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The map label for this gene is nadE

Identifier: 45656959

GI number: 45656959

Start: 1327258

End: 1329186

Strand: Reverse

Name: nadE

Synonym: LIC11075

Alternate gene names: 45656959

Gene position: 1329186-1327258 (Counterclockwise)

Preceding gene: 45656962

Following gene: 45656957

Centisome position: 31.08

GC content: 37.33

Gene sequence:

>1929_bases
ATGCAGTCCGTAAGACTTACGTCCGTTTCTCTTAAAACGAAAGTATTCGATTTTCAAGGAAACTTAGAAAAAATCAAAAG
AGTTCTGAAATTAGAGAAAAAATCGGATCTGATTTTATTCCCAGAACTTTGTATCTCCGGCTACGGATGTGAAGACGCAT
TTTACTTTCCAAGAATTTGGAAAAAATCCTGGAATTCTTTAATACAACTACTTCCTCATACGGAAAATAAAATCATAGTA
GTTGGCTTACCTATATTCCAAAATCCTTATTTATTTAACTGTGCTGCGGTACTTTGTAACGGCGTCGTAGCGGGAATTGT
ACCTAAATCCAATTTAGCTTCCACAGGAGTTCATTATGAAAACAGATGGTTCACTAGAGGCGAAGAATCTCAAGAAAACT
TTATAGCACCCGACGGATCAACGATTCCTTTCGGTTCTCTCATTTTTGAAACAGATCATTTTTCTTTCGGCGTAGAAATC
TGCGAAGACTCGTGGGTATTACAAAAACCTTCTATACCACTTGCAGAAGCAGGAACAGATCTAATCCTTTCTCCGGGAGC
GTCTCATTTTGCATTTGGAAAACAAAGAATTCGTAGACAAATCTTCCAAGAAAGTTCTAGAAGAGAGTCTAACGTATATC
TATTTTCTAATTTATGCGGAAACGAATCCGGAAGGCTGATTTTTGAAGGTGGTTCCATGATCGTTCAAAATGGGAAATTA
CTTTCAGAATCGGAAAGACTCTTTTTCGGAGATTTTAGTCTTTGCTCCAACGAGATCGACTTCGAAGCGTCCCAAGCAGA
TAGAGCAAAAAATTTTCGTCCTTCCGGAAATCGTTTTAGTAAAACAAAATCAACCGAAGAAAATAAAATCTATTTGGGTA
TAGAATTTCCCAAAAGAGCCCCAAAGGTAAACAAATCCTTACTGGAAATTTCAGTTTCCAAAGAAGAGGAATCATATCTG
GATTTTACGAAAGCAGTGGCATTAGGACTTTTTGATTATCTAATGTACTCTAATACAAAAGGTTATACACTTTCTCTTTC
CGGGGGGGCCGACAGTTCCGCGTGCGCCTTACTTGTAACCGCAATGAAAAAAATCGCAAAATTAGAATTAGGTGAGAACT
TTTTTAAATCCAAAGGAATTCCAGAAGAGAGTATACTTTGTACACTTTATCAGTCTACTTCAAACAACTCAGATCGGACT
AAATTTTTAGCCAAATCATTAGCAAAAGAAATTCAATCAATCCACGGGGATTTAACAATCGATTCGGAAATACAAAGTAT
CTCCGAAAAAATCTCCAAACTTACCGGAATTTCCTTGCGTTGGGATAAACATAACTTAACTCTACAAAACATTCAAGCGA
GAGTTCGTTCTCCCATTATATGGATGCTCGCCAATTTAAACGGACATCTTCTTCTTTCCACCGGAAATAGAAGCGAAGCA
AGCGTAGGTTATACGACGATGGATGGAGATTCTTCCGGATCAATCGCACCGTTAACAGGAGTCAGCAAAGAATTTATTTT
GAAATGGTTACGTTTTGTTTCAGAAGGAAAAGACCCAATTCTTCCGGCATATCCTTCCATCAAAGAAATTGTAAATTCTC
CACCCAGCGCCGAATTAAAACCACCAGAAGATAAACAAGAAGATGAAAAAGATCTGATGCCCTATCCACTCTTGCAAAAA
ATAGAAGAACTATTTATAGTAAGAGGAGCTGGTTATTCCGAAATTATACAACTTCTTTCCGAAGATTCTGAAATACAAAA
CCTACCTCCCAGATTTTTAGAAGAATCGGTAAAGAAATACATTTCACTGTTTCATAAAAATCAGTGGAAACGAGAAAGGC
TTCCACCTTCATTTCATTTAGACGATTATGGGCTGGACCCAAAATCTAGCTTTCGATTCCCAATTCTTTCCGAGGATATC
GAATTATAA

Upstream 100 bases:

>100_bases
AGCATTTTTTAACCAGTGAATTTCCAGGAAAGAATCCGGAAACCCGTTTTCAAAATAAAAAAAACTTTCAAAACGGATTC
CGACATGAATCCTGTAATTC

Downstream 100 bases:

>100_bases
ATTTAGAATATTTTTAATCTATAATATTTAACAAAAACACATTAAGAAATTATTATAAATCCTTAAAACAATGTGTTTTA
TTATAAAACGTCATAAATTC

Product: NH(3)-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MQSVRLTSVSLKTKVFDFQGNLEKIKRVLKLEKKSDLILFPELCISGYGCEDAFYFPRIWKKSWNSLIQLLPHTENKIIV
VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEESQENFIAPDGSTIPFGSLIFETDHFSFGVEI
CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRQIFQESSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL
LSESERLFFGDFSLCSNEIDFEASQADRAKNFRPSGNRFSKTKSTEENKIYLGIEFPKRAPKVNKSLLEISVSKEEESYL
DFTKAVALGLFDYLMYSNTKGYTLSLSGGADSSACALLVTAMKKIAKLELGENFFKSKGIPEESILCTLYQSTSNNSDRT
KFLAKSLAKEIQSIHGDLTIDSEIQSISEKISKLTGISLRWDKHNLTLQNIQARVRSPIIWMLANLNGHLLLSTGNRSEA
SVGYTTMDGDSSGSIAPLTGVSKEFILKWLRFVSEGKDPILPAYPSIKEIVNSPPSAELKPPEDKQEDEKDLMPYPLLQK
IEELFIVRGAGYSEIIQLLSEDSEIQNLPPRFLEESVKKYISLFHKNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEDI
EL

Sequences:

>Translated_642_residues
MQSVRLTSVSLKTKVFDFQGNLEKIKRVLKLEKKSDLILFPELCISGYGCEDAFYFPRIWKKSWNSLIQLLPHTENKIIV
VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEESQENFIAPDGSTIPFGSLIFETDHFSFGVEI
CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRQIFQESSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL
LSESERLFFGDFSLCSNEIDFEASQADRAKNFRPSGNRFSKTKSTEENKIYLGIEFPKRAPKVNKSLLEISVSKEEESYL
DFTKAVALGLFDYLMYSNTKGYTLSLSGGADSSACALLVTAMKKIAKLELGENFFKSKGIPEESILCTLYQSTSNNSDRT
KFLAKSLAKEIQSIHGDLTIDSEIQSISEKISKLTGISLRWDKHNLTLQNIQARVRSPIIWMLANLNGHLLLSTGNRSEA
SVGYTTMDGDSSGSIAPLTGVSKEFILKWLRFVSEGKDPILPAYPSIKEIVNSPPSAELKPPEDKQEDEKDLMPYPLLQK
IEELFIVRGAGYSEIIQLLSEDSEIQNLPPRFLEESVKKYISLFHKNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEDI
EL
>Mature_642_residues
MQSVRLTSVSLKTKVFDFQGNLEKIKRVLKLEKKSDLILFPELCISGYGCEDAFYFPRIWKKSWNSLIQLLPHTENKIIV
VGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYENRWFTRGEESQENFIAPDGSTIPFGSLIFETDHFSFGVEI
CEDSWVLQKPSIPLAEAGTDLILSPGASHFAFGKQRIRRQIFQESSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL
LSESERLFFGDFSLCSNEIDFEASQADRAKNFRPSGNRFSKTKSTEENKIYLGIEFPKRAPKVNKSLLEISVSKEEESYL
DFTKAVALGLFDYLMYSNTKGYTLSLSGGADSSACALLVTAMKKIAKLELGENFFKSKGIPEESILCTLYQSTSNNSDRT
KFLAKSLAKEIQSIHGDLTIDSEIQSISEKISKLTGISLRWDKHNLTLQNIQARVRSPIIWMLANLNGHLLLSTGNRSEA
SVGYTTMDGDSSGSIAPLTGVSKEFILKWLRFVSEGKDPILPAYPSIKEIVNSPPSAELKPPEDKQEDEKDLMPYPLLQK
IEELFIVRGAGYSEIIQLLSEDSEIQNLPPRFLEESVKKYISLFHKNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEDI
EL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=675, Percent_Identity=28.4444444444444, Blast_Score=241, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17538756, Length=694, Percent_Identity=29.2507204610951, Blast_Score=253, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321865, Length=678, Percent_Identity=27.8761061946903, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24641841, Length=683, Percent_Identity=28.696925329429, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24641843, Length=683, Percent_Identity=28.696925329429, Blast_Score=249, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 72193; Mature: 72193

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSVRLTSVSLKTKVFDFQGNLEKIKRVLKLEKKSDLILFPELCISGYGCEDAFYFPRIW
CCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCHHHHHHH
KKSWNSLIQLLPHTENKIIVVGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYE
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC
NRWFTRGEESQENFIAPDGSTIPFGSLIFETDHFSFGVEICEDSWVLQKPSIPLAEAGTD
CCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCEECEEEECCCEEEECCCCCHHHCCCC
LILSPGASHFAFGKQRIRRQIFQESSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEECCCE
LSESERLFFGDFSLCSNEIDFEASQADRAKNFRPSGNRFSKTKSTEENKIYLGIEFPKRA
ECCCCCEEEECHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
PKVNKSLLEISVSKEEESYLDFTKAVALGLFDYLMYSNTKGYTLSLSGGADSSACALLVT
CCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AMKKIAKLELGENFFKSKGIPEESILCTLYQSTSNNSDRTKFLAKSLAKEIQSIHGDLTI
HHHHHHHHHHCCHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEE
DSEIQSISEKISKLTGISLRWDKHNLTLQNIQARVRSPIIWMLANLNGHLLLSTGNRSEA
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC
SVGYTTMDGDSSGSIAPLTGVSKEFILKWLRFVSEGKDPILPAYPSIKEIVNSPPSAELK
CCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
PPEDKQEDEKDLMPYPLLQKIEELFIVRGAGYSEIIQLLSEDSEIQNLPPRFLEESVKKY
CCCCCCCCHHCCCCCHHHHHHHHHHEECCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
ISLFHKNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEDIEL
HHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQSVRLTSVSLKTKVFDFQGNLEKIKRVLKLEKKSDLILFPELCISGYGCEDAFYFPRIW
CCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCHHHHHHH
KKSWNSLIQLLPHTENKIIVVGLPIFQNPYLFNCAAVLCNGVVAGIVPKSNLASTGVHYE
HHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC
NRWFTRGEESQENFIAPDGSTIPFGSLIFETDHFSFGVEICEDSWVLQKPSIPLAEAGTD
CCCCCCCCCCCCCEECCCCCCCCCCCEEEECCCCEECEEEECCCEEEECCCCCHHHCCCC
LILSPGASHFAFGKQRIRRQIFQESSRRESNVYLFSNLCGNESGRLIFEGGSMIVQNGKL
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEECCCE
LSESERLFFGDFSLCSNEIDFEASQADRAKNFRPSGNRFSKTKSTEENKIYLGIEFPKRA
ECCCCCEEEECHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
PKVNKSLLEISVSKEEESYLDFTKAVALGLFDYLMYSNTKGYTLSLSGGADSSACALLVT
CCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
AMKKIAKLELGENFFKSKGIPEESILCTLYQSTSNNSDRTKFLAKSLAKEIQSIHGDLTI
HHHHHHHHHHCCHHHHHCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEE
DSEIQSISEKISKLTGISLRWDKHNLTLQNIQARVRSPIIWMLANLNGHLLLSTGNRSEA
HHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCC
SVGYTTMDGDSSGSIAPLTGVSKEFILKWLRFVSEGKDPILPAYPSIKEIVNSPPSAELK
CCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
PPEDKQEDEKDLMPYPLLQKIEELFIVRGAGYSEIIQLLSEDSEIQNLPPRFLEESVKKY
CCCCCCCCHHCCCCCHHHHHHHHHHEECCCCHHHHHHHHCCCCHHHCCCHHHHHHHHHHH
ISLFHKNQWKRERLPPSFHLDDYGLDPKSSFRFPILSEDIEL
HHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]