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The map label for this gene is mesJ

Identifier: 45656041

GI number: 45656041

Start: 155046

End: 156347

Strand: Reverse

Name: mesJ

Synonym: LIC10131

Alternate gene names: 45656041

Gene position: 156347-155046 (Counterclockwise)

Preceding gene: 45656043

Following gene: 45656040

Centisome position: 3.66

GC content: 35.94

Gene sequence:

>1302_bases
ATGAGAGATAAAATCTCCGAATCCACACAAAAGATTTTCCATGCAGTATGGGAAAGAATTGCTCCCTTTCACGAGATGAT
CCAGTCTCGTCCCGCGGTTCTTTCTTACTCCGGCGGAAAAGATTCATCTATTTTGTTGCATTTTTATTTCTGGCTGTGGA
TAGAAAAAAAAATACCTGCGCCTTGTATTTATCATCTAGACCATTCGATCCGATTCAATTTAGAACAGGAAAAAAAAATC
TTTGAATATGCAGACAGTACATTTCCATTTTCTAAAATATTCAAAAAAAAGAATATTCCTGCTTTGTCAAGAAGGTTAGG
CAAAACGCTTGAAGAGACAGGAAGGGCGTTTCGATATAAGGATTTAAAAAAAATTTCGGATCAATACGAAGGTTATATAG
TCACTGGTCATCACTCGAGTGATTATCTGGAAACGATTCTTCTTAACTTAATTCGAGGAGGGGGTTGGAATTCTTTACGA
ACCTTAGGATGGTATGAAAAAAACCGCTTTAGACCTCTATTTGCATTCTCCCAAGAGGAAATTGAAACAATTTTACAATC
CGAATTTTGGAAAATTTTTGAAGATGAATCTAACAATAGCGATGAATATCTTAGAAATCGAATTCGAAGCTATATTATAC
CTTTATTATTAAGAGAAGGGGCCAACCCAGATCGAATCTATAAAAACTTCCATAGGATAGAAAAACCAGTCTCTAAAATT
TTTTCCAAAAAAAACTCAGATCATAAAATTCCTTCTTTTTTAAAAATAGATATTTGGGTTTTGAACGATCTTTCTCAAAG
AGAAAGAAAATTCTTTATAGATCGTTATCTCCGTTCCTTAAACTTATACCCTACAACACGAAATTTTTTCAGAGATCTTA
CGGATCTTTTACAAAAAGAAAACTCGTTCAGCCTTGAAAACAAAGAGACATGGTTTTGGAAATCGACTTCTTCCGACCTT
TATCTGATTCCTAAAAACTCTCCTTGTTTAAGAGAATTTAGATTTGAACCGAAAGAGATGGTTCTCAAATGGAACGGAAA
TCAAAAAAAAATCCCTCCAGATTTAATTCCGGATTTATGCCCTGCAGGAGCCAAAATCCGCAAAAACGGGATGAGTATAG
AAATTTCCGAAATCCTACGACAAAAAGAGATTCCAGTTCCGGTTAGAAAAATGCTACCCATACTTCGCGGGGAAAGGAAA
GTTGATGTGATCTGTCTGAGTCTTTGGGATCCTAAAATAGGCGACATCGTAGCGGACAGAGAGGTTGAAATTTTACCTGA
CTTTCAGGAGCCCGGAGTATGA

Upstream 100 bases:

>100_bases
TTGAGTTTCTATAAAATTGTTCTAAACGGAATAATCCAGAAATCAATGAATAGAAACGATACGACTCTTGGGCAATCCGA
AGAACCGTTAAGTCAGTACG

Downstream 100 bases:

>100_bases
ACGAGGATCCTCAAAAAAAGGACGAACCATTCTTTAAAGACGTTGAGTTCAAAGCATCTTACGGAAAAGCAGATCAAATC
CCTTCTCAAGGAACACCTCA

Product: cell cycle protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRDKISESTQKIFHAVWERIAPFHEMIQSRPAVLSYSGGKDSSILLHFYFWLWIEKKIPAPCIYHLDHSIRFNLEQEKKI
FEYADSTFPFSKIFKKKNIPALSRRLGKTLEETGRAFRYKDLKKISDQYEGYIVTGHHSSDYLETILLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFSQEEIETILQSEFWKIFEDESNNSDEYLRNRIRSYIIPLLLREGANPDRIYKNFHRIEKPVSKI
FSKKNSDHKIPSFLKIDIWVLNDLSQRERKFFIDRYLRSLNLYPTTRNFFRDLTDLLQKENSFSLENKETWFWKSTSSDL
YLIPKNSPCLREFRFEPKEMVLKWNGNQKKIPPDLIPDLCPAGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILRGERK
VDVICLSLWDPKIGDIVADREVEILPDFQEPGV

Sequences:

>Translated_433_residues
MRDKISESTQKIFHAVWERIAPFHEMIQSRPAVLSYSGGKDSSILLHFYFWLWIEKKIPAPCIYHLDHSIRFNLEQEKKI
FEYADSTFPFSKIFKKKNIPALSRRLGKTLEETGRAFRYKDLKKISDQYEGYIVTGHHSSDYLETILLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFSQEEIETILQSEFWKIFEDESNNSDEYLRNRIRSYIIPLLLREGANPDRIYKNFHRIEKPVSKI
FSKKNSDHKIPSFLKIDIWVLNDLSQRERKFFIDRYLRSLNLYPTTRNFFRDLTDLLQKENSFSLENKETWFWKSTSSDL
YLIPKNSPCLREFRFEPKEMVLKWNGNQKKIPPDLIPDLCPAGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILRGERK
VDVICLSLWDPKIGDIVADREVEILPDFQEPGV
>Mature_433_residues
MRDKISESTQKIFHAVWERIAPFHEMIQSRPAVLSYSGGKDSSILLHFYFWLWIEKKIPAPCIYHLDHSIRFNLEQEKKI
FEYADSTFPFSKIFKKKNIPALSRRLGKTLEETGRAFRYKDLKKISDQYEGYIVTGHHSSDYLETILLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFSQEEIETILQSEFWKIFEDESNNSDEYLRNRIRSYIIPLLLREGANPDRIYKNFHRIEKPVSKI
FSKKNSDHKIPSFLKIDIWVLNDLSQRERKFFIDRYLRSLNLYPTTRNFFRDLTDLLQKENSFSLENKETWFWKSTSSDL
YLIPKNSPCLREFRFEPKEMVLKWNGNQKKIPPDLIPDLCPAGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILRGERK
VDVICLSLWDPKIGDIVADREVEILPDFQEPGV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=204, Percent_Identity=28.4313725490196, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_LEPIC (Q72W10)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000127.1
- ProteinModelPortal:   Q72W10
- GeneID:   2771663
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10131
- NMPDR:   fig|267671.1.peg.127
- HOGENOM:   HBG110208
- OMA:   RIAPFHE
- ProtClustDB:   CLSK573376
- BioCyc:   LINT267671:LIC_10131-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 51394; Mature: 51394

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKISESTQKIFHAVWERIAPFHEMIQSRPAVLSYSGGKDSSILLHFYFWLWIEKKIPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
PCIYHLDHSIRFNLEQEKKIFEYADSTFPFSKIFKKKNIPALSRRLGKTLEETGRAFRYK
CEEEECCCCEEECCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHH
DLKKISDQYEGYIVTGHHSSDYLETILLNLIRGGGWNSLRTLGWYEKNRFRPLFAFSQEE
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCCHHHHCCHHH
IETILQSEFWKIFEDESNNSDEYLRNRIRSYIIPLLLREGANPDRIYKNFHRIEKPVSKI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FSKKNSDHKIPSFLKIDIWVLNDLSQRERKFFIDRYLRSLNLYPTTRNFFRDLTDLLQKE
HHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
NSFSLENKETWFWKSTSSDLYLIPKNSPCLREFRFEPKEMVLKWNGNQKKIPPDLIPDLC
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHC
PAGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILRGERKVDVICLSLWDPKIGDIVADR
CCCCHHHHCCCEEEHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCC
EVEILPDFQEPGV
CEEECCCCCCCCC
>Mature Secondary Structure
MRDKISESTQKIFHAVWERIAPFHEMIQSRPAVLSYSGGKDSSILLHFYFWLWIEKKIPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCC
PCIYHLDHSIRFNLEQEKKIFEYADSTFPFSKIFKKKNIPALSRRLGKTLEETGRAFRYK
CEEEECCCCEEECCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHH
DLKKISDQYEGYIVTGHHSSDYLETILLNLIRGGGWNSLRTLGWYEKNRFRPLFAFSQEE
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCCHHHHCCHHH
IETILQSEFWKIFEDESNNSDEYLRNRIRSYIIPLLLREGANPDRIYKNFHRIEKPVSKI
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
FSKKNSDHKIPSFLKIDIWVLNDLSQRERKFFIDRYLRSLNLYPTTRNFFRDLTDLLQKE
HHCCCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
NSFSLENKETWFWKSTSSDLYLIPKNSPCLREFRFEPKEMVLKWNGNQKKIPPDLIPDLC
CCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCHHHEEEEECCCCCCCCHHHHHHHC
PAGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILRGERKVDVICLSLWDPKIGDIVADR
CCCCHHHHCCCEEEHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCC
EVEILPDFQEPGV
CEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA