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The map label for this gene is leuA

Identifier: 45657592

GI number: 45657592

Start: 2100758

End: 2102344

Strand: Direct

Name: leuA

Synonym: LIC11726

Alternate gene names: 45657592

Gene position: 2100758-2102344 (Clockwise)

Preceding gene: 45657590

Following gene: 45657593

Centisome position: 49.12

GC content: 42.22

Gene sequence:

>1587_bases
ATGAGTGTTCGTTTAATTGATTGTCTAAAGAATAAAACCCAAAAAACTGGAAGTAGAATTTTTTGCGAGAAAGATATGGC
AATGGATCTAAAAGACTACGTTCGTATATTTGATACAACTCTCAGAGACGGAGAACAATGTCCCGGGGCTGCGATGACCG
AAAACGAAAAGTTGGAAATTGCTTCTCAACTCGCAACGATGAAGGTAGATATTATTGAAGCCGGATTTCCAGTTTCTTCC
CCCGTTCAATTTCAAGCAGTGGAAAGAATCGCAAGAGAAACCGAAGGGCCCATGATCGCGGCACTCGCTAGGGCAATGAA
GGCGGATATTGAAGCCGCATCTAAAGCGCTTCAGCCTGCGAAAAAAAGAAGGATTCATACTTTCATTGCATCTTCTCCGA
TTCACATGAAATATAAACTCGGAAAAGAACCGAAAGAAGTTTTAAAGATGGCTGTAGAAGCGGTGACACTTTGCAGGCAA
TTTGTGGACGACGTGGAATTTTCACCCGAAGACGCAACTAGGAGCGAACCTGAATTTTTAAGAGAACTTTGTGAAGCGGT
AATTGCAGCAGGAGCTACTACGATCAACATTCCTGACACTGTAGGTTATACCACTCCTGCAGAATACGGTGGTCTTTTTA
AATTTCTGCTCAGCAACGTAAGAGGAGCCGAAAAAATTATCTTTTCTGCTCATTGTCATAACGATTTAGGACTTGCTACC
GCAAATTCCTTGGCTGCGGTTCAAAACGGAGCTAGACAAATTGAATGTACGATTAACGGTATCGGAGAAAGAGCCGGTAA
TACAGCAATGGAAGAAGTAGTTATGGCAATGCGTACTAGAAAAGATACGTTCGGAATTCAAACTCAAATTAAAACCGAAG
AAATTGCCCGCGCCTCTTATCTTGTAAAAACGATTACCGGAATGCTCGTTCAACCGAACAAAGCAATCGTAGGAGCAAAC
GCATTTGCACATGAATCTGGAATTCATCAAGACGGAGTTATCAAACATAGAGAAACCTACGAGATTATGAAACCGGAAAC
CGTCGGTCTTTCTTCCAATAGAATGGTTTTGGGAAGACATAGTGGTCGCGCCGGTTTTAAAGATAGAATTGTTAAACTGG
GTTTTTCTCCTCAAGTAGAAGAATTGGAAGCCGCTTATCAAAGGTTTTTAGAAATTGCGGACAGAAAAAAAGAAATCTAT
GATGAAGACATTCGAGCGCTTTTCTCCGAAGAAGCTAGAAAATCAACCGGAGATCGTTTTCAATTAGAAGGTTTTACGGT
TTCCACCGGAACCAAAAGTACTCCCACTGCGGGTGTTAGAATTTTAATTGATGGTCATGTAAGAGAAGAGTCTGCAACCG
GAGACGGCCCGGTAGACGCAATCTACAAAGCGATTCAAAAAACAACCGGAATGGATCCGGAAGTCTCCAGACTTGTAATT
TCTCCGGTTACCGAAGGACAAGATGCAATGGCTGAAGCCTCCGTGACTTTAGAATATAAAGGAGACCGTGTCGTGGGAAA
AGGAAGTTCTACAGATATTATCGAAGCCTGTTCAAGGGCTTATATTTCCGCACTCAATCGACTTTGA

Upstream 100 bases:

>100_bases
GATTGAAAGTTAGACGCGTAGAATTGAACCATTCTTTTAGAAAAGAGTATAATAATAAAAAAAGAAATTATTATTTTTCA
ATCGATTATTTTCGTTTTAA

Downstream 100 bases:

>100_bases
ATATATTCTTATTTAAGAATATATTCAAATAATAATTATATAATATCTAATTACTAAATTTTAAAACTATTTAAAACTAC
ATAATAAAATACTTATTTGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSVRLIDCLKNKTQKTGSRIFCEKDMAMDLKDYVRIFDTTLRDGEQCPGAAMTENEKLEIASQLATMKVDIIEAGFPVSS
PVQFQAVERIARETEGPMIAALARAMKADIEAASKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCRQ
FVDDVEFSPEDATRSEPEFLRELCEAVIAAGATTINIPDTVGYTTPAEYGGLFKFLLSNVRGAEKIIFSAHCHNDLGLAT
ANSLAAVQNGARQIECTINGIGERAGNTAMEEVVMAMRTRKDTFGIQTQIKTEEIARASYLVKTITGMLVQPNKAIVGAN
AFAHESGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQVEELEAAYQRFLEIADRKKEIY
DEDIRALFSEEARKSTGDRFQLEGFTVSTGTKSTPTAGVRILIDGHVREESATGDGPVDAIYKAIQKTTGMDPEVSRLVI
SPVTEGQDAMAEASVTLEYKGDRVVGKGSSTDIIEACSRAYISALNRL

Sequences:

>Translated_528_residues
MSVRLIDCLKNKTQKTGSRIFCEKDMAMDLKDYVRIFDTTLRDGEQCPGAAMTENEKLEIASQLATMKVDIIEAGFPVSS
PVQFQAVERIARETEGPMIAALARAMKADIEAASKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCRQ
FVDDVEFSPEDATRSEPEFLRELCEAVIAAGATTINIPDTVGYTTPAEYGGLFKFLLSNVRGAEKIIFSAHCHNDLGLAT
ANSLAAVQNGARQIECTINGIGERAGNTAMEEVVMAMRTRKDTFGIQTQIKTEEIARASYLVKTITGMLVQPNKAIVGAN
AFAHESGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQVEELEAAYQRFLEIADRKKEIY
DEDIRALFSEEARKSTGDRFQLEGFTVSTGTKSTPTAGVRILIDGHVREESATGDGPVDAIYKAIQKTTGMDPEVSRLVI
SPVTEGQDAMAEASVTLEYKGDRVVGKGSSTDIIEACSRAYISALNRL
>Mature_527_residues
SVRLIDCLKNKTQKTGSRIFCEKDMAMDLKDYVRIFDTTLRDGEQCPGAAMTENEKLEIASQLATMKVDIIEAGFPVSSP
VQFQAVERIARETEGPMIAALARAMKADIEAASKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCRQF
VDDVEFSPEDATRSEPEFLRELCEAVIAAGATTINIPDTVGYTTPAEYGGLFKFLLSNVRGAEKIIFSAHCHNDLGLATA
NSLAAVQNGARQIECTINGIGERAGNTAMEEVVMAMRTRKDTFGIQTQIKTEEIARASYLVKTITGMLVQPNKAIVGANA
FAHESGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQVEELEAAYQRFLEIADRKKEIYD
EDIRALFSEEARKSTGDRFQLEGFTVSTGTKSTPTAGVRILIDGHVREESATGDGPVDAIYKAIQKTTGMDPEVSRLVIS
PVTEGQDAMAEASVTLEYKGDRVVGKGSSTDIIEACSRAYISALNRL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=495, Percent_Identity=46.0606060606061, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320071, Length=389, Percent_Identity=27.2493573264781, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=379, Percent_Identity=27.4406332453826, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=544, Percent_Identity=25.3676470588235, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=543, Percent_Identity=25.0460405156538, Blast_Score=129, Evalue=8e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57844; Mature: 57713

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRLIDCLKNKTQKTGSRIFCEKDMAMDLKDYVRIFDTTLRDGEQCPGAAMTENEKLEI
CCCCHHHHHHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ASQLATMKVDIIEAGFPVSSPVQFQAVERIARETEGPMIAALARAMKADIEAASKALQPA
HHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHH
KKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCRQFVDDVEFSPEDATRSEPEFL
HHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RELCEAVIAAGATTINIPDTVGYTTPAEYGGLFKFLLSNVRGAEKIIFSAHCHNDLGLAT
HHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHH
ANSLAAVQNGARQIECTINGIGERAGNTAMEEVVMAMRTRKDTFGIQTQIKTEEIARASY
HHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHCCCEEECCHHHHHHHHH
LVKTITGMLVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRH
HHHHHHHHEECCCCEEEECCHHHHCCCCCCCCCCCCCCHHHHCCCCEECCCCCCEEEECC
SGRAGFKDRIVKLGFSPQVEELEAAYQRFLEIADRKKEIYDEDIRALFSEEARKSTGDRF
CCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QLEGFTVSTGTKSTPTAGVRILIDGHVREESATGDGPVDAIYKAIQKTTGMDPEVSRLVI
EEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEE
SPVTEGQDAMAEASVTLEYKGDRVVGKGSSTDIIEACSRAYISALNRL
CCCCCCCHHHHHCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVRLIDCLKNKTQKTGSRIFCEKDMAMDLKDYVRIFDTTLRDGEQCPGAAMTENEKLEI
CCCHHHHHHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ASQLATMKVDIIEAGFPVSSPVQFQAVERIARETEGPMIAALARAMKADIEAASKALQPA
HHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHH
KKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCRQFVDDVEFSPEDATRSEPEFL
HHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RELCEAVIAAGATTINIPDTVGYTTPAEYGGLFKFLLSNVRGAEKIIFSAHCHNDLGLAT
HHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCHH
ANSLAAVQNGARQIECTINGIGERAGNTAMEEVVMAMRTRKDTFGIQTQIKTEEIARASY
HHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHCCCEEECCHHHHHHHHH
LVKTITGMLVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRH
HHHHHHHHEECCCCEEEECCHHHHCCCCCCCCCCCCCCHHHHCCCCEECCCCCCEEEECC
SGRAGFKDRIVKLGFSPQVEELEAAYQRFLEIADRKKEIYDEDIRALFSEEARKSTGDRF
CCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QLEGFTVSTGTKSTPTAGVRILIDGHVREESATGDGPVDAIYKAIQKTTGMDPEVSRLVI
EEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHEEE
SPVTEGQDAMAEASVTLEYKGDRVVGKGSSTDIIEACSRAYISALNRL
CCCCCCCHHHHHCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA