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The map label for this gene is trpE

Identifier: 45656480

GI number: 45656480

Start: 707696

End: 709117

Strand: Reverse

Name: trpE

Synonym: LIC10582

Alternate gene names: 45656480

Gene position: 709117-707696 (Counterclockwise)

Preceding gene: 45656481

Following gene: 45656479

Centisome position: 16.58

GC content: 39.73

Gene sequence:

>1422_bases
ATGGAAACTCCAGTTTCTCTGTTTGCAAAATGGGGATGTGAATCTTCTCAAAATTCCTTCCTACTCGAATCTGTAGAAGG
CGGAGAAAAACTGGGAAGAAATTCTTTTTTAGGTAAAACTCCTTCTCGTCTCCTTCAAGGTAAGGACGGTCTTTTTTATA
TCACAGTTGCAAATGAACCTCCTACAGAAGTAATCACTTATGATCCTTTGGTTCTTTTAGAAAATCTTTTGGGAGATGAC
GTATATGTTCAAGATTATCGTCTTCCTCCTTTTGCAGGTGGAGCGGTCGGTTTTTTATCCTTTGCCGCGGTTCGTTATTA
CGAAAATATTCCAGATACAAAACCAGAAGATGAAAATGCCCCCGACTGCTATTTTGGAATTTATGATGAACTTCTAATAG
TCGATCATATTGATCATGTGTTAAGAATTGTTGTCAATGCAAGAGTCGGAGAACACTCCTCCCTGAGAGAATGTTACGAT
TTTGTAATTGATAAAATCAACTCTATCGAAAACGAAATTCGAAACGGAAGTATCCCGGAAGAGATCAAAAATCCTAAAAT
AGCAACCGGAAGTCTAGAGTTAAATCCGAATATTCCAGATGAAGAATATAAGAAAAATGTAGAAAAAGCCAAAGAATATA
TTCAAGCAGGAGATATTTTTCAGGTAGTTCCTTCCCGTAAGTTGAAATTTAAGCCGGATGTTCCTCCGTTTCAAGTCTAT
CGTGGCTTAAGAACCGTAAATCCCAGTCCCTACATGTATTATCTTAAGTTAGGCGAAATTACCATCGTCGGAAGTTCTCC
CGAAATTATGGTAAAATACGCTGGAAATCAGACCTATCTTCGTCCGATCGCCGGAACCCGTCCACGAGGCAAAAATCCAA
ATGAAGATAAGTTTCTAGAAGAGAATCTTCTTTCTGATCCTAAAGAAATTTCCGAACATATTATGTTAGTCGACTTAGGA
CGTAACGATCTAGGAAGAGTTTGTAAACCTGGAAGCGTTCACGTGAATGATTTCAAAGTTATTGAAAAATATTCCCACGT
TATGCACATTGTAAGTCAGTGTTCCGGCGAACTGGACGATGAAAAAACTGTTTACGATTTGATTCGCGCCGCTCTTCCCG
CAGGGACCGTGTCGGGTGCGCCTAAAATCCGCGCGATGGAGATTATAGACGAATTAGAAACAACTCGAAGAGCGATTTAT
TCTGGTGCTTTGGGCTATATTTCTTTTTCGGGAGAAACTGATCTTGCAATCATCATTCGTACGATCGTGTTTTACAAAGA
CACGGCGTTCTTACAGGCAGGCGGAGGAGTTGTATACGATTCGGTTCCAGAGTTGGAATTAGAAGAAACGAAAAATAAAA
TGGCAGCGCTTTTAAAAGCGGTAGAGTTCGCAAGAAATGGACTCAAAGGAGAATGGAAATAA

Upstream 100 bases:

>100_bases
GGCCGAACTTCTGAAAGAATTCTCACTCACATTCGAAGAATTTTCCGTTCAAGCAGAACGTGGAAATTTAATTCCTATCT
TCAAACAGATCTTTTTAGAT

Downstream 100 bases:

>100_bases
TGTTTCTCCTGATCGATAATTACGATTCTTTCACATACAATCTTTATCAATATTTTTCTCAGATAGGAAATGAAATCGAA
GTTTATCGTAACGATAAAAT

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
METPVSLFAKWGCESSQNSFLLESVEGGEKLGRNSFLGKTPSRLLQGKDGLFYITVANEPPTEVITYDPLVLLENLLGDD
VYVQDYRLPPFAGGAVGFLSFAAVRYYENIPDTKPEDENAPDCYFGIYDELLIVDHIDHVLRIVVNARVGEHSSLRECYD
FVIDKINSIENEIRNGSIPEEIKNPKIATGSLELNPNIPDEEYKKNVEKAKEYIQAGDIFQVVPSRKLKFKPDVPPFQVY
RGLRTVNPSPYMYYLKLGEITIVGSSPEIMVKYAGNQTYLRPIAGTRPRGKNPNEDKFLEENLLSDPKEISEHIMLVDLG
RNDLGRVCKPGSVHVNDFKVIEKYSHVMHIVSQCSGELDDEKTVYDLIRAALPAGTVSGAPKIRAMEIIDELETTRRAIY
SGALGYISFSGETDLAIIIRTIVFYKDTAFLQAGGGVVYDSVPELELEETKNKMAALLKAVEFARNGLKGEWK

Sequences:

>Translated_473_residues
METPVSLFAKWGCESSQNSFLLESVEGGEKLGRNSFLGKTPSRLLQGKDGLFYITVANEPPTEVITYDPLVLLENLLGDD
VYVQDYRLPPFAGGAVGFLSFAAVRYYENIPDTKPEDENAPDCYFGIYDELLIVDHIDHVLRIVVNARVGEHSSLRECYD
FVIDKINSIENEIRNGSIPEEIKNPKIATGSLELNPNIPDEEYKKNVEKAKEYIQAGDIFQVVPSRKLKFKPDVPPFQVY
RGLRTVNPSPYMYYLKLGEITIVGSSPEIMVKYAGNQTYLRPIAGTRPRGKNPNEDKFLEENLLSDPKEISEHIMLVDLG
RNDLGRVCKPGSVHVNDFKVIEKYSHVMHIVSQCSGELDDEKTVYDLIRAALPAGTVSGAPKIRAMEIIDELETTRRAIY
SGALGYISFSGETDLAIIIRTIVFYKDTAFLQAGGGVVYDSVPELELEETKNKMAALLKAVEFARNGLKGEWK
>Mature_473_residues
METPVSLFAKWGCESSQNSFLLESVEGGEKLGRNSFLGKTPSRLLQGKDGLFYITVANEPPTEVITYDPLVLLENLLGDD
VYVQDYRLPPFAGGAVGFLSFAAVRYYENIPDTKPEDENAPDCYFGIYDELLIVDHIDHVLRIVVNARVGEHSSLRECYD
FVIDKINSIENEIRNGSIPEEIKNPKIATGSLELNPNIPDEEYKKNVEKAKEYIQAGDIFQVVPSRKLKFKPDVPPFQVY
RGLRTVNPSPYMYYLKLGEITIVGSSPEIMVKYAGNQTYLRPIAGTRPRGKNPNEDKFLEENLLSDPKEISEHIMLVDLG
RNDLGRVCKPGSVHVNDFKVIEKYSHVMHIVSQCSGELDDEKTVYDLIRAALPAGTVSGAPKIRAMEIIDELETTRRAIY
SGALGYISFSGETDLAIIIRTIVFYKDTAFLQAGGGVVYDSVPELELEETKNKMAALLKAVEFARNGLKGEWK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=409, Percent_Identity=37.6528117359413, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788114, Length=410, Percent_Identity=34.390243902439, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI87082077, Length=272, Percent_Identity=25, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786809, Length=265, Percent_Identity=23.3962264150943, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=457, Percent_Identity=39.8249452954048, Blast_Score=303, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6324361, Length=411, Percent_Identity=25.7907542579075, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 52905; Mature: 52905

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPVSLFAKWGCESSQNSFLLESVEGGEKLGRNSFLGKTPSRLLQGKDGLFYITVANEP
CCCHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCC
PTEVITYDPLVLLENLLGDDVYVQDYRLPPFAGGAVGFLSFAAVRYYENIPDTKPEDENA
CCCEEECCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCYFGIYDELLIVDHIDHVLRIVVNARVGEHSSLRECYDFVIDKINSIENEIRNGSIPE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EIKNPKIATGSLELNPNIPDEEYKKNVEKAKEYIQAGDIFQVVPSRKLKFKPDVPPFQVY
HHCCCCEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEECCCCCCHHHHH
RGLRTVNPSPYMYYLKLGEITIVGSSPEIMVKYAGNQTYLRPIAGTRPRGKNPNEDKFLE
HHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHH
ENLLSDPKEISEHIMLVDLGRNDLGRVCKPGSVHVNDFKVIEKYSHVMHIVSQCSGELDD
HHHCCCHHHHHCCEEEEECCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCC
EKTVYDLIRAALPAGTVSGAPKIRAMEIIDELETTRRAIYSGALGYISFSGETDLAIIIR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
TIVFYKDTAFLQAGGGVVYDSVPELELEETKNKMAALLKAVEFARNGLKGEWK
HHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
METPVSLFAKWGCESSQNSFLLESVEGGEKLGRNSFLGKTPSRLLQGKDGLFYITVANEP
CCCHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCC
PTEVITYDPLVLLENLLGDDVYVQDYRLPPFAGGAVGFLSFAAVRYYENIPDTKPEDENA
CCCEEECCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCYFGIYDELLIVDHIDHVLRIVVNARVGEHSSLRECYDFVIDKINSIENEIRNGSIPE
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EIKNPKIATGSLELNPNIPDEEYKKNVEKAKEYIQAGDIFQVVPSRKLKFKPDVPPFQVY
HHCCCCEEECEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEECCCCCCHHHHH
RGLRTVNPSPYMYYLKLGEITIVGSSPEIMVKYAGNQTYLRPIAGTRPRGKNPNEDKFLE
HHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHH
ENLLSDPKEISEHIMLVDLGRNDLGRVCKPGSVHVNDFKVIEKYSHVMHIVSQCSGELDD
HHHCCCHHHHHCCEEEEECCCCCHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCCCC
EKTVYDLIRAALPAGTVSGAPKIRAMEIIDELETTRRAIYSGALGYISFSGETDLAIIIR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
TIVFYKDTAFLQAGGGVVYDSVPELELEETKNKMAALLKAVEFARNGLKGEWK
HHHHHCCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]