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The map label for this gene is carA

Identifier: 45658308

GI number: 45658308

Start: 2983819

End: 2984910

Strand: Direct

Name: carA

Synonym: LIC12466

Alternate gene names: 45658308

Gene position: 2983819-2984910 (Clockwise)

Preceding gene: 45658306

Following gene: 45658309

Centisome position: 69.76

GC content: 36.72

Gene sequence:

>1092_bases
TTGATGAAAGCATTCTTAGTTCTTGATAACGGAACAATCTTCGAAGGTGAATCCTTCGGTTATGAAACTGAATCCGTTGG
AGAAATTGTCTTTAATACTTCTATGGCGGGTTATCAAGAGATTTTAACGGACCCTTCTTACTGTAATCAGATCATTACAC
TGACTTATCCTATGATCGGAAATTACGGAATCCATCCTGATAACATGGAATCTTCTAAAATTCAAGCCAGCGGTTTAATC
GTTAAAGAATACGTAGATCTACCTTCTAACTTTAAATCCGAAAAAACGTTATCTCAGTTTTTAAAAGAATATAAAATTCC
AGCCATTCAGGGGATAGACACTCGTAAATTGACCCGGTTTATACGCACAAACGGTTCTCCCAATGGTGGAATTTTTGTGG
CTTCGGAATATTCTCCTTCTTTTTTAGAAAAGGTAAAATCATTTCCAGGAATCATCAACGCAGATCTAGCTGAGGTAGTA
ACTACTTCCAGTAAATATATCTTTGGAACTCACACTGGTAAAAAATTTAAACTCGCAGTATATGACTATGGCGTGAAAAC
AAACATACTACGTCTTTTAGATGCGAATGGTTTTGCAGTAACAGTTTATCCAGCAAAAACTCCTTCAGAAGAAATTATGA
AAGAAGGAACCGACGCATTTTTTCTTTCTAATGGCCCTGGAGACCCCGCCCCCTTAGATTACGCGATTGCTTCAACTCAA
AAGATTATGGAAAAAAGATATCCTCTTTTTGGAATCTGTCTGGGTCATCAAATCATTGGGCTTTCTTTAGGAAAAAAAAC
GGAAAAGATGAAATTCGGTCATAGAGGTGGAAACCAACCGGTCAAAAATCTTGAGACTGGTCAGGTAGAAATTACTTCGC
AAAATCATGGTTTTGCGGTAATAGACGACCAGAAACAGGATGAACCAATTTCTTTTCTGAACCTAAACGATCATACTGTA
GAAGGAATTTTAAAATCTGGTTATCCTCTATTGACCGTTCAGTATCATCCGGAAAGTGCTCCTGGTCCAAACGATTCACG
GTATCTATTTCAAAAATTTTATGATTTAGTAGAAAAAACAAAAAAAGGTTAA

Upstream 100 bases:

>100_bases
TACTCTCCATTCACAAAAATCGTAGGTATTTTTCAACCGATTTGTAAGGACTCAAAAAAAACCTGTGTTGACTATACTCA
ATACCTCATTGAGTTTGTAA

Downstream 100 bases:

>100_bases
AACTTATGACAGAATTTACACAACTCCCCGACAGAATTTTAGGACTTCCCGTTGGCAAAGATTTTTCCATAGAGGCTTTG
GTCAAAGACGTTTGGAATCC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMKAFLVLDNGTIFEGESFGYETESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLI
VKEYVDLPSNFKSEKTLSQFLKEYKIPAIQGIDTRKLTRFIRTNGSPNGGIFVASEYSPSFLEKVKSFPGIINADLAEVV
TTSSKYIFGTHTGKKFKLAVYDYGVKTNILRLLDANGFAVTVYPAKTPSEEIMKEGTDAFFLSNGPGDPAPLDYAIASTQ
KIMEKRYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLETGQVEITSQNHGFAVIDDQKQDEPISFLNLNDHTV
EGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVEKTKKG

Sequences:

>Translated_363_residues
MMKAFLVLDNGTIFEGESFGYETESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLI
VKEYVDLPSNFKSEKTLSQFLKEYKIPAIQGIDTRKLTRFIRTNGSPNGGIFVASEYSPSFLEKVKSFPGIINADLAEVV
TTSSKYIFGTHTGKKFKLAVYDYGVKTNILRLLDANGFAVTVYPAKTPSEEIMKEGTDAFFLSNGPGDPAPLDYAIASTQ
KIMEKRYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLETGQVEITSQNHGFAVIDDQKQDEPISFLNLNDHTV
EGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVEKTKKG
>Mature_363_residues
MMKAFLVLDNGTIFEGESFGYETESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLI
VKEYVDLPSNFKSEKTLSQFLKEYKIPAIQGIDTRKLTRFIRTNGSPNGGIFVASEYSPSFLEKVKSFPGIINADLAEVV
TTSSKYIFGTHTGKKFKLAVYDYGVKTNILRLLDANGFAVTVYPAKTPSEEIMKEGTDAFFLSNGPGDPAPLDYAIASTQ
KIMEKRYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLETGQVEITSQNHGFAVIDDQKQDEPISFLNLNDHTV
EGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVEKTKKG

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=373, Percent_Identity=40.4825737265416, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI21361331, Length=371, Percent_Identity=38.544474393531, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI169790915, Length=371, Percent_Identity=38.544474393531, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=47.2295514511873, Blast_Score=348, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI193204318, Length=378, Percent_Identity=40.4761904761905, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=375, Percent_Identity=38.4, Blast_Score=247, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=37.0843989769821, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24642586, Length=381, Percent_Identity=40.1574803149606, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=40.1574803149606, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40262; Mature: 40262

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAFLVLDNGTIFEGESFGYETESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIG
CCEEEEEEECCEEECCCCCCCCCHHHCCEEEECCHHHHHHHHCCHHHHCEEEEEEECCCC
NYGIHPDNMESSKIQASGLIVKEYVDLPSNFKSEKTLSQFLKEYKIPAIQGIDTRKLTRF
CCCCCCCCCCCCEEEECCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRTNGSPNGGIFVASEYSPSFLEKVKSFPGIINADLAEVVTTSSKYIFGTHTGKKFKLAV
HHCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEE
YDYGVKTNILRLLDANGFAVTVYPAKTPSEEIMKEGTDAFFLSNGPGDPAPLDYAIASTQ
EECCCHHHEEEEEECCCEEEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KIMEKRYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLETGQVEITSQNHGFAV
HHHHHCCCEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
IDDQKQDEPISFLNLNDHTVEGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVEKT
ECCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KKG
CCC
>Mature Secondary Structure
MMKAFLVLDNGTIFEGESFGYETESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIG
CCEEEEEEECCEEECCCCCCCCCHHHCCEEEECCHHHHHHHHCCHHHHCEEEEEEECCCC
NYGIHPDNMESSKIQASGLIVKEYVDLPSNFKSEKTLSQFLKEYKIPAIQGIDTRKLTRF
CCCCCCCCCCCCEEEECCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRTNGSPNGGIFVASEYSPSFLEKVKSFPGIINADLAEVVTTSSKYIFGTHTGKKFKLAV
HHCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEECCCCEEEEEE
YDYGVKTNILRLLDANGFAVTVYPAKTPSEEIMKEGTDAFFLSNGPGDPAPLDYAIASTQ
EECCCHHHEEEEEECCCEEEEEEECCCCHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
KIMEKRYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLETGQVEITSQNHGFAV
HHHHHCCCEEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
IDDQKQDEPISFLNLNDHTVEGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVEKT
ECCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
KKG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA