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The map label for this gene is prpB [C]

Identifier: 45658177

GI number: 45658177

Start: 2813956

End: 2814801

Strand: Reverse

Name: prpB [C]

Synonym: LIC12331

Alternate gene names: 45658177

Gene position: 2814801-2813956 (Counterclockwise)

Preceding gene: 45658178

Following gene: 45658176

Centisome position: 65.81

GC content: 42.32

Gene sequence:

>846_bases
ATGTCTATATCACAAAGTAAAAAAGCCGAAATGTTCCGTAGTTTGCACGAGCGGGGTACTTTCATTATGCCAAATGCCTG
GGATGCAGGAAGCGCGCGTATGTTGGTTGGTTCCGGATTCTCGGCGATTGGAACTACCAGCGCGGGAATTGCTTTTGCTG
CTGGATTGCCAGATCATCAGATTCTAGATCGTGACGTTATGCTCGAATATGTGAGAAATATAGTAGCATCGGTTGATATT
CCAGTAAGTGCTGATCTAGAAAGTGGGTATGGAATAGAACCAGATAAAGTTGCTGAAACTGTTAGGCGGGCTATATCGAT
AGGAGTAGTGGGTTGTAATATTGAAGATCTGAGTGGCGATAATTCTTCACCTCTGCTGAATGCTGAGTTAGCTGCCGAAC
GTATTCACTTTGCTCGTCAAGAAGCAAACGCAGCCGGTTTCGCATTTACTCTAACTGCTAGAACTGATACGTTTCTTACG
AATCATCCCAACGCTTTAGACGAAACGATTCGTCGAGCCGATATGTATCGTAAAGCAGGCGCGGATTGTCTTTTTATTCC
GGGAGTCAATGATATTGAAACGATTGGTAATTTAGTTCGTTCCATCGACTGCCCGCTTAATGTTGTTGTAGGACTCGGTA
AAAATAATTTCACCGCAGCCGATTTACTGTCTTTAGGTGTAAGACGGATCAGTATTGGAGGAAGTCTTGCCCGTGCTTGT
TTTTATTTGATTCATCAGGCTGCGCTTGAAATGTTTAACCGAGGTACTTTTAATTTTACAGAAACACAACTTACTCATAA
AGAACTTTGTGATTTTTTTGCAGCATTCGAGACAAAAGAAAAATGA

Upstream 100 bases:

>100_bases
TTTTGCTTGGTGAAATCCTCTGTTAAGATTTTAATATTAAAAATAAATCATATGAAATTATCTGTTGACTGTTAAACTGA
GTTGAGGTATTGAAGGCCTA

Downstream 100 bases:

>100_bases
AATCTTTTGTTGTAATAACCTAGTTACGTAAAAACGGACACCTTTTACTCAGCGAATGAGGTAAAGGTGAATATGAATAA
GAAAGGAAAATACTCTCCGG

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSISQSKKAEMFRSLHERGTFIMPNAWDAGSARMLVGSGFSAIGTTSAGIAFAAGLPDHQILDRDVMLEYVRNIVASVDI
PVSADLESGYGIEPDKVAETVRRAISIGVVGCNIEDLSGDNSSPLLNAELAAERIHFARQEANAAGFAFTLTARTDTFLT
NHPNALDETIRRADMYRKAGADCLFIPGVNDIETIGNLVRSIDCPLNVVVGLGKNNFTAADLLSLGVRRISIGGSLARAC
FYLIHQAALEMFNRGTFNFTETQLTHKELCDFFAAFETKEK

Sequences:

>Translated_281_residues
MSISQSKKAEMFRSLHERGTFIMPNAWDAGSARMLVGSGFSAIGTTSAGIAFAAGLPDHQILDRDVMLEYVRNIVASVDI
PVSADLESGYGIEPDKVAETVRRAISIGVVGCNIEDLSGDNSSPLLNAELAAERIHFARQEANAAGFAFTLTARTDTFLT
NHPNALDETIRRADMYRKAGADCLFIPGVNDIETIGNLVRSIDCPLNVVVGLGKNNFTAADLLSLGVRRISIGGSLARAC
FYLIHQAALEMFNRGTFNFTETQLTHKELCDFFAAFETKEK
>Mature_280_residues
SISQSKKAEMFRSLHERGTFIMPNAWDAGSARMLVGSGFSAIGTTSAGIAFAAGLPDHQILDRDVMLEYVRNIVASVDIP
VSADLESGYGIEPDKVAETVRRAISIGVVGCNIEDLSGDNSSPLLNAELAAERIHFARQEANAAGFAFTLTARTDTFLTN
HPNALDETIRRADMYRKAGADCLFIPGVNDIETIGNLVRSIDCPLNVVVGLGKNNFTAADLLSLGVRRISIGGSLARACF
YLIHQAALEMFNRGTFNFTETQLTHKELCDFFAAFETKEK

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=296, Percent_Identity=26.0135135135135, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 30457; Mature: 30326

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISQSKKAEMFRSLHERGTFIMPNAWDAGSARMLVGSGFSAIGTTSAGIAFAAGLPDHQ
CCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCHHHCCCCCCEEEECCCCCHH
ILDRDVMLEYVRNIVASVDIPVSADLESGYGIEPDKVAETVRRAISIGVVGCNIEDLSGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCHHCCCC
NSSPLLNAELAAERIHFARQEANAAGFAFTLTARTDTFLTNHPNALDETIRRADMYRKAG
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCC
ADCLFIPGVNDIETIGNLVRSIDCPLNVVVGLGKNNFTAADLLSLGVRRISIGGSLARAC
CCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHEECCHHHHHHH
FYLIHQAALEMFNRGTFNFTETQLTHKELCDFFAAFETKEK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SISQSKKAEMFRSLHERGTFIMPNAWDAGSARMLVGSGFSAIGTTSAGIAFAAGLPDHQ
CCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCHHHCCCCCCEEEECCCCCHH
ILDRDVMLEYVRNIVASVDIPVSADLESGYGIEPDKVAETVRRAISIGVVGCNIEDLSGD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCHHCCCC
NSSPLLNAELAAERIHFARQEANAAGFAFTLTARTDTFLTNHPNALDETIRRADMYRKAG
CCCCEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHHHHHCC
ADCLFIPGVNDIETIGNLVRSIDCPLNVVVGLGKNNFTAADLLSLGVRRISIGGSLARAC
CCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHEECCHHHHHHH
FYLIHQAALEMFNRGTFNFTETQLTHKELCDFFAAFETKEK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]