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The map label for this gene is miaB [H]

Identifier: 45658409

GI number: 45658409

Start: 3116548

End: 3117897

Strand: Reverse

Name: miaB [H]

Synonym: LIC12570

Alternate gene names: 45658409

Gene position: 3117897-3116548 (Counterclockwise)

Preceding gene: 45658419

Following gene: 45658408

Centisome position: 72.9

GC content: 39.78

Gene sequence:

>1350_bases
ATGAGTGTTCTGGAACAGGAAAAAAAGTCAGGAAAAGTCTATATCGAGACTTATGGCTGTCAGATGAATGAATATGACTC
TGGGATCGTTTCCAGTTTAATGAGAGACGCAGAGTATTCTACCTCTTCCGACCCGGAGAATTCCGACATTATTTTCTTAA
ATACCTGTGCGATTCGGGAAAACGCTCATGCAAAAATCTACAATCGCCTTCAATCTTTAGGATATTTGAAAAAAAGAAAT
CCGAATCTGGTGATTGGGGTTTTAGGTTGTATGGCTCAGAATTTGGGAGACGACCTATTTCATCAGGAACTTCCTCTGGA
TTTGGTCGTAGGACCGGACAATTATAGAAGTCTTCCAGAGCTCATCCAAAGAATTCGGAAAGGAGAAAACTCGATTTCTC
TAACCCGACTTTCTAAAATCGAAACCTACGACGAAATAGAACCTAGGGTAGTTAACGGAATTCAAGCCTTCGTTACGATT
ATGAGAGGTTGTAATAATTTTTGTACTTTTTGTGTGGTTCCTTATACTCGAGGAAGAGAAAGAAGTAGAGATCCTAAAAG
TATTGTTCGAGAAGTTCAAGATCTAGTACAAAAGGGGATTCGTCAGATAACTCTTCTTGGACAAAACGTAAATTCTTACA
AAGAACAAGATACTGATTTTGCGGGTTTGATCCAAATGCTTTTAGATGAAACTTCCATTGAAAGAATTCGTTTTACTTCT
CCCCATCCAAAGGATTTTCCGACCCATTTACTTCAATTGATGTCTGAGAATCCACGTTTTTGTCCAAACATTCATCTGCC
TTTGCAAGCTGGAAACACTAGAGTTTTAGAAGAGATGAAACGTTCTTATTCCAAAGAAGAATTTTTAGATGTAGTCAAAG
AAATTAGAAATATAGTTCCTGATGTGGGAATTACAACAGACATCATCGTAGGTTTTCCGAATGAAACAGAAGAGGAATTT
GAGGATACGCTTGCGGTCGTTCGGGAAGTCCAATTTGATATGGCATTTATGTTCAAGTATTCTGAAAGAGAAGGAACGAT
GGCTCAAAGAAAGCTTCCGGATAACGTTCCCGAAGAAGTGAAGTCTGCAAGACTTACAAAACTGGTAGATTTACAAACTT
CGATTTCTCACGAACAGAACCGCGCCCGGATCGGAAGAGTATATTCCATTTTGATTGAGAACATTTCCCGTAAGTCGGAA
AAACAACTCTGTGGAAGAACTCCTTGTGGAAGAATGACGGTGTTTCCTCTTCCGCAAGAAACAAACGTTTCCGGTATGAT
CGGTTCTACGGTTTCTGTCCAAATCGAAAGCGCAACCAGCGCTACTCTCAAAGGTAGGATCCTTGCTTGA

Upstream 100 bases:

>100_bases
TAAAAATTCTGGAACGTAGGATTTTGCTTCTAAAAATCCTGATTTTCCTATCTATGGAAAACCCCTTTACAGTCCGTCCT
GCAAGCAGAAATTCAGTTCT

Downstream 100 bases:

>100_bases
AGGATGGCGACAATCGCAAGAAGATTTCTGCTAAAAAGTCAACGACGCGTGCGTCCTCAAAACAAACAACAAATTCTTCT
AAGAAAAATCAGAACTACTC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRN
PNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTI
MRGCNNFCTFCVVPYTRGRERSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTS
PHPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVPDVGITTDIIVGFPNETEEEF
EDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEVKSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSE
KQLCGRTPCGRMTVFPLPQETNVSGMIGSTVSVQIESATSATLKGRILA

Sequences:

>Translated_449_residues
MSVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRN
PNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTI
MRGCNNFCTFCVVPYTRGRERSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTS
PHPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVPDVGITTDIIVGFPNETEEEF
EDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEVKSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSE
KQLCGRTPCGRMTVFPLPQETNVSGMIGSTVSVQIESATSATLKGRILA
>Mature_448_residues
SVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRNP
NLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTIM
RGCNNFCTFCVVPYTRGRERSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTSP
HPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVPDVGITTDIIVGFPNETEEEFE
DTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEVKSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSEK
QLCGRTPCGRMTVFPLPQETNVSGMIGSTVSVQIESATSATLKGRILA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=41.3865546218487, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=41.3865546218487, Blast_Score=371, Evalue=1e-103,
Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=25.9445843828715, Blast_Score=133, Evalue=4e-31,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=38.2022471910112, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=28.9183222958057, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=38.5775862068966, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71996771, Length=318, Percent_Identity=25.1572327044025, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=40.7172995780591, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922432, Length=449, Percent_Identity=27.3942093541203, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50989; Mature: 50858

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRE
CCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
NAHAKIYNRLQSLGYLKKRNPNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPE
CCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCHHHHHHCCCEEEEECCCCCCHHHH
LIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRE
HHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTS
CCCCHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECC
PHPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVP
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
DVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCHHHH
KSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSEKQLCGRTPCGRMTVFPLPQE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
TNVSGMIGSTVSVQIESATSATLKGRILA
CCCCCEECCEEEEEEECCCCHHHCCCCCC
>Mature Secondary Structure 
SVLEQEKKSGKVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRE
CCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
NAHAKIYNRLQSLGYLKKRNPNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPE
CCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCHHHHHHCCCEEEEECCCCCCHHHH
LIQRIRKGENSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRE
HHHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
RSRDPKSIVREVQDLVQKGIRQITLLGQNVNSYKEQDTDFAGLIQMLLDETSIERIRFTS
CCCCHHHHHHHHHHHHHHHHHHEEHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHEEECC
PHPKDFPTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVP
CCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
DVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEV
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHCCCCCCHHHH
KSARLTKLVDLQTSISHEQNRARIGRVYSILIENISRKSEKQLCGRTPCGRMTVFPLPQE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
TNVSGMIGSTVSVQIESATSATLKGRILA
CCCCCEECCEEEEEEECCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA