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The map label for this gene is hisS

Identifier: 45655962

GI number: 45655962

Start: 57920

End: 59239

Strand: Reverse

Name: hisS

Synonym: LIC10048

Alternate gene names: 45655962

Gene position: 59239-57920 (Counterclockwise)

Preceding gene: 45655963

Following gene: 45655961

Centisome position: 1.38

GC content: 36.29

Gene sequence:

>1320_bases
TTGGAAAATCAGAAAAACTTTTTAACCACAGCTCCTTACAAGGGGACCAGAGATTTTTATCCGGAGGATATGAGACTTCG
GAACTGGATGTTTTCCGTTATGCGGGAAACCGTTCTCTCTTTCGGCTACGAGGAATACGACGGTCCTATTTTAGAATCCT
TCGATCTCTACAAAGCTAAAAGCGGAGAAGAAATCGTTGAACGGCAGTTATACGATTTTATAGATAAAGGAGAAAGAAGA
GTTGCAATCCGTCCTGAGATGACTCCTACGCTCGCAAGGATGGTAGCAGGAAATTTACGCAATCTTCCTAAACCTGTACG
TTGGTTTTCTATTCCTAATTTATGGCGCTACGAACAACCTGGCAAGGGTAGACTCAGAGAGCACTGGCAATTGAATGTGG
ATCTTTTTGGAGTGGATTCTCATCGCGCCGAACTTGAAATTCTTTTGATTGCGGATTCGATTTTAAAAAAGTTCGGAGCA
CCGATTGGAAGTTATCAAATCAAAGTTTCTCATAGAAAACTTTTAGATAGTTTTTTAAAAAATTCCCTCAAACTCAATGG
TGATCAAGTTCACGGGGTTTCTAAACTTTTAGATAAAAAATCTAAAATTTCTTCTGAAGCTTTCGAAACAGAAATGAAAC
CTTTTTTAAATAATTTTAAAGAACAATTCTCCCTGATTGAAACGTATCTCAATTCTAATTTAGAAACAGTTTCTAAGATT
CCAGGAATCGATACAAATTCTGTTTCTTTTATCCAAAATTTATTTCAAGAGTTAGGCGAACTTGGAATCGATAAACAGTT
GTTATTCGATCCTTCTATCATCCGAGGATTTGATTATTACACTGGTTGTATCTTCGAAGTTTTCGATACCAATCCAGAAA
ATAGAAGATCCTTATACGGTGGAGGAAGATACGATAATCTCATCGGTTTGTTTTCCAAGGAACAATTATCCGGAATTGGC
TTTGGATTAGGCGACGTAACACTGAAAAATTTTTTAGAAGGCCATAATCTAATTCCAAATCTAAGTAGAGAAAAAACCAT
TTTTCTTCCAATTATGGATGAATCTCTTTTTGTAGATACTTTCAAACTTTCTAAAGAACTCCGTGAAAATGAAATTCTCA
CCGAAACAATGTTAGATTCCGCAAAAATCGGAAAACAAATTCAAATCGCAGAAAAAAAAGGTTATCGCTACGTATTGTTC
CTTGGAGAATCGGAAATCCGCACGGAAACGGTCCAAATTAAAGACTTAATTTCCGGAGAACAAAAAAGCCTTCCAAGAAA
AGGGCTTTCGGATACTCTTAAAAAAGATTTTCAACTTTGA

Upstream 100 bases:

>100_bases
CTATCACGTAGGCGCAGAAATAGTTGGCTCCAACGATGCTAAGGCAAATATGACTCTATTGAAGAAAAATATGAACTTCA
TTAAGACTCAGTAATTTGCC

Downstream 100 bases:

>100_bases
TAGACATTGTAGATCAAATCGATTTTTCACTCTCTACTTGGATTCGGACAAATCTCCACAATCCTAGATTAAATCGTATT
CTTTCCAAAATCAATCGTGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MENQKNFLTTAPYKGTRDFYPEDMRLRNWMFSVMRETVLSFGYEEYDGPILESFDLYKAKSGEEIVERQLYDFIDKGERR
VAIRPEMTPTLARMVAGNLRNLPKPVRWFSIPNLWRYEQPGKGRLREHWQLNVDLFGVDSHRAELEILLIADSILKKFGA
PIGSYQIKVSHRKLLDSFLKNSLKLNGDQVHGVSKLLDKKSKISSEAFETEMKPFLNNFKEQFSLIETYLNSNLETVSKI
PGIDTNSVSFIQNLFQELGELGIDKQLLFDPSIIRGFDYYTGCIFEVFDTNPENRRSLYGGGRYDNLIGLFSKEQLSGIG
FGLGDVTLKNFLEGHNLIPNLSREKTIFLPIMDESLFVDTFKLSKELRENEILTETMLDSAKIGKQIQIAEKKGYRYVLF
LGESEIRTETVQIKDLISGEQKSLPRKGLSDTLKKDFQL

Sequences:

>Translated_439_residues
MENQKNFLTTAPYKGTRDFYPEDMRLRNWMFSVMRETVLSFGYEEYDGPILESFDLYKAKSGEEIVERQLYDFIDKGERR
VAIRPEMTPTLARMVAGNLRNLPKPVRWFSIPNLWRYEQPGKGRLREHWQLNVDLFGVDSHRAELEILLIADSILKKFGA
PIGSYQIKVSHRKLLDSFLKNSLKLNGDQVHGVSKLLDKKSKISSEAFETEMKPFLNNFKEQFSLIETYLNSNLETVSKI
PGIDTNSVSFIQNLFQELGELGIDKQLLFDPSIIRGFDYYTGCIFEVFDTNPENRRSLYGGGRYDNLIGLFSKEQLSGIG
FGLGDVTLKNFLEGHNLIPNLSREKTIFLPIMDESLFVDTFKLSKELRENEILTETMLDSAKIGKQIQIAEKKGYRYVLF
LGESEIRTETVQIKDLISGEQKSLPRKGLSDTLKKDFQL
>Mature_439_residues
MENQKNFLTTAPYKGTRDFYPEDMRLRNWMFSVMRETVLSFGYEEYDGPILESFDLYKAKSGEEIVERQLYDFIDKGERR
VAIRPEMTPTLARMVAGNLRNLPKPVRWFSIPNLWRYEQPGKGRLREHWQLNVDLFGVDSHRAELEILLIADSILKKFGA
PIGSYQIKVSHRKLLDSFLKNSLKLNGDQVHGVSKLLDKKSKISSEAFETEMKPFLNNFKEQFSLIETYLNSNLETVSKI
PGIDTNSVSFIQNLFQELGELGIDKQLLFDPSIIRGFDYYTGCIFEVFDTNPENRRSLYGGGRYDNLIGLFSKEQLSGIG
FGLGDVTLKNFLEGHNLIPNLSREKTIFLPIMDESLFVDTFKLSKELRENEILTETMLDSAKIGKQIQIAEKKGYRYVLF
LGESEIRTETVQIKDLISGEQKSLPRKGLSDTLKKDFQL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=462, Percent_Identity=26.6233766233766, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI15029520, Length=461, Percent_Identity=25.1626898047722, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1788861, Length=443, Percent_Identity=25.0564334085779, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993686, Length=451, Percent_Identity=28.6031042128603, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71993693, Length=451, Percent_Identity=28.6031042128603, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6325290, Length=462, Percent_Identity=27.9220779220779, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24643061, Length=479, Percent_Identity=25.0521920668058, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24643059, Length=498, Percent_Identity=24.0963855421687, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50620; Mature: 50620

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQKNFLTTAPYKGTRDFYPEDMRLRNWMFSVMRETVLSFGYEEYDGPILESFDLYKAK
CCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCHHHHCC
SGEEIVERQLYDFIDKGERRVAIRPEMTPTLARMVAGNLRNLPKPVRWFSIPNLWRYEQP
CCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCC
GKGRLREHWQLNVDLFGVDSHRAELEILLIADSILKKFGAPIGSYQIKVSHRKLLDSFLK
CCCCCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
NSLKLNGDQVHGVSKLLDKKSKISSEAFETEMKPFLNNFKEQFSLIETYLNSNLETVSKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
PGIDTNSVSFIQNLFQELGELGIDKQLLFDPSIIRGFDYYTGCIFEVFDTNPENRRSLYG
CCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHEEEEECCCCCCCHHCCC
GGRYDNLIGLFSKEQLSGIGFGLGDVTLKNFLEGHNLIPNLSREKTIFLPIMDESLFVDT
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHH
FKLSKELRENEILTETMLDSAKIGKQIQIAEKKGYRYVLFLGESEIRTETVQIKDLISGE
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCH
QKSLPRKGLSDTLKKDFQL
HHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MENQKNFLTTAPYKGTRDFYPEDMRLRNWMFSVMRETVLSFGYEEYDGPILESFDLYKAK
CCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCHHHHCC
SGEEIVERQLYDFIDKGERRVAIRPEMTPTLARMVAGNLRNLPKPVRWFSIPNLWRYEQP
CCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCC
GKGRLREHWQLNVDLFGVDSHRAELEILLIADSILKKFGAPIGSYQIKVSHRKLLDSFLK
CCCCCCCCEEEEEEEEECCCCCCCEEEEEEHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
NSLKLNGDQVHGVSKLLDKKSKISSEAFETEMKPFLNNFKEQFSLIETYLNSNLETVSKI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHC
PGIDTNSVSFIQNLFQELGELGIDKQLLFDPSIIRGFDYYTGCIFEVFDTNPENRRSLYG
CCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHEEEEECCCCCCCHHCCC
GGRYDNLIGLFSKEQLSGIGFGLGDVTLKNFLEGHNLIPNLSREKTIFLPIMDESLFVDT
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHH
FKLSKELRENEILTETMLDSAKIGKQIQIAEKKGYRYVLFLGESEIRTETVQIKDLISGE
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHCCH
QKSLPRKGLSDTLKKDFQL
HHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA