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The map label for this gene is tsp [H]

Identifier: 46445848

GI number: 46445848

Start: 308026

End: 310041

Strand: Direct

Name: tsp [H]

Synonym: pc0214

Alternate gene names: 46445848

Gene position: 308026-310041 (Clockwise)

Preceding gene: 46445847

Following gene: 46445851

Centisome position: 12.76

GC content: 34.18

Gene sequence:

>2016_bases
ATGAATAAAAATTGGTTGAAGATATGGTTTGTAACTTTGATGACTATCTTAGTTTCTCTAGAAGCTCGAGAGCAAGATTT
ATTAAAATCTAGTGATATCGATCGAATCATGAGCCAGATTTTAAGCCAGCATGTCGATAAGCGAACCATGACAAGTAAAA
TTTTGAATAGCTCTATAGCGATTTTTATTAATCAATTCGATCCTGAACGTATTTACCTACTAGAGAGCGAAATAGCTCCT
TATGCGCATTTAACTCAAAAGCAACTTGATGAATTAGTGGAACAATATAAACAAAACAATTTTGAATTTTTCGAGCAAAT
AAACAAAGTTTTTCAAAATGCTATTGAGCGTTCAAAAAAACTTCGTCATGAAATAGAAATTAAAGATAAAGAAGCCTTGT
TTCAAACAACAAGGCCCTCGGTTCAAAGAATTGAAGAAAGATCATTTGCTAAGGATGAAAAAGAATTAAAAAAACGGCTG
CTAGAAAATTTAGAAAATTTTATTAGCCTATATAAAAGTCGTCATGGAGAGCTTTCTACAAGTTCTAAAAAAGAATTTGT
TTTGAAAACTTATGAAAATCATTTAACAGAATTCGAAAATCAATATCTTTATCAAGATGATCAGGGGAAACCTTTATCTT
ACGCACAACAAGATAATTTATTGACGATTCATATTTTAAAAGCCCTTGCAAGTAGTTTAGATTCTCACACGACTTTTTAT
CAAACTCACGAAGCTTATGACATGAAAGTTCGATTGCAAAAAGAATTTCAAGGAATTGGACTAGTTTTACGCGATCAAGG
AGATAAAATTATTGTATCTCGAATGTTAGAGGGTGGGCCAGCCGCAAAAAGTGGAGTTATCCAGGCAGGAGATATTTTGC
TAGGGGTAGATGGAATTCCAACAGAAAAACTTTCTTTTGATAAAGTCATGGAAATGTTGCATGAATCTAAACAAGCACAA
GTAGAGCTGTCTTTCAAACGAAAAAAAAAAGATGAGATAGAAGATAAAATTTTTAAAGTAGAACTTCAAAAAGAAGAAAT
TGTTTTAAACAATAATCGAGTTGATGTTAGCGAAGAAACTTTTGGCAATGGAATTATTGGCAAAATTACTCTTCATTCTT
TTTATCAAGGAAATGGAATTTCCAGTGAAAAAGATGTTCGAGACGCGATTATAGAATTAGAAAAAAAAGGTAATTTAAGA
GGGTTGATTTTAGATCTTCGAGAAAATAGTGGCGGATTTCTTTCGCAAGCTGTTAAAGTAGCAGGCCTTTTTATTACTAA
CGGGGTCATTGTTATTTCTAAGTACTCCACTGGTGAAGAGCGCTTTTATCGCGATGTGGATGGAAAAATAGCTTATGATG
GACCTCTCGTTGTTTTGATTTCTAAAGCTACGGCTTCTGCAGCAGAAATTGTGGCTCAGGCGTTACAAGATTATGGTGTC
GCAATTGTGGTTGGAGATGAGCATACCTATGGGAAAGGGACCATTCAAACCCAAACCGTAACCGATGATCAAAGTACGTC
TTATTTTAAAGTAACAGTTGGAAAATATTATACAGTTTCTGGAAAGACTCCTCAAAAAAATGGGGTAAAAGCCGATGTCA
TAGCTCCTGGACCTTGGAGTCTTAAAAATATTGGGGAAGTAGATTCTAGCTCTCTCACTTCCGACTTCATTTCTGATGCT
TATGAAGACAATTTATCTGACATCGCTCCGGATGTTAAATCGTGGTATTTGAAATATTATGTTCCAACCATTCAGAAACG
TACTGATCAATGGAAAGATTTAATTCCCACACTCCGAAAAAATAGCGAATATCGACTAGCACATAATAAAAACTACCAAT
TATTTCTAAAAGGAAAAGCGCTCGACCTAGAAAATGAAGAGCAGGATGAAGAGTCGGAATGGTTATCAACTGGGAAAAAA
GCTAAGAATTTTGGGGTTGACGATTTGCAAATGCAAGAAGCTGTCAACGTAATTAAAGATATGATTGTTCTCTATTCTAT
TGAAAAGAAAAAATAG

Upstream 100 bases:

>100_bases
CATTTGAATATACTGGAAATTTTGAATTGAGAGTGGATAAGATTAAAAGTCAATGATCATTTCCTTTTGGTAAAAATAGT
TGTTTCAAGGAGCGTTATTT

Downstream 100 bases:

>100_bases
AGGTGTCAAGCCTCCTGATACAGATTCAGGAGGCTTGATAAAAGGTAAGAAAAGAAACTTGTATAAAAACGTTGCACAAA
AAACAACCATTCAATAAAGT

Product: putative carboxy-terminal (= tail-specific) proteinase

Products: NA

Alternate protein names: C-terminal-processing peptidase; PRC protein; Protease Re [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MNKNWLKIWFVTLMTILVSLEAREQDLLKSSDIDRIMSQILSQHVDKRTMTSKILNSSIAIFINQFDPERIYLLESEIAP
YAHLTQKQLDELVEQYKQNNFEFFEQINKVFQNAIERSKKLRHEIEIKDKEALFQTTRPSVQRIEERSFAKDEKELKKRL
LENLENFISLYKSRHGELSTSSKKEFVLKTYENHLTEFENQYLYQDDQGKPLSYAQQDNLLTIHILKALASSLDSHTTFY
QTHEAYDMKVRLQKEFQGIGLVLRDQGDKIIVSRMLEGGPAAKSGVIQAGDILLGVDGIPTEKLSFDKVMEMLHESKQAQ
VELSFKRKKKDEIEDKIFKVELQKEEIVLNNNRVDVSEETFGNGIIGKITLHSFYQGNGISSEKDVRDAIIELEKKGNLR
GLILDLRENSGGFLSQAVKVAGLFITNGVIVISKYSTGEERFYRDVDGKIAYDGPLVVLISKATASAAEIVAQALQDYGV
AIVVGDEHTYGKGTIQTQTVTDDQSTSYFKVTVGKYYTVSGKTPQKNGVKADVIAPGPWSLKNIGEVDSSSLTSDFISDA
YEDNLSDIAPDVKSWYLKYYVPTIQKRTDQWKDLIPTLRKNSEYRLAHNKNYQLFLKGKALDLENEEQDEESEWLSTGKK
AKNFGVDDLQMQEAVNVIKDMIVLYSIEKKK

Sequences:

>Translated_671_residues
MNKNWLKIWFVTLMTILVSLEAREQDLLKSSDIDRIMSQILSQHVDKRTMTSKILNSSIAIFINQFDPERIYLLESEIAP
YAHLTQKQLDELVEQYKQNNFEFFEQINKVFQNAIERSKKLRHEIEIKDKEALFQTTRPSVQRIEERSFAKDEKELKKRL
LENLENFISLYKSRHGELSTSSKKEFVLKTYENHLTEFENQYLYQDDQGKPLSYAQQDNLLTIHILKALASSLDSHTTFY
QTHEAYDMKVRLQKEFQGIGLVLRDQGDKIIVSRMLEGGPAAKSGVIQAGDILLGVDGIPTEKLSFDKVMEMLHESKQAQ
VELSFKRKKKDEIEDKIFKVELQKEEIVLNNNRVDVSEETFGNGIIGKITLHSFYQGNGISSEKDVRDAIIELEKKGNLR
GLILDLRENSGGFLSQAVKVAGLFITNGVIVISKYSTGEERFYRDVDGKIAYDGPLVVLISKATASAAEIVAQALQDYGV
AIVVGDEHTYGKGTIQTQTVTDDQSTSYFKVTVGKYYTVSGKTPQKNGVKADVIAPGPWSLKNIGEVDSSSLTSDFISDA
YEDNLSDIAPDVKSWYLKYYVPTIQKRTDQWKDLIPTLRKNSEYRLAHNKNYQLFLKGKALDLENEEQDEESEWLSTGKK
AKNFGVDDLQMQEAVNVIKDMIVLYSIEKKK
>Mature_671_residues
MNKNWLKIWFVTLMTILVSLEAREQDLLKSSDIDRIMSQILSQHVDKRTMTSKILNSSIAIFINQFDPERIYLLESEIAP
YAHLTQKQLDELVEQYKQNNFEFFEQINKVFQNAIERSKKLRHEIEIKDKEALFQTTRPSVQRIEERSFAKDEKELKKRL
LENLENFISLYKSRHGELSTSSKKEFVLKTYENHLTEFENQYLYQDDQGKPLSYAQQDNLLTIHILKALASSLDSHTTFY
QTHEAYDMKVRLQKEFQGIGLVLRDQGDKIIVSRMLEGGPAAKSGVIQAGDILLGVDGIPTEKLSFDKVMEMLHESKQAQ
VELSFKRKKKDEIEDKIFKVELQKEEIVLNNNRVDVSEETFGNGIIGKITLHSFYQGNGISSEKDVRDAIIELEKKGNLR
GLILDLRENSGGFLSQAVKVAGLFITNGVIVISKYSTGEERFYRDVDGKIAYDGPLVVLISKATASAAEIVAQALQDYGV
AIVVGDEHTYGKGTIQTQTVTDDQSTSYFKVTVGKYYTVSGKTPQKNGVKADVIAPGPWSLKNIGEVDSSSLTSDFISDA
YEDNLSDIAPDVKSWYLKYYVPTIQKRTDQWKDLIPTLRKNSEYRLAHNKNYQLFLKGKALDLENEEQDEESEWLSTGKK
AKNFGVDDLQMQEAVNVIKDMIVLYSIEKKK

Specific function: Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3 (PBP3). May be involved in protection of the bacterium from thermal and osmotic stresses [H]

COG id: COG0793

COG function: function code M; Periplasmic protease

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1788134, Length=326, Percent_Identity=35.5828220858896, Blast_Score=175, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR005151
- InterPro:   IPR004447
- InterPro:   IPR020992 [H]

Pfam domain/function: PF11818 DUF3340; PF00595 PDZ; PF03572 Peptidase_S41 [H]

EC number: =3.4.21.102 [H]

Molecular weight: Translated: 76614; Mature: 76614

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNWLKIWFVTLMTILVSLEAREQDLLKSSDIDRIMSQILSQHVDKRTMTSKILNSSIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFINQFDPERIYLLESEIAPYAHLTQKQLDELVEQYKQNNFEFFEQINKVFQNAIERSKK
EEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LRHEIEIKDKEALFQTTRPSVQRIEERSFAKDEKELKKRLLENLENFISLYKSRHGELST
HHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SSKKEFVLKTYENHLTEFENQYLYQDDQGKPLSYAQQDNLLTIHILKALASSLDSHTTFY
CCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEE
QTHEAYDMKVRLQKEFQGIGLVLRDQGDKIIVSRMLEGGPAAKSGVIQAGDILLGVDGIP
ECCCCHHHEEEEHHHHCCCCEEEECCCCEEHHHHHHCCCCCCCCCCEEECCEEEECCCCC
TEKLSFDKVMEMLHESKQAQVELSFKRKKKDEIEDKIFKVELQKEEIVLNNNRVDVSEET
CCCCCHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHEEEEEECCEEEEECCEECCCHHH
FGNGIIGKITLHSFYQGNGISSEKDVRDAIIELEKKGNLRGLILDLRENSGGFLSQAVKV
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHH
AGLFITNGVIVISKYSTGEERFYRDVDGKIAYDGPLVVLISKATASAAEIVAQALQDYGV
HHHEEECCEEEEEECCCCHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHCCE
AIVVGDEHTYGKGTIQTQTVTDDQSTSYFKVTVGKYYTVSGKTPQKNGVKADVIAPGPWS
EEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECEEEEECCCCCCCCCCEEEEECCCCCC
LKNIGEVDSSSLTSDFISDAYEDNLSDIAPDVKSWYLKYYVPTIQKRTDQWKDLIPTLRK
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NSEYRLAHNKNYQLFLKGKALDLENEEQDEESEWLSTGKKAKNFGVDDLQMQEAVNVIKD
CCCEEEEECCCEEEEEECCEECCCCCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHH
MIVLYSIEKKK
HHHHHEECCCC
>Mature Secondary Structure
MNKNWLKIWFVTLMTILVSLEAREQDLLKSSDIDRIMSQILSQHVDKRTMTSKILNSSIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IFINQFDPERIYLLESEIAPYAHLTQKQLDELVEQYKQNNFEFFEQINKVFQNAIERSKK
EEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LRHEIEIKDKEALFQTTRPSVQRIEERSFAKDEKELKKRLLENLENFISLYKSRHGELST
HHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SSKKEFVLKTYENHLTEFENQYLYQDDQGKPLSYAQQDNLLTIHILKALASSLDSHTTFY
CCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEE
QTHEAYDMKVRLQKEFQGIGLVLRDQGDKIIVSRMLEGGPAAKSGVIQAGDILLGVDGIP
ECCCCHHHEEEEHHHHCCCCEEEECCCCEEHHHHHHCCCCCCCCCCEEECCEEEECCCCC
TEKLSFDKVMEMLHESKQAQVELSFKRKKKDEIEDKIFKVELQKEEIVLNNNRVDVSEET
CCCCCHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHEEEEEECCEEEEECCEECCCHHH
FGNGIIGKITLHSFYQGNGISSEKDVRDAIIELEKKGNLRGLILDLRENSGGFLSQAVKV
CCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHH
AGLFITNGVIVISKYSTGEERFYRDVDGKIAYDGPLVVLISKATASAAEIVAQALQDYGV
HHHEEECCEEEEEECCCCHHHHHHCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHCCE
AIVVGDEHTYGKGTIQTQTVTDDQSTSYFKVTVGKYYTVSGKTPQKNGVKADVIAPGPWS
EEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECEEEEECCCCCCCCCCEEEEECCCCCC
LKNIGEVDSSSLTSDFISDAYEDNLSDIAPDVKSWYLKYYVPTIQKRTDQWKDLIPTLRK
CCCCCCCCCHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NSEYRLAHNKNYQLFLKGKALDLENEEQDEESEWLSTGKKAKNFGVDDLQMQEAVNVIKD
CCCEEEEECCCEEEEEECCEECCCCCCCCHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHH
MIVLYSIEKKK
HHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1856173; 1729701; 9097040; 9278503; 1447154; 10049386; 7499412; 8576225 [H]