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The map label for this gene is risA

Identifier: 45658377

GI number: 45658377

Start: 3073779

End: 3074381

Strand: Reverse

Name: risA

Synonym: LIC12535

Alternate gene names: 45658377

Gene position: 3074381-3073779 (Counterclockwise)

Preceding gene: 45658378

Following gene: 45658376

Centisome position: 71.88

GC content: 37.65

Gene sequence:

>603_bases
ATGTTTACCGGTTTAGTCGAAACCACTGGAAGAATTTCTGAAATTCAAGAAACGACTGAGGGAAGAAGTTTTTTAGTGGA
GACAAAGTGGGTGCGTCCGGATCTCAAGTTAGGTGACTCGATTGCAGTGAATGGTTGTTGCCAGACCGTTACTTCGTTTT
ACGAAGAGGGGGATCGTTTCCGATTTTATGCTAGTTTTAAAACTTTAGAATTAACTAATTTTAAGTTTTTTAAAGTAGGA
GAAGAAGTAAATTTAGAGCGGAGTGTACTTCCTACAACTCGTTTAGGTGGGCATCTTGTAAGTGGTCATGTGGATGGGGT
TGGAAAAATTCTGAACAAAGAAGAAAGAGAAGATGGAAATGTGATTTGTTATACGGTCCGAAATGATTCTTCTCTTTCGA
AATACATCGCTCCGAGAGGAAGTATTACTGTGGATGGAATTTCGTTGACCGTGGTTAATTCCCATTCTGATACGTTTGAT
TTGGTTTTGATTCCAGAAACTCTTAAAAAAACGAATGCTAAATCTTGGAATTCAGACTCGGTTTTAAATTTAGAGATAGA
TCTTGTAGCTCGCTATTTGGAACAACTTCTTAAGTTTCAGTAA

Upstream 100 bases:

>100_bases
CTTTTACCATACCGGAAGGTATAAAACCCAAGTTGATTACAAATTCTGAAACCTTAATCTGGAAAACAAGTGTCGGGAAA
GATATTTGGGAGGTGCACGG

Downstream 100 bases:

>100_bases
AGTAATAGTTCCTACATTTTCAGAGTTTATCCGTAAAATTGAGATTTTGTAAGAGTTCCCACGTTTTCCACTGTGAAATA
AATCTTTAAATGGATTTTTT

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MFTGLVETTGRISEIQETTEGRSFLVETKWVRPDLKLGDSIAVNGCCQTVTSFYEEGDRFRFYASFKTLELTNFKFFKVG
EEVNLERSVLPTTRLGGHLVSGHVDGVGKILNKEEREDGNVICYTVRNDSSLSKYIAPRGSITVDGISLTVVNSHSDTFD
LVLIPETLKKTNAKSWNSDSVLNLEIDLVARYLEQLLKFQ

Sequences:

>Translated_200_residues
MFTGLVETTGRISEIQETTEGRSFLVETKWVRPDLKLGDSIAVNGCCQTVTSFYEEGDRFRFYASFKTLELTNFKFFKVG
EEVNLERSVLPTTRLGGHLVSGHVDGVGKILNKEEREDGNVICYTVRNDSSLSKYIAPRGSITVDGISLTVVNSHSDTFD
LVLIPETLKKTNAKSWNSDSVLNLEIDLVARYLEQLLKFQ
>Mature_200_residues
MFTGLVETTGRISEIQETTEGRSFLVETKWVRPDLKLGDSIAVNGCCQTVTSFYEEGDRFRFYASFKTLELTNFKFFKVG
EEVNLERSVLPTTRLGGHLVSGHVDGVGKILNKEEREDGNVICYTVRNDSSLSKYIAPRGSITVDGISLTVVNSHSDTFD
LVLIPETLKKTNAKSWNSDSVLNLEIDLVARYLEQLLKFQ

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=192, Percent_Identity=38.5416666666667, Blast_Score=110, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319733, Length=209, Percent_Identity=33.9712918660287, Blast_Score=101, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22453; Mature: 22453

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGLVETTGRISEIQETTEGRSFLVETKWVRPDLKLGDSIAVNGCCQTVTSFYEEGDRF
CCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCEE
RFYASFKTLELTNFKFFKVGEEVNLERSVLPTTRLGGHLVSGHVDGVGKILNKEEREDGN
EEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCC
VICYTVRNDSSLSKYIAPRGSITVDGISLTVVNSHSDTFDLVLIPETLKKTNAKSWNSDS
EEEEEEECCCCHHHHHCCCCCEEECCEEEEEEECCCCEEEEEEECHHHHHCCCCCCCCCC
VLNLEIDLVARYLEQLLKFQ
EEEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTGLVETTGRISEIQETTEGRSFLVETKWVRPDLKLGDSIAVNGCCQTVTSFYEEGDRF
CCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEHHHHHHHHHHHHCCCEE
RFYASFKTLELTNFKFFKVGEEVNLERSVLPTTRLGGHLVSGHVDGVGKILNKEEREDGN
EEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCC
VICYTVRNDSSLSKYIAPRGSITVDGISLTVVNSHSDTFDLVLIPETLKKTNAKSWNSDS
EEEEEEECCCCHHHHHCCCCCEEECCEEEEEEECCCCEEEEEEECHHHHHCCCCCCCCCC
VLNLEIDLVARYLEQLLKFQ
EEEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339274 [H]