Click here to switch to the map view.

The map label for this gene is pntA

Identifier: 45655960

GI number: 45655960

Start: 56259

End: 57428

Strand: Direct

Name: pntA

Synonym: LIC10046

Alternate gene names: 45655960

Gene position: 56259-57428 (Clockwise)

Preceding gene: 45655959

Following gene: 45655965

Centisome position: 1.32

GC content: 39.91

Gene sequence:

>1170_bases
ATGAACATTGGAATTCTAAAAGAATCTAAAGAAGAAACGAGAGTATCCGTTACACCAGACATTGTAGATGCGCTTAAAAA
AATCGGGGCGACTGTACTCGTAGAAAAAGGCGCTGGAGAACAATCTTATTATCATGATGAAGATTATAAAAAAGCTGGAG
CAAGTCTTGTATCTCGTCAAGATGTTATTAATAAGTCCAATCTCATTGTAAGCATTCATTTTGCCGACCCTACAACATTA
GGAAAAATGAAACCAGGAACCATTTATTTAGGAATGTTCCAACCTGCGATGAATGCACAAACAGTTCAAAAACTTGCTTC
TAAAAAGGTAGAAGTTTTAAGTTTAGATGCAATCGCAAGGATTACGAGAGCCCAGTCTATGGACGTACTTTCTTCTCAGG
CAACGGTTGCCGGTTACAAAGCGGTATTGCTCGCGGCTTCTCATTTAGCCAGATTTTTTCCAATGCTTACTACGGCGGCT
GGAACCATCACACCTGCTTCCGTGTTGATTATTGGAGCCGGAGTTGCTGGATTACAAGCGATCGCTTCCAGTAGAAGATT
GGGCGCGGTAGTAGACGTATTCGATACAAGACCAGAAGTAAAAGAGCAGGTTCATTCTCTTGGAGCGAAGTTTGTAGAAG
TAGAAGGCGCTTCCCATTCTGCTGCTGCTGGCGGTTATGCGGTTGAACAAACCGAAGAATATAAAAAACGTCAACAAGAA
GCGATCGATAAATACGCTCAAAAGGCGGATGTAATCATAACAACTGCGCTCATTCCTGGAAAAAAAGCCCCATTATTGAT
CACTAAAAAGATCGTAGATAATATGAAATCAGGTTCTGTAGTTGTGGATCTTGCTTCTTCTATGGGCGGAAACTGCGAAT
ATACAAAACATGGACAAAATGTAGTTACTCCAAAAGGAGTTACCGTGATCGGTCATAAAAATCTTGCTGGTTCGATTCCT
GCAGACGCATCTAAAATGTTCAGCAAGAACGTTTTAAATTTTCTTAAACTGTTGATTAAGGATAAAAAAATCAATTTAGA
TCTAAATGATGAAATTCTTTCTTCGACTACAATTACTTACCAGGGTGAAGTTCGTCATAAACTTACGTTAGACGCTTTGG
GATCTAAGTCAGGAGCAGCAAAACCTAAGAAGAGCGCTTCCAAGAAATAA

Upstream 100 bases:

>100_bases
GAATACTTTAAAAATAACTTGGATTAAATTTATACAAAGAAATTCTGGACGTACTTTACCGACCAGTCCGGAAGCACTAT
TTGATCACCGGGAATAAATT

Downstream 100 bases:

>100_bases
GCCGAAAAAACCGAAAATCGTCCCGATCATCCAACCTCCGATCACGTCGCTTACAAAGTGATGAAGAGAAAGTAACCTTC
CTACTCCGGCTAAAAAACTG

Product: proton-translocating transhydrogenase subunit alpha part 1

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNIGILKESKEETRVSVTPDIVDALKKIGATVLVEKGAGEQSYYHDEDYKKAGASLVSRQDVINKSNLIVSIHFADPTTL
GKMKPGTIYLGMFQPAMNAQTVQKLASKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVVVDLASSMGGNCEYTKHGQNVVTPKGVTVIGHKNLAGSIP
ADASKMFSKNVLNFLKLLIKDKKINLDLNDEILSSTTITYQGEVRHKLTLDALGSKSGAAKPKKSASKK

Sequences:

>Translated_389_residues
MNIGILKESKEETRVSVTPDIVDALKKIGATVLVEKGAGEQSYYHDEDYKKAGASLVSRQDVINKSNLIVSIHFADPTTL
GKMKPGTIYLGMFQPAMNAQTVQKLASKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVVVDLASSMGGNCEYTKHGQNVVTPKGVTVIGHKNLAGSIP
ADASKMFSKNVLNFLKLLIKDKKINLDLNDEILSSTTITYQGEVRHKLTLDALGSKSGAAKPKKSASKK
>Mature_389_residues
MNIGILKESKEETRVSVTPDIVDALKKIGATVLVEKGAGEQSYYHDEDYKKAGASLVSRQDVINKSNLIVSIHFADPTTL
GKMKPGTIYLGMFQPAMNAQTVQKLASKKVEVLSLDAIARITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAA
GTITPASVLIIGAGVAGLQAIASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVVVDLASSMGGNCEYTKHGQNVVTPKGVTVIGHKNLAGSIP
ADASKMFSKNVLNFLKLLIKDKKINLDLNDEILSSTTITYQGEVRHKLTLDALGSKSGAAKPKKSASKK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=361, Percent_Identity=40.4432132963989, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI122939153, Length=361, Percent_Identity=40.4432132963989, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1787887, Length=367, Percent_Identity=44.6866485013624, Blast_Score=323, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI17550456, Length=355, Percent_Identity=41.1267605633803, Blast_Score=269, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 41419; Mature: 41419

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGILKESKEETRVSVTPDIVDALKKIGATVLVEKGAGEQSYYHDEDYKKAGASLVSRQ
CCCCCCCCCCCCCEEEECHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
DVINKSNLIVSIHFADPTTLGKMKPGTIYLGMFQPAMNAQTVQKLASKKVEVLSLDAIAR
HHCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEHHHHHH
ITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAAGTITPASVLIIGAGVAGLQA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
IASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
HHHCCCCCCEEEHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVVVDLASSMGGNCEYTKHGQN
HHHHHHHHCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCEEEEEHHHCCCCCEEEECCCE
VVTPKGVTVIGHKNLAGSIPADASKMFSKNVLNFLKLLIKDKKINLDLNDEILSSTTITY
EECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCEEEEE
QGEVRHKLTLDALGSKSGAAKPKKSASKK
ECCCEEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIGILKESKEETRVSVTPDIVDALKKIGATVLVEKGAGEQSYYHDEDYKKAGASLVSRQ
CCCCCCCCCCCCCEEEECHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
DVINKSNLIVSIHFADPTTLGKMKPGTIYLGMFQPAMNAQTVQKLASKKVEVLSLDAIAR
HHCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEEHHHHHH
ITRAQSMDVLSSQATVAGYKAVLLAASHLARFFPMLTTAAGTITPASVLIIGAGVAGLQA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHH
IASSRRLGAVVDVFDTRPEVKEQVHSLGAKFVEVEGASHSAAAGGYAVEQTEEYKKRQQE
HHHCCCCCCEEEHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
AIDKYAQKADVIITTALIPGKKAPLLITKKIVDNMKSGSVVVDLASSMGGNCEYTKHGQN
HHHHHHHHCCEEEEEEECCCCCCCEEEEHHHHCCCCCCCEEEEEHHHCCCCCEEEECCCE
VVTPKGVTVIGHKNLAGSIPADASKMFSKNVLNFLKLLIKDKKINLDLNDEILSSTTITY
EECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHCCEEEEE
QGEVRHKLTLDALGSKSGAAKPKKSASKK
ECCCEEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]