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The map label for this gene is thiC

Identifier: 45658515

GI number: 45658515

Start: 3246449

End: 3247996

Strand: Direct

Name: thiC

Synonym: LIC12679

Alternate gene names: 45658515

Gene position: 3246449-3247996 (Clockwise)

Preceding gene: 45658514

Following gene: 45658517

Centisome position: 75.9

GC content: 39.86

Gene sequence:

>1548_bases
TTGTTTTTAAGGTTTTCTTTCCGCTTCGGTTCCAACTCCAAACCAACTCCAGGAGAATCTATGGAACCTGTTCAAAAATT
ACAAATTCCTTATAAATCGATCCGACTAAGCGACGGAACCGAATACCAATCCTATCACACCGAAGGTGCCCTCTCTGGAA
AACAACCTGCGGATTATAAAAACGGAATTCCAGCTTTTAGAAAAGAATGGATTCAAAAACGTTTCAATCACTCAAACCAT
TCCCAAATGTATTTTGCTAAAAAAGGAATCATCACGGAAGAAATGCGTTATGCCGCATTTAGGGAGAATATGGAACCAGA
ATTCGTTCGTTCGGAAATCGCCTGTGGTAGAGCCATCTTACCTTCCAATCGGAATCATCCTGAGCTCGAACCGATGGTTA
TCGGTAAAAATTTTCTAGTAAAAATCAACGCAAATATAGGCAACTCCACATTTAGTTCTTCGATCGAAGAAGAAGTCGAA
AAACTACATTGGGCGATCAAATGGGGTGCAGACACAGTTATGGATCTTTCTACGGGAAAAAATATACACGAAACCAGAGA
ATGGATTTTGAGAAATTCACCCGTTCCAATCGGCACCGTTCCTATATATCAAGCCCTAGAAAAAGTAAAAGGAAAAACAG
AAAATTTGAATATTCAAATTTTCTTAGAAACGTTAGAAGAGCAAGCAGAACAAGGGGTAGATTATTTTACAATTCACGCG
GGAGTACTTCTAAGATATATTCCGCTTACTACAAATCGAATAACCGGAATTGTTTCTAGAGGAGGTTCTATACTTGCAAA
ATGGTGCCAGGCACATCACAAAGAGAATTTTTTATATACTCATTTTGACGAAATTCTAAAGGTAATGAAAAAATATGGTG
TTTCCATTTCATTAGGAGATGGCCTTAGACCTGGTTCGATCGCAGATGCAAATGACAAGGCCCAATTTTCAGAATTAGAA
ACCTTGGGAGAACTAACCCAACTTGCTTGGAAAGAAGACATCCAAGTTATGATAGAAGGCCCCGGACATGTTCCTATGAA
TTTAATCAAGGAAAACGTAGATCTTCAAACGAAAATTTGTCAAGAAGCTCCGTTTTATACGTTAGGCCCCATCGTAACAG
ACATAGCTCCCGGTTACGATCATATCACTTCTGCGATCGGTGCTGCTATGATAGGTTGGTACGGAACAGCTATGCTCTGT
TATGTGACTCCCAAAGAACATCTTGGTCTTCCAAATAAAGAAGATGTCAAACAAGGAGTAATCGCATATAAGATCGCCGC
CCACGCTGCCGATTTAGCAAAAGGACATCCCGGAGCAATTGATAGAGATAATCTTTTAAGCAAAGCTCGTTTTGAATTTC
GTTGGGAAGATCAGTTCTCACTTTCCCTGGATCCCGAAACCGCAAAAACATTTCACGACGAAATGCTTCCACAAGATCGA
ATGAAAACGGCTCATTTCTGTTCGATGTGTGGCCCTCATTTCTGCTCTATGAATTTAACTCAAGAACTCAGAAAATTCGC
GCAGGAAAAAGAAATCCAAGAATCCTAA

Upstream 100 bases:

>100_bases
CAGGATAAGACCTGCGTAGGAAAACGGACCTGTAAAATTTATATATTCAAATTTTTGAATAGATTTTCAAATTTTGTCTA
TATAGAATTTTTGCAAAACC

Downstream 100 bases:

>100_bases
GCGATCCAAGTAGGAATTGGAGGAAAAGTCGAAAGAATTTTTGGAACGAAGAACGGAGATCTACGAAAATTCTGAAAGAG
TTTATTTTTAGGATCGGTGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MFLRFSFRFGSNSKPTPGESMEPVQKLQIPYKSIRLSDGTEYQSYHTEGALSGKQPADYKNGIPAFRKEWIQKRFNHSNH
SQMYFAKKGIITEEMRYAAFRENMEPEFVRSEIACGRAILPSNRNHPELEPMVIGKNFLVKINANIGNSTFSSSIEEEVE
KLHWAIKWGADTVMDLSTGKNIHETREWILRNSPVPIGTVPIYQALEKVKGKTENLNIQIFLETLEEQAEQGVDYFTIHA
GVLLRYIPLTTNRITGIVSRGGSILAKWCQAHHKENFLYTHFDEILKVMKKYGVSISLGDGLRPGSIADANDKAQFSELE
TLGELTQLAWKEDIQVMIEGPGHVPMNLIKENVDLQTKICQEAPFYTLGPIVTDIAPGYDHITSAIGAAMIGWYGTAMLC
YVTPKEHLGLPNKEDVKQGVIAYKIAAHAADLAKGHPGAIDRDNLLSKARFEFRWEDQFSLSLDPETAKTFHDEMLPQDR
MKTAHFCSMCGPHFCSMNLTQELRKFAQEKEIQES

Sequences:

>Translated_515_residues
MFLRFSFRFGSNSKPTPGESMEPVQKLQIPYKSIRLSDGTEYQSYHTEGALSGKQPADYKNGIPAFRKEWIQKRFNHSNH
SQMYFAKKGIITEEMRYAAFRENMEPEFVRSEIACGRAILPSNRNHPELEPMVIGKNFLVKINANIGNSTFSSSIEEEVE
KLHWAIKWGADTVMDLSTGKNIHETREWILRNSPVPIGTVPIYQALEKVKGKTENLNIQIFLETLEEQAEQGVDYFTIHA
GVLLRYIPLTTNRITGIVSRGGSILAKWCQAHHKENFLYTHFDEILKVMKKYGVSISLGDGLRPGSIADANDKAQFSELE
TLGELTQLAWKEDIQVMIEGPGHVPMNLIKENVDLQTKICQEAPFYTLGPIVTDIAPGYDHITSAIGAAMIGWYGTAMLC
YVTPKEHLGLPNKEDVKQGVIAYKIAAHAADLAKGHPGAIDRDNLLSKARFEFRWEDQFSLSLDPETAKTFHDEMLPQDR
MKTAHFCSMCGPHFCSMNLTQELRKFAQEKEIQES
>Mature_515_residues
MFLRFSFRFGSNSKPTPGESMEPVQKLQIPYKSIRLSDGTEYQSYHTEGALSGKQPADYKNGIPAFRKEWIQKRFNHSNH
SQMYFAKKGIITEEMRYAAFRENMEPEFVRSEIACGRAILPSNRNHPELEPMVIGKNFLVKINANIGNSTFSSSIEEEVE
KLHWAIKWGADTVMDLSTGKNIHETREWILRNSPVPIGTVPIYQALEKVKGKTENLNIQIFLETLEEQAEQGVDYFTIHA
GVLLRYIPLTTNRITGIVSRGGSILAKWCQAHHKENFLYTHFDEILKVMKKYGVSISLGDGLRPGSIADANDKAQFSELE
TLGELTQLAWKEDIQVMIEGPGHVPMNLIKENVDLQTKICQEAPFYTLGPIVTDIAPGYDHITSAIGAAMIGWYGTAMLC
YVTPKEHLGLPNKEDVKQGVIAYKIAAHAADLAKGHPGAIDRDNLLSKARFEFRWEDQFSLSLDPETAKTFHDEMLPQDR
MKTAHFCSMCGPHFCSMNLTQELRKFAQEKEIQES

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=460, Percent_Identity=68.4782608695652, Blast_Score=678, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 58250; Mature: 58250

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLRFSFRFGSNSKPTPGESMEPVQKLQIPYKSIRLSDGTEYQSYHTEGALSGKQPADYK
CEEEEEEEECCCCCCCCCCCCCHHHHHCCCHHHEEECCCCCHHHHCCCCCCCCCCCCCCC
NGIPAFRKEWIQKRFNHSNHSQMYFAKKGIITEEMRYAAFRENMEPEFVRSEIACGRAIL
CCCHHHHHHHHHHHHCCCCCCEEEEHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEC
PSNRNHPELEPMVIGKNFLVKINANIGNSTFSSSIEEEVEKLHWAIKWGADTVMDLSTGK
CCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCC
NIHETREWILRNSPVPIGTVPIYQALEKVKGKTENLNIQIFLETLEEQAEQGVDYFTIHA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCEEEEHH
GVLLRYIPLTTNRITGIVSRGGSILAKWCQAHHKENFLYTHFDEILKVMKKYGVSISLGD
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECC
GLRPGSIADANDKAQFSELETLGELTQLAWKEDIQVMIEGPGHVPMNLIKENVDLQTKIC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCHHHHHH
QEAPFYTLGPIVTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKEDVKQGV
HCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHH
IAYKIAAHAADLAKGHPGAIDRDNLLSKARFEFRWEDQFSLSLDPETAKTFHDEMLPQDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEECCCEEEEECCHHHHHHHHHHCCHHH
MKTAHFCSMCGPHFCSMNLTQELRKFAQEKEIQES
HHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLRFSFRFGSNSKPTPGESMEPVQKLQIPYKSIRLSDGTEYQSYHTEGALSGKQPADYK
CEEEEEEEECCCCCCCCCCCCCHHHHHCCCHHHEEECCCCCHHHHCCCCCCCCCCCCCCC
NGIPAFRKEWIQKRFNHSNHSQMYFAKKGIITEEMRYAAFRENMEPEFVRSEIACGRAIL
CCCHHHHHHHHHHHHCCCCCCEEEEHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCEEC
PSNRNHPELEPMVIGKNFLVKINANIGNSTFSSSIEEEVEKLHWAIKWGADTVMDLSTGK
CCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCC
NIHETREWILRNSPVPIGTVPIYQALEKVKGKTENLNIQIFLETLEEQAEQGVDYFTIHA
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCCCCEEEEHH
GVLLRYIPLTTNRITGIVSRGGSILAKWCQAHHKENFLYTHFDEILKVMKKYGVSISLGD
HHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECC
GLRPGSIADANDKAQFSELETLGELTQLAWKEDIQVMIEGPGHVPMNLIKENVDLQTKIC
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCHHHHHH
QEAPFYTLGPIVTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKEDVKQGV
HCCCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCHHHHHHHH
IAYKIAAHAADLAKGHPGAIDRDNLLSKARFEFRWEDQFSLSLDPETAKTFHDEMLPQDR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCEEEECCCEEEEECCHHHHHHHHHHCCHHH
MKTAHFCSMCGPHFCSMNLTQELRKFAQEKEIQES
HHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA