Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 45657715

GI number: 45657715

Start: 2246768

End: 2248072

Strand: Direct

Name: mdeA [H]

Synonym: LIC11852

Alternate gene names: 45657715

Gene position: 2246768-2248072 (Clockwise)

Preceding gene: 45657713

Following gene: 45657716

Centisome position: 52.53

GC content: 39.92

Gene sequence:

>1305_bases
ATGCCTAGAAATTATAAGCCAGAAACAATTGCGCTGCACGGAGGTCAATCTCCAGACCCTTCTACTTTATCTAGGGCAGT
TCCAATTTATCAAACTACATCTTATGTGTTTAAAAACACTGAACACGCCGCTAAACTTTTTGGCCTACAGGAATTCGGAA
ATATCTATACAAGGATTATGAATCCTACCACAGACGTTCTTGAACAAAGAATCGCCGCTTTAGAAGGCGGAGTTGCAGCG
CTTGCTACTGCTTCCGGTCAAGCGGCGGAAACTCTCGCATTACTAAACATAGTAGAAGCTGGTCAAGAAATTGTGGCCTC
TTCTTCCCTTTACGGCGGAACTTATAACCTCCTTCATTATACGTTTCCTAAATTAGGAATTAAGGTACATTTTGTAGATC
CCTCGGATCCTGAAAATTTTCGCAAAGCAGTCAATGATAAAACCAGAGCATTTTACGCAGAAACATTAGGAAATCCTAAA
TTAGATACTTTGAATCTGGAAGCGATTGCAAAAGTTGCACACGATTCGGAAGTCCCTTTGATAATAGACAATACCTTACC
CTCTCCCTATTTAGTCAATCCGATCGAACACGGTGCAGATATTGTAGTTCATTCTCTAACTAAATTTTTAGGAGGGCACG
GAACTTCAATCGGAGGTATTATAGTCGATTCCGGTAAGTTTAATTGGGGAAACGGAAAGTTTAAAAATTTTACAGAGCCA
GACCCAAGTTATCACGGTTTAAAGTTCTGGGAAGTTTTTGGTAAGTTCGAACCGTTCGGCGGAGTAAATATCGCTTACAT
CATCAAAGCAAAAGTACAAGGACTTCGTGATATGGGTGCCTCTATTTCTCCTTTTAACGCCTGGCAAATTCTACAAGGTG
TGGAAACTCTTCCTCTTAGAATGCGTAAACATTCCGAAAACGCTCTTGCAGTCGCAGAATATCTTACCAAACATACTAAA
GTTTCTTGGGTAAACTACCCAGGTTTAAAAATGGATAAGAACTATTCTCTTGCAAAGAAATATCATAAAAAAGATCTTTA
CGGTGCCATTCTAGGTTTTGGGATTAAAGGTGGAGCTGTAGAAGCAAAGAAATTCATAGACGGGTTAGAATTATTTTCCC
TTTTGGCAAACGTAGGAGATGCAAAATCTTTGGTGATCCATCCAGCGTCTACGACTCACCAGCAGTTAACTCCGGAAGAA
CAACTTTCAGCTGGAGTTACTCCTGACTTTGTTCGTCTTTCCGTCGGTTTGGAAAATATAGAAGATATTTTATTCGATTT
AGAGGAAGCTCTGAAGAAAGTTTGA

Upstream 100 bases:

>100_bases
TCTCATTCGTATAAACAGAATTCAATTTCAATATAACGGATTTTAGTTTGACTTCTGGGCAAAAATTCTCAGAATACGAA
AAAGGAGAATTAAAACCAAA

Downstream 100 bases:

>100_bases
TAGTAAAGAGGTTTTGATTTTTTTGTATTTCAAAGGAGTTAAGTTCCTTTGATTTATCTATAGAAAGTAAGTATTCAACA
GTAGAGCGACAACTCAAATG

Product: O-acetylhomoserine (thiol) lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPRNYKPETIALHGGQSPDPSTLSRAVPIYQTTSYVFKNTEHAAKLFGLQEFGNIYTRIMNPTTDVLEQRIAALEGGVAA
LATASGQAAETLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSDPENFRKAVNDKTRAFYAETLGNPK
LDTLNLEAIAKVAHDSEVPLIIDNTLPSPYLVNPIEHGADIVVHSLTKFLGGHGTSIGGIIVDSGKFNWGNGKFKNFTEP
DPSYHGLKFWEVFGKFEPFGGVNIAYIIKAKVQGLRDMGASISPFNAWQILQGVETLPLRMRKHSENALAVAEYLTKHTK
VSWVNYPGLKMDKNYSLAKKYHKKDLYGAILGFGIKGGAVEAKKFIDGLELFSLLANVGDAKSLVIHPASTTHQQLTPEE
QLSAGVTPDFVRLSVGLENIEDILFDLEEALKKV

Sequences:

>Translated_434_residues
MPRNYKPETIALHGGQSPDPSTLSRAVPIYQTTSYVFKNTEHAAKLFGLQEFGNIYTRIMNPTTDVLEQRIAALEGGVAA
LATASGQAAETLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSDPENFRKAVNDKTRAFYAETLGNPK
LDTLNLEAIAKVAHDSEVPLIIDNTLPSPYLVNPIEHGADIVVHSLTKFLGGHGTSIGGIIVDSGKFNWGNGKFKNFTEP
DPSYHGLKFWEVFGKFEPFGGVNIAYIIKAKVQGLRDMGASISPFNAWQILQGVETLPLRMRKHSENALAVAEYLTKHTK
VSWVNYPGLKMDKNYSLAKKYHKKDLYGAILGFGIKGGAVEAKKFIDGLELFSLLANVGDAKSLVIHPASTTHQQLTPEE
QLSAGVTPDFVRLSVGLENIEDILFDLEEALKKV
>Mature_433_residues
PRNYKPETIALHGGQSPDPSTLSRAVPIYQTTSYVFKNTEHAAKLFGLQEFGNIYTRIMNPTTDVLEQRIAALEGGVAAL
ATASGQAAETLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGIKVHFVDPSDPENFRKAVNDKTRAFYAETLGNPKL
DTLNLEAIAKVAHDSEVPLIIDNTLPSPYLVNPIEHGADIVVHSLTKFLGGHGTSIGGIIVDSGKFNWGNGKFKNFTEPD
PSYHGLKFWEVFGKFEPFGGVNIAYIIKAKVQGLRDMGASISPFNAWQILQGVETLPLRMRKHSENALAVAEYLTKHTKV
SWVNYPGLKMDKNYSLAKKYHKKDLYGAILGFGIKGGAVEAKKFIDGLELFSLLANVGDAKSLVIHPASTTHQQLTPEEQ
LSAGVTPDFVRLSVGLENIEDILFDLEEALKKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=425, Percent_Identity=31.5294117647059, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI299473759, Length=425, Percent_Identity=28.4705882352941, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=28.3018867924528, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1790375, Length=428, Percent_Identity=28.9719626168224, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789383, Length=439, Percent_Identity=24.1457858769932, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17538055, Length=428, Percent_Identity=32.0093457943925, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=30.1932367149758, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=428, Percent_Identity=31.0747663551402, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=449, Percent_Identity=46.9933184855234, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6319307, Length=383, Percent_Identity=33.420365535248, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=451, Percent_Identity=26.8292682926829, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=363, Percent_Identity=25.068870523416, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=31.615925058548, Blast_Score=197, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47469; Mature: 47338

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNYKPETIALHGGQSPDPSTLSRAVPIYQTTSYVFKNTEHAAKLFGLQEFGNIYTRIM
CCCCCCCCEEEEECCCCCCHHHHHHCCCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NPTTDVLEQRIAALEGGVAALATASGQAAETLALLNIVEAGQEIVASSSLYGGTYNLLHY
CCHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHE
TFPKLGIKVHFVDPSDPENFRKAVNDKTRAFYAETLGNPKLDTLNLEAIAKVAHDSEVPL
ECCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCE
IIDNTLPSPYLVNPIEHGADIVVHSLTKFLGGHGTSIGGIIVDSGKFNWGNGKFKNFTEP
EEECCCCCCEEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
DPSYHGLKFWEVFGKFEPFGGVNIAYIIKAKVQGLRDMGASISPFNAWQILQGVETLPLR
CCCCCCCHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHH
MRKHSENALAVAEYLTKHTKVSWVNYPGLKMDKNYSLAKKYHKKDLYGAILGFGIKGGAV
HHHCCCCHHHHHHHHHHCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCH
EAKKFIDGLELFSLLANVGDAKSLVIHPASTTHQQLTPEEQLSAGVTPDFVRLSVGLENI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCCEEEECCHHHH
EDILFDLEEALKKV
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PRNYKPETIALHGGQSPDPSTLSRAVPIYQTTSYVFKNTEHAAKLFGLQEFGNIYTRIM
CCCCCCCEEEEECCCCCCHHHHHHCCCCEECHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NPTTDVLEQRIAALEGGVAALATASGQAAETLALLNIVEAGQEIVASSSLYGGTYNLLHY
CCHHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHE
TFPKLGIKVHFVDPSDPENFRKAVNDKTRAFYAETLGNPKLDTLNLEAIAKVAHDSEVPL
ECCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCE
IIDNTLPSPYLVNPIEHGADIVVHSLTKFLGGHGTSIGGIIVDSGKFNWGNGKFKNFTEP
EEECCCCCCEEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
DPSYHGLKFWEVFGKFEPFGGVNIAYIIKAKVQGLRDMGASISPFNAWQILQGVETLPLR
CCCCCCCHHHHHHCCCCCCCCCEEEEEEEHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHH
MRKHSENALAVAEYLTKHTKVSWVNYPGLKMDKNYSLAKKYHKKDLYGAILGFGIKGGAV
HHHCCCCHHHHHHHHHHCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCH
EAKKFIDGLELFSLLANVGDAKSLVIHPASTTHQQLTPEEQLSAGVTPDFVRLSVGLENI
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCCCCCCEEEECCHHHH
EDILFDLEEALKKV
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]