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The map label for this gene is ilvB

Identifier: 45658598

GI number: 45658598

Start: 3369098

End: 3370780

Strand: Reverse

Name: ilvB

Synonym: LIC12764

Alternate gene names: 45658598

Gene position: 3370780-3369098 (Counterclockwise)

Preceding gene: 45658600

Following gene: 45658597

Centisome position: 78.81

GC content: 38.86

Gene sequence:

>1683_bases
ATGAATTTAAACGGAGCTGAACTTATGATTCGGTTTTTGGAATTTGCAGGAGTCGAAATTGTTTCCGGAATTCCAGGGGG
AGCTAGTCTTCCCATCTACGATGCGTTATACGAAAGTAAAATTCGACATATTCTTGCTAGACACGAACAAGGTGCTGGTT
TTATAGCCGGTGGGATGGCAAGAGCAAACGGAAAACCCGGAGTTTGTTTTGCTTCTTCGGGACCAGGAGTTACTAACTTA
ATTACTGCGGTTGCAGACGCGAAAATGGATTCTATTCCTTTGATCGTAATCACCGGGCAGGTTCCGGTTTCTATGATCGG
CACCGACGCATTTCAGGAAATTGATACTTTCAGTTTGTCTGTTCCGATTACTAAAAGAAGTTATCTAGTGAAAAAAATTG
AGGATTTGATTCAAATCCTTCCTCAGGCTTGGAAGACTGCTATTGAAGGTAGGCCAGGTCCGGTTTGGATTGACGTTCCT
AAAGATGTGGCTTCTGCTAAGATTGATTGGGATATTTCCAGAGAAAAAGAAATTTGGAATATTCAAAAAATAGAATTTAA
AGATACGATTGATCTAGAATGGAAAAAAACTTTTAAGGAACTATTATCGAAAGCTAAAAAACCAGTATTTTATATCGGTG
GAGGTTTAAACCGTCCTTTGGCGGCAGAGTTGTTCTGTATTCTTTTGGAAAAACTAAACTTTCCTACCGTTTCTACGTTA
ATGGGATTGGGGATTTGTCCATATGAACATCCTAGATTTTTAGGAATGTTGGGGATGCACGGCTCTAGAGCAGCCAATCT
CGTTTTGGAAGAAGCAGATTTATTGGTTGCTTTGGGCGTTCGTTTTGATGATCGAGCCACTGGTAAATTGAATGAGTTTT
GTCCTAATGCTAAGATAGTCCATGTAGACATAGACGCAACAGAAATCGGTAAATTAAAAAATCCTAATCTTTTTTTAAAA
CACGGCGTTGAGAATTTTATCACCCAGATTTTGGAAGAGGACTTGTCTTCTGAACACTTGTCTTCGGAGGAATGGTTGAA
TCAGGTTCAAACTCTCAAAACTTTATATGGATTTTCTATACCTAAAGAGAATCAAATTTTACATCCGGTTTCGATTTTAC
AAAGTATCTCGAACATTCTAGGTGAAAAGGCAATCATTACTACTGACGTAGGTCAACATCAAATGTGGGTGGCTCAATAT
TATCCTTTTCGAAAGCAAGGTTCTTTTTTGACTTCGGGTGGTTTAGGAACAATGGGTTTTGGATTACCGGCCGCCATTGG
GGCGGCGCTCGTTTTTCCGGAAAAAAGAATCGTTTGTATTTCGGGAGACGGATCGATTTTAATGAATATTCAGGAATTAG
ATACTCTTAGAGAATTGAATTTAGACGTAACTATTCTTTTGATAAACAACGGACATCTTGGTTTGGTGAGACAACAGCAG
GAATTGTTTTTTTCCTCCAGATTTTCCGCATCCAAATTTGTAATACCCGCTAACTTTATAAAAATTGCATCTTCTTTTGG
AATTCCATCTTTTGAGTTGTCGAATGAATGTTCTCAAGTTGAAATTCTGGAACAAGCTTTGATCCGAAAAGGACCATCCT
TTGTTTTGCTTCAGGTAGAGCCCGATCTACAAGTTCTTCCCATGGTTCCTCCAGGAAAGGCAAACCGTGAAATGATTTAT
TAA

Upstream 100 bases:

>100_bases
ACGCAATTCTAAGAAAAGCCTTCTCCGGTGTAAAGCCGGGGGAGGCTTTTTTATTTTTAGGGCTTATCAATGATTTTAAA
GACGATTTAAGGAAACACGT

Downstream 100 bases:

>100_bases
TATATTTATATATTAAAATATAGGAGTTTATTTTATGAAGTTGTATGAGGATTTTAAAACAAAATATTATACTTTTGATA
TTTCTTCCGAAGAAGGTTCT

Product: acetolactate synthase I large subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MNLNGAELMIRFLEFAGVEIVSGIPGGASLPIYDALYESKIRHILARHEQGAGFIAGGMARANGKPGVCFASSGPGVTNL
ITAVADAKMDSIPLIVITGQVPVSMIGTDAFQEIDTFSLSVPITKRSYLVKKIEDLIQILPQAWKTAIEGRPGPVWIDVP
KDVASAKIDWDISREKEIWNIQKIEFKDTIDLEWKKTFKELLSKAKKPVFYIGGGLNRPLAAELFCILLEKLNFPTVSTL
MGLGICPYEHPRFLGMLGMHGSRAANLVLEEADLLVALGVRFDDRATGKLNEFCPNAKIVHVDIDATEIGKLKNPNLFLK
HGVENFITQILEEDLSSEHLSSEEWLNQVQTLKTLYGFSIPKENQILHPVSILQSISNILGEKAIITTDVGQHQMWVAQY
YPFRKQGSFLTSGGLGTMGFGLPAAIGAALVFPEKRIVCISGDGSILMNIQELDTLRELNLDVTILLINNGHLGLVRQQQ
ELFFSSRFSASKFVIPANFIKIASSFGIPSFELSNECSQVEILEQALIRKGPSFVLLQVEPDLQVLPMVPPGKANREMIY

Sequences:

>Translated_560_residues
MNLNGAELMIRFLEFAGVEIVSGIPGGASLPIYDALYESKIRHILARHEQGAGFIAGGMARANGKPGVCFASSGPGVTNL
ITAVADAKMDSIPLIVITGQVPVSMIGTDAFQEIDTFSLSVPITKRSYLVKKIEDLIQILPQAWKTAIEGRPGPVWIDVP
KDVASAKIDWDISREKEIWNIQKIEFKDTIDLEWKKTFKELLSKAKKPVFYIGGGLNRPLAAELFCILLEKLNFPTVSTL
MGLGICPYEHPRFLGMLGMHGSRAANLVLEEADLLVALGVRFDDRATGKLNEFCPNAKIVHVDIDATEIGKLKNPNLFLK
HGVENFITQILEEDLSSEHLSSEEWLNQVQTLKTLYGFSIPKENQILHPVSILQSISNILGEKAIITTDVGQHQMWVAQY
YPFRKQGSFLTSGGLGTMGFGLPAAIGAALVFPEKRIVCISGDGSILMNIQELDTLRELNLDVTILLINNGHLGLVRQQQ
ELFFSSRFSASKFVIPANFIKIASSFGIPSFELSNECSQVEILEQALIRKGPSFVLLQVEPDLQVLPMVPPGKANREMIY
>Mature_560_residues
MNLNGAELMIRFLEFAGVEIVSGIPGGASLPIYDALYESKIRHILARHEQGAGFIAGGMARANGKPGVCFASSGPGVTNL
ITAVADAKMDSIPLIVITGQVPVSMIGTDAFQEIDTFSLSVPITKRSYLVKKIEDLIQILPQAWKTAIEGRPGPVWIDVP
KDVASAKIDWDISREKEIWNIQKIEFKDTIDLEWKKTFKELLSKAKKPVFYIGGGLNRPLAAELFCILLEKLNFPTVSTL
MGLGICPYEHPRFLGMLGMHGSRAANLVLEEADLLVALGVRFDDRATGKLNEFCPNAKIVHVDIDATEIGKLKNPNLFLK
HGVENFITQILEEDLSSEHLSSEEWLNQVQTLKTLYGFSIPKENQILHPVSILQSISNILGEKAIITTDVGQHQMWVAQY
YPFRKQGSFLTSGGLGTMGFGLPAAIGAALVFPEKRIVCISGDGSILMNIQELDTLRELNLDVTILLINNGHLGLVRQQQ
ELFFSSRFSASKFVIPANFIKIASSFGIPSFELSNECSQVEILEQALIRKGPSFVLLQVEPDLQVLPMVPPGKANREMIY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=26.0176991150442, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=26.056338028169, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=50.4472271914132, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI87081685, Length=570, Percent_Identity=41.2280701754386, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=489, Percent_Identity=31.6973415132924, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=27.1095152603232, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=23.4767025089606, Blast_Score=131, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=26.9296740994854, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=539, Percent_Identity=24.4897959183673, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=539, Percent_Identity=24.4897959183673, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=37.6511226252159, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=22.9813664596273, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320123, Length=539, Percent_Identity=22.8200371057514, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320588, Length=527, Percent_Identity=20.6831119544592, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=484, Percent_Identity=22.7272727272727, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323163, Length=480, Percent_Identity=21.4583333333333, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922626, Length=489, Percent_Identity=25.9713701431493, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61656; Mature: 61656

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNGAELMIRFLEFAGVEIVSGIPGGASLPIYDALYESKIRHILARHEQGAGFIAGGMA
CCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCEE
RANGKPGVCFASSGPGVTNLITAVADAKMDSIPLIVITGQVPVSMIGTDAFQEIDTFSLS
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEHHCCHHHHHHCCEEEE
VPITKRSYLVKKIEDLIQILPQAWKTAIEGRPGPVWIDVPKDVASAKIDWDISREKEIWN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCEECCCCCCCCCCCC
IQKIEFKDTIDLEWKKTFKELLSKAKKPVFYIGGGLNRPLAAELFCILLEKLNFPTVSTL
EEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
MGLGICPYEHPRFLGMLGMHGSRAANLVLEEADLLVALGVRFDDRATGKLNEFCPNAKIV
HHCCCCCCCCCHHHHHHCCCCCHHHHEEEECCCEEEEECCCCCCCCCCCHHHHCCCCEEE
HVDIDATEIGKLKNPNLFLKHGVENFITQILEEDLSSEHLSSEEWLNQVQTLKTLYGFSI
EEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PKENQILHPVSILQSISNILGEKAIITTDVGQHQMWVAQYYPFRKQGSFLTSGGLGTMGF
CCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEHHHHCCCCCCCCEEECCCCCCCCC
GLPAAIGAALVFPEKRIVCISGDGSILMNIQELDTLRELNLDVTILLINNGHLGLVRQQQ
CHHHHHHHHEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEHHH
ELFFSSRFSASKFVIPANFIKIASSFGIPSFELSNECSQVEILEQALIRKGPSFVLLQVE
HHHHHCCCCCCEEEECHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEEEC
PDLQVLPMVPPGKANREMIY
CCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNLNGAELMIRFLEFAGVEIVSGIPGGASLPIYDALYESKIRHILARHEQGAGFIAGGMA
CCCCHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCEE
RANGKPGVCFASSGPGVTNLITAVADAKMDSIPLIVITGQVPVSMIGTDAFQEIDTFSLS
CCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEHHCCHHHHHHCCEEEE
VPITKRSYLVKKIEDLIQILPQAWKTAIEGRPGPVWIDVPKDVASAKIDWDISREKEIWN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHCCEECCCCCCCCCCCC
IQKIEFKDTIDLEWKKTFKELLSKAKKPVFYIGGGLNRPLAAELFCILLEKLNFPTVSTL
EEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHH
MGLGICPYEHPRFLGMLGMHGSRAANLVLEEADLLVALGVRFDDRATGKLNEFCPNAKIV
HHCCCCCCCCCHHHHHHCCCCCHHHHEEEECCCEEEEECCCCCCCCCCCHHHHCCCCEEE
HVDIDATEIGKLKNPNLFLKHGVENFITQILEEDLSSEHLSSEEWLNQVQTLKTLYGFSI
EEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
PKENQILHPVSILQSISNILGEKAIITTDVGQHQMWVAQYYPFRKQGSFLTSGGLGTMGF
CCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEHHHHCCCCCCCCEEECCCCCCCCC
GLPAAIGAALVFPEKRIVCISGDGSILMNIQELDTLRELNLDVTILLINNGHLGLVRQQQ
CHHHHHHHHEECCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEHHH
ELFFSSRFSASKFVIPANFIKIASSFGIPSFELSNECSQVEILEQALIRKGPSFVLLQVE
HHHHHCCCCCCEEEECHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEEEC
PDLQVLPMVPPGKANREMIY
CCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]