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The map label for this gene is nth

Identifier: 45657625

GI number: 45657625

Start: 2151822

End: 2152520

Strand: Reverse

Name: nth

Synonym: LIC11759

Alternate gene names: 45657625

Gene position: 2152520-2151822 (Counterclockwise)

Preceding gene: 45657626

Following gene: 45657624

Centisome position: 50.33

GC content: 36.91

Gene sequence:

>699_bases
TTGCTCCGAAAAAATACGCAGGAATCACAAAACAGGCACCTAAAAAAGCCTGATCCTGCGTTTCTAAAATGGTTTTCCCG
AATTTTTTCTCTTTTAAGAAAAGAATTCGGGGAAGTCTCTACACCTCTTCACTTTCAAAAAGATTATGAACTTGCGATTG
CGGTCATTTTATCCGCTCAATGTACGGATGAACGAGTCAACCAAGTTACTCCCGCCCTTTTTAAAGCCTTTCCTACATTA
GAATCCTTTGCCAGTTCCGATTTAAAAACTATTGAAACCCTCATTTTTTCTACGGGTTTTTATAGAAATAAAGCAAAGTC
TATCCAAGGTTTTGCAAAGAAATTGTTAAACGACTTCGACGGTAAAATTCCAAAAACAATTCCAGAACTGATCACACTTC
CTGGTTTTGGTCGTAAAACTGCAAATGTGGTTTTATCGGAAGTTCACGGACTTGTAGAAGGAATTGTAGTAGATACTCAC
GTAAACAGGCTTTCAAAAGTTCTAGGACTTACTACTAAAAACGATCCGGTTCAAGTGGAGAAGGATTTAATGTCCCTTCT
TCCGGAAAAATATTGGAGGGATATTTCTTTGTATTTGATTTTTTTAGGAAGAAAAAGTTGTAAGGCGCATCGTAGATTTT
GCGAGGATTGTATCTTAAAAAAAGATTGCCCATCGTCTTCTATTATATCAGGAGTTTAG

Upstream 100 bases:

>100_bases
CGCTGGGTTACAATCCGTCTTTCTACCAGAGCTCTTAGAACTCTGAGAAAAAAAGGAATTAAGGCCGCCATCAAAGATAA
TGGTGGATCCTTGGGCGTTC

Downstream 100 bases:

>100_bases
AATATTATGGAAGTATTTATCGATCAGAAGTTAGAAGGAATGGAACTTGCTACTCAACATATCGTGATGTCCAGGGATCT
AAATCAACACGGTTTTCTTT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGEVSTPLHFQKDYELAIAVILSAQCTDERVNQVTPALFKAFPTL
ESFASSDLKTIETLIFSTGFYRNKAKSIQGFAKKLLNDFDGKIPKTIPELITLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRLSKVLGLTTKNDPVQVEKDLMSLLPEKYWRDISLYLIFLGRKSCKAHRRFCEDCILKKDCPSSSIISGV

Sequences:

>Translated_232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGEVSTPLHFQKDYELAIAVILSAQCTDERVNQVTPALFKAFPTL
ESFASSDLKTIETLIFSTGFYRNKAKSIQGFAKKLLNDFDGKIPKTIPELITLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRLSKVLGLTTKNDPVQVEKDLMSLLPEKYWRDISLYLIFLGRKSCKAHRRFCEDCILKKDCPSSSIISGV
>Mature_232_residues
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGEVSTPLHFQKDYELAIAVILSAQCTDERVNQVTPALFKAFPTL
ESFASSDLKTIETLIFSTGFYRNKAKSIQGFAKKLLNDFDGKIPKTIPELITLPGFGRKTANVVLSEVHGLVEGIVVDTH
VNRLSKVLGLTTKNDPVQVEKDLMSLLPEKYWRDISLYLIFLGRKSCKAHRRFCEDCILKKDCPSSSIISGV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=182, Percent_Identity=30.7692307692308, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1787920, Length=196, Percent_Identity=35.2040816326531, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554540, Length=193, Percent_Identity=35.7512953367876, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324530, Length=189, Percent_Identity=31.2169312169312, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319304, Length=196, Percent_Identity=31.6326530612245, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550361, Length=189, Percent_Identity=29.6296296296296, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26307; Mature: 26307

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGEVSTPLHFQKDYELAIAVILSAQ
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
CTDERVNQVTPALFKAFPTLESFASSDLKTIETLIFSTGFYRNKAKSIQGFAKKLLNDFD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GKIPKTIPELITLPGFGRKTANVVLSEVHGLVEGIVVDTHVNRLSKVLGLTTKNDPVQVE
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDLMSLLPEKYWRDISLYLIFLGRKSCKAHRRFCEDCILKKDCPSSSIISGV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MLRKNTQESQNRHLKKPDPAFLKWFSRIFSLLRKEFGEVSTPLHFQKDYELAIAVILSAQ
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
CTDERVNQVTPALFKAFPTLESFASSDLKTIETLIFSTGFYRNKAKSIQGFAKKLLNDFD
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GKIPKTIPELITLPGFGRKTANVVLSEVHGLVEGIVVDTHVNRLSKVLGLTTKNDPVQVE
CCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KDLMSLLPEKYWRDISLYLIFLGRKSCKAHRRFCEDCILKKDCPSSSIISGV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]