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The map label for this gene is pcnB [H]

Identifier: 46445774

GI number: 46445774

Start: 214667

End: 215905

Strand: Direct

Name: pcnB [H]

Synonym: pc0140

Alternate gene names: 46445774

Gene position: 214667-215905 (Clockwise)

Preceding gene: 46445772

Following gene: 46445775

Centisome position: 8.89

GC content: 36.24

Gene sequence:

>1239_bases
ATGGATGTTTTTAAACAAGCTAAAATTATCGTAACTAAATTGGTGAAAGCAGGACATACAGCTTATTTTGCTGGTGGATG
GGTAAGAGACTATGTAATGGGACATCCATCAGAAGATATTGATATTGCCACAGATGCGACTCCTGCACAAATTATGGATA
TTTTCCCTAATACGTTGTTGGTAGGGCTTGCTTTTGGGGTGGTCATTGTTGTTTGGGAAGGCCATCAATTTGAAGTGGCT
ACGTTTCGCAAAGATTTAGATTATGTGGATGGGAGACACCCATCAGGTATTGTTCTTTCAACAGCACGAGAAGACGCTTT
GCGGCGTGATTTTACAATTAACGGAATGTTTTACGATCCTTTGAAAGAAGTTATTTATGATTTTGTGAATGGACAAGAGG
ATATTCGAAGGCATCTTATTCGAGCGATTGGAAATCCTCATGAGAGATTTCAAGAGGATCGGTTAAGAATGCTACGAGCA
TTTCGCTTTTCTGCGCGTTTTGGATTCGCCATCGAACTTGAAACTCAGCAAGCTATTTTTGAAAATGCCGAGCAGCTTTT
CCCAGCTGTAGCGATCGAAAGAATTTGGCAAGAATTTAAAAAGATGGCTATGTATCCATGTTTTGATCAGGCTATTGTAG
AGATGCATAGATTGACTTTATTAGATGTCATTTTTCCAGAGTTAAAAGGATTACCAATTAATGAATTGAGGCATTTAGTA
GAAGCCTATAAACGATTCCCTAAAAATTGTGCGACTATTTTTTATTTGATAGTATTGATGCCTAGGCTGAATCGAGAAGA
AAAGATAGAAGTGGCTAAACGTCTTAAGGTCACAAATCGTGATCTTAAGCTTCTTCACTATTTTGAAGATTTAAACCAGC
TAATTAAAAAAGAAAAAGAAAACGGATTGGAAGATCTTTATCCTTGGGCTCATGCTTTAGCTAATCCTGATTTCTCTATT
GTAGTAGAAGTGATTGCAGCAACTTTTCCCTTAAATAAAGGGAATGAATTGTTGATTCAACTTAAAAACCGTTTTGAAAA
GTTAAAATCCCACATTGAACGAATTCAACAAAAAACACCTTTGATTTCTTCTGCACTTTTACAAGCTAATGGAATTTCGG
CAGGTAAGCAGATGGGTATACTTTTAACAGAAGCTGAGAAATTGGCTATTAATAATGATTATCAAGATTCGCAAACGGTA
TTGAACGCTTTAAAACAAACAATGTTATGGAAAATATGA

Upstream 100 bases:

>100_bases
ATAATTAAATGAATGGAATTTGCAGATTTATTTCTGATATTTGATATGATTATACTTTTATAATCATTAAATTTACACGT
TTAAAAGATATAGATATCTC

Downstream 100 bases:

>100_bases
CACCTCATTTTATCACTAGTTTACAACATCCTTTTGTCAAACATTTGGTTAAATTAAGGGAAAATAGTCGTTATCGTTTA
GAACATAAAGCATTAATTTT

Product: putative poly A polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MDVFKQAKIIVTKLVKAGHTAYFAGGWVRDYVMGHPSEDIDIATDATPAQIMDIFPNTLLVGLAFGVVIVVWEGHQFEVA
TFRKDLDYVDGRHPSGIVLSTAREDALRRDFTINGMFYDPLKEVIYDFVNGQEDIRRHLIRAIGNPHERFQEDRLRMLRA
FRFSARFGFAIELETQQAIFENAEQLFPAVAIERIWQEFKKMAMYPCFDQAIVEMHRLTLLDVIFPELKGLPINELRHLV
EAYKRFPKNCATIFYLIVLMPRLNREEKIEVAKRLKVTNRDLKLLHYFEDLNQLIKKEKENGLEDLYPWAHALANPDFSI
VVEVIAATFPLNKGNELLIQLKNRFEKLKSHIERIQQKTPLISSALLQANGISAGKQMGILLTEAEKLAINNDYQDSQTV
LNALKQTMLWKI

Sequences:

>Translated_412_residues
MDVFKQAKIIVTKLVKAGHTAYFAGGWVRDYVMGHPSEDIDIATDATPAQIMDIFPNTLLVGLAFGVVIVVWEGHQFEVA
TFRKDLDYVDGRHPSGIVLSTAREDALRRDFTINGMFYDPLKEVIYDFVNGQEDIRRHLIRAIGNPHERFQEDRLRMLRA
FRFSARFGFAIELETQQAIFENAEQLFPAVAIERIWQEFKKMAMYPCFDQAIVEMHRLTLLDVIFPELKGLPINELRHLV
EAYKRFPKNCATIFYLIVLMPRLNREEKIEVAKRLKVTNRDLKLLHYFEDLNQLIKKEKENGLEDLYPWAHALANPDFSI
VVEVIAATFPLNKGNELLIQLKNRFEKLKSHIERIQQKTPLISSALLQANGISAGKQMGILLTEAEKLAINNDYQDSQTV
LNALKQTMLWKI
>Mature_412_residues
MDVFKQAKIIVTKLVKAGHTAYFAGGWVRDYVMGHPSEDIDIATDATPAQIMDIFPNTLLVGLAFGVVIVVWEGHQFEVA
TFRKDLDYVDGRHPSGIVLSTAREDALRRDFTINGMFYDPLKEVIYDFVNGQEDIRRHLIRAIGNPHERFQEDRLRMLRA
FRFSARFGFAIELETQQAIFENAEQLFPAVAIERIWQEFKKMAMYPCFDQAIVEMHRLTLLDVIFPELKGLPINELRHLV
EAYKRFPKNCATIFYLIVLMPRLNREEKIEVAKRLKVTNRDLKLLHYFEDLNQLIKKEKENGLEDLYPWAHALANPDFSI
VVEVIAATFPLNKGNELLIQLKNRFEKLKSHIERIQQKTPLISSALLQANGISAGKQMGILLTEAEKLAINNDYQDSQTV
LNALKQTMLWKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=418, Percent_Identity=30.3827751196172, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI87081691, Length=189, Percent_Identity=38.6243386243386, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=32.579185520362, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71995920, Length=380, Percent_Identity=28.4210526315789, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321016, Length=236, Percent_Identity=29.6610169491525, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281360112, Length=304, Percent_Identity=33.2236842105263, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357337, Length=304, Percent_Identity=33.2236842105263, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 47545; Mature: 47545

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFKQAKIIVTKLVKAGHTAYFAGGWVRDYVMGHPSEDIDIATDATPAQIMDIFPNTLL
CCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VGLAFGVVIVVWEGHQFEVATFRKDLDYVDGRHPSGIVLSTAREDALRRDFTINGMFYDP
HHHHHHHEEEEECCCEEEHHHHHHHHHHCCCCCCCCEEEECHHHHHHHCCCEECCEEHHH
LKEVIYDFVNGQEDIRRHLIRAIGNPHERFQEDRLRMLRAFRFSARFGFAIELETQQAIF
HHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
ENAEQLFPAVAIERIWQEFKKMAMYPCFDQAIVEMHRLTLLDVIFPELKGLPINELRHLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EAYKRFPKNCATIFYLIVLMPRLNREEKIEVAKRLKVTNRDLKLLHYFEDLNQLIKKEKE
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NGLEDLYPWAHALANPDFSIVVEVIAATFPLNKGNELLIQLKNRFEKLKSHIERIQQKTP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LISSALLQANGISAGKQMGILLTEAEKLAINNDYQDSQTVLNALKQTMLWKI
HHHHHHHHHCCCCCCHHHCEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVFKQAKIIVTKLVKAGHTAYFAGGWVRDYVMGHPSEDIDIATDATPAQIMDIFPNTLL
CCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VGLAFGVVIVVWEGHQFEVATFRKDLDYVDGRHPSGIVLSTAREDALRRDFTINGMFYDP
HHHHHHHEEEEECCCEEEHHHHHHHHHHCCCCCCCCEEEECHHHHHHHCCCEECCEEHHH
LKEVIYDFVNGQEDIRRHLIRAIGNPHERFQEDRLRMLRAFRFSARFGFAIELETQQAIF
HHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHH
ENAEQLFPAVAIERIWQEFKKMAMYPCFDQAIVEMHRLTLLDVIFPELKGLPINELRHLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EAYKRFPKNCATIFYLIVLMPRLNREEKIEVAKRLKVTNRDLKLLHYFEDLNQLIKKEKE
HHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NGLEDLYPWAHALANPDFSIVVEVIAATFPLNKGNELLIQLKNRFEKLKSHIERIQQKTP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
LISSALLQANGISAGKQMGILLTEAEKLAINNDYQDSQTVLNALKQTMLWKI
HHHHHHHHHCCCCCCHHHCEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA