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The map label for this gene is cheB1

Identifier: 45658298

GI number: 45658298

Start: 2971890

End: 2972963

Strand: Reverse

Name: cheB1

Synonym: LIC12455

Alternate gene names: 45658298

Gene position: 2972963-2971890 (Counterclockwise)

Preceding gene: 45658299

Following gene: 45658297

Centisome position: 69.51

GC content: 41.34

Gene sequence:

>1074_bases
ATGATTCCAAATCCGGTTCGTGCGGTTATTATCGATGATTCTCTATTAGTCAGAAATATCATCTCTGATCAGATCCAAAA
AGATTCTAAGATCCATGTTGTCGCAACCGGCAAAACCGGAATGGATTGTATTGATCTTGCTCAGAAGATGAATCCGGACG
TGATTATACTCGACGTAGAAATGCCGGTTATGGACGGCCTTACGGCACTTCATGAACTTCAAAAAAAGAAATTAGGAATT
CCTGTCATTATGCTTTCCGTTTTGACTCAGAATGGAGCCGAAGCAACTTTCAAGGCTTTGGAATATGGAGCCATTGATTT
TGTACCGAAACCTTCCAGTGTGTTTCAATTCGATCCGGAAGAAATTGGTAATATTTTAAAATCTAAAATTTTAGCTTACT
TCGAAAGTAAAATACAAATTCCTTCCCAAAATCTTTTAAAAAAGGCTCCGGCTTATTCAAAGGTTCCAGCTGGATCGCAT
TTAAAAAAATCCCCGATTCAAGCCATTTGTATAGGTACTTCCACTGGAGGACCTAGAGCCCTTCAAGAAGTGTTTTCCAG
AATTCCAGCGGACATTTCTTTACCGATTTTAGTCGTTCAACATATGCCCGCGGGTTTTACAAAAGCGTTTGCGACTAGGC
TGAACGATCACGCAAAAATCAAAGTAAAAGAAGCAGAGGATGGTGAGCCGATAGAACCGAACACTGGTTATGTGGCACCG
GGTGACGCACATCTTTCCATTCAATCCAAAGGAGGGAGGAAATGGATTGCCCTGAACAGGGAAGCCCCTGTAAATGGACA
CAGACCTTCCATCGAGGTTCTTCTAGATAGCGCGATTGAAGAATATAAAAGCGGAATTATCGGTGTGATTATGACTGGGA
TGGGTAAAGACGGTTCTGCAGCGATCGTAAGAGTTAGAGAAATAGGTGGTTCTACGATTGCACAAGACGAACAAACTTCT
GTTATTTATGGGATGAATCGTCAAGCTGTGGAGATGGGAGGCGTCGAATATATTGAGCCTGTTACAGAAATCATCAATAG
GATCCAAATCATTTTGAAAGAGAGAGGAATTTAA

Upstream 100 bases:

>100_bases
TTACTGAATTCAATCACGAGGGTAACGATTTATTAGGTGTGTTATTATTCCTTCCGGATTTTGACGAACTGCTTCAATTG
ATTCCGAGGTTTTAATTTCG

Downstream 100 bases:

>100_bases
TATGGCCAGAATTCTCGTTGTGGATGACGCAAAATTTATGAGAACCATGGTAAAAGATGCACTGACCCAAACCGGTCACG
AAATCGTGGGAGAAGCAGAA

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIPNPVRAVIIDDSLLVRNIISDQIQKDSKIHVVATGKTGMDCIDLAQKMNPDVIILDVEMPVMDGLTALHELQKKKLGI
PVIMLSVLTQNGAEATFKALEYGAIDFVPKPSSVFQFDPEEIGNILKSKILAYFESKIQIPSQNLLKKAPAYSKVPAGSH
LKKSPIQAICIGTSTGGPRALQEVFSRIPADISLPILVVQHMPAGFTKAFATRLNDHAKIKVKEAEDGEPIEPNTGYVAP
GDAHLSIQSKGGRKWIALNREAPVNGHRPSIEVLLDSAIEEYKSGIIGVIMTGMGKDGSAAIVRVREIGGSTIAQDEQTS
VIYGMNRQAVEMGGVEYIEPVTEIINRIQIILKERGI

Sequences:

>Translated_357_residues
MIPNPVRAVIIDDSLLVRNIISDQIQKDSKIHVVATGKTGMDCIDLAQKMNPDVIILDVEMPVMDGLTALHELQKKKLGI
PVIMLSVLTQNGAEATFKALEYGAIDFVPKPSSVFQFDPEEIGNILKSKILAYFESKIQIPSQNLLKKAPAYSKVPAGSH
LKKSPIQAICIGTSTGGPRALQEVFSRIPADISLPILVVQHMPAGFTKAFATRLNDHAKIKVKEAEDGEPIEPNTGYVAP
GDAHLSIQSKGGRKWIALNREAPVNGHRPSIEVLLDSAIEEYKSGIIGVIMTGMGKDGSAAIVRVREIGGSTIAQDEQTS
VIYGMNRQAVEMGGVEYIEPVTEIINRIQIILKERGI
>Mature_357_residues
MIPNPVRAVIIDDSLLVRNIISDQIQKDSKIHVVATGKTGMDCIDLAQKMNPDVIILDVEMPVMDGLTALHELQKKKLGI
PVIMLSVLTQNGAEATFKALEYGAIDFVPKPSSVFQFDPEEIGNILKSKILAYFESKIQIPSQNLLKKAPAYSKVPAGSH
LKKSPIQAICIGTSTGGPRALQEVFSRIPADISLPILVVQHMPAGFTKAFATRLNDHAKIKVKEAEDGEPIEPNTGYVAP
GDAHLSIQSKGGRKWIALNREAPVNGHRPSIEVLLDSAIEEYKSGIIGVIMTGMGKDGSAAIVRVREIGGSTIAQDEQTS
VIYGMNRQAVEMGGVEYIEPVTEIINRIQIILKERGI

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=347, Percent_Identity=40.3458213256484, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1788550, Length=125, Percent_Identity=31.2, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_LEPIC (P62641)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002384.1
- ProteinModelPortal:   P62641
- SMR:   P62641
- GeneID:   2772514
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12455
- HOGENOM:   HBG705324
- OMA:   FMRKMIS
- ProtClustDB:   CLSK573833
- BioCyc:   LINT-130-01:LINT-130-01-002384-MONOMER
- BioCyc:   LINT267671:LIC_12455-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38713; Mature: 38713

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 174-174 ACT_SITE 201-201 ACT_SITE 297-297

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPNPVRAVIIDDSLLVRNIISDQIQKDSKIHVVATGKTGMDCIDLAQKMNPDVIILDVE
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEC
MPVMDGLTALHELQKKKLGIPVIMLSVLTQNGAEATFKALEYGAIDFVPKPSSVFQFDPE
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCEEECHH
EIGNILKSKILAYFESKIQIPSQNLLKKAPAYSKVPAGSHLKKSPIQAICIGTSTGGPRA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
LQEVFSRIPADISLPILVVQHMPAGFTKAFATRLNDHAKIKVKEAEDGEPIEPNTGYVAP
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECC
GDAHLSIQSKGGRKWIALNREAPVNGHRPSIEVLLDSAIEEYKSGIIGVIMTGMGKDGSA
CCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCE
AIVRVREIGGSTIAQDEQTSVIYGMNRQAVEMGGVEYIEPVTEIINRIQIILKERGI
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPNPVRAVIIDDSLLVRNIISDQIQKDSKIHVVATGKTGMDCIDLAQKMNPDVIILDVE
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEC
MPVMDGLTALHELQKKKLGIPVIMLSVLTQNGAEATFKALEYGAIDFVPKPSSVFQFDPE
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCEEECCCCCCCEEECHH
EIGNILKSKILAYFESKIQIPSQNLLKKAPAYSKVPAGSHLKKSPIQAICIGTSTGGPRA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
LQEVFSRIPADISLPILVVQHMPAGFTKAFATRLNDHAKIKVKEAEDGEPIEPNTGYVAP
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEECC
GDAHLSIQSKGGRKWIALNREAPVNGHRPSIEVLLDSAIEEYKSGIIGVIMTGMGKDGSA
CCCEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCE
AIVRVREIGGSTIAQDEQTSVIYGMNRQAVEMGGVEYIEPVTEIINRIQIILKERGI
EEEEEEECCCCCCCCCCCCEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA