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The map label for this gene is ybjF [C]

Identifier: 45656154

GI number: 45656154

Start: 283507

End: 284688

Strand: Direct

Name: ybjF [C]

Synonym: LIC10249

Alternate gene names: 45656154

Gene position: 283507-284688 (Clockwise)

Preceding gene: 45656153

Following gene: 45656155

Centisome position: 6.63

GC content: 34.86

Gene sequence:

>1182_bases
ATGAAACCTCCTGTGAATCAATCTTGTCAACATTATCCGGAATGTGCCGGTTGTGATCGATTGCATATCGGTTACGAAAA
ACAACTTCAACATAAACAAGAAGAGATCGAAAAACGGTTCAAGGGTTTTAAAGGTCTGGAAATTCGGCAGATCATAAAAA
GTCCAAAGGATCAAATGTATCGTCATAAAGTTCAACTTCCGTTTGGACATCGTAAGATAGGAAAAAAATCCGTACTTACT
CTTGGTCTTCATAATAAAGAAAATACGTTTATCATCGATCAGAAAGAATGTAGAATTCAGGATGAAGATTTGACTACCGT
GGCCGCTGCGATCCGCCATTGGGCGCGGAACGAAAATTTAGAACCGTATCATGAAAAAAAAGGAAGTGGACTTTTAAGAC
ATATCGTTCTTAGAAAAGCGAATGCGACTCAAGAAATTCTTGTGGGAATTGTCACTAACGAAAGTGAGATTCCCGGAAGA
AAAAAACTCACAGATAGATTATATTCGTATATTCAACAATTTTTATATAAAGAAAATTCAAAAGCAGATGTAGTAGGAAT
ATTACAAAATGTAAACCGTAAAAACACTAAAGTAGTTTTAGGAGAAAAGGAAGTCACTTGGTACGGAAGACATTTTGTAA
AAGAAAAAATCGGTAAACTTGATTTTCAGATTGGGCTTTCTACATTCTTTCAAGTAAATCCGTTTCAAATAGAAAATTTA
TATAATCTTATTTTGGAAGACCTTCCGGAGAATAAGTGTGTAGTGGACGCTTATTGTGGAATCGGTACAATTTCATTGTA
TATCGCGTCTAAATCTAAAAAAGTAATTGGACTTGAAGAAAATTCCAGTTCGATTCGTTCTGCAATTGGAGCTTCAAAGG
CGAATGGAATCGAAAACGTTCATTTTATTAAGGGAAAGGTTTTAGATACTCTACGAGCAGCCTTAAACGAAAATTCAGAT
GTTGTAGTATTGGATCCGCCGAGAGAAGGACTTGACGCAGAGACTAAAAGCATATTATTAAATTCTAAAGTAAATCAGAT
TCTTTACGTTTCTTGTAATCCGGAAACTCTTCTAAGGGATGCAATTGAGCTTACAAAAAGTTTTAAATACGAAAAGATTA
CTCCTGTGGATTTATTTCCTCATACAAGTCATTTGGAAAGTGTTTCCGTTTTTACAAAATGA

Upstream 100 bases:

>100_bases
TACGATCCAAGATAAGTACAGAAAAACTTATTTGATGTGATCGCTTTTAAAAAACGAATTCAATTGACAGGTACTAAAAG
AACAAAAGCCTGAGCGAAGA

Downstream 100 bases:

>100_bases
TTTTGTAAAAACGTATTTTAAGAGTTTTAGCTTGTTTTATTTTTGTATGAGTTTTTACATTCTAAAGTTTTTGAAAGTTT
TACTCATCCCTATCAAATTG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKPPVNQSCQHYPECAGCDRLHIGYEKQLQHKQEEIEKRFKGFKGLEIRQIIKSPKDQMYRHKVQLPFGHRKIGKKSVLT
LGLHNKENTFIIDQKECRIQDEDLTTVAAAIRHWARNENLEPYHEKKGSGLLRHIVLRKANATQEILVGIVTNESEIPGR
KKLTDRLYSYIQQFLYKENSKADVVGILQNVNRKNTKVVLGEKEVTWYGRHFVKEKIGKLDFQIGLSTFFQVNPFQIENL
YNLILEDLPENKCVVDAYCGIGTISLYIASKSKKVIGLEENSSSIRSAIGASKANGIENVHFIKGKVLDTLRAALNENSD
VVVLDPPREGLDAETKSILLNSKVNQILYVSCNPETLLRDAIELTKSFKYEKITPVDLFPHTSHLESVSVFTK

Sequences:

>Translated_393_residues
MKPPVNQSCQHYPECAGCDRLHIGYEKQLQHKQEEIEKRFKGFKGLEIRQIIKSPKDQMYRHKVQLPFGHRKIGKKSVLT
LGLHNKENTFIIDQKECRIQDEDLTTVAAAIRHWARNENLEPYHEKKGSGLLRHIVLRKANATQEILVGIVTNESEIPGR
KKLTDRLYSYIQQFLYKENSKADVVGILQNVNRKNTKVVLGEKEVTWYGRHFVKEKIGKLDFQIGLSTFFQVNPFQIENL
YNLILEDLPENKCVVDAYCGIGTISLYIASKSKKVIGLEENSSSIRSAIGASKANGIENVHFIKGKVLDTLRAALNENSD
VVVLDPPREGLDAETKSILLNSKVNQILYVSCNPETLLRDAIELTKSFKYEKITPVDLFPHTSHLESVSVFTK
>Mature_393_residues
MKPPVNQSCQHYPECAGCDRLHIGYEKQLQHKQEEIEKRFKGFKGLEIRQIIKSPKDQMYRHKVQLPFGHRKIGKKSVLT
LGLHNKENTFIIDQKECRIQDEDLTTVAAAIRHWARNENLEPYHEKKGSGLLRHIVLRKANATQEILVGIVTNESEIPGR
KKLTDRLYSYIQQFLYKENSKADVVGILQNVNRKNTKVVLGEKEVTWYGRHFVKEKIGKLDFQIGLSTFFQVNPFQIENL
YNLILEDLPENKCVVDAYCGIGTISLYIASKSKKVIGLEENSSSIRSAIGASKANGIENVHFIKGKVLDTLRAALNENSD
VVVLDPPREGLDAETKSILLNSKVNQILYVSCNPETLLRDAIELTKSFKYEKITPVDLFPHTSHLESVSVFTK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family

Homologues:

Organism=Homo sapiens, GI34222389, Length=377, Percent_Identity=25.7294429708223, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI51173878, Length=377, Percent_Identity=25.7294429708223, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI269784680, Length=376, Percent_Identity=24.7340425531915, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI269784676, Length=376, Percent_Identity=24.7340425531915, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI20149688, Length=376, Percent_Identity=24.7340425531915, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI269784678, Length=205, Percent_Identity=29.2682926829268, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1787083, Length=385, Percent_Identity=23.1168831168831, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789148, Length=399, Percent_Identity=24.812030075188, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790403, Length=154, Percent_Identity=31.1688311688312, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322909, Length=364, Percent_Identity=26.0989010989011, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666579, Length=418, Percent_Identity=25.5980861244019, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y249_LEPIC (Q72VP7)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000240.1
- ProteinModelPortal:   Q72VP7
- SMR:   Q72VP7
- GeneID:   2770307
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10249
- HOGENOM:   HBG690163
- OMA:   CGGCKLM
- ProtClustDB:   CLSK573435
- BioCyc:   LINT267671:LIC_10249-MONOMER
- InterPro:   IPR010280

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: 2.1.1.- [C]

Molecular weight: Translated: 44782; Mature: 44782

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 352-352 BINDING 231-231 BINDING 258-258 BINDING 279-279 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPVNQSCQHYPECAGCDRLHIGYEKQLQHKQEEIEKRFKGFKGLEIRQIIKSPKDQMY
CCCCCCCCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHH
RHKVQLPFGHRKIGKKSVLTLGLHNKENTFIIDQKECRIQDEDLTTVAAAIRHWARNENL
HHEECCCCCCHHCCCCCEEEEEEECCCCEEEEECHHCCCCCCHHHHHHHHHHHHHCCCCC
EPYHEKKGSGLLRHIVLRKANATQEILVGIVTNESEIPGRKKLTDRLYSYIQQFLYKENS
CHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
KADVVGILQNVNRKNTKVVLGEKEVTWYGRHFVKEKIGKLDFQIGLSTFFQVNPFQIENL
CCHHHHHHHHCCCCCCEEEECCCCEEHHHHHHHHHHHCCCEEEECHHHHEECCCCHHHHH
YNLILEDLPENKCVVDAYCGIGTISLYIASKSKKVIGLEENSSSIRSAIGASKANGIENV
HHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCEEEEECCCHHHHHHHHCCHHCCCCCCE
HFIKGKVLDTLRAALNENSDVVVLDPPREGLDAETKSILLNSKVNQILYVSCNPETLLRD
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHH
AIELTKSFKYEKITPVDLFPHTSHLESVSVFTK
HHHHHHCCCCCCCCCEECCCCCCCCCCHHHCCC
>Mature Secondary Structure
MKPPVNQSCQHYPECAGCDRLHIGYEKQLQHKQEEIEKRFKGFKGLEIRQIIKSPKDQMY
CCCCCCCCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHH
RHKVQLPFGHRKIGKKSVLTLGLHNKENTFIIDQKECRIQDEDLTTVAAAIRHWARNENL
HHEECCCCCCHHCCCCCEEEEEEECCCCEEEEECHHCCCCCCHHHHHHHHHHHHHCCCCC
EPYHEKKGSGLLRHIVLRKANATQEILVGIVTNESEIPGRKKLTDRLYSYIQQFLYKENS
CHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
KADVVGILQNVNRKNTKVVLGEKEVTWYGRHFVKEKIGKLDFQIGLSTFFQVNPFQIENL
CCHHHHHHHHCCCCCCEEEECCCCEEHHHHHHHHHHHCCCEEEECHHHHEECCCCHHHHH
YNLILEDLPENKCVVDAYCGIGTISLYIASKSKKVIGLEENSSSIRSAIGASKANGIENV
HHHHHHHCCCCCEEEEECCCCCEEEEEEEECCCEEEEECCCHHHHHHHHCCHHCCCCCCE
HFIKGKVLDTLRAALNENSDVVVLDPPREGLDAETKSILLNSKVNQILYVSCNPETLLRD
EEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHH
AIELTKSFKYEKITPVDLFPHTSHLESVSVFTK
HHHHHHCCCCCCCCCEECCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA