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The map label for this gene is yngF [H]

Identifier: 45657540

GI number: 45657540

Start: 2034761

End: 2035528

Strand: Direct

Name: yngF [H]

Synonym: LIC11672

Alternate gene names: 45657540

Gene position: 2034761-2035528 (Clockwise)

Preceding gene: 45657539

Following gene: 45657543

Centisome position: 47.57

GC content: 36.46

Gene sequence:

>768_bases
ATGCTTTCCGAAATCAAAAATGATCATATCTTGGAACTTTATATTGAAACTAACGAAGTGAATTCTTTGAACGTAGAATT
TTTCAAAACATTCTCTGCAAAACTGGATTTGATTGCTAAGGATCAGTCGATCAAATCGGTCATCTTAACTTCTAAAAACG
AAAAGTTTTTTTCTAATGGTTTTAATCCAGAAATTTTTGTAGATAAATCTTCGGAAGAAATCAAAAACGTTATGCGTATC
GCTTTGGAAACTGCATCCAAATATTTATTTTTGGATAGACCCGTAGTTTGTGCGATGAACGGACATTCTATGGGTTTAGG
TGCTGTGTTTGCGATCTTTTCTGATTATAGAATTATGGTGGAAAAAAAAGGAAGATTGGGTTTTCCAGAATCGCAGATCG
GAATTAATTTTCCTGCTGTTGCCGGTTTTATGCTTAAGGAGGTTGTAGGAATTACGAAAGCAAGAGATCTTTTGTATTCC
GGAAAAGCTCTCAAAGCGGAAGAAGCTCTGGAAATCAGTTTGATTGATGAGGTTGCTTCTTCTTCGGATGATCTTATAAC
TCGTGCTCGAAAATATTGTGATCAGTTCAAGAATATGGCGATCGGTTCCGTGGTTGGAATTAAGGTTTCTTTGCGTGATC
CGGTTCGTATTTTTGCGGAACATAACGCAGAAAGGGATGTGGAACTGATTTCAAAGGCTATATTTTCTAAAAACGGCCAA
GAAGGTATGAAATCTATCTTGGAAAAAAGAAGGCCCGTATTTCAGTAA

Upstream 100 bases:

>100_bases
TTTAATAGATAATAGACTATTTCTGAGAAATATATTCTTTAATTTATTTGAGATTTACAAAAAAATTATAAACTAGGTTT
TTAAATAAGGAGAAACGAAT

Downstream 100 bases:

>100_bases
ATTATAATTTAAAAAAGAAGAATGAAAAAACGGCCGACGTTTTGACCTGCCTTTTTTATTAATTCTTATTCTAGGCATTT
CATTTTTTTCGAATTGAGTT

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLSEIKNDHILELYIETNEVNSLNVEFFKTFSAKLDLIAKDQSIKSVILTSKNEKFFSNGFNPEIFVDKSSEEIKNVMRI
ALETASKYLFLDRPVVCAMNGHSMGLGAVFAIFSDYRIMVEKKGRLGFPESQIGINFPAVAGFMLKEVVGITKARDLLYS
GKALKAEEALEISLIDEVASSSDDLITRARKYCDQFKNMAIGSVVGIKVSLRDPVRIFAEHNAERDVELISKAIFSKNGQ
EGMKSILEKRRPVFQ

Sequences:

>Translated_255_residues
MLSEIKNDHILELYIETNEVNSLNVEFFKTFSAKLDLIAKDQSIKSVILTSKNEKFFSNGFNPEIFVDKSSEEIKNVMRI
ALETASKYLFLDRPVVCAMNGHSMGLGAVFAIFSDYRIMVEKKGRLGFPESQIGINFPAVAGFMLKEVVGITKARDLLYS
GKALKAEEALEISLIDEVASSSDDLITRARKYCDQFKNMAIGSVVGIKVSLRDPVRIFAEHNAERDVELISKAIFSKNGQ
EGMKSILEKRRPVFQ
>Mature_255_residues
MLSEIKNDHILELYIETNEVNSLNVEFFKTFSAKLDLIAKDQSIKSVILTSKNEKFFSNGFNPEIFVDKSSEEIKNVMRI
ALETASKYLFLDRPVVCAMNGHSMGLGAVFAIFSDYRIMVEKKGRLGFPESQIGINFPAVAGFMLKEVVGITKARDLLYS
GKALKAEEALEISLIDEVASSSDDLITRARKYCDQFKNMAIGSVVGIKVSLRDPVRIFAEHNAERDVELISKAIFSKNGQ
EGMKSILEKRRPVFQ

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=27.5280898876405, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=23.6842105263158, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787659, Length=236, Percent_Identity=24.5762711864407, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788682, Length=181, Percent_Identity=23.7569060773481, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=27.3684210526316, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583165, Length=189, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921018, Length=192, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28603; Mature: 28603

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEIKNDHILELYIETNEVNSLNVEFFKTFSAKLDLIAKDQSIKSVILTSKNEKFFSNG
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCHHEEEEECCCCHHHHCC
FNPEIFVDKSSEEIKNVMRIALETASKYLFLDRPVVCAMNGHSMGLGAVFAIFSDYRIMV
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHCCEEEE
EKKGRLGFPESQIGINFPAVAGFMLKEVVGITKARDLLYSGKALKAEEALEISLIDEVAS
EECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
SSDDLITRARKYCDQFKNMAIGSVVGIKVSLRDPVRIFAEHNAERDVELISKAIFSKNGQ
CCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCH
EGMKSILEKRRPVFQ
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSEIKNDHILELYIETNEVNSLNVEFFKTFSAKLDLIAKDQSIKSVILTSKNEKFFSNG
CCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHEEEEECCCCHHEEEEECCCCHHHHCC
FNPEIFVDKSSEEIKNVMRIALETASKYLFLDRPVVCAMNGHSMGLGAVFAIFSDYRIMV
CCCEEEEECCHHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHHCCEEEE
EKKGRLGFPESQIGINFPAVAGFMLKEVVGITKARDLLYSGKALKAEEALEISLIDEVAS
EECCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
SSDDLITRARKYCDQFKNMAIGSVVGIKVSLRDPVRIFAEHNAERDVELISKAIFSKNGQ
CCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHCCCCH
EGMKSILEKRRPVFQ
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]