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The map label for this gene is folD

Identifier: 45657878

GI number: 45657878

Start: 2443536

End: 2444390

Strand: Direct

Name: folD

Synonym: LIC12026

Alternate gene names: 45657878

Gene position: 2443536-2444390 (Clockwise)

Preceding gene: 45657873

Following gene: 45657879

Centisome position: 57.13

GC content: 40.23

Gene sequence:

>855_bases
ATGAATCCAGTTCTTTTAGATGGAAAAAAACTCTCCGAAAAAATTAAGGAAGAAATTCGTTCTGCAATTGAAGAACGAAA
AACTAAAAATTTTAGAATTCCAAAACTGGCCACAATTTTAGTGGGAAACAATCCAGCTTCCGAAACCTACGTTTCTATGA
AAGTCAAAGCCTGTCATTCTGTAGGAATGGGTTCGGAGATGATCCGTCTTAGAGAACAAACCACCACCAAAGAGTTGTTA
GACGTTATAGATAAACTCAACTCAGATCCCAACGTGGACGGAATTCTTCTTCAACATCCAACTCCTTCCGGAATCGACGA
AAGGGCTGCCTTTGATCGGATCGCTCTTCATAAGGACGTGGATGGAGTCACTACTCTTTCTTTCGGAAAACTTTCTATGG
GAGTGGAAACCTATCTACCTTGTACTCCGTACGGTATGGTTCTTTTATTGAAAGAATATGGAATTGATGTAGCAGGAAAA
AATGCAGTAGTAGTAGGTCGTTCTCCTATTTTAGGAAAACCAATGGCGATGCTTCTTACTGAAATGAATGCAACTGTCAC
ACTCTGTCATTCTAAAACCCAGAATCTCCCTGACATCGTTCGTAACGCAGATATTATCATAGGCGCAGTAGGCAAACCTG
AATTTATCAAGGCGGATTGGATTTCTAACGGCGCCATTCTTTTAGACGCAGGTTACAATCCAGGCAATATAGGAGACATT
GAAATTTCTAAAGCAAAGGATCGTTCTTCTTTTTATACTCCTGTTCCGGGTGGAGTTGGTCCGATGACAATTGCAGTACT
TTTATTACAGACTCTTTATTCAGCAAAAGAACACTTTACACCACCGGTAAAGTGA

Upstream 100 bases:

>100_bases
CAATTGGAGTTTCAGACATGAACCCATTTTACGGTTTTGATTCCACCGGAAAGGATATTTTTTACCTGACAGAGAAGACA
GTCTCTAAATCCTGAGAAGT

Downstream 100 bases:

>100_bases
AAACGATGTTTCCAGATGGCGATCAGTTTTGACGAATGTTTTCAAACCTATCCGGAGCTTCATAGTCCGAGTGATCTAGA
AGAATTTTGGTCCGAGGCAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNPVLLDGKKLSEKIKEEIRSAIEERKTKNFRIPKLATILVGNNPASETYVSMKVKACHSVGMGSEMIRLREQTTTKELL
DVIDKLNSDPNVDGILLQHPTPSGIDERAAFDRIALHKDVDGVTTLSFGKLSMGVETYLPCTPYGMVLLLKEYGIDVAGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPDIVRNADIIIGAVGKPEFIKADWISNGAILLDAGYNPGNIGDI
EISKAKDRSSFYTPVPGGVGPMTIAVLLLQTLYSAKEHFTPPVK

Sequences:

>Translated_284_residues
MNPVLLDGKKLSEKIKEEIRSAIEERKTKNFRIPKLATILVGNNPASETYVSMKVKACHSVGMGSEMIRLREQTTTKELL
DVIDKLNSDPNVDGILLQHPTPSGIDERAAFDRIALHKDVDGVTTLSFGKLSMGVETYLPCTPYGMVLLLKEYGIDVAGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPDIVRNADIIIGAVGKPEFIKADWISNGAILLDAGYNPGNIGDI
EISKAKDRSSFYTPVPGGVGPMTIAVLLLQTLYSAKEHFTPPVK
>Mature_284_residues
MNPVLLDGKKLSEKIKEEIRSAIEERKTKNFRIPKLATILVGNNPASETYVSMKVKACHSVGMGSEMIRLREQTTTKELL
DVIDKLNSDPNVDGILLQHPTPSGIDERAAFDRIALHKDVDGVTTLSFGKLSMGVETYLPCTPYGMVLLLKEYGIDVAGK
NAVVVGRSPILGKPMAMLLTEMNATVTLCHSKTQNLPDIVRNADIIIGAVGKPEFIKADWISNGAILLDAGYNPGNIGDI
EISKAKDRSSFYTPVPGGVGPMTIAVLLLQTLYSAKEHFTPPVK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=296, Percent_Identity=42.5675675675676, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=40.2730375426621, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=40.6143344709898, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI36796743, Length=226, Percent_Identity=26.9911504424779, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=45.2631578947368, Blast_Score=248, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=38.7543252595156, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319558, Length=289, Percent_Identity=41.8685121107266, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=38.3838383838384, Blast_Score=204, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6322933, Length=308, Percent_Identity=25.6493506493506, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=39.4557823129252, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=39.4557823129252, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=40.2777777777778, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=40.2777777777778, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=40.2777777777778, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=40.2777777777778, Blast_Score=212, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_LEPIC (Q72QT3)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001964.1
- HSSP:   P11586
- ProteinModelPortal:   Q72QT3
- SMR:   Q72QT3
- GeneID:   2771905
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC12026
- HOGENOM:   HBG328751
- OMA:   GPMTINT
- ProtClustDB:   PRK14177
- BioCyc:   LINT267671:LIC_12026-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30827; Mature: 30827

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVLLDGKKLSEKIKEEIRSAIEERKTKNFRIPKLATILVGNNPASETYVSMKVKACHS
CCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHH
VGMGSEMIRLREQTTTKELLDVIDKLNSDPNVDGILLQHPTPSGIDERAAFDRIALHKDV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
DGVTTLSFGKLSMGVETYLPCTPYGMVLLLKEYGIDVAGKNAVVVGRSPILGKPMAMLLT
CCCEEEECCHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHH
EMNATVTLCHSKTQNLPDIVRNADIIIGAVGKPEFIKADWISNGAILLDAGYNPGNIGDI
HCCCEEEEECCCCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCE
EISKAKDRSSFYTPVPGGVGPMTIAVLLLQTLYSAKEHFTPPVK
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPVLLDGKKLSEKIKEEIRSAIEERKTKNFRIPKLATILVGNNPASETYVSMKVKACHS
CCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHH
VGMGSEMIRLREQTTTKELLDVIDKLNSDPNVDGILLQHPTPSGIDERAAFDRIALHKDV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCC
DGVTTLSFGKLSMGVETYLPCTPYGMVLLLKEYGIDVAGKNAVVVGRSPILGKPMAMLLT
CCCEEEECCHHHCCHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHH
EMNATVTLCHSKTQNLPDIVRNADIIIGAVGKPEFIKADWISNGAILLDAGYNPGNIGDI
HCCCEEEEECCCCCCCHHHHCCCCEEEECCCCCCEEEEEECCCCEEEEECCCCCCCCCCE
EISKAKDRSSFYTPVPGGVGPMTIAVLLLQTLYSAKEHFTPPVK
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA