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The map label for this gene is btuB

Identifier: 45658220

GI number: 45658220

Start: 2876376

End: 2878487

Strand: Reverse

Name: btuB

Synonym: LIC12374

Alternate gene names: 45658220

Gene position: 2878487-2876376 (Counterclockwise)

Preceding gene: 45658221

Following gene: 45658219

Centisome position: 67.3

GC content: 37.97

Gene sequence:

>2112_bases
ATGGGAAAAAATCAATACATTCAAATATTAAAATATTCTGTAATTCCATTTTTACTTGTGTTTGCTTTGACTTTAAGCGC
ACAGAATCAAGTTCCCGCTACAAAAGCCGAAACCGTTCAGGTAATTGGAAAAGTTCCAGACTCCAGTTCCCAAAATTTTC
GTTCCAATCCGAGCGGATTTCAAACTGCAATTAAACTAGACGAAACTTCGGCGCGTTATACGTCATTGCCGGAAGTTTTG
GAAAGAGAGGCTGGTCTTAGGGTTCGGTCTTTTGGAGGTTTGGGATCGTATTCCACTCTTTCTATTCGTGGCACAAATCC
AAATCAATCCAGAATCTATTTGGATGGTATTCCGCTAAACAATTCTCAAGGAGGAGAAGTAAATTTAGCGGATCTTCCTT
TTGATAGTTTAGAAAGTGTGGAAGTATATAGATCCGGAAATCCGATTGGTTTTTCGGGATCTGCGATCGGAGGTTCGGTC
AATCTGGTAACTAAAAAAGATTCCCAAAAACCAAAAACCAGGATCAATCTGGGAGGTGGTTCGTTTAATACTGGAAAGGC
GAGTGTCTCTCATACTGGAAACTACAAAGGAATTGGAACCAGTTTTTTAGCGTTAGGTGAAAAGTCTGATCAAAATTTTT
CCTACAAAAACGATCACGGAACGGTCGTTTTAAACACATTAGACGACACAATTGATAGAAGAAAAAACGCTTCTTTTGAA
AGGGCGGCGCTTTTTGGATCTTTAAAATACCAAATCGGAAAAACGGAACTAAAATTCTTAAACGATTTCAATCATAGGAT
CCACGGACTTCCAGGACCTGGCTCAAATCAAACGGATAGGGTTCATCGAAAATACGACAGAAATACCAGCTCTTTTGCTA
CAGACACCAAAGGGCTGTTTGTCGATTCCTTTCGATTGGAAACCAGAGGTTTTTATACTGCGGCTAAAGATGACCTTTAC
GATCCACTTTCAGAATTTTCTAAAGGAACCCCAAATTCCAGAGCAGAGATCAGACAAGCGGGTTTTCAGGTCATGCCTAC
TTTGTATTTAACTGATTATCATCAGATCTTGAGAGGATTTGTGTCTTTGGAAAAAGAGTCCTTTGATCGAGAGAGGTTAA
CTTCCTCTAACTTGGTAGGAAGGGTGGAGCCTAAAAAAGAAAGAACTTATTCTTCTTTTCGTCTGGAAGACGAGATCCGT
ATTTGGAACTCAAAAATACTTTTGATACCTTCCGTGACCTGGGATCATTATAAAGATCGTTTTCCTTCCGAAGAACCCTG
GTATAGAAGACAAGATCCACTTTCGGGTGATATCAAAAAAACTACATTCACAAATCCTAAATTAGGTTTTGTTTGGAAGA
TATTCGAAAAAGAAAGTTGGGATGTTCAGTTTCAAGCAAATATATCCAAACAGTATAGGATTCCTTCTTTTTTAGAAATG
TTTGGAGAACAGGGAAGTATCGTCGCAAATCCTAATTTGAAACCGGAACAAAGCGAAAACGGGGACGCAGGTTTTATATA
TAAAACGAATCATTCATTCCTAAAAACAAAATCGAGCATTTCTTATTTTAAAAAAGATATGAGAGATATGATTCTATTTC
TTCCAAACTCCCAGTTTACACTCAGGCCGGAAAACGTGGATTCCGCTAAAATTAGAGGATTAGAATTTTCTCATAGAGGA
GATTGGAAATACGGAATTAAGTTTTTATTCAACTATACGTATCAAGAAGCGATCAATACTTCTTCTTCTCCGTATTTACA
TGGAAAAATACTTCCTTTGAGACCCAGACACGAATTTTCCAGCACGTTTTCTTGGAAAGGAAAGAAACTGGAAACTGGAA
TAGAATTGTTGTATATCGGAGCCGTGTTCAGAGATAGAACCAACGAATACATCAATTATATACCGGAAAGACAGATCTGG
AACTATTTTTTCACTTGGGTAATCTACTCTGAATCAAAAGATTCCGATTTGAACGGAAATTCAAAGGAGATAACGAGAGA
GGTTCTGTTAACCTTTGAGGTCAAAAATTTTACAGACAAAAGGATATCCGATTTGATCGGTTATCCTCTACCTGGAAGAA
GCTGGTACACAACTCTGAGTATGAGATTTTAA

Upstream 100 bases:

>100_bases
ACTTTCGGGAGCTAAAGTCGGGTTTGAAAAATAGGCTTTTGTTTTTTAGATTTCTAATTGAAATCTCCGATTTTTCTTTC
CCATTTTATTCCGGCTAAAA

Downstream 100 bases:

>100_bases
AATGAGAACAAAACTTAAGGTTTTTATTTTATTCTTTTTTTTGAATGTATTTTGCAGAGACATTGAAAGGCCTTCATTTT
TAAGTCTGCTTCTGATTCAA

Product: outer membrane protein, TonB dependent

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MGKNQYIQILKYSVIPFLLVFALTLSAQNQVPATKAETVQVIGKVPDSSSQNFRSNPSGFQTAIKLDETSARYTSLPEVL
EREAGLRVRSFGGLGSYSTLSIRGTNPNQSRIYLDGIPLNNSQGGEVNLADLPFDSLESVEVYRSGNPIGFSGSAIGGSV
NLVTKKDSQKPKTRINLGGGSFNTGKASVSHTGNYKGIGTSFLALGEKSDQNFSYKNDHGTVVLNTLDDTIDRRKNASFE
RAALFGSLKYQIGKTELKFLNDFNHRIHGLPGPGSNQTDRVHRKYDRNTSSFATDTKGLFVDSFRLETRGFYTAAKDDLY
DPLSEFSKGTPNSRAEIRQAGFQVMPTLYLTDYHQILRGFVSLEKESFDRERLTSSNLVGRVEPKKERTYSSFRLEDEIR
IWNSKILLIPSVTWDHYKDRFPSEEPWYRRQDPLSGDIKKTTFTNPKLGFVWKIFEKESWDVQFQANISKQYRIPSFLEM
FGEQGSIVANPNLKPEQSENGDAGFIYKTNHSFLKTKSSISYFKKDMRDMILFLPNSQFTLRPENVDSAKIRGLEFSHRG
DWKYGIKFLFNYTYQEAINTSSSPYLHGKILPLRPRHEFSSTFSWKGKKLETGIELLYIGAVFRDRTNEYINYIPERQIW
NYFFTWVIYSESKDSDLNGNSKEITREVLLTFEVKNFTDKRISDLIGYPLPGRSWYTTLSMRF

Sequences:

>Translated_703_residues
MGKNQYIQILKYSVIPFLLVFALTLSAQNQVPATKAETVQVIGKVPDSSSQNFRSNPSGFQTAIKLDETSARYTSLPEVL
EREAGLRVRSFGGLGSYSTLSIRGTNPNQSRIYLDGIPLNNSQGGEVNLADLPFDSLESVEVYRSGNPIGFSGSAIGGSV
NLVTKKDSQKPKTRINLGGGSFNTGKASVSHTGNYKGIGTSFLALGEKSDQNFSYKNDHGTVVLNTLDDTIDRRKNASFE
RAALFGSLKYQIGKTELKFLNDFNHRIHGLPGPGSNQTDRVHRKYDRNTSSFATDTKGLFVDSFRLETRGFYTAAKDDLY
DPLSEFSKGTPNSRAEIRQAGFQVMPTLYLTDYHQILRGFVSLEKESFDRERLTSSNLVGRVEPKKERTYSSFRLEDEIR
IWNSKILLIPSVTWDHYKDRFPSEEPWYRRQDPLSGDIKKTTFTNPKLGFVWKIFEKESWDVQFQANISKQYRIPSFLEM
FGEQGSIVANPNLKPEQSENGDAGFIYKTNHSFLKTKSSISYFKKDMRDMILFLPNSQFTLRPENVDSAKIRGLEFSHRG
DWKYGIKFLFNYTYQEAINTSSSPYLHGKILPLRPRHEFSSTFSWKGKKLETGIELLYIGAVFRDRTNEYINYIPERQIW
NYFFTWVIYSESKDSDLNGNSKEITREVLLTFEVKNFTDKRISDLIGYPLPGRSWYTTLSMRF
>Mature_702_residues
GKNQYIQILKYSVIPFLLVFALTLSAQNQVPATKAETVQVIGKVPDSSSQNFRSNPSGFQTAIKLDETSARYTSLPEVLE
REAGLRVRSFGGLGSYSTLSIRGTNPNQSRIYLDGIPLNNSQGGEVNLADLPFDSLESVEVYRSGNPIGFSGSAIGGSVN
LVTKKDSQKPKTRINLGGGSFNTGKASVSHTGNYKGIGTSFLALGEKSDQNFSYKNDHGTVVLNTLDDTIDRRKNASFER
AALFGSLKYQIGKTELKFLNDFNHRIHGLPGPGSNQTDRVHRKYDRNTSSFATDTKGLFVDSFRLETRGFYTAAKDDLYD
PLSEFSKGTPNSRAEIRQAGFQVMPTLYLTDYHQILRGFVSLEKESFDRERLTSSNLVGRVEPKKERTYSSFRLEDEIRI
WNSKILLIPSVTWDHYKDRFPSEEPWYRRQDPLSGDIKKTTFTNPKLGFVWKIFEKESWDVQFQANISKQYRIPSFLEMF
GEQGSIVANPNLKPEQSENGDAGFIYKTNHSFLKTKSSISYFKKDMRDMILFLPNSQFTLRPENVDSAKIRGLEFSHRGD
WKYGIKFLFNYTYQEAINTSSSPYLHGKILPLRPRHEFSSTFSWKGKKLETGIELLYIGAVFRDRTNEYINYIPERQIWN
YFFTWVIYSESKDSDLNGNSKEITREVLLTFEVKNFTDKRISDLIGYPLPGRSWYTTLSMRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788478, Length=734, Percent_Identity=22.7520435967302, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1790405, Length=259, Percent_Identity=28.1853281853282, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 80205; Mature: 80074

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNQYIQILKYSVIPFLLVFALTLSAQNQVPATKAETVQVIGKVPDSSSQNFRSNPSGF
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCC
QTAIKLDETSARYTSLPEVLEREAGLRVRSFGGLGSYSTLSIRGTNPNQSRIYLDGIPLN
EEEEEECCCCCHHHCCHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEECC
NSQGGEVNLADLPFDSLESVEVYRSGNPIGFSGSAIGGSVNLVTKKDSQKPKTRINLGGG
CCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCC
SFNTGKASVSHTGNYKGIGTSFLALGEKSDQNFSYKNDHGTVVLNTLDDTIDRRKNASFE
CCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCEEEEECCHHHHHHHCCCCHH
RAALFGSLKYQIGKTELKFLNDFNHRIHGLPGPGSNQTDRVHRKYDRNTSSFATDTKGLF
HHHHHHHHHEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEE
VDSFRLETRGFYTAAKDDLYDPLSEFSKGTPNSRAEIRQAGFQVMPTLYLTDYHQILRGF
EEEEEEECCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHCCCEEECCHHHHHHHHHHHHH
VSLEKESFDRERLTSSNLVGRVEPKKERTYSSFRLEDEIRIWNSKILLIPSVTWDHYKDR
HHHHHHHCCHHHHHCCCCEECCCCHHHHCHHHEEECCEEEEECCEEEEEECCCHHHHHHC
FPSEEPWYRRQDPLSGDIKKTTFTNPKLGFVWKIFEKESWDVQFQANISKQYRIPSFLEM
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHH
FGEQGSIVANPNLKPEQSENGDAGFIYKTNHSFLKTKSSISYFKKDMRDMILFLPNSQFT
HCCCCCEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCEE
LRPENVDSAKIRGLEFSHRGDWKYGIKFLFNYTYQEAINTSSSPYLHGKILPLRPRHEFS
ECCCCCCCCEEECEEECCCCCHHEEEEEEEEEHHHHHHCCCCCCEEEEEEEECCCCHHCC
STFSWKGKKLETGIELLYIGAVFRDRTNEYINYIPERQIWNYFFTWVIYSESKDSDLNGN
CCCCCCCCCCCCCEEEEEEEHHHHHCHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCC
SKEITREVLLTFEVKNFTDKRISDLIGYPLPGRSWYTTLSMRF
HHHHHEEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
GKNQYIQILKYSVIPFLLVFALTLSAQNQVPATKAETVQVIGKVPDSSSQNFRSNPSGF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCC
QTAIKLDETSARYTSLPEVLEREAGLRVRSFGGLGSYSTLSIRGTNPNQSRIYLDGIPLN
EEEEEECCCCCHHHCCHHHHHHHCCCEEEECCCCCCCEEEEEECCCCCCCEEEEEEEECC
NSQGGEVNLADLPFDSLESVEVYRSGNPIGFSGSAIGGSVNLVTKKDSQKPKTRINLGGG
CCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCC
SFNTGKASVSHTGNYKGIGTSFLALGEKSDQNFSYKNDHGTVVLNTLDDTIDRRKNASFE
CCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCEEEEECCHHHHHHHCCCCHH
RAALFGSLKYQIGKTELKFLNDFNHRIHGLPGPGSNQTDRVHRKYDRNTSSFATDTKGLF
HHHHHHHHHEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCEE
VDSFRLETRGFYTAAKDDLYDPLSEFSKGTPNSRAEIRQAGFQVMPTLYLTDYHQILRGF
EEEEEEECCCEEEECHHHHHHHHHHHHCCCCCCHHHHHHCCCEEECCHHHHHHHHHHHHH
VSLEKESFDRERLTSSNLVGRVEPKKERTYSSFRLEDEIRIWNSKILLIPSVTWDHYKDR
HHHHHHHCCHHHHHCCCCEECCCCHHHHCHHHEEECCEEEEECCEEEEEECCCHHHHHHC
FPSEEPWYRRQDPLSGDIKKTTFTNPKLGFVWKIFEKESWDVQFQANISKQYRIPSFLEM
CCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCEEEEECCCCCCCCHHHHHH
FGEQGSIVANPNLKPEQSENGDAGFIYKTNHSFLKTKSSISYFKKDMRDMILFLPNSQFT
HCCCCCEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEECCCCEE
LRPENVDSAKIRGLEFSHRGDWKYGIKFLFNYTYQEAINTSSSPYLHGKILPLRPRHEFS
ECCCCCCCCEEECEEECCCCCHHEEEEEEEEEHHHHHHCCCCCCEEEEEEEECCCCHHCC
STFSWKGKKLETGIELLYIGAVFRDRTNEYINYIPERQIWNYFFTWVIYSESKDSDLNGN
CCCCCCCCCCCCCEEEEEEEHHHHHCHHHHHHCCCCHHHHEEEEEEEEEECCCCCCCCCC
SKEITREVLLTFEVKNFTDKRISDLIGYPLPGRSWYTTLSMRF
HHHHHEEEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA