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The map label for this gene is mraW

Identifier: 46445945

GI number: 46445945

Start: 428439

End: 429389

Strand: Direct

Name: mraW

Synonym: pc0311

Alternate gene names: 46445945

Gene position: 428439-429389 (Clockwise)

Preceding gene: 46445942

Following gene: 46445946

Centisome position: 17.74

GC content: 37.12

Gene sequence:

>951_bases
ATGCCTATTCCTTTTTATCCCCACCGATCCGTTTTATTAGAAGAGGTGATTGAAGCATTCCAACCTGTTCAATTGAAAGT
ATTTATAGATGGAACGTTAGGAGCTGGTGGACATGCAGAAGCAATCCTTGAACATCATCCAGAAATTGAGCTCTATCTAG
GGATTGATCAAGATCCCAATGCTTTAAATATTGCAAATAAAAGACTTGAGAAATGGAAACAGAAAATTCTCTTAAAACAA
GGAAATTTTTCTCAGTTTGATATTTTTTTAAAAGAAATTGGTTTTTCTTCCATGGATGGGCTTTTGGTTGATTTAGGTGT
TTCTTCAATGCAGCTTGATCAGCCAGAAAGGGGGTTTAGTTTTTCTAAAGATGGCCCTTTAGATATGCGTATGAATCCTG
AAGGTAAGCTAACTGCCGCTGATATTGTCAATACCTGGTCAGAACATGATTTAGGAAAAATTTTTCGTGATTACGGAGAA
GAAAAAAAATGGCGTTTAGCAGCTCGTACAATCGTGCAGGCAAGGCAGGTAAAACAGATTTTGACGACAACAGATTTAGC
TAATCTTCTCAAACCTGCTTTTGCTTGGAATCCCAAAAAAGGGATTAATCCGCTTACTCTCATTTTTCAAGCTTTGCGTA
TTTGTGTGAATAGAGAATTAGATGTATTAGAGCAGTTAGTTTCGAAAACATTTGACTATTTAAAACCTGGTGGACGAGTC
GCTGTGATTAGCTTTCATAGTTTAGAAGATCGTATTGTCAAAAATGAATTGCGATTGGCGGCCAGTGATAAATGGGAAAC
GACAGGTTTAGGAAGTGGTTTATTTCGTGATAAAAAACCTGTAGCGAAACTTGTGAATCGAAAACCAATTTGCCCCCATG
AAAAAGAAATTAAAGAAAATCCGCGTAGTCGGAGTGCTAAATTTCGAATTGCTGAAAAATTGGAAGGGTAG

Upstream 100 bases:

>100_bases
GTAGATTATTAGCAAATAAATTTGAAGAAGTTAAGGGGTTTCGTTGATCTTCTAATAGAACCTTAATTATAATGTTTATG
TTAAATTTACAAGTGTAACT

Downstream 100 bases:

>100_bases
GATAACAAATGGGTCTTCTAATTAGGTTATGGATTTGTATTTTCTTTGCTGGATTAACTCTTTATAAATATATCGATAAA
TTGAATGAGTTGACTGAATT

Product: S-adenosyl-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPIPFYPHRSVLLEEVIEAFQPVQLKVFIDGTLGAGGHAEAILEHHPEIELYLGIDQDPNALNIANKRLEKWKQKILLKQ
GNFSQFDIFLKEIGFSSMDGLLVDLGVSSMQLDQPERGFSFSKDGPLDMRMNPEGKLTAADIVNTWSEHDLGKIFRDYGE
EKKWRLAARTIVQARQVKQILTTTDLANLLKPAFAWNPKKGINPLTLIFQALRICVNRELDVLEQLVSKTFDYLKPGGRV
AVISFHSLEDRIVKNELRLAASDKWETTGLGSGLFRDKKPVAKLVNRKPICPHEKEIKENPRSRSAKFRIAEKLEG

Sequences:

>Translated_316_residues
MPIPFYPHRSVLLEEVIEAFQPVQLKVFIDGTLGAGGHAEAILEHHPEIELYLGIDQDPNALNIANKRLEKWKQKILLKQ
GNFSQFDIFLKEIGFSSMDGLLVDLGVSSMQLDQPERGFSFSKDGPLDMRMNPEGKLTAADIVNTWSEHDLGKIFRDYGE
EKKWRLAARTIVQARQVKQILTTTDLANLLKPAFAWNPKKGINPLTLIFQALRICVNRELDVLEQLVSKTFDYLKPGGRV
AVISFHSLEDRIVKNELRLAASDKWETTGLGSGLFRDKKPVAKLVNRKPICPHEKEIKENPRSRSAKFRIAEKLEG
>Mature_315_residues
PIPFYPHRSVLLEEVIEAFQPVQLKVFIDGTLGAGGHAEAILEHHPEIELYLGIDQDPNALNIANKRLEKWKQKILLKQG
NFSQFDIFLKEIGFSSMDGLLVDLGVSSMQLDQPERGFSFSKDGPLDMRMNPEGKLTAADIVNTWSEHDLGKIFRDYGEE
KKWRLAARTIVQARQVKQILTTTDLANLLKPAFAWNPKKGINPLTLIFQALRICVNRELDVLEQLVSKTFDYLKPGGRVA
VISFHSLEDRIVKNELRLAASDKWETTGLGSGLFRDKKPVAKLVNRKPICPHEKEIKENPRSRSAKFRIAEKLEG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=39.3491124260355, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=43.9153439153439, Blast_Score=151, Evalue=6e-37,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=39.4904458598726, Blast_Score=202, Evalue=3e-53,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=38.4848484848485, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=38.4848484848485, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PARUW (Q6MEG4)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007310.1
- ProteinModelPortal:   Q6MEG4
- STRING:   Q6MEG4
- GeneID:   2780206
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0311
- NMPDR:   fig|264201.1.peg.311
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q6MEG4
- ProtClustDB:   CLSK2762126
- BioCyc:   CPRO264201:PC0311-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35821; Mature: 35690

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 83-83 BINDING 104-104 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIPFYPHRSVLLEEVIEAFQPVQLKVFIDGTLGAGGHAEAILEHHPEIELYLGIDQDPN
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCC
ALNIANKRLEKWKQKILLKQGNFSQFDIFLKEIGFSSMDGLLVDLGVSSMQLDQPERGFS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCC
FSKDGPLDMRMNPEGKLTAADIVNTWSEHDLGKIFRDYGEEKKWRLAARTIVQARQVKQI
CCCCCCCCEEECCCCCEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LTTTDLANLLKPAFAWNPKKGINPLTLIFQALRICVNRELDVLEQLVSKTFDYLKPGGRV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
AVISFHSLEDRIVKNELRLAASDKWETTGLGSGLFRDKKPVAKLVNRKPICPHEKEIKEN
EEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCC
PRSRSAKFRIAEKLEG
CCCCCHHHHHHHHCCC
>Mature Secondary Structure 
PIPFYPHRSVLLEEVIEAFQPVQLKVFIDGTLGAGGHAEAILEHHPEIELYLGIDQDPN
CCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCC
ALNIANKRLEKWKQKILLKQGNFSQFDIFLKEIGFSSMDGLLVDLGVSSMQLDQPERGFS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCCCCC
FSKDGPLDMRMNPEGKLTAADIVNTWSEHDLGKIFRDYGEEKKWRLAARTIVQARQVKQI
CCCCCCCCEEECCCCCEEHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LTTTDLANLLKPAFAWNPKKGINPLTLIFQALRICVNRELDVLEQLVSKTFDYLKPGGRV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCE
AVISFHSLEDRIVKNELRLAASDKWETTGLGSGLFRDKKPVAKLVNRKPICPHEKEIKEN
EEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHCC
PRSRSAKFRIAEKLEG
CCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA