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The map label for this gene is rlmL [H]

Identifier: 45657189

GI number: 45657189

Start: 1616487

End: 1617737

Strand: Reverse

Name: rlmL [H]

Synonym: LIC11309

Alternate gene names: 45657189

Gene position: 1617737-1616487 (Counterclockwise)

Preceding gene: 45657190

Following gene: 45657188

Centisome position: 37.82

GC content: 36.53

Gene sequence:

>1251_bases
GTGACAAATTTTAAAAAACCAATTCCTAAAAAATCCCTCCGGTTAAAATCAAAAAGATCTGATAGAAAAAATTTTTCGAA
CGATCCAAGGCAAAAACAAAATCCTTTAAAATCGGAATGGGATTTTTCAAAACCGGATTCTTTTGAATATCACGCTTCTT
GTCCAGATGGTCTTTCTGGGCTGTTGCGAGAAGAAGTAATAGAAGCCGGTTTAAAGATCATCTCAGAAAACAGAGGTGGA
ATTTTTTTTCAAGGTCCAGCAAAAGCCTTAAAAGAATTTATTCTTTCTACTGGAATCGCGTCCGGAATCAGCATTTCGTT
AAAATATTGGAGAGTAGAAAACCCGGAAGATCTTTACGATCAAGCACTTCAATTTCCTTTTGAGAAAATTTTAAATTCAG
AACATTCTTTGAGAATCGATTCTGCTACTAAAGATTCCTTAAAAGATTCACGTTATGCGACATACAAACTGAAAGATGCA
ATCTTTGACCGTTTTCGCTCTAAGGGAAAAGAACCTCCAAAAATTTCAAGGGAAGAACCAGATTTTTTATTCTATCTACG
TTCTCATTCTGATCACGCAAAACTTTCTTTAGGTTTAAACACAAGACCTCTTCAACAGAGAGGACACGGTAGAATTGGAG
GGGAAGCACCCATTCGAGAAATTCTAGCTTCCGCTTTGATTCGTTATTCCGGATGGGACGCCAAATCACAGTTATACGAC
CCATTCTGTGGTTCAGGAACAGTTGTAGTAGAGGCTGCGCTCAAACTTCTATATGGAGGCCATACCAATTATAGAAGTTT
AGCGTCTTCTCTTCCGTTTCAAAAACTTTTCGGACAGCCAAACTTTAAAGAGAATTTAAATCTTTCTTCGGAAATAAAAA
TATTTGCTTCCGATTTAGATCCAAAGACTCTTGGGTTGGCTAGGTTAAACGCAAAAAATGCGGGAGTGGATCATTTGATT
CATTTTTTCGAATCAGATGCAAAGATTTCCGAAAACGAGGAAAAAATTTCCGAAGGTTTTATTGTAACAAATCCTCCTTA
CGGGGTTCGTTTAGGAACTAAAGAAGAAGCCAAAGAAGCTTACTTGGCTTGGGGTAAAAAACTAAAAGATCACTTTTCGG
GAAATGTTCTTGCGATCGTCTGTGGAGATACTTCTCTTTTAGGTTTTCTAAAATTAAAAAAAGACAAAGAACAATCTCTA
ACGATTGGAAAGTTAAAAGGAAAATTAGTTGCCTACACTTTAGGTAGATAA

Upstream 100 bases:

>100_bases
AGATTTTGATCTCGGAAGAAGAACATATCGATTGGATAGAATCTCAACAAGAAATCATTCGTCAAATCGGCGTTGAAAAT
TATCTTGCACAACAAATAGA

Downstream 100 bases:

>100_bases
TCCAATCGATTCGGAATGAATCATCAAGAAATTCTACTAAAACTTTTAGAAGTTACTGAGAATACTCAAGATTCGTTTCA
GTTTTTAAAACGATTTCGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTNFKKPIPKKSLRLKSKRSDRKNFSNDPRQKQNPLKSEWDFSKPDSFEYHASCPDGLSGLLREEVIEAGLKIISENRGG
IFFQGPAKALKEFILSTGIASGISISLKYWRVENPEDLYDQALQFPFEKILNSEHSLRIDSATKDSLKDSRYATYKLKDA
IFDRFRSKGKEPPKISREEPDFLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGEAPIREILASALIRYSGWDAKSQLYD
PFCGSGTVVVEAALKLLYGGHTNYRSLASSLPFQKLFGQPNFKENLNLSSEIKIFASDLDPKTLGLARLNAKNAGVDHLI
HFFESDAKISENEEKISEGFIVTNPPYGVRLGTKEEAKEAYLAWGKKLKDHFSGNVLAIVCGDTSLLGFLKLKKDKEQSL
TIGKLKGKLVAYTLGR

Sequences:

>Translated_416_residues
MTNFKKPIPKKSLRLKSKRSDRKNFSNDPRQKQNPLKSEWDFSKPDSFEYHASCPDGLSGLLREEVIEAGLKIISENRGG
IFFQGPAKALKEFILSTGIASGISISLKYWRVENPEDLYDQALQFPFEKILNSEHSLRIDSATKDSLKDSRYATYKLKDA
IFDRFRSKGKEPPKISREEPDFLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGEAPIREILASALIRYSGWDAKSQLYD
PFCGSGTVVVEAALKLLYGGHTNYRSLASSLPFQKLFGQPNFKENLNLSSEIKIFASDLDPKTLGLARLNAKNAGVDHLI
HFFESDAKISENEEKISEGFIVTNPPYGVRLGTKEEAKEAYLAWGKKLKDHFSGNVLAIVCGDTSLLGFLKLKKDKEQSL
TIGKLKGKLVAYTLGR
>Mature_415_residues
TNFKKPIPKKSLRLKSKRSDRKNFSNDPRQKQNPLKSEWDFSKPDSFEYHASCPDGLSGLLREEVIEAGLKIISENRGGI
FFQGPAKALKEFILSTGIASGISISLKYWRVENPEDLYDQALQFPFEKILNSEHSLRIDSATKDSLKDSRYATYKLKDAI
FDRFRSKGKEPPKISREEPDFLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGEAPIREILASALIRYSGWDAKSQLYDP
FCGSGTVVVEAALKLLYGGHTNYRSLASSLPFQKLFGQPNFKENLNLSSEIKIFASDLDPKTLGLARLNAKNAGVDHLIH
FFESDAKISENEEKISEGFIVTNPPYGVRLGTKEEAKEAYLAWGKKLKDHFSGNVLAIVCGDTSLLGFLKLKKDKEQSLT
IGKLKGKLVAYTLGR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=339, Percent_Identity=29.2035398230088, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 46694; Mature: 46563

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNFKKPIPKKSLRLKSKRSDRKNFSNDPRQKQNPLKSEWDFSKPDSFEYHASCPDGLSG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCCHHHHH
LLREEVIEAGLKIISENRGGIFFQGPAKALKEFILSTGIASGISISLKYWRVENPEDLYD
HHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
QALQFPFEKILNSEHSLRIDSATKDSLKDSRYATYKLKDAIFDRFRSKGKEPPKISREEP
HHHHCHHHHHCCCCCCEEECCCCHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
DFLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGEAPIREILASALIRYSGWDAKSQLYD
CEEEEEECCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCC
PFCGSGTVVVEAALKLLYGGHTNYRSLASSLPFQKLFGQPNFKENLNLSSEIKIFASDLD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCHHCCCCCCCEEEEECCCC
PKTLGLARLNAKNAGVDHLIHFFESDAKISENEEKISEGFIVTNPPYGVRLGTKEEAKEA
CCHHHHEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHCCEEEECCCCCEEECCHHHHHHH
YLAWGKKLKDHFSGNVLAIVCGDTSLLGFLKLKKDKEQSLTIGKLKGKLVAYTLGR
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHCCCHHHHCCEEEEEECCC
>Mature Secondary Structure 
TNFKKPIPKKSLRLKSKRSDRKNFSNDPRQKQNPLKSEWDFSKPDSFEYHASCPDGLSG
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCEECCCCHHHHH
LLREEVIEAGLKIISENRGGIFFQGPAKALKEFILSTGIASGISISLKYWRVENPEDLYD
HHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHH
QALQFPFEKILNSEHSLRIDSATKDSLKDSRYATYKLKDAIFDRFRSKGKEPPKISREEP
HHHHCHHHHHCCCCCCEEECCCCHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCC
DFLFYLRSHSDHAKLSLGLNTRPLQQRGHGRIGGEAPIREILASALIRYSGWDAKSQLYD
CEEEEEECCCCCCEEEECCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHCC
PFCGSGTVVVEAALKLLYGGHTNYRSLASSLPFQKLFGQPNFKENLNLSSEIKIFASDLD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHCCCCCCHHCCCCCCCEEEEECCCC
PKTLGLARLNAKNAGVDHLIHFFESDAKISENEEKISEGFIVTNPPYGVRLGTKEEAKEA
CCHHHHEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHCCEEEECCCCCEEECCHHHHHHH
YLAWGKKLKDHFSGNVLAIVCGDTSLLGFLKLKKDKEQSLTIGKLKGKLVAYTLGR
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCHHCCCHHHHCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA