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The map label for this gene is pyrB

Identifier: 45658669

GI number: 45658669

Start: 3443599

End: 3444561

Strand: Direct

Name: pyrB

Synonym: LIC12839

Alternate gene names: 45658669

Gene position: 3443599-3444561 (Clockwise)

Preceding gene: 45658668

Following gene: 45658670

Centisome position: 80.51

GC content: 38.21

Gene sequence:

>963_bases
TTGTCAAAAGGGAGTTTTTTTACATTCTGTCGTTTAATGTACTATAATCATAAAAATGTATTAGATACGGAACAGTTCTC
AAAACCAGATCTTGATTTTCTCATTGGGAAAATTAGAGTCATGGAACGTCTTGTAGAACAGAACAAGGCATTTGGAATTC
TTACCGGCAAACTTTTAGCATCTCTTTTTTTTGAAGCCTCTACAAGAACCAGACTTTCTTTCGAAGCGGCTATGGAACGT
TTAGGAGGAAGAGTAATCTCCACGGTGGGATTTCAATTTTCTTCCATTTCCAAAGGAGAAACGTTATACGACACTATGAA
GATGGTGGAAGCTTACGCAGATATTGCTGTCATTAGACATCCTGTAGAAGGTTCTTCCAGAATCGCAGCAGGAGCGGTAA
AAATCCCAGTCATCAACGCTGGAGACGGAGCGGGACAACATCCCACTCAAGCAATTTTAGATCTTTACACGATCATTTCC
GAAAAAGGAACGTTAGACGGTTTGAAGGTTGCGTTTATAGGTGACTTAAAATACGGAAGAACGATCCATTCTCTTATCAA
TTTACTCAGACACTATAAGGTTCGTTTATTTCTGATTTCTCCTCCTGAACTCGCTTTACCAGAATCTTATAAAAAAGCTT
TACAAGGTTATTCTCTTACTCTGGAAGAAACGACAGACATCAAAGCAGTTTGGGATTGTGACGTAGCTTATGTGACTAGA
ATTCAAGAAGAAAGATTTCCAGATCATAAAGAATACGAAAGACTTAAGGATCTTTTTAAGATTAACAAAGAATTAATTCT
TGCTTCCAAAAAAGAAACTACAGTTCTCCATCCTCTTCCTAGGGTGAACGAACTTTCCACGGACGTAGATGATCTACCGA
ACGCGGCTTATTTTAGACAAGCAAGATACGGTGTTGTAAGTCGTATGACTCTTCTTTGTCTTTCTTTAGGACAGGATTTC
TAA

Upstream 100 bases:

>100_bases
CAACGATAGTATGGATCTTTCTTTATGGATACAATCCATTCGACAACTTTCAGGACTTTTAATCCTTCCAGAAAAATCGG
ATCCTCATAAATCAATTTGA

Downstream 100 bases:

>100_bases
TTTTCTTGGAACGGAAACTCTGGACATATCAAGAAGCTCGTAAAATCCTACCTATAGTTAAAGAAATTACGGAAAAATAT
TATTCTTATGTATCGGAATT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSKGSFFTFCRLMYYNHKNVLDTEQFSKPDLDFLIGKIRVMERLVEQNKAFGILTGKLLASLFFEASTRTRLSFEAAMER
LGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVINAGDGAGQHPTQAILDLYTIIS
EKGTLDGLKVAFIGDLKYGRTIHSLINLLRHYKVRLFLISPPELALPESYKKALQGYSLTLEETTDIKAVWDCDVAYVTR
IQEERFPDHKEYERLKDLFKINKELILASKKETTVLHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLSLGQDF

Sequences:

>Translated_320_residues
MSKGSFFTFCRLMYYNHKNVLDTEQFSKPDLDFLIGKIRVMERLVEQNKAFGILTGKLLASLFFEASTRTRLSFEAAMER
LGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVINAGDGAGQHPTQAILDLYTIIS
EKGTLDGLKVAFIGDLKYGRTIHSLINLLRHYKVRLFLISPPELALPESYKKALQGYSLTLEETTDIKAVWDCDVAYVTR
IQEERFPDHKEYERLKDLFKINKELILASKKETTVLHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLSLGQDF
>Mature_319_residues
SKGSFFTFCRLMYYNHKNVLDTEQFSKPDLDFLIGKIRVMERLVEQNKAFGILTGKLLASLFFEASTRTRLSFEAAMERL
GGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVINAGDGAGQHPTQAILDLYTIISE
KGTLDGLKVAFIGDLKYGRTIHSLINLLRHYKVRLFLISPPELALPESYKKALQGYSLTLEETTDIKAVWDCDVAYVTRI
QEERFPDHKEYERLKDLFKINKELILASKKETTVLHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLSLGQDF

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=44.3708609271523, Blast_Score=268, Evalue=6e-72,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=41.9871794871795, Blast_Score=235, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI193204318, Length=284, Percent_Identity=45.0704225352113, Blast_Score=213, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=43.8311688311688, Blast_Score=269, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322373, Length=278, Percent_Identity=26.978417266187, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24642586, Length=302, Percent_Identity=44.3708609271523, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36119; Mature: 35988

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGSFFTFCRLMYYNHKNVLDTEQFSKPDLDFLIGKIRVMERLVEQNKAFGILTGKLLA
CCCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
SLFFEASTRTRLSFEAAMERLGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRH
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PVEGSSRIAAGAVKIPVINAGDGAGQHPTQAILDLYTIISEKGTLDGLKVAFIGDLKYGR
CCCCCCCEEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
TIHSLINLLRHYKVRLFLISPPELALPESYKKALQGYSLTLEETTDIKAVWDCDVAYVTR
HHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHH
IQEERFPDHKEYERLKDLFKINKELILASKKETTVLHPLPRVNELSTDVDDLPNAAYFRQ
HHHHHCCCHHHHHHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHH
ARYGVVSRMTLLCLSLGQDF
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKGSFFTFCRLMYYNHKNVLDTEQFSKPDLDFLIGKIRVMERLVEQNKAFGILTGKLLA
CCCHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
SLFFEASTRTRLSFEAAMERLGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRH
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
PVEGSSRIAAGAVKIPVINAGDGAGQHPTQAILDLYTIISEKGTLDGLKVAFIGDLKYGR
CCCCCCCEEECEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHH
TIHSLINLLRHYKVRLFLISPPELALPESYKKALQGYSLTLEETTDIKAVWDCDVAYVTR
HHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCEEEEECCCHHHHHH
IQEERFPDHKEYERLKDLFKINKELILASKKETTVLHPLPRVNELSTDVDDLPNAAYFRQ
HHHHHCCCHHHHHHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHH
ARYGVVSRMTLLCLSLGQDF
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA