTTNIILESAFFDPIFVRKTSKQLGLQTD
HHCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCC
ASKRFERGTDPNQLISVLNRAAMLIQMVAGGIIEDSILDEQTKDFLELTIRCRLSRINQI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTTLSRGEVETIFQSLGFHYQWDGQDSFMVCVPTYRTDVKEEIDLIEEVARIYGYDHIP
HHCCCCCCHHHHHHHHCCCEEEECCCCCEEEEECCHHCCHHHHHHHHHHHHHHHCCCCCC
RNGGRYQTSKLPDAPIYLFEKEIQSYLIAEGLQEFITCDLIGPTLVNVVQNLSMPAEAIV
CCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHH
KVLNPTSIEQSILRTSLLPGLLQVVKYNLDHQNHQINGFEIGRIHFKDGDQYQEQSVVGI
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEEEECCCCCHHHHHHHHH
LLSGFSQPYHFEEKKREYDFYDLKGIIENLLKELGIEKPQFKNLDLKTFHSGRQASLFVD
HHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCEEEEE
ELEVGSFGEVHPSIQRRLDVSQRILFGEFNLHDLMQVATKLEKVKPLAIYPGSERDWTFT
CCCCCCCCCCCHHHHHHHCHHHHHEEECCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEE
IKTSVPFAEIIKAIHEQKSDLLENVLLLDIYRSEKLTVGHQNMTLRFIYRDFSKTIAQEV
EECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEECCCCCEEEHHHHHHHHHHHHHH
VENEHQRLKTAVSQKFANDLKQ
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA