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The map label for this gene is lipA

Identifier: 45657518

GI number: 45657518

Start: 2012170

End: 2013075

Strand: Reverse

Name: lipA

Synonym: LIC11646

Alternate gene names: 45657518

Gene position: 2013075-2012170 (Counterclockwise)

Preceding gene: 45657521

Following gene: 45657517

Centisome position: 47.07

GC content: 37.97

Gene sequence:

>906_bases
ATGAATCCTCTTAAAAAAAAACCTCGTACTCATTCTCTTCAAAATGCTCCTGAAAAACCAGATTGGCTCAAAGTAAAACT
CGCCTTTCCTGATCCAAAAAATAACCCTGTCGCAATTGTAAGAAATTCTCTAGAAGAAAAAAAACTCAATACAGTTTGCG
AAAGCGCTTCTTGCCCTAACCTAAATCACTGCTGGTCCAGAAAAACAGCGACTTATATGTTAGGTGGAGATATTTGTACA
AGACGTTGTTCCTATTGTGACGTAGCTTCTGGAAAACCTTTTCCTCTGGATCCAGAAGAACCTAAAAGGATAGCAGAATC
TTCGATCGCTTTAGGTCTCAGACACGTAGTCATTACCTCGGTTAATAGAGACGATCTTGAAGACGGAGGCGCAGCTCATT
TTGCAAAAACCGTAAAAGAAATCCGTAAAGGACTTCCAGATTGTAAAATAGAACTTTTGATTCCAGATCTGAAAGTAAAA
CAAGAGGCTTTAGAAATTATTTTTGAATGTAATCCTGATATATTCAATCATAACTTAGAAACGGTAAAAAGACTTTTTCC
AGAAGTGGCTCCTCAAAAAAGATATGAACGTTCTTTAGACGTTTTGAAAATTGCTTCTGCAAGAGGATTTCTGACAAAAA
GTGGTTTAATTTTGGGTATGGGAGAAACTTTAGAAGAAGTAAAAGAATGCATGCAAGATCTTGCAAGTGTTGGAGTTTCC
CTTTTGACACTGGGACAGTATTTACAACCGACTTCGACTCATCTTCCCGTAAAAGAATACGTAGTTCCCCAAGTATTTAA
GGATTTACGAATATACGGAAAGTCCATCGGTTTTAAGGGAGTTTTTTCAGGACCCCTTGTAAGAAGTTCTTATCACGCGG
ACGAACAAATCTCATGGAACCCGTAG

Upstream 100 bases:

>100_bases
AAAAATCTTCTACCTAAAACTTTCGTTGACCGGTTTTACAAATATTCAAAGGATAGAAGGAAAGACTTTGCCATTTATCA
CGTAAAAAGTATAGAATTGT

Downstream 100 bases:

>100_bases
AAAATTCTCACCTAGGTCCGCCTTCCGAAGAAATTAAAAAACTTATCTATCACTCCATAATTCGATTTCTTTCTAACAGA
GAAACTCCGATTAGTAGATC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MNPLKKKPRTHSLQNAPEKPDWLKVKLAFPDPKNNPVAIVRNSLEEKKLNTVCESASCPNLNHCWSRKTATYMLGGDICT
RRCSYCDVASGKPFPLDPEEPKRIAESSIALGLRHVVITSVNRDDLEDGGAAHFAKTVKEIRKGLPDCKIELLIPDLKVK
QEALEIIFECNPDIFNHNLETVKRLFPEVAPQKRYERSLDVLKIASARGFLTKSGLILGMGETLEEVKECMQDLASVGVS
LLTLGQYLQPTSTHLPVKEYVVPQVFKDLRIYGKSIGFKGVFSGPLVRSSYHADEQISWNP

Sequences:

>Translated_301_residues
MNPLKKKPRTHSLQNAPEKPDWLKVKLAFPDPKNNPVAIVRNSLEEKKLNTVCESASCPNLNHCWSRKTATYMLGGDICT
RRCSYCDVASGKPFPLDPEEPKRIAESSIALGLRHVVITSVNRDDLEDGGAAHFAKTVKEIRKGLPDCKIELLIPDLKVK
QEALEIIFECNPDIFNHNLETVKRLFPEVAPQKRYERSLDVLKIASARGFLTKSGLILGMGETLEEVKECMQDLASVGVS
LLTLGQYLQPTSTHLPVKEYVVPQVFKDLRIYGKSIGFKGVFSGPLVRSSYHADEQISWNP
>Mature_301_residues
MNPLKKKPRTHSLQNAPEKPDWLKVKLAFPDPKNNPVAIVRNSLEEKKLNTVCESASCPNLNHCWSRKTATYMLGGDICT
RRCSYCDVASGKPFPLDPEEPKRIAESSIALGLRHVVITSVNRDDLEDGGAAHFAKTVKEIRKGLPDCKIELLIPDLKVK
QEALEIIFECNPDIFNHNLETVKRLFPEVAPQKRYERSLDVLKIASARGFLTKSGLILGMGETLEEVKECMQDLASVGVS
LLTLGQYLQPTSTHLPVKEYVVPQVFKDLRIYGKSIGFKGVFSGPLVRSSYHADEQISWNP

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=42.1052631578947, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=40.8906882591093, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1786846, Length=281, Percent_Identity=47.3309608540925, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=43.859649122807, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=304, Percent_Identity=40.4605263157895, Blast_Score=223, Evalue=2e-59,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=43.5087719298246, Blast_Score=228, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_LEPIC (Q72RU2)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001604.1
- ProteinModelPortal:   Q72RU2
- GeneID:   2771666
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11646
- NMPDR:   fig|267671.1.peg.1604
- HOGENOM:   HBG284542
- OMA:   TICEEAC
- ProtClustDB:   PRK05481
- BioCyc:   LINT267671:LIC_11646-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33666; Mature: 33666

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLKKKPRTHSLQNAPEKPDWLKVKLAFPDPKNNPVAIVRNSLEEKKLNTVCESASCPN
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
LNHCWSRKTATYMLGGDICTRRCSYCDVASGKPFPLDPEEPKRIAESSIALGLRHVVITS
CHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
VNRDDLEDGGAAHFAKTVKEIRKGLPDCKIELLIPDLKVKQEALEIIFECNPDIFNHNLE
CCHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHCCCHH
TVKRLFPEVAPQKRYERSLDVLKIASARGFLTKSGLILGMGETLEEVKECMQDLASVGVS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHH
LLTLGQYLQPTSTHLPVKEYVVPQVFKDLRIYGKSIGFKGVFSGPLVRSSYHADEQISWN
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
P
C
>Mature Secondary Structure
MNPLKKKPRTHSLQNAPEKPDWLKVKLAFPDPKNNPVAIVRNSLEEKKLNTVCESASCPN
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
LNHCWSRKTATYMLGGDICTRRCSYCDVASGKPFPLDPEEPKRIAESSIALGLRHVVITS
CHHHHCCCCEEEEECCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEC
VNRDDLEDGGAAHFAKTVKEIRKGLPDCKIELLIPDLKVKQEALEIIFECNPDIFNHNLE
CCHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHHCCCHH
TVKRLFPEVAPQKRYERSLDVLKIASARGFLTKSGLILGMGETLEEVKECMQDLASVGVS
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCEEEECCHHHHHHHHHHHHHHHHHHH
LLTLGQYLQPTSTHLPVKEYVVPQVFKDLRIYGKSIGFKGVFSGPLVRSSYHADEQISWN
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA