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The map label for this gene is amaA

Identifier: 45657198

GI number: 45657198

Start: 1627295

End: 1628476

Strand: Reverse

Name: amaA

Synonym: LIC11318

Alternate gene names: 45657198

Gene position: 1628476-1627295 (Counterclockwise)

Preceding gene: 45657200

Following gene: 45657195

Centisome position: 38.07

GC content: 38.92

Gene sequence:

>1182_bases
ATGAAACACACTCTAACTTCTAATAGAATGGCTGAACTCATTCGATACAGAAGACAAATTCACAAACATCCAGAACTTAG
ATATGAAGAAAATCAAACCTCTGACTACGTAATCAATCATCTCAAAGAATTAGGTCTTTCTTTTCAGGATAAGATCGCAA
AAACCGGAGTAGTATCTTTAATCGATTCTGGTAAACCAGGAAAAACCCTTCTTGTACGTGCAGACATGGACGCTCTTCCT
ATATTCGAAGAATCTAATCAAGAATACAAATCGGTTCACGAAGGTGTGATGCACGCTTGTGGTCACGACGCTCATACTTC
CATTCTTATGGGACTGGCAACGGAAATCAAAGAGAATATCCAATTCATTCTTCCCAAAGGAAAGGTGTTACTGGTATTTC
AACCAGCAGAAGAAGGTGGTCAAGGTGCTGACAGAATGATTGAAGAAGGTATATTAGAAAAATATAATGTAGATGCTGCC
CTAGCCCTACACGTATGGAATCATATTCCAATTGGAAAAATAGGAGTTGTAGATGGCGCTATGATGGCGGCTGTTGACGA
ATTTACGATTACAATATCAGGAATCAGTGGACACGGCGCCATGCCTCAACACACAGTGGACCCAATTGTAGTAGGTGCTC
AAATCGTAAACTCGTTACAAACCATCGTTTCTAGAAATACTGACCCTTTAGATTCCTGCGTAGTAACCGTAGGAAGTTTT
CATTCTGGAAATGCGTTTAACGTAATTCCAGAAACGGCGGAATTAAAAGGTACAGTTAGAACCTATTCTAAAAAGATGTT
TGAAGAAGTTCCAGGAAAATTAGAAAGAGTGGTAAAAGGAATAGCTTCTGCATTAGGTGCCACTGTATCTATTCGTTACG
AAAGAACGAATCAGCCTACAATCAACGATCCTAAAATGGCAAACATAGTACGTAAAGCTTCTTTGAACATTCTTGGCGAA
GGAAGTTTAACAGAAGAAAACACAAAGTCCATGGGAGGAGAAGATTTCTCCGCGTTTCTGATGAAAGTTCCAGGTTGTTA
TTTTTTCGTAGGTTCTAGAAACGAAGAAAAGGGATTCGTTTATCCACACCATAGTTCTAAATTCGACATAGACGAAGATT
CTCTCTCAATCGGTCTCAGCGTTCTTAAAGAAGCGATTAAGATTTATTTAGAAGAAAATTGA

Upstream 100 bases:

>100_bases
CAGTTTTAGCGTAAAATACCAAGATTTTATTTTATAGTTTCAAATAAAAAAATCTAAAATCCTTTTCGAAAACGGTTCTC
AAAAGTAGAATGGTTTTAGT

Downstream 100 bases:

>100_bases
TTTCTGCTAAATAATCATAATAAATTCATTAAATGCATGTTTGTTTGTAAAAATTATTTCGAACTTTTTGTGTTCAAAAA
TTTTTATCAATCTTACACAA

Product: N-acyl-L-amino acid amidohydrolase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKHTLTSNRMAELIRYRRQIHKHPELRYEENQTSDYVINHLKELGLSFQDKIAKTGVVSLIDSGKPGKTLLVRADMDALP
IFEESNQEYKSVHEGVMHACGHDAHTSILMGLATEIKENIQFILPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNVDAA
LALHVWNHIPIGKIGVVDGAMMAAVDEFTITISGISGHGAMPQHTVDPIVVGAQIVNSLQTIVSRNTDPLDSCVVTVGSF
HSGNAFNVIPETAELKGTVRTYSKKMFEEVPGKLERVVKGIASALGATVSIRYERTNQPTINDPKMANIVRKASLNILGE
GSLTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYLEEN

Sequences:

>Translated_393_residues
MKHTLTSNRMAELIRYRRQIHKHPELRYEENQTSDYVINHLKELGLSFQDKIAKTGVVSLIDSGKPGKTLLVRADMDALP
IFEESNQEYKSVHEGVMHACGHDAHTSILMGLATEIKENIQFILPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNVDAA
LALHVWNHIPIGKIGVVDGAMMAAVDEFTITISGISGHGAMPQHTVDPIVVGAQIVNSLQTIVSRNTDPLDSCVVTVGSF
HSGNAFNVIPETAELKGTVRTYSKKMFEEVPGKLERVVKGIASALGATVSIRYERTNQPTINDPKMANIVRKASLNILGE
GSLTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYLEEN
>Mature_393_residues
MKHTLTSNRMAELIRYRRQIHKHPELRYEENQTSDYVINHLKELGLSFQDKIAKTGVVSLIDSGKPGKTLLVRADMDALP
IFEESNQEYKSVHEGVMHACGHDAHTSILMGLATEIKENIQFILPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNVDAA
LALHVWNHIPIGKIGVVDGAMMAAVDEFTITISGISGHGAMPQHTVDPIVVGAQIVNSLQTIVSRNTDPLDSCVVTVGSF
HSGNAFNVIPETAELKGTVRTYSKKMFEEVPGKLERVVKGIASALGATVSIRYERTNQPTINDPKMANIVRKASLNILGE
GSLTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYLEEN

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=26.7139479905437, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787598, Length=304, Percent_Identity=22.6973684210526, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 43221; Mature: 43221

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTLTSNRMAELIRYRRQIHKHPELRYEENQTSDYVINHLKELGLSFQDKIAKTGVVSL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
IDSGKPGKTLLVRADMDALPIFEESNQEYKSVHEGVMHACGHDAHTSILMGLATEIKENI
HCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
QFILPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNVDAALALHVWNHIPIGKIGVVDGA
EEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCEEHHCCH
MMAAVDEFTITISGISGHGAMPQHTVDPIVVGAQIVNSLQTIVSRNTDPLDSCVVTVGSF
HHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
HSGNAFNVIPETAELKGTVRTYSKKMFEEVPGKLERVVKGIASALGATVSIRYERTNQPT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCC
INDPKMANIVRKASLNILGEGSLTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFV
CCCCHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCEE
YPHHSSKFDIDEDSLSIGLSVLKEAIKIYLEEN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKHTLTSNRMAELIRYRRQIHKHPELRYEENQTSDYVINHLKELGLSFQDKIAKTGVVSL
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
IDSGKPGKTLLVRADMDALPIFEESNQEYKSVHEGVMHACGHDAHTSILMGLATEIKENI
HCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
QFILPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNVDAALALHVWNHIPIGKIGVVDGA
EEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCEEHHCCH
MMAAVDEFTITISGISGHGAMPQHTVDPIVVGAQIVNSLQTIVSRNTDPLDSCVVTVGSF
HHHHHEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
HSGNAFNVIPETAELKGTVRTYSKKMFEEVPGKLERVVKGIASALGATVSIRYERTNQPT
CCCCEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEECCCCCC
INDPKMANIVRKASLNILGEGSLTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFV
CCCCHHHHHHHHHHCCEEECCCCCCCHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCEE
YPHHSSKFDIDEDSLSIGLSVLKEAIKIYLEEN
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]