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The map label for this gene is panB

Identifier: 45658985

GI number: 45658985

Start: 3873226

End: 3874023

Strand: Reverse

Name: panB

Synonym: LIC13163

Alternate gene names: 45658985

Gene position: 3874023-3873226 (Counterclockwise)

Preceding gene: 45658986

Following gene: 45658984

Centisome position: 90.57

GC content: 39.6

Gene sequence:

>798_bases
ATGAAAAACATACATAAAATTTTTTCTCCTGAAAAGAAAGGGAAAGAAAAAATTTCCGTAGTTACATGTTACGATTTTAG
TTTTGCTAGGATTTTAAACGAAACTGAAATCGATTCTATCCTTGTAGGGGATTCACTGGGAATGGTTTTTCAAGGAAATA
CGAGTACTCTCCCCGTCACTTTAGAAGAAATGATCTATCATACGAAGGCGGTCCGTCGTGGTGCGCCCGATAAGTTTCTG
ATCGCCGATCTACCTTTTTTAAGTTATCAGACTTCTATCGAAGAAGGAATTCGCTCCGCCGGAAAAATCATGAAGGAAAG
CGATTGCGACGCGGTTAAAATAGAAGGTGGTTCCGAATTTATCTGTGAGCTGGTTTCTATTCTCAAACAAATTGGAGTTC
CTGTGATGGGGCATTTGGGGCTGACACCTCAGAGTGTTCATGTGTTTGGCGGTCATCGAGTTCAAGGCAAAGGAGAAGAA
TCGAGTTCTAAACTACTAAAAGAATCGATTTCTCTTTTTGAGTCAGGTGTTTTCTCAATGGTTCTGGAGATGATTCCCGC
TGAACTAGGAAAAAAAGTAAGTCAAGAAGTAGGTGTTCCTACAATCGGAATCGGAGCTGGTTCGGATTGTGACGGACAGG
TTTTGGTTTTAAACGATTTACTTGGATTGGATATAAACTTTCAACCTAAGTTTTTAAAGAAATTTTCCAATCTGCATTCT
ATCGTAAAGGAAGCGATCGCGGATTATGATAAAGAAGTAAAATCCGGAGAGTTTCCAGGGAAAGATCATAGTTTCTGA

Upstream 100 bases:

>100_bases
ACTCTTCCTCATCATTCCCTTTTTACTCGTCCTTTTATCAGAGAGATTTTAGAATCGATCGGCGAACAGTCATTATACGA
GCATTTTACGGGAGGCAAAT

Downstream 100 bases:

>100_bases
CGAGTTCTTGACGATAATCGAAATCATAAAAGGTTGTAAAACTTAAGGGAGATGATCCCGTTTTGGAGTGAAGTGTGGAC
ATAGTTGAGTTGGAAAAGGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKNIHKIFSPEKKGKEKISVVTCYDFSFARILNETEIDSILVGDSLGMVFQGNTSTLPVTLEEMIYHTKAVRRGAPDKFL
IADLPFLSYQTSIEEGIRSAGKIMKESDCDAVKIEGGSEFICELVSILKQIGVPVMGHLGLTPQSVHVFGGHRVQGKGEE
SSSKLLKESISLFESGVFSMVLEMIPAELGKKVSQEVGVPTIGIGAGSDCDGQVLVLNDLLGLDINFQPKFLKKFSNLHS
IVKEAIADYDKEVKSGEFPGKDHSF

Sequences:

>Translated_265_residues
MKNIHKIFSPEKKGKEKISVVTCYDFSFARILNETEIDSILVGDSLGMVFQGNTSTLPVTLEEMIYHTKAVRRGAPDKFL
IADLPFLSYQTSIEEGIRSAGKIMKESDCDAVKIEGGSEFICELVSILKQIGVPVMGHLGLTPQSVHVFGGHRVQGKGEE
SSSKLLKESISLFESGVFSMVLEMIPAELGKKVSQEVGVPTIGIGAGSDCDGQVLVLNDLLGLDINFQPKFLKKFSNLHS
IVKEAIADYDKEVKSGEFPGKDHSF
>Mature_265_residues
MKNIHKIFSPEKKGKEKISVVTCYDFSFARILNETEIDSILVGDSLGMVFQGNTSTLPVTLEEMIYHTKAVRRGAPDKFL
IADLPFLSYQTSIEEGIRSAGKIMKESDCDAVKIEGGSEFICELVSILKQIGVPVMGHLGLTPQSVHVFGGHRVQGKGEE
SSSKLLKESISLFESGVFSMVLEMIPAELGKKVSQEVGVPTIGIGAGSDCDGQVLVLNDLLGLDINFQPKFLKKFSNLHS
IVKEAIADYDKEVKSGEFPGKDHSF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=254, Percent_Identity=46.0629921259843, Blast_Score=247, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6319653, Length=255, Percent_Identity=36.8627450980392, Blast_Score=175, Evalue=8e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_LEPIC (Q72MN0)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_003071.1
- ProteinModelPortal:   Q72MN0
- SMR:   Q72MN0
- GeneID:   2772838
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC13163
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   LINT267671:LIC_13163-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28914; Mature: 28914

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: ACT_SITE 183-183 BINDING 83-83 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIHKIFSPEKKGKEKISVVTCYDFSFARILNETEIDSILVGDSLGMVFQGNTSTLPVT
CCCCHHHCCCCCCCCCEEEEEEEECHHHHHHHCHHHHCEEEECCCCCEEEECCCCCCCEE
LEEMIYHTKAVRRGAPDKFLIADLPFLSYQTSIEEGIRSAGKIMKESDCDAVKIEGGSEF
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
ICELVSILKQIGVPVMGHLGLTPQSVHVFGGHRVQGKGEESSSKLLKESISLFESGVFSM
HHHHHHHHHHHCCCEEECCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VLEMIPAELGKKVSQEVGVPTIGIGAGSDCDGQVLVLNDLLGLDINFQPKFLKKFSNLHS
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECHHCCCCCCCHHHHHHHHHHHH
IVKEAIADYDKEVKSGEFPGKDHSF
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKNIHKIFSPEKKGKEKISVVTCYDFSFARILNETEIDSILVGDSLGMVFQGNTSTLPVT
CCCCHHHCCCCCCCCCEEEEEEEECHHHHHHHCHHHHCEEEECCCCCEEEECCCCCCCEE
LEEMIYHTKAVRRGAPDKFLIADLPFLSYQTSIEEGIRSAGKIMKESDCDAVKIEGGSEF
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
ICELVSILKQIGVPVMGHLGLTPQSVHVFGGHRVQGKGEESSSKLLKESISLFESGVFSM
HHHHHHHHHHHCCCEEECCCCCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
VLEMIPAELGKKVSQEVGVPTIGIGAGSDCDGQVLVLNDLLGLDINFQPKFLKKFSNLHS
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECHHCCCCCCCHHHHHHHHHHHH
IVKEAIADYDKEVKSGEFPGKDHSF
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA