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The map label for this gene is cocE [H]

Identifier: 46445767

GI number: 46445767

Start: 202912

End: 204597

Strand: Direct

Name: cocE [H]

Synonym: pc0133

Alternate gene names: 46445767

Gene position: 202912-204597 (Clockwise)

Preceding gene: 46445766

Following gene: 46445768

Centisome position: 8.4

GC content: 38.91

Gene sequence:

>1686_bases
ATGATGATTCGTTTATATACATGTATTTTTGTATTTCTTTTAAATATTTTAGGGTCTGAGGAGCTTAAACCAGATTTAAC
AATCATGGTTCCCATGAGAGATGGAGTATCTCTTCCCACAGATCTTTATTTGCCAACTCCAGAAGCTCGAAATCTTCCTT
GCATTTTGATTAGAAGCCCTGCTGGTCGCGATTCGACATGGAAAAGTTTCGCCTCAATGGCAAAAGCAGGATATGTGATT
GCAATTCAAGATACGCGTAGTGTATTAGATCTTGAAGGAAAAACATTTCCTTTTTTGTCAGATGGATGGGGAAAATTGCA
AGATGGATATGATGCGGTAGAATGGCTGGCTAAAAGTCCCTATACGAATGGAAAAATTGGAACATGGGGTTCTTCAGCTG
TAGGAATCACGCAACTACTCCTGGCTCCCACTCAACCTCCGCATTTGGTTTGCCAATACATTATTGTAGCTGCAGCGAGT
TTATATCATCATGGACTTTTTCCTGGAGGACTTTTGCTCAAGCATCAAGCTGAAAGTTGGCTTGGTTATTACGCTCGTGA
CACTGGTGTTCTTAATTACGTTTCTCAACGGCCTTTCTATAACGAGTTTTGGAAACAATTAAATACCTCTGAAATGGCCC
ATCGAGTGAAAATCCCCGGAATGTTAGTCGCAGGTTGGTATGATACTTTCTTACAAGGAACTTTAGATGCATTTGAAGCA
AGACAACGAGAAGGTGAAGAAGGGGCTAAAGGAAAACAAAAATTAATTATTGGCCCTTGGACACACTTTTGGCCTTTATC
TAATCATTTCGGTGATTTTAAAATCCCTGTTGCAGGGGAAAATCCTCCTATGGATATTTCTCCAAAACGTTGGTTTGATC
ATTATTTAAAGGGAGAAGTAAATGAAGTTGAATCTCTACCGCCCGTCCTTTATTATGTGATGGGGCCATTTGATGGATCA
ACTTCAAGCGGAAATATTTGGCGTACATCTGAGGTATGGCCTGTACCCACTGTGGCTACAGCTTTTTATTTAACTTCTCA
ACACACACTTCAACAAACTCCTCCCTCTCTAGGCCTTTTAGCTTATACGTATGACCCAACTAACCCTATTCCCACTAGAG
GTGGGCGTAATTTATTTTTAGAATCAGGTCCTGTAGATCAACAAAATATTGAAAAAAGAAAGGATATCCTTGTATTTACA
ACACATCCTCTGAAGGAAGATCTAGAAATAACGGGAAAGTTATCGGCAAAATTGTTTGTTACCTCAGATCAACAAGACTC
AGACATTGTTGTTCATCTTACAGACGTTTATCCGGATGGTAAAAGTGTTTTGATTTCAGAAGGGGGTATACGTTTAGGAG
TATTTTGTTATCAAAATGATTCAAAAATTGACTTAAAGCCAGACCAACCAGTTGAAGTAGATCTCGACTTATGGTCCACA
AGTTTAGTTTTTGCGAAAGGACATTCAATTCGAATTTCTATTAGTAGTTCTAACTATCCGCGCTACGAAAAAAATCTTAA
TGTGGGATTAATTGGCAGCCAGAGAGGAGAATTTAAATCTGCACACAATACCATTTATATGGGAGAAAAATACCCTTCAC
AACTTATTTTGCCTGTTGTTCGTAAGGGAGAAACTTGGTTGACAAGAGAGCAACCATCTGTCAAGCCTATGGAAAATTCC
TTATGA

Upstream 100 bases:

>100_bases
TATAAAATCTCTTGTATAACTTAATTTGGATAAAGTTGATGTTAAACAAGAGATTTTGTGTATTTATAGATTTTAAATTT
ATCTACAAAACAAGCTTGTG

Downstream 100 bases:

>100_bases
AAGTCGTAATTTTAGCAGCAGGAAAAGGAAGTCGATTAGATCATTCGGAAGGACATGATCCTAAAGCTTTAACTCGTTTA
ATCAATCGTCAAACAATTTT

Product: putative acylase and diesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MMIRLYTCIFVFLLNILGSEELKPDLTIMVPMRDGVSLPTDLYLPTPEARNLPCILIRSPAGRDSTWKSFASMAKAGYVI
AIQDTRSVLDLEGKTFPFLSDGWGKLQDGYDAVEWLAKSPYTNGKIGTWGSSAVGITQLLLAPTQPPHLVCQYIIVAAAS
LYHHGLFPGGLLLKHQAESWLGYYARDTGVLNYVSQRPFYNEFWKQLNTSEMAHRVKIPGMLVAGWYDTFLQGTLDAFEA
RQREGEEGAKGKQKLIIGPWTHFWPLSNHFGDFKIPVAGENPPMDISPKRWFDHYLKGEVNEVESLPPVLYYVMGPFDGS
TSSGNIWRTSEVWPVPTVATAFYLTSQHTLQQTPPSLGLLAYTYDPTNPIPTRGGRNLFLESGPVDQQNIEKRKDILVFT
THPLKEDLEITGKLSAKLFVTSDQQDSDIVVHLTDVYPDGKSVLISEGGIRLGVFCYQNDSKIDLKPDQPVEVDLDLWST
SLVFAKGHSIRISISSSNYPRYEKNLNVGLIGSQRGEFKSAHNTIYMGEKYPSQLILPVVRKGETWLTREQPSVKPMENS
L

Sequences:

>Translated_561_residues
MMIRLYTCIFVFLLNILGSEELKPDLTIMVPMRDGVSLPTDLYLPTPEARNLPCILIRSPAGRDSTWKSFASMAKAGYVI
AIQDTRSVLDLEGKTFPFLSDGWGKLQDGYDAVEWLAKSPYTNGKIGTWGSSAVGITQLLLAPTQPPHLVCQYIIVAAAS
LYHHGLFPGGLLLKHQAESWLGYYARDTGVLNYVSQRPFYNEFWKQLNTSEMAHRVKIPGMLVAGWYDTFLQGTLDAFEA
RQREGEEGAKGKQKLIIGPWTHFWPLSNHFGDFKIPVAGENPPMDISPKRWFDHYLKGEVNEVESLPPVLYYVMGPFDGS
TSSGNIWRTSEVWPVPTVATAFYLTSQHTLQQTPPSLGLLAYTYDPTNPIPTRGGRNLFLESGPVDQQNIEKRKDILVFT
THPLKEDLEITGKLSAKLFVTSDQQDSDIVVHLTDVYPDGKSVLISEGGIRLGVFCYQNDSKIDLKPDQPVEVDLDLWST
SLVFAKGHSIRISISSSNYPRYEKNLNVGLIGSQRGEFKSAHNTIYMGEKYPSQLILPVVRKGETWLTREQPSVKPMENS
L
>Mature_561_residues
MMIRLYTCIFVFLLNILGSEELKPDLTIMVPMRDGVSLPTDLYLPTPEARNLPCILIRSPAGRDSTWKSFASMAKAGYVI
AIQDTRSVLDLEGKTFPFLSDGWGKLQDGYDAVEWLAKSPYTNGKIGTWGSSAVGITQLLLAPTQPPHLVCQYIIVAAAS
LYHHGLFPGGLLLKHQAESWLGYYARDTGVLNYVSQRPFYNEFWKQLNTSEMAHRVKIPGMLVAGWYDTFLQGTLDAFEA
RQREGEEGAKGKQKLIIGPWTHFWPLSNHFGDFKIPVAGENPPMDISPKRWFDHYLKGEVNEVESLPPVLYYVMGPFDGS
TSSGNIWRTSEVWPVPTVATAFYLTSQHTLQQTPPSLGLLAYTYDPTNPIPTRGGRNLFLESGPVDQQNIEKRKDILVFT
THPLKEDLEITGKLSAKLFVTSDQQDSDIVVHLTDVYPDGKSVLISEGGIRLGVFCYQNDSKIDLKPDQPVEVDLDLWST
SLVFAKGHSIRISISSSNYPRYEKNLNVGLIGSQRGEFKSAHNTIYMGEKYPSQLILPVVRKGETWLTREQPSVKPMENS
L

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 62855; Mature: 62855

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIRLYTCIFVFLLNILGSEELKPDLTIMVPMRDGVSLPTDLYLPTPEARNLPCILIRSP
CEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECC
AGRDSTWKSFASMAKAGYVIAIQDTRSVLDLEGKTFPFLSDGWGKLQDGYDAVEWLAKSP
CCCCHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHCC
YTNGKIGTWGSSAVGITQLLLAPTQPPHLVCQYIIVAAASLYHHGLFPGGLLLKHQAESW
CCCCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
LGYYARDTGVLNYVSQRPFYNEFWKQLNTSEMAHRVKIPGMLVAGWYDTFLQGTLDAFEA
HHHHHHCCCHHHHHHCCCCHHHHHHHCCHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHH
RQREGEEGAKGKQKLIIGPWTHFWPLSNHFGDFKIPVAGENPPMDISPKRWFDHYLKGEV
HHHCCCCCCCCCCEEEECCCHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCH
NEVESLPPVLYYVMGPFDGSTSSGNIWRTSEVWPVPTVATAFYLTSQHTLQQTPPSLGLL
HHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHEEEEECCHHHHCCCCCCEEE
AYTYDPTNPIPTRGGRNLFLESGPVDQQNIEKRKDILVFTTHPLKEDLEITGKLSAKLFV
EEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEEEE
TSDQQDSDIVVHLTDVYPDGKSVLISEGGIRLGVFCYQNDSKIDLKPDQPVEVDLDLWST
ECCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCEEECCCCCCCEEEEEECCE
SLVFAKGHSIRISISSSNYPRYEKNLNVGLIGSQRGEFKSAHNTIYMGEKYPSQLILPVV
EEEEECCCEEEEEEECCCCCCHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHH
RKGETWLTREQPSVKPMENSL
CCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MMIRLYTCIFVFLLNILGSEELKPDLTIMVPMRDGVSLPTDLYLPTPEARNLPCILIRSP
CEEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCEEEEECC
AGRDSTWKSFASMAKAGYVIAIQDTRSVLDLEGKTFPFLSDGWGKLQDGYDAVEWLAKSP
CCCCHHHHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCCCCCHHHCCCHHHHHHHHHCC
YTNGKIGTWGSSAVGITQLLLAPTQPPHLVCQYIIVAAASLYHHGLFPGGLLLKHQAESW
CCCCCCCCCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHH
LGYYARDTGVLNYVSQRPFYNEFWKQLNTSEMAHRVKIPGMLVAGWYDTFLQGTLDAFEA
HHHHHHCCCHHHHHHCCCCHHHHHHHCCHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHH
RQREGEEGAKGKQKLIIGPWTHFWPLSNHFGDFKIPVAGENPPMDISPKRWFDHYLKGEV
HHHCCCCCCCCCCEEEECCCHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCH
NEVESLPPVLYYVMGPFDGSTSSGNIWRTSEVWPVPTVATAFYLTSQHTLQQTPPSLGLL
HHHHCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCHHHHEEEEECCHHHHCCCCCCEEE
AYTYDPTNPIPTRGGRNLFLESGPVDQQNIEKRKDILVFTTHPLKEDLEITGKLSAKLFV
EEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCEEEEEEEEEEEE
TSDQQDSDIVVHLTDVYPDGKSVLISEGGIRLGVFCYQNDSKIDLKPDQPVEVDLDLWST
ECCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCEEECCCCCCCEEEEEECCE
SLVFAKGHSIRISISSSNYPRYEKNLNVGLIGSQRGEFKSAHNTIYMGEKYPSQLILPVV
EEEEECCCEEEEEEECCCCCCHHCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHH
RKGETWLTREQPSVKPMENSL
CCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]