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The map label for this gene is gltX

Identifier: 46445878

GI number: 46445878

Start: 342450

End: 343958

Strand: Reverse

Name: gltX

Synonym: pc0244

Alternate gene names: 46445878

Gene position: 343958-342450 (Counterclockwise)

Preceding gene: 46445879

Following gene: 46445877

Centisome position: 14.25

GC content: 38.1

Gene sequence:

>1509_bases
ATGTCAGTTCGCGTGCGCATTGCGCCTTCTCCAACAGGTGATCCCCACGTTGGAACAGCCTATATGGCTTTGTTTAATAT
GATTTTTGCACGTCATTACAATGGAACATTTATTCTTCGTATTGAAGATACAGATCGGGCTCGTAGCCGACCAGAATATG
AAGAAAATATTTATTCAGCCCTCAAATGGGCAAATATCCAATGGGATGAAGGCCCTGATATTGGGGGTGCTTATGGTCCT
TACCGACAATCAGAAAGATTTGATATTTATAAACAATATGCTGAAGAACTTTTATCTAAAGGGAAAGCCTATAAATGTTT
TTGCACTGCTGCTGAACTTGATGAAATGAGAGAATTGAGCGCTAAACAGGGTGGAAGACAAGGTTATGATAGACGTTGCA
GACACCTGACTCCTGATGAGATAATTGAGAGAGAGCAAGCCGAGCTTTCGTATGTTATTCGTTTAAAAGTCCCTTTAAAC
GGTGAATGTGTTTATGAAGATGCGATTAAAAAACGAATGACCTTTCCTTGGGCTGATATAGACGATCAAGTTCTTCTCAA
ATCAGATGGTTTTCCCACCTACCACCTGGCAAACGTTGTCGATGATTATCTTATGAAAATTTCTCATGTGATTCGAGGCG
ATGAATGGATGAGTTCTACTCCAAAGCACATCTTGCTATATGAATCCTTTGGATGGACTCCTCCAACGTTCTTGCATATG
CCTCTTTTATTAGGTAAAGATGGTAAAAAATTGTCTAAACGAAAAAATCCGACTTCCATTTTTTTTTATAGAGATAGCGG
TTATTTATCCGAAGCTTTCATCAATTTCTTAACATTGATGGGCTATAGTATGACTGGCGATCAAGAAATTTATAGCTTAG
ATGACATTATACGAGAATTTGATTACAAGCGGATTGGCGTCTCCGGAGCTATTTTTGATGTGCAGAAGTTAGACTGGGTG
AACCAACAGTACCTCATTAAAAATATTCCTGTTGAGCAACTTTGGGATAGGATCAAAGAATGGAGCTTTAACGATGAATT
TATGCAACGCCTAATGCCTCTTTGCCATTCTCGCATTAAAACTTTTGGCGATTTTATGGATCTGTTTAACTTTTTATTCA
TCAATCATCTTCATTATAATGATGCGATATTTGTCGTTAAAGATCTATCTAAAGAACAAATTTGCTACTTGATTCAATCT
TTAATTTGGCGTTTGGATGAATTAGAAAATTGGAACGGAACAGGTGTTAATCAAGCTTCCAGAGAGATTGCAGAAATATT
TGGAGTCAATCATAAGAAAATTGTCATGCCAATTCTCTTTGCTAGTTTGATGAGTAAAACACAAGGTCCTCCTCTCTTTG
ATTCCGTCAACCTTTTAGGGAAAGATCGTACACGTGCGCGACTGCTTAAAGCGATGGAATATTTAGGAGGAATTTCAAAT
AAAAAAATGGCCAGCCTCAAAAAGGCCTGGCAAGAAAAAAGCGGTCAACCGCTCATGATCAAGGATTAA

Upstream 100 bases:

>100_bases
ATTTGTAAAAAGAATTGTTAAATACAAATTCACCCACTATTTATTAAACCTTCTTATTTAGAAGGGTCACTACAGATTTT
AAAGAAAAGGCCTTAGTTTT

Downstream 100 bases:

>100_bases
AAATTCTTTTAATTAATGCTGGATGAATGAGATTTAATTCAGCATTGATTTATGCAAGTATAGGAGAGATAAAATGGATA
TGACTGCACATGCTTTTTCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSVRVRIAPSPTGDPHVGTAYMALFNMIFARHYNGTFILRIEDTDRARSRPEYEENIYSALKWANIQWDEGPDIGGAYGP
YRQSERFDIYKQYAEELLSKGKAYKCFCTAAELDEMRELSAKQGGRQGYDRRCRHLTPDEIIEREQAELSYVIRLKVPLN
GECVYEDAIKKRMTFPWADIDDQVLLKSDGFPTYHLANVVDDYLMKISHVIRGDEWMSSTPKHILLYESFGWTPPTFLHM
PLLLGKDGKKLSKRKNPTSIFFYRDSGYLSEAFINFLTLMGYSMTGDQEIYSLDDIIREFDYKRIGVSGAIFDVQKLDWV
NQQYLIKNIPVEQLWDRIKEWSFNDEFMQRLMPLCHSRIKTFGDFMDLFNFLFINHLHYNDAIFVVKDLSKEQICYLIQS
LIWRLDELENWNGTGVNQASREIAEIFGVNHKKIVMPILFASLMSKTQGPPLFDSVNLLGKDRTRARLLKAMEYLGGISN
KKMASLKKAWQEKSGQPLMIKD

Sequences:

>Translated_502_residues
MSVRVRIAPSPTGDPHVGTAYMALFNMIFARHYNGTFILRIEDTDRARSRPEYEENIYSALKWANIQWDEGPDIGGAYGP
YRQSERFDIYKQYAEELLSKGKAYKCFCTAAELDEMRELSAKQGGRQGYDRRCRHLTPDEIIEREQAELSYVIRLKVPLN
GECVYEDAIKKRMTFPWADIDDQVLLKSDGFPTYHLANVVDDYLMKISHVIRGDEWMSSTPKHILLYESFGWTPPTFLHM
PLLLGKDGKKLSKRKNPTSIFFYRDSGYLSEAFINFLTLMGYSMTGDQEIYSLDDIIREFDYKRIGVSGAIFDVQKLDWV
NQQYLIKNIPVEQLWDRIKEWSFNDEFMQRLMPLCHSRIKTFGDFMDLFNFLFINHLHYNDAIFVVKDLSKEQICYLIQS
LIWRLDELENWNGTGVNQASREIAEIFGVNHKKIVMPILFASLMSKTQGPPLFDSVNLLGKDRTRARLLKAMEYLGGISN
KKMASLKKAWQEKSGQPLMIKD
>Mature_501_residues
SVRVRIAPSPTGDPHVGTAYMALFNMIFARHYNGTFILRIEDTDRARSRPEYEENIYSALKWANIQWDEGPDIGGAYGPY
RQSERFDIYKQYAEELLSKGKAYKCFCTAAELDEMRELSAKQGGRQGYDRRCRHLTPDEIIEREQAELSYVIRLKVPLNG
ECVYEDAIKKRMTFPWADIDDQVLLKSDGFPTYHLANVVDDYLMKISHVIRGDEWMSSTPKHILLYESFGWTPPTFLHMP
LLLGKDGKKLSKRKNPTSIFFYRDSGYLSEAFINFLTLMGYSMTGDQEIYSLDDIIREFDYKRIGVSGAIFDVQKLDWVN
QQYLIKNIPVEQLWDRIKEWSFNDEFMQRLMPLCHSRIKTFGDFMDLFNFLFINHLHYNDAIFVVKDLSKEQICYLIQSL
IWRLDELENWNGTGVNQASREIAEIFGVNHKKIVMPILFASLMSKTQGPPLFDSVNLLGKDRTRARLLKAMEYLGGISNK
KMASLKKAWQEKSGQPLMIKD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=33.5341365461847, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1788743, Length=492, Percent_Identity=33.5365853658537, Blast_Score=288, Evalue=7e-79,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=29.8305084745763, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71993394, Length=492, Percent_Identity=35.5691056910569, Blast_Score=287, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=35.6435643564356, Blast_Score=281, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6324742, Length=247, Percent_Identity=27.9352226720648, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=35.030549898167, Blast_Score=294, Evalue=7e-80,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PARUW (Q6MEN1)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007243.1
- ProteinModelPortal:   Q6MEN1
- STRING:   Q6MEN1
- GeneID:   2781428
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0244
- NMPDR:   fig|264201.1.peg.244
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   YRCFTSE
- PhylomeDB:   Q6MEN1
- ProtClustDB:   PRK01406
- BioCyc:   CPRO264201:PC0244-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 58421; Mature: 58290

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRVRIAPSPTGDPHVGTAYMALFNMIFARHYNGTFILRIEDTDRARSRPEYEENIYSA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHH
LKWANIQWDEGPDIGGAYGPYRQSERFDIYKQYAEELLSKGKAYKCFCTAAELDEMRELS
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
AKQGGRQGYDRRCRHLTPDEIIEREQAELSYVIRLKVPLNGECVYEDAIKKRMTFPWADI
HHCCCCCCHHHHHCCCCHHHHHHHHHCCEEEEEEEEECCCCCEEEHHHHHHHCCCCCCCC
DDQVLLKSDGFPTYHLANVVDDYLMKISHVIRGDEWMSSTPKHILLYESFGWTPPTFLHM
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCHHHHH
PLLLGKDGKKLSKRKNPTSIFFYRDSGYLSEAFINFLTLMGYSMTGDQEIYSLDDIIREF
HHHCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DYKRIGVSGAIFDVQKLDWVNQQYLIKNIPVEQLWDRIKEWSFNDEFMQRLMPLCHSRIK
CCHHCCCCCCEEHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TFGDFMDLFNFLFINHLHYNDAIFVVKDLSKEQICYLIQSLIWRLDELENWNGTGVNQAS
HHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
REIAEIFGVNHKKIVMPILFASLMSKTQGPPLFDSVNLLGKDRTRARLLKAMEYLGGISN
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
KKMASLKKAWQEKSGQPLMIKD
HHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
SVRVRIAPSPTGDPHVGTAYMALFNMIFARHYNGTFILRIEDTDRARSRPEYEENIYSA
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHH
LKWANIQWDEGPDIGGAYGPYRQSERFDIYKQYAEELLSKGKAYKCFCTAAELDEMRELS
HHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
AKQGGRQGYDRRCRHLTPDEIIEREQAELSYVIRLKVPLNGECVYEDAIKKRMTFPWADI
HHCCCCCCHHHHHCCCCHHHHHHHHHCCEEEEEEEEECCCCCEEEHHHHHHHCCCCCCCC
DDQVLLKSDGFPTYHLANVVDDYLMKISHVIRGDEWMSSTPKHILLYESFGWTPPTFLHM
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCCCHHHHH
PLLLGKDGKKLSKRKNPTSIFFYRDSGYLSEAFINFLTLMGYSMTGDQEIYSLDDIIREF
HHHCCCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DYKRIGVSGAIFDVQKLDWVNQQYLIKNIPVEQLWDRIKEWSFNDEFMQRLMPLCHSRIK
CCHHCCCCCCEEHHHHHHHCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TFGDFMDLFNFLFINHLHYNDAIFVVKDLSKEQICYLIQSLIWRLDELENWNGTGVNQAS
HHHHHHHHHHHHHHHHEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
REIAEIFGVNHKKIVMPILFASLMSKTQGPPLFDSVNLLGKDRTRARLLKAMEYLGGISN
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCC
KKMASLKKAWQEKSGQPLMIKD
HHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA