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The map label for this gene is lipB

Identifier: 45657920

GI number: 45657920

Start: 2510737

End: 2511387

Strand: Reverse

Name: lipB

Synonym: LIC12072

Alternate gene names: 45657920

Gene position: 2511387-2510737 (Counterclockwise)

Preceding gene: 45657924

Following gene: 45657919

Centisome position: 58.72

GC content: 35.94

Gene sequence:

>651_bases
GTGAAAATAATTGAATTTCGAAAGAAAATTCCTTACCTCCGTTACTTGGAAATGCAGGAAAAACTGCGAAAGTTTCGGAA
GGAATGTATTCTATTTTTGGAACACGCTCCTACAATCACCGGGGGAATCAATTATAATCCAGAGAATCTTTTAGTTAAGC
CAGAATTTCTGGAATCGATGGGAATTCAAATCCATTGGACCCAAAGAGGGGGAGATTTTACCGCTCATGAGCCGGGACAA
CTCGTTTTGTATTCTCATGTGGATTTAAAAAAGAGAAATCTTTCTATTCGTTTTTATTTAGAAAATTTACTTCGGTCTGT
TATCGATTCGGTCAGATCCACTTGGGATTTGCAGTTGATTTCCGATTCTGATTCTCCTGGTTTATATTTGGAATCTAATC
CCTCTCAAAAGATCTGTTCGATCGGAGTGAATTTTAAATCCTTTTTTACCAGTCACGGAATTGCATTCAATTTAAGTAAT
GATTTGAAAACGTTTCGTTGTATCAATCCCTGCGGCCGAAATTGGACGAATATGACGAGTGTAAAAGATTTAGGTCTTGA
TTTTGGTCTTCATAAAAGAGACGAATTGATTTCTTGTTTGAAAAAGAATTTATGTTCTTTCTTAGAACCGATAAATGTGA
GTTCGTCTTAA

Upstream 100 bases:

>100_bases
AGTAAATCAGTTTAGAGAGTGATAAACGATCTCTTCCGTTCGGAGATTTTTGTAGGATAAAAGAGATGACTTCTAAGAGT
TTTTCCATATAAAGAATCTT

Downstream 100 bases:

>100_bases
ACTTTGAAACTTGTGGGAACTCTTACAAATTGGGATTTTGCGGATAAACTTTGAAACTTGTGGGAACTCTTACAAATTGG
GATTTTGCGGATAAACTTTG

Product: lipoate protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKIIEFRKKIPYLRYLEMQEKLRKFRKECILFLEHAPTITGGINYNPENLLVKPEFLESMGIQIHWTQRGGDFTAHEPGQ
LVLYSHVDLKKRNLSIRFYLENLLRSVIDSVRSTWDLQLISDSDSPGLYLESNPSQKICSIGVNFKSFFTSHGIAFNLSN
DLKTFRCINPCGRNWTNMTSVKDLGLDFGLHKRDELISCLKKNLCSFLEPINVSSS

Sequences:

>Translated_216_residues
MKIIEFRKKIPYLRYLEMQEKLRKFRKECILFLEHAPTITGGINYNPENLLVKPEFLESMGIQIHWTQRGGDFTAHEPGQ
LVLYSHVDLKKRNLSIRFYLENLLRSVIDSVRSTWDLQLISDSDSPGLYLESNPSQKICSIGVNFKSFFTSHGIAFNLSN
DLKTFRCINPCGRNWTNMTSVKDLGLDFGLHKRDELISCLKKNLCSFLEPINVSSS
>Mature_216_residues
MKIIEFRKKIPYLRYLEMQEKLRKFRKECILFLEHAPTITGGINYNPENLLVKPEFLESMGIQIHWTQRGGDFTAHEPGQ
LVLYSHVDLKKRNLSIRFYLENLLRSVIDSVRSTWDLQLISDSDSPGLYLESNPSQKICSIGVNFKSFFTSHGIAFNLSN
DLKTFRCINPCGRNWTNMTSVKDLGLDFGLHKRDELISCLKKNLCSFLEPINVSSS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=194, Percent_Identity=30.9278350515464, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI87081767, Length=152, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323268, Length=195, Percent_Identity=27.1794871794872, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355483, Length=183, Percent_Identity=31.6939890710383, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_LEPIC (Q72QP1)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_002006.1
- ProteinModelPortal:   Q72QP1
- SMR:   Q72QP1
- GeneID:   2771759
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12072
- NMPDR:   fig|267671.1.peg.2006
- HOGENOM:   HBG716160
- OMA:   RDELISC
- ProtClustDB:   CLSK574038
- BioCyc:   LINT267671:LIC_12072-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24952; Mature: 24952

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIEFRKKIPYLRYLEMQEKLRKFRKECILFLEHAPTITGGINYNPENLLVKPEFLESM
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEECHHHHHHC
GIQIHWTQRGGDFTAHEPGQLVLYSHVDLKKRNLSIRFYLENLLRSVIDSVRSTWDLQLI
CCEEEEECCCCCCCCCCCCCEEEEEECCEEHHCCEEHHHHHHHHHHHHHHHHCCEEEEEE
SDSDSPGLYLESNPSQKICSIGVNFKSFFTSHGIAFNLSNDLKTFRCINPCGRNWTNMTS
ECCCCCCEEEECCCCCCCEEECCCHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCCCCH
VKDLGLDFGLHKRDELISCLKKNLCSFLEPINVSSS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIIEFRKKIPYLRYLEMQEKLRKFRKECILFLEHAPTITGGINYNPENLLVKPEFLESM
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCEEECHHHHHHC
GIQIHWTQRGGDFTAHEPGQLVLYSHVDLKKRNLSIRFYLENLLRSVIDSVRSTWDLQLI
CCEEEEECCCCCCCCCCCCCEEEEEECCEEHHCCEEHHHHHHHHHHHHHHHHCCEEEEEE
SDSDSPGLYLESNPSQKICSIGVNFKSFFTSHGIAFNLSNDLKTFRCINPCGRNWTNMTS
ECCCCCCEEEECCCCCCCEEECCCHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCCCCCH
VKDLGLDFGLHKRDELISCLKKNLCSFLEPINVSSS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA