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The map label for this gene is yibK [C]

Identifier: 45658771

GI number: 45658771

Start: 3572786

End: 3573247

Strand: Reverse

Name: yibK [C]

Synonym: LIC12943

Alternate gene names: 45658771

Gene position: 3573247-3572786 (Counterclockwise)

Preceding gene: 45658772

Following gene: 45658770

Centisome position: 83.54

GC content: 38.1

Gene sequence:

>462_bases
ATGTCTTTGTATATAGGTCTTTATAGACCTGAAATTCCTCCTAATACCGGAAACATAGCAAGACTTTGTGTGGCTTTGGG
GGCGGAACTTCATATCGTTGGACAAGCATCTTTTGATCTTTCTGAAAAGGCGGCACGTAGAGCGGGTCTAGATTATTGGG
ATAAGGTGAAAATTTATTTTCATTCTTCTTTAGAAGAATATAAAGTGGCCCTTCCTTCTGGAACCAATCTCTATCTGATT
TCGATTTACGGCAAACGTTCTTACACTAACGTAAGTTATAGGAAAGGGGATGCTTTTTTGTTTGGGAATGAAACTTCTGG
AGTTCCCTTGGAAATAAAAAATTCCTGGGATCAGGAAAGGATTTTAAAAATTCCGATGGAAGATTCTTCCCGTTGTTTGA
ATTTAAGTAATTCAGTCGCAGTAATTTCATACGAAGCGATGCGCCAAATTAAGTCTTGGTAA

Upstream 100 bases:

>100_bases
GATTACAGTAGTTATTTGAGTGTGGACACGAGTGGTTCTACGAGTTCTTTCGGTGCGATCCTTCAAAACTCTTTGAATAA
AAATAAGAAAAAATAATTTT

Downstream 100 bases:

>100_bases
AAACGGATTTACACTTTCAGAATTTCACATCTCAATGGGTAAAGTATGACTTTTCGTCTTTCTAAAGAAGAAATCGAAAA
ACAGATTCGGTCTATGCTTT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSLYIGLYRPEIPPNTGNIARLCVALGAELHIVGQASFDLSEKAARRAGLDYWDKVKIYFHSSLEEYKVALPSGTNLYLI
SIYGKRSYTNVSYRKGDAFLFGNETSGVPLEIKNSWDQERILKIPMEDSSRCLNLSNSVAVISYEAMRQIKSW

Sequences:

>Translated_153_residues
MSLYIGLYRPEIPPNTGNIARLCVALGAELHIVGQASFDLSEKAARRAGLDYWDKVKIYFHSSLEEYKVALPSGTNLYLI
SIYGKRSYTNVSYRKGDAFLFGNETSGVPLEIKNSWDQERILKIPMEDSSRCLNLSNSVAVISYEAMRQIKSW
>Mature_152_residues
SLYIGLYRPEIPPNTGNIARLCVALGAELHIVGQASFDLSEKAARRAGLDYWDKVKIYFHSSLEEYKVALPSGTNLYLIS
IYGKRSYTNVSYRKGDAFLFGNETSGVPLEIKNSWDQERILKIPMEDSSRCLNLSNSVAVISYEAMRQIKSW

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=42.5675675675676, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17291; Mature: 17160

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYIGLYRPEIPPNTGNIARLCVALGAELHIVGQASFDLSEKAARRAGLDYWDKVKIYF
CEEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEE
HSSLEEYKVALPSGTNLYLISIYGKRSYTNVSYRKGDAFLFGNETSGVPLEIKNSWDQER
ECCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCEEEECCCCCCCEEEECCCCCCCC
ILKIPMEDSSRCLNLSNSVAVISYEAMRQIKSW
EEECCCCCCCCEECCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
SLYIGLYRPEIPPNTGNIARLCVALGAELHIVGQASFDLSEKAARRAGLDYWDKVKIYF
EEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEE
HSSLEEYKVALPSGTNLYLISIYGKRSYTNVSYRKGDAFLFGNETSGVPLEIKNSWDQER
ECCCCCEEEECCCCCCEEEEEEECCCCCCCCEEECCCEEEECCCCCCCEEEECCCCCCCC
ILKIPMEDSSRCLNLSNSVAVISYEAMRQIKSW
EEECCCCCCCCEECCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]