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The map label for this gene is tlyB

Identifier: 45656243

GI number: 45656243

Start: 384088

End: 386628

Strand: Reverse

Name: tlyB

Synonym: LIC10339

Alternate gene names: 45656243

Gene position: 386628-384088 (Counterclockwise)

Preceding gene: 45656244

Following gene: 45656242

Centisome position: 9.04

GC content: 40.97

Gene sequence:

>2541_bases
ATGCTGGAATTTACAAAAAGAGCTAAAAGAGTCATCAACGAGATTGCTCAGGATGAGGCGAAGCGTCTTGGTAGCGATTA
TATCGGACCAGAGCACATTCTGCTTGGTCTTCTGAAAGAAGAAGATTCGGTCGCTATCAAAATCCTGAACAACCTCAACA
TTAACCTCAATGAATTGCGTAAAGAGGTAGAAAGAAGAACGCGGGAAGCGTCCGGAGCCTTGCTCATGGACGTAGCCGGT
GGTCAAGATCGTTATCAAAAAATCATTGAACTCTCCAAAGAAGAGGCAAAACGTCTCAAACATAACTATGTGGGAACGGA
ACACATCCTTCTTGCATTGCTTAGAGATAATAATAACATTGCAGGTGGAGCTCTTTATTCTTTTAGCGTAAATTATAATG
TTATAAAAAGCGAAATCTTGCGTTTGCTCGGAGCTCCTCCTACGAGTTCCGTAGGTGTAAGTTCCACTACACAATCAGGT
CCTCAACCTCGTCAGGAAAAAACCAAAACTCCCATCTTGGATGAATTTGCTCGGGATTTAACTCAACTTGCAAGAGACAA
AAAGTTAGATCCAGTTGTAGGAAGGGCTACTGAAATACAAAGAGTGATTCAAATTCTTTCCAGAAAAACCAAGAACAACC
CCGTTCTAGTAGGAGAATCCGGAGTCGGTAAAACCGCAATCGTAGAAGGTTTGGCTCTTGCGATCGTAGAAAAAAGTGTT
CCTGATCTTTTATTTGAGAAAAGAGTTCTTTCTTTGGATCTTGCGTCTTTGATCGCTGGAACTAAATACAGGGGAGAATT
TGAAGAACGTCTGAAAAAGATCATGAAAGAAATTACTTCTTCCACGAATATCATCATCTTTATAGATGAACTTCATACTC
TGATCGGAGCCGGTGCAGCGGAAGGGGCTGTGGATGCTGCTAATATTCTCAAACCTGCTTTAGCAAGAGGAGAATTACAA
TGTATCGGTGCCACTACAAGCGCTGAATATAGAAAATACATCGAAAAAGATTCCGCATTAGAAAGAAGATTCCAAGTCGT
GAAAGTGGCGGAACCTTCCGTAGACGATGCGATCCAAATTTTACAAGGACTCAAAAAAGCTTACGAAACTCATCATAAGG
TGAGATATTCTGATAAGGCTTTGGAACAATCCGTAAAACTTTCTCATAGATATATCAACGATCGTTATCTACCTGACAAA
GCGATTGATATTATCGACGAAGCAGGAGCTAAAGCGCGTTTGGCGAATTGTGCTCGTCCACAAACGATCAAGGATCTGGA
AGAAGAAATCAAATCTCTTGCTTCTAAAAAAGAAGAACTGGTCCGTGCTCAAGAATACGAAAAAGCAGCTGGCGTTCGAG
ACGAAGTGAATCGTAAAAAACAAGCAATGGAAGAAAAACTTCGTTCTTGGCAAGAGAAGATGGAAGACTTTGCGGTTAAT
ATCGAAGAAGACGATATTCTCTCAGTAATTTCTTTATGGACCGGTATTCCTTTGGAAAAAATGGAAGAATCCGAATCCGA
TAAACTACTTCGTTTAGAAGAAGAGTTGAAAAAACGAGTCGTAGGTCAAGCCGAAGCAATCGAAAAAATCGCAAAAGCAG
TTCGTCGCGCTAGAACTGGTTTTAAGAGTGAAAGACGCCCTACCGGTTCTTTTATTTTCTTGGGACCGACCGGTGTGGGT
AAGACAGAACTGGCAAAAGCTCTCGCAAACTTTCTATTCGGCAACGACGACGCAATGCTTCGTGTAGATATGTCTGAATA
CATGGAACCTCACGCAGTAAGCCGTTTAATCGGAGCTCCTCCAGGCTATGTAGGTTATGACGACGGAGGTCAACTGACCG
AATTTGTTAGAAAAAGACCTTATTCCATCATCCTTTTGGATGAGATTGAAAAAGCTCATCACGACATTTTCAACATTCTA
CTCCAGATTATGGAAGAAGGAAACTTAACCGATACGAAAGGACGAAAAGTCAATTTCAGAGATACGATCATCATCATGAC
TTCCAACATAGGCGCAAAAGAAATTCAAGCCGGAGGAAGACTTGGATTTGAAGATCGTAAAGATGAAGCGTTGAAATACA
AGTCTGATCAAACCAGGGATCAATTGAAAAAATATTTCAACCCAGAATTCTTAAACCGTGTCGACGAAGTCATTTACTTC
GGATCTCTCACCAAAGAAAACATCATGTCCATCATAGATATTATGGTGACCGATACGAACAAAAGGCTTCATGAAAAAAC
GATTCAAATTTCCATTACTCAAGCGGCAAAAAATCATATCATGGATATTGGCTACGACGAGAAATTTGGAGCGAGACCTC
TTAGAAGAGTTTTCCAAAGAGAGTTGGAAGATCACATGGCGGTTCAAACTCTCAAAGGCGCTTACAAGGAACCTACAAAA
ATCGAAATCGATTTTAAAGAAGGCAAACTTGAATTCCTAGAAACTCCGTGGACTGACTATAAACCGGTTGATCCTAAAAC
CGGAGGGAACGACAATTCTTCCGGCAATCCTGAAAGGTCGGAAGAAATTGCCTTAGTTTAA

Upstream 100 bases:

>100_bases
CGATACTTAAATTAAAATCAATGATCTGGTTCCCACTTAGGTGGGAATTTCAAAGCGAATTCAGTCTAATCATTAAAAAT
CAAAATACAAAGGCGCTGAT

Downstream 100 bases:

>100_bases
GGATGCCTATACTCATCCCTGTATTGATTCTGATTTCCTATCTTCTGATTCGGAAGATTTGGTTTCATCTTAGGAAAATC
AGAACCATTGCAGGGATCGA

Product: hemolysin B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MLEFTKRAKRVINEIAQDEAKRLGSDYIGPEHILLGLLKEEDSVAIKILNNLNINLNELRKEVERRTREASGALLMDVAG
GQDRYQKIIELSKEEAKRLKHNYVGTEHILLALLRDNNNIAGGALYSFSVNYNVIKSEILRLLGAPPTSSVGVSSTTQSG
PQPRQEKTKTPILDEFARDLTQLARDKKLDPVVGRATEIQRVIQILSRKTKNNPVLVGESGVGKTAIVEGLALAIVEKSV
PDLLFEKRVLSLDLASLIAGTKYRGEFEERLKKIMKEITSSTNIIIFIDELHTLIGAGAAEGAVDAANILKPALARGELQ
CIGATTSAEYRKYIEKDSALERRFQVVKVAEPSVDDAIQILQGLKKAYETHHKVRYSDKALEQSVKLSHRYINDRYLPDK
AIDIIDEAGAKARLANCARPQTIKDLEEEIKSLASKKEELVRAQEYEKAAGVRDEVNRKKQAMEEKLRSWQEKMEDFAVN
IEEDDILSVISLWTGIPLEKMEESESDKLLRLEEELKKRVVGQAEAIEKIAKAVRRARTGFKSERRPTGSFIFLGPTGVG
KTELAKALANFLFGNDDAMLRVDMSEYMEPHAVSRLIGAPPGYVGYDDGGQLTEFVRKRPYSIILLDEIEKAHHDIFNIL
LQIMEEGNLTDTKGRKVNFRDTIIIMTSNIGAKEIQAGGRLGFEDRKDEALKYKSDQTRDQLKKYFNPEFLNRVDEVIYF
GSLTKENIMSIIDIMVTDTNKRLHEKTIQISITQAAKNHIMDIGYDEKFGARPLRRVFQRELEDHMAVQTLKGAYKEPTK
IEIDFKEGKLEFLETPWTDYKPVDPKTGGNDNSSGNPERSEEIALV

Sequences:

>Translated_846_residues
MLEFTKRAKRVINEIAQDEAKRLGSDYIGPEHILLGLLKEEDSVAIKILNNLNINLNELRKEVERRTREASGALLMDVAG
GQDRYQKIIELSKEEAKRLKHNYVGTEHILLALLRDNNNIAGGALYSFSVNYNVIKSEILRLLGAPPTSSVGVSSTTQSG
PQPRQEKTKTPILDEFARDLTQLARDKKLDPVVGRATEIQRVIQILSRKTKNNPVLVGESGVGKTAIVEGLALAIVEKSV
PDLLFEKRVLSLDLASLIAGTKYRGEFEERLKKIMKEITSSTNIIIFIDELHTLIGAGAAEGAVDAANILKPALARGELQ
CIGATTSAEYRKYIEKDSALERRFQVVKVAEPSVDDAIQILQGLKKAYETHHKVRYSDKALEQSVKLSHRYINDRYLPDK
AIDIIDEAGAKARLANCARPQTIKDLEEEIKSLASKKEELVRAQEYEKAAGVRDEVNRKKQAMEEKLRSWQEKMEDFAVN
IEEDDILSVISLWTGIPLEKMEESESDKLLRLEEELKKRVVGQAEAIEKIAKAVRRARTGFKSERRPTGSFIFLGPTGVG
KTELAKALANFLFGNDDAMLRVDMSEYMEPHAVSRLIGAPPGYVGYDDGGQLTEFVRKRPYSIILLDEIEKAHHDIFNIL
LQIMEEGNLTDTKGRKVNFRDTIIIMTSNIGAKEIQAGGRLGFEDRKDEALKYKSDQTRDQLKKYFNPEFLNRVDEVIYF
GSLTKENIMSIIDIMVTDTNKRLHEKTIQISITQAAKNHIMDIGYDEKFGARPLRRVFQRELEDHMAVQTLKGAYKEPTK
IEIDFKEGKLEFLETPWTDYKPVDPKTGGNDNSSGNPERSEEIALV
>Mature_846_residues
MLEFTKRAKRVINEIAQDEAKRLGSDYIGPEHILLGLLKEEDSVAIKILNNLNINLNELRKEVERRTREASGALLMDVAG
GQDRYQKIIELSKEEAKRLKHNYVGTEHILLALLRDNNNIAGGALYSFSVNYNVIKSEILRLLGAPPTSSVGVSSTTQSG
PQPRQEKTKTPILDEFARDLTQLARDKKLDPVVGRATEIQRVIQILSRKTKNNPVLVGESGVGKTAIVEGLALAIVEKSV
PDLLFEKRVLSLDLASLIAGTKYRGEFEERLKKIMKEITSSTNIIIFIDELHTLIGAGAAEGAVDAANILKPALARGELQ
CIGATTSAEYRKYIEKDSALERRFQVVKVAEPSVDDAIQILQGLKKAYETHHKVRYSDKALEQSVKLSHRYINDRYLPDK
AIDIIDEAGAKARLANCARPQTIKDLEEEIKSLASKKEELVRAQEYEKAAGVRDEVNRKKQAMEEKLRSWQEKMEDFAVN
IEEDDILSVISLWTGIPLEKMEESESDKLLRLEEELKKRVVGQAEAIEKIAKAVRRARTGFKSERRPTGSFIFLGPTGVG
KTELAKALANFLFGNDDAMLRVDMSEYMEPHAVSRLIGAPPGYVGYDDGGQLTEFVRKRPYSIILLDEIEKAHHDIFNIL
LQIMEEGNLTDTKGRKVNFRDTIIIMTSNIGAKEIQAGGRLGFEDRKDEALKYKSDQTRDQLKKYFNPEFLNRVDEVIYF
GSLTKENIMSIIDIMVTDTNKRLHEKTIQISITQAAKNHIMDIGYDEKFGARPLRRVFQRELEDHMAVQTLKGAYKEPTK
IEIDFKEGKLEFLETPWTDYKPVDPKTGGNDNSSGNPERSEEIALV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=330, Percent_Identity=31.5151515151515, Blast_Score=189, Evalue=7e-48,
Organism=Escherichia coli, GI1788943, Length=866, Percent_Identity=43.1870669745958, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=803, Percent_Identity=38.9788293897883, Blast_Score=546, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6320464, Length=762, Percent_Identity=44.750656167979, Blast_Score=599, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6323002, Length=898, Percent_Identity=36.0801781737194, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- InterPro:   IPR001943 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 95306; Mature: 95306

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFTKRAKRVINEIAQDEAKRLGSDYIGPEHILLGLLKEEDSVAIKILNNLNINLNELR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
KEVERRTREASGALLMDVAGGQDRYQKIIELSKEEAKRLKHNYVGTEHILLALLRDNNNI
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
AGGALYSFSVNYNVIKSEILRLLGAPPTSSVGVSSTTQSGPQPRQEKTKTPILDEFARDL
CCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHH
TQLARDKKLDPVVGRATEIQRVIQILSRKTKNNPVLVGESGVGKTAIVEGLALAIVEKSV
HHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
PDLLFEKRVLSLDLASLIAGTKYRGEFEERLKKIMKEITSSTNIIIFIDELHTLIGAGAA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCC
EGAVDAANILKPALARGELQCIGATTSAEYRKYIEKDSALERRFQVVKVAEPSVDDAIQI
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHH
LQGLKKAYETHHKVRYSDKALEQSVKLSHRYINDRYLPDKAIDIIDEAGAKARLANCARP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCC
QTIKDLEEEIKSLASKKEELVRAQEYEKAAGVRDEVNRKKQAMEEKLRSWQEKMEDFAVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEEDDILSVISLWTGIPLEKMEESESDKLLRLEEELKKRVVGQAEAIEKIAKAVRRARTG
CCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
FKSERRPTGSFIFLGPTGVGKTELAKALANFLFGNDDAMLRVDMSEYMEPHAVSRLIGAP
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHCCHHHHHHHHCCC
PGYVGYDDGGQLTEFVRKRPYSIILLDEIEKAHHDIFNILLQIMEEGNLTDTKGRKVNFR
CCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
DTIIIMTSNIGAKEIQAGGRLGFEDRKDEALKYKSDQTRDQLKKYFNPEFLNRVDEVIYF
EEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHH
GSLTKENIMSIIDIMVTDTNKRLHEKTIQISITQAAKNHIMDIGYDEKFGARPLRRVFQR
CCCCHHHHHHHHHHHHCCCCHHHHHHHEEEEEHHHHHHCCEECCCCCCCCCHHHHHHHHH
ELEDHMAVQTLKGAYKEPTKIEIDFKEGKLEFLETPWTDYKPVDPKTGGNDNSSGNPERS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
EEIALV
CCCCCC
>Mature Secondary Structure
MLEFTKRAKRVINEIAQDEAKRLGSDYIGPEHILLGLLKEEDSVAIKILNNLNINLNELR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHH
KEVERRTREASGALLMDVAGGQDRYQKIIELSKEEAKRLKHNYVGTEHILLALLRDNNNI
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
AGGALYSFSVNYNVIKSEILRLLGAPPTSSVGVSSTTQSGPQPRQEKTKTPILDEFARDL
CCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHH
TQLARDKKLDPVVGRATEIQRVIQILSRKTKNNPVLVGESGVGKTAIVEGLALAIVEKSV
HHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
PDLLFEKRVLSLDLASLIAGTKYRGEFEERLKKIMKEITSSTNIIIFIDELHTLIGAGAA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCC
EGAVDAANILKPALARGELQCIGATTSAEYRKYIEKDSALERRFQVVKVAEPSVDDAIQI
CCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHH
LQGLKKAYETHHKVRYSDKALEQSVKLSHRYINDRYLPDKAIDIIDEAGAKARLANCARP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCCC
QTIKDLEEEIKSLASKKEELVRAQEYEKAAGVRDEVNRKKQAMEEKLRSWQEKMEDFAVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IEEDDILSVISLWTGIPLEKMEESESDKLLRLEEELKKRVVGQAEAIEKIAKAVRRARTG
CCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
FKSERRPTGSFIFLGPTGVGKTELAKALANFLFGNDDAMLRVDMSEYMEPHAVSRLIGAP
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHCCHHHHHHHHCCC
PGYVGYDDGGQLTEFVRKRPYSIILLDEIEKAHHDIFNILLQIMEEGNLTDTKGRKVNFR
CCCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
DTIIIMTSNIGAKEIQAGGRLGFEDRKDEALKYKSDQTRDQLKKYFNPEFLNRVDEVIYF
EEEEEEECCCCHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHH
GSLTKENIMSIIDIMVTDTNKRLHEKTIQISITQAAKNHIMDIGYDEKFGARPLRRVFQR
CCCCHHHHHHHHHHHHCCCCHHHHHHHEEEEEHHHHHHCCEECCCCCCCCCHHHHHHHHH
ELEDHMAVQTLKGAYKEPTKIEIDFKEGKLEFLETPWTDYKPVDPKTGGNDNSSGNPERS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCC
EEIALV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]