| Definition | Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence. |
|---|---|
| Accession | NC_005823 |
| Length | 4,277,185 |
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The map label for this gene is leuA [H]
Identifier: 45656309
GI number: 45656309
Start: 466921
End: 468207
Strand: Direct
Name: leuA [H]
Synonym: LIC10409
Alternate gene names: 45656309
Gene position: 466921-468207 (Clockwise)
Preceding gene: 45656306
Following gene: 45656312
Centisome position: 10.92
GC content: 39.86
Gene sequence:
>1287_bases ATGAAACAAGATTCACAATCGGAAAATGAGTCTATAGTGCGTGACCTTTCCGTTTCCGAAGATCAAAGAAATGTAAAGTT CTTCTCGGATCTTCAAACTCCAATTCCTAAACATCTTCCTTTTTTTATGGACGTGACTTTAAGAGACGGAAACCAAGCAC TTCGTAGACCTTGGAATTTAGAACAAAAAGAAACAATCTTTAAACAACTTTTAAAACTAGGAGTACAAGGAATTGAAGTA GGATTTGCTTCTTCTAACAACCAAGAATTTGAAGCCTGTAAATATCTTTCTTCGATTGCACCGGATAACGTAGTCATATC TTCTTTATCTAGAGCTGTGGAAAAAGAAATCGAAGTTTCTTGGAAGGCGATACGGTTTGCTCCAAAACCAAGGATTCACA TCGTATATCCTGTATCTGCGTTTACGATTCAAAACGTATTGAAGATAACTCCTGAAAAAGTTTTAGATAGGATTTCCCAG TCAGTGGCTTATGCAAAAAGTCTTGTAGGATCAAAAGGTGAAGTTCAATTTTCAGGAGAACATTTCGGAGATTCTTTGGA AAACTTAGATTTTGCTGCGGAAGCGTTTCAAATCGCTTTGAATAACGGAGCCGATGTTGTAAATCTTCCCAACACCGTCG AACGTTATCGTCCTTGGCTTTTTGTTTCTATGGTAAAGGCGGTTGCTAATTTATTGCCAGAGGATACAAGAATTTCGATT CATACACATAATGATCTTGGAATGGCGACTGCGACTACGGTAGAATCTTACTTTGCAGGAGCGGTTCAATTGGAGACGGC GTTAAACGGACTTGGAGAGAGGGCGGGAAATACAAACACATATGAAGTGGCAATTGCTCTTCATAACTGCGGAGTTGAAG TCCCTTTAAATTTTTCGACGATCTATGAAACATCTCGTTTGGTTTCTTATCTATCGGAGATTCCAATTTATGAAAAAGCG CCTTTGATTGGAGAAGACGTCATTTCTCATCGATCCGGAATTCATCAAGACGGAGTCGCAAAAACACGCCATCTACAAAA AGGCGCCTATCGCGCGTTTGACGCCGCGTTGATCGGAAGGCCGGAGGGGGACAGAATCGAATTTACAAATCAATCGGGTA AAAGTGCGGTCTATTGTATTTTAAAAGATGCAGGGGAAAACATTACCTTAGAAGAAGCTGGTAGGTTGCAGCCGATTTTA AAAAAGATTTCCGAAGATTTAGGTAGAAGAGAACTGACTTTGGAAGAAATTCGGATCGAATGGAATCGCTTGCTCAGAGC TATATAA
Upstream 100 bases:
>100_bases ATACACGAGCCTGCTCCTCCTGGAGTGGGCTTTTTTATTTCCGAGTTCATCTCTGTTCGGAAAATCCCTCTCCTGGACTG GCCGGTAAAAGGAGAAAACA
Downstream 100 bases:
>100_bases AGGATACCTAATGTTTTTTATTTAAATGGGATTTTGTAATGAAATCTAGTTCAACGTAAGAAAATTTGGACGAATCCGGC TGCAGTGCAGCCGGACCGGG
Product: 2-isopropylmalate synthase
Products: NA
Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]
Number of amino acids: Translated: 428; Mature: 428
Protein sequence:
>428_residues MKQDSQSENESIVRDLSVSEDQRNVKFFSDLQTPIPKHLPFFMDVTLRDGNQALRRPWNLEQKETIFKQLLKLGVQGIEV GFASSNNQEFEACKYLSSIAPDNVVISSLSRAVEKEIEVSWKAIRFAPKPRIHIVYPVSAFTIQNVLKITPEKVLDRISQ SVAYAKSLVGSKGEVQFSGEHFGDSLENLDFAAEAFQIALNNGADVVNLPNTVERYRPWLFVSMVKAVANLLPEDTRISI HTHNDLGMATATTVESYFAGAVQLETALNGLGERAGNTNTYEVAIALHNCGVEVPLNFSTIYETSRLVSYLSEIPIYEKA PLIGEDVISHRSGIHQDGVAKTRHLQKGAYRAFDAALIGRPEGDRIEFTNQSGKSAVYCILKDAGENITLEEAGRLQPIL KKISEDLGRRELTLEEIRIEWNRLLRAI
Sequences:
>Translated_428_residues MKQDSQSENESIVRDLSVSEDQRNVKFFSDLQTPIPKHLPFFMDVTLRDGNQALRRPWNLEQKETIFKQLLKLGVQGIEV GFASSNNQEFEACKYLSSIAPDNVVISSLSRAVEKEIEVSWKAIRFAPKPRIHIVYPVSAFTIQNVLKITPEKVLDRISQ SVAYAKSLVGSKGEVQFSGEHFGDSLENLDFAAEAFQIALNNGADVVNLPNTVERYRPWLFVSMVKAVANLLPEDTRISI HTHNDLGMATATTVESYFAGAVQLETALNGLGERAGNTNTYEVAIALHNCGVEVPLNFSTIYETSRLVSYLSEIPIYEKA PLIGEDVISHRSGIHQDGVAKTRHLQKGAYRAFDAALIGRPEGDRIEFTNQSGKSAVYCILKDAGENITLEEAGRLQPIL KKISEDLGRRELTLEEIRIEWNRLLRAI >Mature_428_residues MKQDSQSENESIVRDLSVSEDQRNVKFFSDLQTPIPKHLPFFMDVTLRDGNQALRRPWNLEQKETIFKQLLKLGVQGIEV GFASSNNQEFEACKYLSSIAPDNVVISSLSRAVEKEIEVSWKAIRFAPKPRIHIVYPVSAFTIQNVLKITPEKVLDRISQ SVAYAKSLVGSKGEVQFSGEHFGDSLENLDFAAEAFQIALNNGADVVNLPNTVERYRPWLFVSMVKAVANLLPEDTRISI HTHNDLGMATATTVESYFAGAVQLETALNGLGERAGNTNTYEVAIALHNCGVEVPLNFSTIYETSRLVSYLSEIPIYEKA PLIGEDVISHRSGIHQDGVAKTRHLQKGAYRAFDAALIGRPEGDRIEFTNQSGKSAVYCILKDAGENITLEEAGRLQPIL KKISEDLGRRELTLEEIRIEWNRLLRAI
Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]
COG id: COG0119
COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1786261, Length=352, Percent_Identity=33.2386363636364, Blast_Score=175, Evalue=4e-45, Organism=Saccharomyces cerevisiae, GI6324682, Length=404, Percent_Identity=29.9504950495049, Blast_Score=159, Evalue=1e-39, Organism=Saccharomyces cerevisiae, GI6324225, Length=404, Percent_Identity=29.4554455445545, Blast_Score=157, Evalue=2e-39,
Paralogues:
None
Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013709 - InterPro: IPR002034 - InterPro: IPR013785 - InterPro: IPR005671 - InterPro: IPR000891 [H]
Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]
EC number: =2.3.3.13 [H]
Molecular weight: Translated: 47789; Mature: 47789
Theoretical pI: Translated: 5.69; Mature: 5.69
Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 0.9 %Met (Translated Protein) 1.6 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKQDSQSENESIVRDLSVSEDQRNVKFFSDLQTPIPKHLPFFMDVTLRDGNQALRRPWNL CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCCCCC EQKETIFKQLLKLGVQGIEVGFASSNNQEFEACKYLSSIAPDNVVISSLSRAVEKEIEVS CHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH WKAIRFAPKPRIHIVYPVSAFTIQNVLKITPEKVLDRISQSVAYAKSLVGSKGEVQFSGE HHEEEECCCCCEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCH HFGDSLENLDFAAEAFQIALNNGADVVNLPNTVERYRPWLFVSMVKAVANLLPEDTRISI HHCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEE HTHNDLGMATATTVESYFAGAVQLETALNGLGERAGNTNTYEVAIALHNCGVEVPLNFST EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECCCHHH IYETSRLVSYLSEIPIYEKAPLIGEDVISHRSGIHQDGVAKTRHLQKGAYRAFDAALIGR HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC PEGDRIEFTNQSGKSAVYCILKDAGENITLEEAGRLQPILKKISEDLGRRELTLEEIRIE CCCCEEEEECCCCCEEEEEEEECCCCEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHH WNRLLRAI HHHHHHCC >Mature Secondary Structure MKQDSQSENESIVRDLSVSEDQRNVKFFSDLQTPIPKHLPFFMDVTLRDGNQALRRPWNL CCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEEECCCHHHCCCCCC EQKETIFKQLLKLGVQGIEVGFASSNNQEFEACKYLSSIAPDNVVISSLSRAVEKEIEVS CHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH WKAIRFAPKPRIHIVYPVSAFTIQNVLKITPEKVLDRISQSVAYAKSLVGSKGEVQFSGE HHEEEECCCCCEEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCH HFGDSLENLDFAAEAFQIALNNGADVVNLPNTVERYRPWLFVSMVKAVANLLPEDTRISI HHCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEE HTHNDLGMATATTVESYFAGAVQLETALNGLGERAGNTNTYEVAIALHNCGVEVPLNFST EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCEEECCCHHH IYETSRLVSYLSEIPIYEKAPLIGEDVISHRSGIHQDGVAKTRHLQKGAYRAFDAALIGR HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC PEGDRIEFTNQSGKSAVYCILKDAGENITLEEAGRLQPILKKISEDLGRRELTLEEIRIE CCCCEEEEECCCCCEEEEEEEECCCCEEEHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHH WNRLLRAI HHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA