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The map label for this gene is rho

Identifier: 45658473

GI number: 45658473

Start: 3195007

End: 3196455

Strand: Direct

Name: rho

Synonym: LIC12636

Alternate gene names: 45658473

Gene position: 3195007-3196455 (Clockwise)

Preceding gene: 45658472

Following gene: 45658474

Centisome position: 74.7

GC content: 42.93

Gene sequence:

>1449_bases
ATGGCAACAGCAAGACGAGACAATAAAAACAGACACCAACATTCCAACCACAATCATAACAACCAAAACGATTCTGGAGA
TTCTTTCGAAGAAGAAAGTACAAACCAAGAATCTCAAGAATTTGATTCTTCCGAAAATTCAGATCCCAGATCTCGTAAAC
GCAAACGAGGGGGTTACGAAGGCCCCGCCCCTGCTCCTATCGATCTAGTAGAACTGAAGAAAAAAGCAATTTCTGATTTG
ATCGAAGTCGCCAAAAGTTTAGGAGTAGAAAATACCGGCGGTTTAAAAAAGCAGAATTTGATCTTTGCCATTTTACAAGC
GCAAGCGGAAAGAGACGGACAAGTCCACGCCGCCGGGGTTATGGAAAAACTTCCGGACGGATATGGATTTTTACGTTCTC
CGGATTACAACTACGTGCCGGGTCCGGATGACATTTATGTTTCTCCTTCTCAGATCAAATTGTTCGGTCTTAGAACCGGG
GATACGGTAGAAGGTCAAATTCGTCCTCCCAAAGAATCGGAACGATTTTTTGCCATGCTCCGAGTGGAAACCGTCAACAG
TTATACTCCGGACGTAGCCGGTAAACGCGCCTTATTTGACAACTTAACACCTCTTTATCCAAACGAAAGACTCAAGATGG
AATACGATCCATCGATGCTCGACACTAGAATCTTAGATTTAATGTGTCCAATTGGAAAAGGACAAAGGGCTTTGATCGTA
GCACCTCCCAGAACCGGTAAAACGATTTTGATGCAGAATATCGCAAACGCGATTACATCCAATCATCCGGAATGTTCTTT
AATTGTACTTCTGATCGACGAACGTCCCGAAGAAGTGACAGACATGGCCCGTCACGTTCGGGGAGAAGTAGTTTCTTCCA
CATTTGACGAACCTGCACAAAGACACGTTCAAGTCGCCGAGATGGTCATCGAAAAAGCAAAACGTCTCGTCGAACATGGA
AAAGACGTGGTTATACTTTTAGATTCGATCACTCGTTTGGCTCGGGCCTACAATCAAGTCATTCCAACTTCCGGTAAAAT
TCTTTCCGGTGGAGTGGACTCCAATGCACTTCATAAACCAAAACGATTTTTTGGAGCCGCAAGAAACATTGAAGAAGGTG
GTTCTCTCACGATCATCGCCACTGCTTTGATAGATACTGGATCCAAGATGGACGAAGTAATCTTTGAAGAATTCAAAGGA
ACTGGAAATATGGAGATCCATCTGGATCGGAAACTTTCTGATAAACGGATCTTCCCTGCAATCGATATTAATAAATCCGG
AACTCGTAAAGAAGAACTACTAATTACCAAAGACGTCCTTCAGAAAGTGTTTGTTCTGCGAAAAGTACTTTCTCCTATGA
GTATCACGGAAAGCATGGAATTATTGCTGGAAAAAATGAGGCTTTCTAAAACAAACGACGCCTTTTTAGCCAGTATGAAC
ACACAGTAA

Upstream 100 bases:

>100_bases
AGCCGATCTAACTGATCTCTTCTCCACTCTGAATTCTCAAAAATAAAATCTCAAAAAGGCGAAATCCTTCTCCCTTTTTA
AAACTCAAGTAGTTCTTAAT

Downstream 100 bases:

>100_bases
ACGTTTCAACAAGGGAATTTATGAAAACTGAAATTCATCCAAACTATAAGGCGGCTAAAATCAGCTGTGCATCCTGTGGA
ACCGTATACGAAACCAGAAC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MATARRDNKNRHQHSNHNHNNQNDSGDSFEEESTNQESQEFDSSENSDPRSRKRKRGGYEGPAPAPIDLVELKKKAISDL
IEVAKSLGVENTGGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTG
DTVEGQIRPPKESERFFAMLRVETVNSYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIV
APPRTGKTILMQNIANAITSNHPECSLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHG
KDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKG
TGNMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMN
TQ

Sequences:

>Translated_482_residues
MATARRDNKNRHQHSNHNHNNQNDSGDSFEEESTNQESQEFDSSENSDPRSRKRKRGGYEGPAPAPIDLVELKKKAISDL
IEVAKSLGVENTGGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTG
DTVEGQIRPPKESERFFAMLRVETVNSYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIV
APPRTGKTILMQNIANAITSNHPECSLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHG
KDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKG
TGNMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMN
TQ
>Mature_481_residues
ATARRDNKNRHQHSNHNHNNQNDSGDSFEEESTNQESQEFDSSENSDPRSRKRKRGGYEGPAPAPIDLVELKKKAISDLI
EVAKSLGVENTGGLKKQNLIFAILQAQAERDGQVHAAGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGD
TVEGQIRPPKESERFFAMLRVETVNSYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIVA
PPRTGKTILMQNIANAITSNHPECSLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQRHVQVAEMVIEKAKRLVEHGK
DVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGT
GNMEIHLDRKLSDKRIFPAIDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMNT
Q

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=64.9038461538462, Blast_Score=569, Evalue=1e-163,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 53791; Mature: 53660

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATARRDNKNRHQHSNHNHNNQNDSGDSFEEESTNQESQEFDSSENSDPRSRKRKRGGYE
CCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCHHHHHHHCCCCC
GPAPAPIDLVELKKKAISDLIEVAKSLGVENTGGLKKQNLIFAILQAQAERDGQVHAAGV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
MEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVEGQIRPPKESERFFAML
HHHCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCHHHHEEEHE
RVETVNSYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIV
EEEHHCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEE
APPRTGKTILMQNIANAITSNHPECSLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQ
ECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHH
RHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCH
KRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIHLDRKLSDKRIFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
IDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECC
TQ
CC
>Mature Secondary Structure 
ATARRDNKNRHQHSNHNHNNQNDSGDSFEEESTNQESQEFDSSENSDPRSRKRKRGGYE
CCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHCCCCCCCHHHHHHHCCCCC
GPAPAPIDLVELKKKAISDLIEVAKSLGVENTGGLKKQNLIFAILQAQAERDGQVHAAGV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
MEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTVEGQIRPPKESERFFAML
HHHCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCCCCCHHHHEEEHE
RVETVNSYTPDVAGKRALFDNLTPLYPNERLKMEYDPSMLDTRILDLMCPIGKGQRALIV
EEEHHCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEE
APPRTGKTILMQNIANAITSNHPECSLIVLLIDERPEEVTDMARHVRGEVVSSTFDEPAQ
ECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHH
RHVQVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNQVIPTSGKILSGGVDSNALHKP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCH
KRFFGAARNIEEGGSLTIIATALIDTGSKMDEVIFEEFKGTGNMEIHLDRKLSDKRIFPA
HHHHHHHCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
IDINKSGTRKEELLITKDVLQKVFVLRKVLSPMSITESMELLLEKMRLSKTNDAFLASMN
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEECC
TQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]