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The map label for this gene is galE [C]

Identifier: 46445713

GI number: 46445713

Start: 138297

End: 139280

Strand: Direct

Name: galE [C]

Synonym: pc0079

Alternate gene names: 46445713

Gene position: 138297-139280 (Clockwise)

Preceding gene: 46445712

Following gene: 46445714

Centisome position: 5.73

GC content: 35.57

Gene sequence:

>984_bases
ATGTGGAGAGGATTGTCAAAGTTTAAAATGGGAAAACAAATTTTTATTACAGGAATAGCCGGATTCATTGGCTTTCACCT
CGCGCAAAAACTTGCAAAAAGAGGAGATCGTATCATTGGATATGATAATTTTAATCCTTATTATGATACCCAGTTAAAAC
GCGATCGAGCATTAAAGTTATCAAAATTGGGCATTGAAATTATTGAAGGGGATATTCAAAACTATGAAAAGCTGCAAAAT
AGTATCCTATTGCATCAAACGACCCATTTGATCCACTTAGCTGCACAAGCCGGTGTTCGTTATTCATTACAAGAACCGGC
CACTTATCTTAAAACCAATGTAGATGGATTTTTAAATATTCTAGAAATTTGCCGCTCTCATCCCCATTTAAAATTAATTT
ACGCATCCTCGTCTTCCGTTTATGGCCTAAATACGAAAGTTCCTTTCTCTTTAGAAGATCGTACCGATCAACAAGCAAGC
TTATACGGAGTGACTAAGAAAACTAATGAATTAATGGCTAAAACTTATCATCATCTATTTGGAATCTCTTCAATTGGGCT
ACGTTTTTTTACAGTTTATGGGCCTTGGGGTAGGCCAGATATGGCTTATTTCTCGTTTGCAAACGCCATTGTTCAAGGAA
AACCTATTGAAATTTTTAATGAAGGTAAAATGCAAAGAGATTTCACGTATGTGGATGATATTGTGGAAGGAACCATCGGA
GCCATAGATACTGAAATTTCATTAGGCGTTTTTAACTTAGGTAATCACAGACCAGTTGAATTATTATATTTTGTGTTATT
GTTAGAAAAAGAGCTGGGGATTGAAGCTCATAAAATATGGCTGCCCATGCAGTCTGGTGACGTTGTTGCTACCTTTGCTG
ATATCCAGGAAAGCACGAAACAACTTGGCTTTCAACCTAAAATTTCTATTGAAGAAGGCCTTTGTAGATTTGTGAAATGG
TATAAAAACTACTATAATTTATAA

Upstream 100 bases:

>100_bases
AATCCAATGATTGCGGCAGGAACAATGGCAATGAGCTCTGTTTCTGTGATCGCCAATGCGTTATTACTACGTTATTGGAA
GCCAGAAATAAATAGAAATA

Downstream 100 bases:

>100_bases
AAAATTAGGGATTTTATTCATTATTTATTATCAAAGTATTTTTTGATTGGTCGTTATAAGGTTAGCCGGTTATATGTCAA
TTTATTTCGATTCGATATAT

Product: UDP-glucuronat epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MWRGLSKFKMGKQIFITGIAGFIGFHLAQKLAKRGDRIIGYDNFNPYYDTQLKRDRALKLSKLGIEIIEGDIQNYEKLQN
SILLHQTTHLIHLAAQAGVRYSLQEPATYLKTNVDGFLNILEICRSHPHLKLIYASSSSVYGLNTKVPFSLEDRTDQQAS
LYGVTKKTNELMAKTYHHLFGISSIGLRFFTVYGPWGRPDMAYFSFANAIVQGKPIEIFNEGKMQRDFTYVDDIVEGTIG
AIDTEISLGVFNLGNHRPVELLYFVLLLEKELGIEAHKIWLPMQSGDVVATFADIQESTKQLGFQPKISIEEGLCRFVKW
YKNYYNL

Sequences:

>Translated_327_residues
MWRGLSKFKMGKQIFITGIAGFIGFHLAQKLAKRGDRIIGYDNFNPYYDTQLKRDRALKLSKLGIEIIEGDIQNYEKLQN
SILLHQTTHLIHLAAQAGVRYSLQEPATYLKTNVDGFLNILEICRSHPHLKLIYASSSSVYGLNTKVPFSLEDRTDQQAS
LYGVTKKTNELMAKTYHHLFGISSIGLRFFTVYGPWGRPDMAYFSFANAIVQGKPIEIFNEGKMQRDFTYVDDIVEGTIG
AIDTEISLGVFNLGNHRPVELLYFVLLLEKELGIEAHKIWLPMQSGDVVATFADIQESTKQLGFQPKISIEEGLCRFVKW
YKNYYNL
>Mature_327_residues
MWRGLSKFKMGKQIFITGIAGFIGFHLAQKLAKRGDRIIGYDNFNPYYDTQLKRDRALKLSKLGIEIIEGDIQNYEKLQN
SILLHQTTHLIHLAAQAGVRYSLQEPATYLKTNVDGFLNILEICRSHPHLKLIYASSSSVYGLNTKVPFSLEDRTDQQAS
LYGVTKKTNELMAKTYHHLFGISSIGLRFFTVYGPWGRPDMAYFSFANAIVQGKPIEIFNEGKMQRDFTYVDDIVEGTIG
AIDTEISLGVFNLGNHRPVELLYFVLLLEKELGIEAHKIWLPMQSGDVVATFADIQESTKQLGFQPKISIEEGLCRFVKW
YKNYYNL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=321, Percent_Identity=28.6604361370716, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI56237023, Length=339, Percent_Identity=25.3687315634218, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI56118217, Length=339, Percent_Identity=25.3687315634218, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI189083684, Length=339, Percent_Identity=25.3687315634218, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI7657641, Length=325, Percent_Identity=24.3076923076923, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786974, Length=326, Percent_Identity=26.9938650306748, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994969, Length=334, Percent_Identity=22.7544910179641, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788353, Length=338, Percent_Identity=22.4852071005917, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=322, Percent_Identity=27.0186335403727, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982035, Length=338, Percent_Identity=25.7396449704142, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=340, Percent_Identity=25, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=21.5568862275449, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=354, Percent_Identity=24.5762711864407, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19923002, Length=329, Percent_Identity=25.8358662613982, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=28.4403669724771, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37294; Mature: 37294

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRGLSKFKMGKQIFITGIAGFIGFHLAQKLAKRGDRIIGYDNFNPYYDTQLKRDRALKL
CCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHH
SKLGIEIIEGDIQNYEKLQNSILLHQTTHLIHLAAQAGVRYSLQEPATYLKTNVDGFLNI
HHHCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEHHCCHHHHHCCHHHHHHH
LEICRSHPHLKLIYASSSSVYGLNTKVPFSLEDRTDQQASLYGVTKKTNELMAKTYHHLF
HHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHH
GISSIGLRFFTVYGPWGRPDMAYFSFANAIVQGKPIEIFNEGKMQRDFTYVDDIVEGTIG
HHHHHCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCHH
AIDTEISLGVFNLGNHRPVELLYFVLLLEKELGIEAHKIWLPMQSGDVVATFADIQESTK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEHHHHHHHHH
QLGFQPKISIEEGLCRFVKWYKNYYNL
HCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWRGLSKFKMGKQIFITGIAGFIGFHLAQKLAKRGDRIIGYDNFNPYYDTQLKRDRALKL
CCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHH
SKLGIEIIEGDIQNYEKLQNSILLHQTTHLIHLAAQAGVRYSLQEPATYLKTNVDGFLNI
HHHCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEHHCCHHHHHCCHHHHHHH
LEICRSHPHLKLIYASSSSVYGLNTKVPFSLEDRTDQQASLYGVTKKTNELMAKTYHHLF
HHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHHHHHH
GISSIGLRFFTVYGPWGRPDMAYFSFANAIVQGKPIEIFNEGKMQRDFTYVDDIVEGTIG
HHHHHCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCHH
AIDTEISLGVFNLGNHRPVELLYFVLLLEKELGIEAHKIWLPMQSGDVVATFADIQESTK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEHHHHHHHHH
QLGFQPKISIEEGLCRFVKWYKNYYNL
HCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]