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The map label for this gene is hpcH [H]

Identifier: 45658007

GI number: 45658007

Start: 2608630

End: 2609385

Strand: Reverse

Name: hpcH [H]

Synonym: LIC12158

Alternate gene names: 45658007

Gene position: 2609385-2608630 (Counterclockwise)

Preceding gene: 45658008

Following gene: 45658006

Centisome position: 61.01

GC content: 40.48

Gene sequence:

>756_bases
ATGAACTTCGTAAAAGAGAAACTAAAGTCCGGAAAGTTTACGGTTGGATCCTGGATGCAAATTCCAAATACTTCGATCGC
GGAAATTTTAGGAAGCGCCGGTTTTGATTGGATTGCTTTGGATTTAGAACACGGTGCTTTTAGTCTAAATCTTCTCCCGG
ATCTTTTTAGAGCCATCAGTAAGAACGGAGCTTTACCTTTTGCAAGGGTAGCTCAAAACCACCCGAAAGATATTAAACAA
GCTTTAGACGCAGGGGCTAGAGGGATTATCGTTCCTATGATCGAAACTGCTGAACAGGTGCAGGGTTTTCTAAAATGGAG
TAGTTATCCGCCGGATGGAACAAGAGGAGTGGGTTATTCGAATGCGAATTTGTTCGGAAAATACTTTGACTCTTATTCAA
AAGAGATCAATTCGAACTTAATACGCGTCATTCAAATCGAAAATATCAAAGCGATTCAACAAATTGATTCGATACTTTCC
GTAAAAGGAATCGATGTCATGATGATCGGTCCCTACGATTTGTCCGGTTCAATGGGATTGACCGGCCAATTTGAACATCC
TGATTTTTTACACGCAATTGGAGAACTTCATAAGGCAGCGGAACGGAATGGAATTCCTCTTGGCACGCATGTGATCCAGG
CGGACTTTCAAAAAGTAAAAGATGAAATTCGTACCGGTAAGAAGTTTGTCGCCTATTCTACGGATGCGATTATGTTAAAT
ACGCAAGCTAGTTTGAATTTTGAAGAATTAAAATAG

Upstream 100 bases:

>100_bases
ATAAGGTAAAAAGTTTTTCAAAATCACTTCAACAAGAATCGATCGAAGTCATCCACCATTTGCTCTATGAGTTTGCGGTT
TTATTAGAAAAATAATATAT

Downstream 100 bases:

>100_bases
TTTAAAATTATTAATTACTAATTTTGGAATAAATATGAAAACGATTGCTGTTTTGCCGGCGAGAATGGCCTCTTCCAGGT
TTCCCGATAAACCGTTGGTC

Product: putative hydroxyacid aldolase protein

Products: pyruvate; tartronate semialdehyde

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNFVKEKLKSGKFTVGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFSLNLLPDLFRAISKNGALPFARVAQNHPKDIKQ
ALDAGARGIIVPMIETAEQVQGFLKWSSYPPDGTRGVGYSNANLFGKYFDSYSKEINSNLIRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFEHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRTGKKFVAYSTDAIMLN
TQASLNFEELK

Sequences:

>Translated_251_residues
MNFVKEKLKSGKFTVGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFSLNLLPDLFRAISKNGALPFARVAQNHPKDIKQ
ALDAGARGIIVPMIETAEQVQGFLKWSSYPPDGTRGVGYSNANLFGKYFDSYSKEINSNLIRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFEHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRTGKKFVAYSTDAIMLN
TQASLNFEELK
>Mature_251_residues
MNFVKEKLKSGKFTVGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFSLNLLPDLFRAISKNGALPFARVAQNHPKDIKQ
ALDAGARGIIVPMIETAEQVQGFLKWSSYPPDGTRGVGYSNANLFGKYFDSYSKEINSNLIRVIQIENIKAIQQIDSILS
VKGIDVMMIGPYDLSGSMGLTGQFEHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRTGKKFVAYSTDAIMLN
TQASLNFEELK

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=236, Percent_Identity=32.6271186440678, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1788578, Length=239, Percent_Identity=31.7991631799163, Blast_Score=135, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20

Molecular weight: Translated: 27660; Mature: 27660

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVKEKLKSGKFTVGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFSLNLLPDLFRAIS
CCHHHHHHHCCCEEECCEEECCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHH
KNGALPFARVAQNHPKDIKQALDAGARGIIVPMIETAEQVQGFLKWSSYPPDGTRGVGYS
CCCCCCHHHHHCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NANLFGKYFDSYSKEINSNLIRVIQIENIKAIQQIDSILSVKGIDVMMIGPYDLSGSMGL
CCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
TGQFEHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRTGKKFVAYSTDAIMLN
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
TQASLNFEELK
CCCCCCHHHCC
>Mature Secondary Structure
MNFVKEKLKSGKFTVGSWMQIPNTSIAEILGSAGFDWIALDLEHGAFSLNLLPDLFRAIS
CCHHHHHHHCCCEEECCEEECCCCHHHHHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHH
KNGALPFARVAQNHPKDIKQALDAGARGIIVPMIETAEQVQGFLKWSSYPPDGTRGVGYS
CCCCCCHHHHHCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NANLFGKYFDSYSKEINSNLIRVIQIENIKAIQQIDSILSVKGIDVMMIGPYDLSGSMGL
CCHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
TGQFEHPDFLHAIGELHKAAERNGIPLGTHVIQADFQKVKDEIRTGKKFVAYSTDAIMLN
CCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEE
TQASLNFEELK
CCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-dehydro-3-deoxy-D-glucarate

Specific reaction: 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde

General reaction: Carbon-Carbon Lyases; Aldehyde-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA