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The map label for this gene is trpS

Identifier: 45656260

GI number: 45656260

Start: 404374

End: 405342

Strand: Reverse

Name: trpS

Synonym: LIC10357

Alternate gene names: 45656260

Gene position: 405342-404374 (Counterclockwise)

Preceding gene: 45656261

Following gene: 45656259

Centisome position: 9.48

GC content: 36.84

Gene sequence:

>969_bases
ATGAGGATTCTCACTGGGGTCCAACCTTCCGGTAAACTTCACTTAGGAAATTTTTTCTCAGTCATTCGAAAACTCAAAGA
ATACCAAGATTCGACTGACTTATTTTGTTTTATCGCAGATTTACATTCTCTAACTACGTTTTCTTCAAAAGAAAAACAAA
GAGAAAATACGTTTAACGCAGTCTGTGATTTTCTTGCACTTGGAATTGATCCAGATCGATGTACTTTTTGGCTTCAATCT
TCTGTTCCAGAAGTAACGGAACTTGCTTGGTACCTAAGTCATTTTATTTCAGTCAATCAATTGAGTTTGGCACATTCCTA
CAAAGATAAAGTGGCAAAAGGTTTTCATCCTGGAGGCGGACTTTTCTTTTATCCCGTTTTGATGGCTGCGGACATTTTAG
GTTTTGACAGCGATCGAGTTCCAGTTGGTAAAGATCAAAAACAACATTTAGAATTTGCTAGAGATATTGCTTCTACTTTC
AATCGCGAAGTCGGTCCCGTCTTTAAAATTCCCGAACCGGAAATAGATGAAACTACAGCGTTGGTTCCAGGCGTAGATGG
TCAAAAGATGTCTAAGTCTTACGGAAACACGATTAATTTTTTCGACTCCGAAAAGGAACTTAAGAAATCCATAATGTCTA
TCACGACCGACTCCGCCGGGATCGACGAACCCAAAGACCCTTCTAAAAGTAATATTTATGCAATTCATTCTTTGTTTTTA
GATGCCAAAGGAAAAGAAAATCTGAAACAAAAGTTTTTAACCCCAGGAATCGGTTACGGTGATCTAAAGAAACAACTTCT
TCAAGATACTTTGGATTATTTTGCCCCTTATCGAAAAGAACGGGAAAAAATAGGAGCAGACAAAATTTTCGTAGAAGAAT
CTCTAAAAAAAGGACAAACAAAAGCGCGGAAAATAATTTCAAACGTTTTAGACAGAGTTCGCAAAGAATTAGGAATTTAT
CAATTTTAA

Upstream 100 bases:

>100_bases
TAACCTTACATAAAAAAATCGATACAGAACTTGCATCTTTGACGAAAGAATACGAAAGACTTGCTGGTAAACCTTATGAT
ACAAAACGAACGGAGGAAAC

Downstream 100 bases:

>100_bases
ACCATAAATATATTATTATTTTATAAAATATTACTTAACTCCCGACAACAGAGTTGTTGAAAATTTAATTTTTTGCGTCT
TGCATTAAGAGATTGTCCAA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRILTGVQPSGKLHLGNFFSVIRKLKEYQDSTDLFCFIADLHSLTTFSSKEKQRENTFNAVCDFLALGIDPDRCTFWLQS
SVPEVTELAWYLSHFISVNQLSLAHSYKDKVAKGFHPGGGLFFYPVLMAADILGFDSDRVPVGKDQKQHLEFARDIASTF
NREVGPVFKIPEPEIDETTALVPGVDGQKMSKSYGNTINFFDSEKELKKSIMSITTDSAGIDEPKDPSKSNIYAIHSLFL
DAKGKENLKQKFLTPGIGYGDLKKQLLQDTLDYFAPYRKEREKIGADKIFVEESLKKGQTKARKIISNVLDRVRKELGIY
QF

Sequences:

>Translated_322_residues
MRILTGVQPSGKLHLGNFFSVIRKLKEYQDSTDLFCFIADLHSLTTFSSKEKQRENTFNAVCDFLALGIDPDRCTFWLQS
SVPEVTELAWYLSHFISVNQLSLAHSYKDKVAKGFHPGGGLFFYPVLMAADILGFDSDRVPVGKDQKQHLEFARDIASTF
NREVGPVFKIPEPEIDETTALVPGVDGQKMSKSYGNTINFFDSEKELKKSIMSITTDSAGIDEPKDPSKSNIYAIHSLFL
DAKGKENLKQKFLTPGIGYGDLKKQLLQDTLDYFAPYRKEREKIGADKIFVEESLKKGQTKARKIISNVLDRVRKELGIY
QF
>Mature_322_residues
MRILTGVQPSGKLHLGNFFSVIRKLKEYQDSTDLFCFIADLHSLTTFSSKEKQRENTFNAVCDFLALGIDPDRCTFWLQS
SVPEVTELAWYLSHFISVNQLSLAHSYKDKVAKGFHPGGGLFFYPVLMAADILGFDSDRVPVGKDQKQHLEFARDIASTF
NREVGPVFKIPEPEIDETTALVPGVDGQKMSKSYGNTINFFDSEKELKKSIMSITTDSAGIDEPKDPSKSNIYAIHSLFL
DAKGKENLKQKFLTPGIGYGDLKKQLLQDTLDYFAPYRKEREKIGADKIFVEESLKKGQTKARKIISNVLDRVRKELGIY
QF

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=326, Percent_Identity=33.4355828220859, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI41352700, Length=173, Percent_Identity=41.0404624277457, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1789786, Length=330, Percent_Identity=34.8484848484849, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71982800, Length=332, Percent_Identity=31.0240963855422, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71982793, Length=331, Percent_Identity=30.2114803625378, Blast_Score=134, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6320474, Length=345, Percent_Identity=29.8550724637681, Blast_Score=119, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24666151, Length=329, Percent_Identity=30.6990881458967, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36387; Mature: 36387

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILTGVQPSGKLHLGNFFSVIRKLKEYQDSTDLFCFIADLHSLTTFSSKEKQRENTFNA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCDFLALGIDPDRCTFWLQSSVPEVTELAWYLSHFISVNQLSLAHSYKDKVAKGFHPGGG
HHHHHHHCCCHHHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFFYPVLMAADILGFDSDRVPVGKDQKQHLEFARDIASTFNREVGPVFKIPEPEIDETTA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCE
LVPGVDGQKMSKSYGNTINFFDSEKELKKSIMSITTDSAGIDEPKDPSKSNIYAIHSLFL
ECCCCCCHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DAKGKENLKQKFLTPGIGYGDLKKQLLQDTLDYFAPYRKEREKIGADKIFVEESLKKGQT
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KARKIISNVLDRVRKELGIYQF
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRILTGVQPSGKLHLGNFFSVIRKLKEYQDSTDLFCFIADLHSLTTFSSKEKQRENTFNA
CCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCDFLALGIDPDRCTFWLQSSVPEVTELAWYLSHFISVNQLSLAHSYKDKVAKGFHPGGG
HHHHHHHCCCHHHHHEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LFFYPVLMAADILGFDSDRVPVGKDQKQHLEFARDIASTFNREVGPVFKIPEPEIDETTA
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCE
LVPGVDGQKMSKSYGNTINFFDSEKELKKSIMSITTDSAGIDEPKDPSKSNIYAIHSLFL
ECCCCCCHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
DAKGKENLKQKFLTPGIGYGDLKKQLLQDTLDYFAPYRKEREKIGADKIFVEESLKKGQT
CCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
KARKIISNVLDRVRKELGIYQF
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]