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The map label for this gene is glyA

Identifier: 45658181

GI number: 45658181

Start: 2819403

End: 2820650

Strand: Direct

Name: glyA

Synonym: LIC12335

Alternate gene names: 45658181

Gene position: 2819403-2820650 (Clockwise)

Preceding gene: 45658180

Following gene: 45658182

Centisome position: 65.92

GC content: 41.59

Gene sequence:

>1248_bases
ATGCAGTTTCTTCCAAAAGCAGATCCAGAAATATTTGCAGCATTAAAAAAAGAGGATGAAAGACAGGAAAATAACCTGGA
AATGATCGCCTCTGAAAATTTTGTTTCCAGAGCCGTTCTGGAAGCTTATACATCCACACTTACGAATAAATATGCCGAAG
GTTATCCAGGCAAAAGATATTACAACGGTTGTCATAACGCTGACGTAGTGGAAACACTCGCTATCGAAAGAGCAAAAAAA
CTTTTTGGTTCTCAATACGCAAACGTTCAACCTCACTCCGGCGCTCAGGCAAATATGGCCGTTTTTCTCGCTTGTTTAGA
ACCTGGAGATTCTTTTTTAGGAATGAATCTGGCTCACGGAGGTCATTTAACTCACGGTTCTCCGGTCAATGTAAGCGGAA
AAATTTATAAGCCGATTCCTTATGGAGTGGATTCTAAAACCGAAACCATCAACTACGACGAAGTCGCAAAACTCGCGAGA
GAACATAAACCAAAGTTGATTGTTGCAGGAGCTTCCGCTTATGCAAGAACGATCGACTTTTCTAAGTTTGCTGAGATTGC
AAAGGAAGTAGGTGCAAAATTGATGGCGGATATTGCACATATTTCAGGTCTTGTTGCTACTGGTTATCATCCTTCTCCAA
TTGGTATGTTTGATTTTGTGACCACCACCACTCACAAAACTTTGAGAGGTCCCAGAGGTGGGTTGATTCTTTCTACATTA
GAAAATGAGAAAGTACTAAACTCTCGTGTTTTTCCAGGAATCCAAGGTGGACCTTTGATGCACGTGATCGCGGCCAAAGC
GGTGGCATTTCAGGAAGCACTTCAGCCAGACTATAAAAAATATATAGAAACCGTTCTTGCAAACGCCAAAACTTTAGCGG
AAGTTTTTGTAAAAAGAGGTTACAGAGTTGTAAGCGGTGGAACGGACAATCACCTAGTTCTTTTGGACGTTTCCGTAAAA
GGACTTACCGGTGCACAAGCAGCTGATGGACTAGACGAGGTAGGAGTTACCGTAAATAAAAACGCGATCCCGTTTGATAA
AAATCCTCCCGCTGTCGCTTCTGGAATTCGTCTAGGAACTCCCGCACTTACAACCCGAGGTCTAAAACCTGCAGATATGG
AAACTGTGGGAAATTTAATCTGCGACTTTTTAGATCATCCAAACGATGATAAAAATAGAACAAAAGTCAAAGGTGGCATC
CAAGAGATGACTCAGAAGTTTCCTATGAATCAATTTCGTTTAGATTGA

Upstream 100 bases:

>100_bases
AGAAGCAAAATTGCTTTTCGAAAACAAATATTCTAGGATGGATTTTATCTTCAGAGACTAAATCGGACTTTTACTCGGAA
CTATCAGAAAGAAGGTCCAG

Downstream 100 bases:

>100_bases
TCGAACGATTTCTTTAGAACGAAGAGCGTGTCCCAAAACCTAAACACCGACCCTTAGGAAGGTGTTTGCTGAGTTTCACA
AAATGTGGGAACTCATACAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADVVETLAIERAKK
LFGSQYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGKIYKPIPYGVDSKTETINYDEVAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVATGYHPSPIGMFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFQEALQPDYKKYIETVLANAKTLAEVFVKRGYRVVSGGTDNHLVLLDVSVK
GLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDHPNDDKNRTKVKGGI
QEMTQKFPMNQFRLD

Sequences:

>Translated_415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADVVETLAIERAKK
LFGSQYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGKIYKPIPYGVDSKTETINYDEVAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVATGYHPSPIGMFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFQEALQPDYKKYIETVLANAKTLAEVFVKRGYRVVSGGTDNHLVLLDVSVK
GLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDHPNDDKNRTKVKGGI
QEMTQKFPMNQFRLD
>Mature_415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADVVETLAIERAKK
LFGSQYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGKIYKPIPYGVDSKTETINYDEVAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVATGYHPSPIGMFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFQEALQPDYKKYIETVLANAKTLAEVFVKRGYRVVSGGTDNHLVLLDVSVK
GLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDHPNDDKNRTKVKGGI
QEMTQKFPMNQFRLD

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=408, Percent_Identity=46.5686274509804, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI261862352, Length=408, Percent_Identity=46.5686274509804, Blast_Score=350, Evalue=9e-97,
Organism=Homo sapiens, GI261862350, Length=408, Percent_Identity=46.5686274509804, Blast_Score=350, Evalue=9e-97,
Organism=Homo sapiens, GI261862348, Length=408, Percent_Identity=46.5686274509804, Blast_Score=350, Evalue=9e-97,
Organism=Homo sapiens, GI261862346, Length=408, Percent_Identity=45.5882352941176, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI22547186, Length=436, Percent_Identity=42.8899082568807, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI22547189, Length=421, Percent_Identity=40.855106888361, Blast_Score=292, Evalue=4e-79,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=56.5432098765432, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI25144732, Length=398, Percent_Identity=47.2361809045226, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=398, Percent_Identity=47.2361809045226, Blast_Score=345, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=399, Percent_Identity=44.6115288220551, Blast_Score=338, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=437, Percent_Identity=41.1899313501144, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24640005, Length=403, Percent_Identity=48.6352357320099, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=403, Percent_Identity=48.6352357320099, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 44975; Mature: 44975

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRY
CCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCHNADVVETLAIERAKKLFGSQYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHG
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCC
GHLTHGSPVNVSGKIYKPIPYGVDSKTETINYDEVAKLAREHKPKLIVAGASAYARTIDF
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCH
SKFAEIAKEVGAKLMADIAHISGLVATGYHPSPIGMFDFVTTTTHKTLRGPRGGLILSTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFQEALQPDYKKYIETVLANAKTLAEVFVKRG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
YRVVSGGTDNHLVLLDVSVKGLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCHHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKPADMETVGNLICDFLDHPNDDKNRTKVKGGIQEMTQKFPMNQFRLD
CHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRY
CCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCHNADVVETLAIERAKKLFGSQYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHG
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCC
GHLTHGSPVNVSGKIYKPIPYGVDSKTETINYDEVAKLAREHKPKLIVAGASAYARTIDF
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCH
SKFAEIAKEVGAKLMADIAHISGLVATGYHPSPIGMFDFVTTTTHKTLRGPRGGLILSTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEC
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFQEALQPDYKKYIETVLANAKTLAEVFVKRG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
YRVVSGGTDNHLVLLDVSVKGLTGAQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCHHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKPADMETVGNLICDFLDHPNDDKNRTKVKGGIQEMTQKFPMNQFRLD
CHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA