Click here to switch to the map view.

The map label for this gene is fusA

Identifier: 45656176

GI number: 45656176

Start: 308932

End: 311052

Strand: Reverse

Name: fusA

Synonym: LIC10272

Alternate gene names: 45656176

Gene position: 311052-308932 (Counterclockwise)

Preceding gene: 45656179

Following gene: 45656175

Centisome position: 7.27

GC content: 42.34

Gene sequence:

>2121_bases
ATGAGCACTGCCGTAGCCGAATTCAAACCGAGCGAAAAACTTCTAAAAACCAGAAACATTGGAATTTCTGCCCATATCGA
TTCCGGAAAAACAACCCTTACTGAAAGAATTCTATTTTATACAAATCGAATTCACGCAATTCACGAAGTTCGCGGAAAAG
ACGGAGTCGGCGCAAAAATGGATAGTATGGACCTCGAAAGAGAAAGAGGGATTACCATTCAGTCCGCTGCTACCTATTGT
CAGTGGAAAAATCATACGATCAATATTATCGATACACCGGGTCACGTTGACTTTACTGTAGAAGTAGAACGTTCTCTTCG
TGTATTAGATTCCGCAATTTTAGTTCTTTGCGGGGTCGCAGGAGTTCAATCACAGTCGATCACAGTCGATCGTCAGATGA
GACGTTATAATGTTCCTCGCGTCGCATTTATTAATAAACTCGATAGAACTGGAGCCAATCCATTCCGAGTAATTGAACAA
CTTAAAGAAAAACTAAAACACAATGCGGTTCCAGTTCAAATTCCGATCGGTCTTGAAAACGATCTAAAAGGGATTGTAGA
CCTTGTTACGATGAAAGCATACTACTTCGAAGGCAAGGACGGGATGGACATTCAGGAAAAAGAAATTCCGGATGATCTCA
AAGAACTCGCGCAAAAGAAACACGAAGAACTTTTAGACGCGGCTTCTATGTTCTCCGACGAATTGACAGAAGCTCTTCTC
GAAGGAACTCCAACCGAAGAGATGATCAAAAAAGCAATTCGCACCGGCACAATCGAACTCAAGATGACCCCTGTTTTTAT
GGGTTCCGCTTTCAAAAACAAAGGAGTTCAAAAACTTCTGGACGGAGTTTTAGATTATCTGGCAAGCCCTGTAGATGTTA
AAAACAAGGCCTTAGATCAAAACAACAACGAAGAAATGATCGTTCTTGAATCCAATTTTGAGAAGCCTTTGGTTTGTCTC
GCGTTTAAACTCGAAGACGGACGTTACGGTCAGCTTACATACGTGCGTGTTTATCAGGGAAAACTCGCCAAAGGGATGAC
TATCTACAATATGTCGAACAACAAGAAACATAACGTAGGTAGACTTTGTAGAATGCACTCGGATGAAATGGAAGACATCG
ACTCTGCGGAAGCGGGTGATATCATCGCACTTTTCGGGATTGATTGTGCTTCCGGGGATACTTTTACCGATGGAAAACTT
AAGGTTTCCATGGAATCTATGTTCGTTCCTGCTCCAGTGATCTCTTTAACAATCGAAGCGAAGGAATCGAAACACCTAAA
CAATCTTGCAAAAGCGTTAAACCGTTTTACCAAGGAAGATCCAACCTTTCAAACACATGTAGATCAAGAGTCCGGTCAGA
CCATCATCAAAGGAATGGGAGAACTCCATCTCGAAGTTTATATCGAACGTATGAAAAGAGAGTACGGTGTGGAATTGATC
ACAGGCGCTCCTCAAGTAGCTTACCGTGAAACGATCACTTCCAAAGCAGATTTCGATTATACTCATAAAAAACAAACCGG
TGGTCAAGGTCAGTTCGGTCGTGTCGCAGGTTATATGGAACCGATCCCGCTTGAAGAAACATTAGATTACGATTTCGTAA
ACAAGGTAGTGGGTGGTGCGATCCCAAGAGAATACATTCAATCGGTAGACAAAGGATTTAAAAGTTGTTTAGAGCGAGGT
TCTTTGATCGGGTTTCCTATCATTGGAGTTCGCTGTGTGATCAACGATGGTGCTTATCACGATGTGGACTCTTCCGATAT
GGCGTTCCAAATTGCCGGTCGTTATGCGTTCCGCCAAGGATTCAACAAGGCAAATCCTCAAATTCTAGAGCCGATTATGA
AAGTCGAAGTTGATGGTCCTTCTGAATTCCAAGGAGCAATCCTCGGATCTTTGAACCAAAGACGCGGTATGATTCTGAAC
ACTACTGAAGAAGACGCTTATTGTAAAACCGAGGCGGAAGTTCCTCTTGCAGATATGTTCGGATATTCAACTGTATTACG
TTCGTCTACACAGGGTAAGGCGGAGTTTTCGATGGAATTTTCCAGATATGCTCCAGTTCCAAGAAACGTAGCGGAAGAAT
TGATGAAAAAATACAAGGTCAACAATAAAGACGAAGATTGA

Upstream 100 bases:

>100_bases
TCGCCCTTTTTTGGAATTTGGTAAATTGAGGGACTTTCTCCAAAGCTCCCTACCGGTGTTTTTACCGGAGACATTTTGCA
ATCTTTTATAAGAGGATGCC

Downstream 100 bases:

>100_bases
TCTAATTATTTCAAAGACTTTTTGAAGTGAACTTTTTATAAAAGTTCTTACCCTTATTTTTGTGAAAAAATAAAATTTTT
TGAGGCGAATTTCTAAAATG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MSTAVAEFKPSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHAIHEVRGKDGVGAKMDSMDLERERGITIQSAATYC
QWKNHTINIIDTPGHVDFTVEVERSLRVLDSAILVLCGVAGVQSQSITVDRQMRRYNVPRVAFINKLDRTGANPFRVIEQ
LKEKLKHNAVPVQIPIGLENDLKGIVDLVTMKAYYFEGKDGMDIQEKEIPDDLKELAQKKHEELLDAASMFSDELTEALL
EGTPTEEMIKKAIRTGTIELKMTPVFMGSAFKNKGVQKLLDGVLDYLASPVDVKNKALDQNNNEEMIVLESNFEKPLVCL
AFKLEDGRYGQLTYVRVYQGKLAKGMTIYNMSNNKKHNVGRLCRMHSDEMEDIDSAEAGDIIALFGIDCASGDTFTDGKL
KVSMESMFVPAPVISLTIEAKESKHLNNLAKALNRFTKEDPTFQTHVDQESGQTIIKGMGELHLEVYIERMKREYGVELI
TGAPQVAYRETITSKADFDYTHKKQTGGQGQFGRVAGYMEPIPLEETLDYDFVNKVVGGAIPREYIQSVDKGFKSCLERG
SLIGFPIIGVRCVINDGAYHDVDSSDMAFQIAGRYAFRQGFNKANPQILEPIMKVEVDGPSEFQGAILGSLNQRRGMILN
TTEEDAYCKTEAEVPLADMFGYSTVLRSSTQGKAEFSMEFSRYAPVPRNVAEELMKKYKVNNKDED

Sequences:

>Translated_706_residues
MSTAVAEFKPSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHAIHEVRGKDGVGAKMDSMDLERERGITIQSAATYC
QWKNHTINIIDTPGHVDFTVEVERSLRVLDSAILVLCGVAGVQSQSITVDRQMRRYNVPRVAFINKLDRTGANPFRVIEQ
LKEKLKHNAVPVQIPIGLENDLKGIVDLVTMKAYYFEGKDGMDIQEKEIPDDLKELAQKKHEELLDAASMFSDELTEALL
EGTPTEEMIKKAIRTGTIELKMTPVFMGSAFKNKGVQKLLDGVLDYLASPVDVKNKALDQNNNEEMIVLESNFEKPLVCL
AFKLEDGRYGQLTYVRVYQGKLAKGMTIYNMSNNKKHNVGRLCRMHSDEMEDIDSAEAGDIIALFGIDCASGDTFTDGKL
KVSMESMFVPAPVISLTIEAKESKHLNNLAKALNRFTKEDPTFQTHVDQESGQTIIKGMGELHLEVYIERMKREYGVELI
TGAPQVAYRETITSKADFDYTHKKQTGGQGQFGRVAGYMEPIPLEETLDYDFVNKVVGGAIPREYIQSVDKGFKSCLERG
SLIGFPIIGVRCVINDGAYHDVDSSDMAFQIAGRYAFRQGFNKANPQILEPIMKVEVDGPSEFQGAILGSLNQRRGMILN
TTEEDAYCKTEAEVPLADMFGYSTVLRSSTQGKAEFSMEFSRYAPVPRNVAEELMKKYKVNNKDED
>Mature_705_residues
STAVAEFKPSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHAIHEVRGKDGVGAKMDSMDLERERGITIQSAATYCQ
WKNHTINIIDTPGHVDFTVEVERSLRVLDSAILVLCGVAGVQSQSITVDRQMRRYNVPRVAFINKLDRTGANPFRVIEQL
KEKLKHNAVPVQIPIGLENDLKGIVDLVTMKAYYFEGKDGMDIQEKEIPDDLKELAQKKHEELLDAASMFSDELTEALLE
GTPTEEMIKKAIRTGTIELKMTPVFMGSAFKNKGVQKLLDGVLDYLASPVDVKNKALDQNNNEEMIVLESNFEKPLVCLA
FKLEDGRYGQLTYVRVYQGKLAKGMTIYNMSNNKKHNVGRLCRMHSDEMEDIDSAEAGDIIALFGIDCASGDTFTDGKLK
VSMESMFVPAPVISLTIEAKESKHLNNLAKALNRFTKEDPTFQTHVDQESGQTIIKGMGELHLEVYIERMKREYGVELIT
GAPQVAYRETITSKADFDYTHKKQTGGQGQFGRVAGYMEPIPLEETLDYDFVNKVVGGAIPREYIQSVDKGFKSCLERGS
LIGFPIIGVRCVINDGAYHDVDSSDMAFQIAGRYAFRQGFNKANPQILEPIMKVEVDGPSEFQGAILGSLNQRRGMILNT
TEEDAYCKTEAEVPLADMFGYSTVLRSSTQGKAEFSMEFSRYAPVPRNVAEELMKKYKVNNKDED

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=697, Percent_Identity=53.6585365853659, Blast_Score=755, Evalue=0.0,
Organism=Homo sapiens, GI19923640, Length=710, Percent_Identity=35.4929577464789, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=40.253164556962, Blast_Score=256, Evalue=5e-68,
Organism=Homo sapiens, GI25306287, Length=288, Percent_Identity=44.7916666666667, Blast_Score=221, Evalue=2e-57,
Organism=Homo sapiens, GI4503483, Length=482, Percent_Identity=24.4813278008299, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=37.3333333333333, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=41.1764705882353, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI310132016, Length=112, Percent_Identity=39.2857142857143, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=112, Percent_Identity=39.2857142857143, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=112, Percent_Identity=39.2857142857143, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789738, Length=698, Percent_Identity=48.2808022922636, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI1790835, Length=482, Percent_Identity=27.8008298755187, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=38.6363636363636, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=38.4105960264901, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=705, Percent_Identity=50.0709219858156, Blast_Score=705, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17556745, Length=726, Percent_Identity=29.2011019283747, Blast_Score=281, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17506493, Length=820, Percent_Identity=25.3658536585366, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17557151, Length=192, Percent_Identity=36.9791666666667, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=201, Percent_Identity=30.3482587064677, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=201, Percent_Identity=30.3482587064677, Blast_Score=79, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=689, Percent_Identity=56.8940493468795, Blast_Score=800, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322359, Length=775, Percent_Identity=29.2903225806452, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6324707, Length=424, Percent_Identity=26.6509433962264, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320593, Length=424, Percent_Identity=26.6509433962264, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=199, Percent_Identity=31.1557788944724, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=34.2465753424658, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582462, Length=700, Percent_Identity=54.5714285714286, Blast_Score=784, Evalue=0.0,
Organism=Drosophila melanogaster, GI221458488, Length=726, Percent_Identity=30.9917355371901, Blast_Score=318, Evalue=7e-87,
Organism=Drosophila melanogaster, GI24585711, Length=497, Percent_Identity=25.9557344064386, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585713, Length=497, Percent_Identity=25.9557344064386, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24585709, Length=497, Percent_Identity=25.9557344064386, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI78706572, Length=151, Percent_Identity=39.0728476821192, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574573, Length=143, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 79002; Mature: 78871

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVAEFKPSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHAIHEVRGKDGVGAKM
CCCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSMDLERERGITIQSAATYCQWKNHTINIIDTPGHVDFTVEVERSLRVLDSAILVLCGVA
CCCCCHHHCCCEEECHHHHEEECCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCC
GVQSQSITVDRQMRRYNVPRVAFINKLDRTGANPFRVIEQLKEKLKHNAVPVQIPIGLEN
CCCCCCEEHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCH
DLKGIVDLVTMKAYYFEGKDGMDIQEKEIPDDLKELAQKKHEELLDAASMFSDELTEALL
HHHHHHHHHHHHHEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTPTEEMIKKAIRTGTIELKMTPVFMGSAFKNKGVQKLLDGVLDYLASPVDVKNKALDQ
CCCCHHHHHHHHHHCCEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCC
NNNEEMIVLESNFEKPLVCLAFKLEDGRYGQLTYVRVYQGKLAKGMTIYNMSNNKKHNVG
CCCCCEEEECCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCHH
RLCRMHSDEMEDIDSAEAGDIIALFGIDCASGDTFTDGKLKVSMESMFVPAPVISLTIEA
HHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHHCCCCCEEEEEEEC
KESKHLNNLAKALNRFTKEDPTFQTHVDQESGQTIIKGMGELHLEVYIERMKREYGVELI
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TGAPQVAYRETITSKADFDYTHKKQTGGQGQFGRVAGYMEPIPLEETLDYDFVNKVVGGA
ECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCHHHHHHHHCCC
IPREYIQSVDKGFKSCLERGSLIGFPIIGVRCVINDGAYHDVDSSDMAFQIAGRYAFRQG
CCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHC
FNKANPQILEPIMKVEVDGPSEFQGAILGSLNQRRGMILNTTEEDAYCKTEAEVPLADMF
CCCCCHHHHHHHHHEECCCCCHHHHHHHHCCHHCCCEEEECCCCCCEECCCCCCCHHHHH
GYSTVLRSSTQGKAEFSMEFSRYAPVPRNVAEELMKKYKVNNKDED
HHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STAVAEFKPSEKLLKTRNIGISAHIDSGKTTLTERILFYTNRIHAIHEVRGKDGVGAKM
CCCCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DSMDLERERGITIQSAATYCQWKNHTINIIDTPGHVDFTVEVERSLRVLDSAILVLCGVA
CCCCCHHHCCCEEECHHHHEEECCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHCCC
GVQSQSITVDRQMRRYNVPRVAFINKLDRTGANPFRVIEQLKEKLKHNAVPVQIPIGLEN
CCCCCCEEHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCH
DLKGIVDLVTMKAYYFEGKDGMDIQEKEIPDDLKELAQKKHEELLDAASMFSDELTEALL
HHHHHHHHHHHHHEEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGTPTEEMIKKAIRTGTIELKMTPVFMGSAFKNKGVQKLLDGVLDYLASPVDVKNKALDQ
CCCCHHHHHHHHHHCCEEEEEEEEEEECCHHHCCCHHHHHHHHHHHHCCCCCHHHHHCCC
NNNEEMIVLESNFEKPLVCLAFKLEDGRYGQLTYVRVYQGKLAKGMTIYNMSNNKKHNVG
CCCCCEEEECCCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCHH
RLCRMHSDEMEDIDSAEAGDIIALFGIDCASGDTFTDGKLKVSMESMFVPAPVISLTIEA
HHHHHCCCHHHHCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHHCCCCCEEEEEEEC
KESKHLNNLAKALNRFTKEDPTFQTHVDQESGQTIIKGMGELHLEVYIERMKREYGVELI
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
TGAPQVAYRETITSKADFDYTHKKQTGGQGQFGRVAGYMEPIPLEETLDYDFVNKVVGGA
ECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHCCHHHHHHHHCCC
IPREYIQSVDKGFKSCLERGSLIGFPIIGVRCVINDGAYHDVDSSDMAFQIAGRYAFRQG
CCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEEECCCCCCCCCCCCEEEEHHHHHHHHC
FNKANPQILEPIMKVEVDGPSEFQGAILGSLNQRRGMILNTTEEDAYCKTEAEVPLADMF
CCCCCHHHHHHHHHEECCCCCHHHHHHHHCCHHCCCEEEECCCCCCEECCCCCCCHHHHH
GYSTVLRSSTQGKAEFSMEFSRYAPVPRNVAEELMKKYKVNNKDED
HHHHHHHCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA