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The map label for this gene is gyrB [H]

Identifier: 45655919

GI number: 45655919

Start: 6686

End: 8605

Strand: Direct

Name: gyrB [H]

Synonym: LIC10005

Alternate gene names: 45655919

Gene position: 6686-8605 (Clockwise)

Preceding gene: 45655918

Following gene: 45655920

Centisome position: 0.16

GC content: 37.14

Gene sequence:

>1920_bases
ATGAGCCAAGAAGAAACAAGCTACAGTGCCGGTCAGATTAAAATTTTAGAAGGTCTAGAAGCTGTTAGAAAACGTCCGGG
AATGTATATCGGAACTCAGGACGAAACCGGGCTTCATAAAATGGTTTACGAGGTCGTGGATAACTCGGTTGACGAGGCCA
TGGCTGGTCATTGTACAGAAATTAAAATCAGTATTTTACCGGATAACATCGTTGAGGTGAAAGACAATGGTCGAGGAATT
CCAGTCGATATTCACCCCGACAAAAAAATTTCCACAATCGAAGTTGTTATGACAATCCTTCACGCAGGTGGTAAATTTGA
AAATGATGCCTACAAAGTTTCTGGAGGTTTACATGGGGTTGGGGTTTCGGTAGTGAATGCACTTTCCGAATATCTGGAAG
TTGAAGTTCATCAAAAAGGGAAAGTCTACACTCAAAAATACGAAAAAGGCGTTCCTGTTTCTTCGGTTCAAATTCAAGGA
GATTCTTCGGAAAGGGGAACCATAGTTCGTTTTAAACCGGATGCCACCATTTTTACCACAGTAGATTTTCAATTTGACGT
TCTTTCTGCACGTTTTAGAGAATTAGCTTTTTTGAATAAAGGTTTGGTTTTAGTTGTCGAAGACCATAGACGCGGAAAGG
AAAATATTTTACGAAACGAATTTCAATTTTCCGGTGGAATCGTTTCTTTCGTGGAACATATCAACGAAAACAAACATCCC
ATGCACAAAGTGATTCACTTTGAACGAAATAAAGACGACGTTTTGGCTGAAATTTCGATCCAATATTCGGAAACATATAC
GGAAAACATATTCTGTTTTACAAACAATATAAATAACAATTTAGGTGGAACACATTTAGAAGGTTTTCGTGCGGCTCTTA
CAAGAACATTAAACGACTTTTTAAAAAAGGATTCTACCCTTTCTAAAAAACATCCAACAGGTCTTTCCGGAGAAGATGTA
AAAGAAGGATTAACAGCTGTTATATCGATTAAAATTCCTCAACCTCAATTCAATTCCCAGACAAAAGAAAAATTAGTGAA
CGCGGAAATCAAAGGGATTATGCAAACCTTAAGTTCAGAAGGTTTGACTTTATTTTTTGAAGAGAATCCGAATATTACAA
AAAAGATATTAGAAAAATGTATTCTTTCCGCTAAAGCAAGGGAAGCCGCTCGTAAAGCTAGGGATCTTACTCGAAGAAAA
ACGGTTTTAGAAGGTGGTGGTCTTCCAGGGAAACTTGCGGATTGTTCCGAAAAAGATCCGGCACATTCTGAACTTTATCT
TGTCGAGGGCGATTCTGCGGGTGGTTCCGCAAAACAAGGAAGAGATAGAAATACGCAGGCGATTCTTCCTTTGAAAGGTA
AAATTCTAAACGTTGAAAAAGCAAGACTAGATAAAATCCTTTCCAGTGAAGAAATACGAGTTTTAGTTTCAGCTTTAGGA
ACCGGAATCGGAGAAGATGAATTTAATATAGATAAAATTCGATATCATAAAATTATGATTATGACTGATGCGGATATAGA
CGGTTCTCATATTCGTACTTTGCTTCTTACATTCTTTTTTCGTTATATGCGTCCCGTAATTGAAAGAGGTTATCTTTATG
TTGCACAACCTCCTTTGTATTTAATTAAACACGGCAAAAATTCTACTTACGTTTATTCGGATAAGGAAAAGGAAGAATTA
TTAAAAAACATAGGTGCGGAAAAAGTGGTAATTCAAAGATACAAAGGTTTAGGAGAAATGAATCCGGAGCAACTTTGGGA
AACTACGATGGATCCTTCCAACAGAGTTGTACTAAAAGTTAAGTTAGACGATTTCGTGGAAGCAGAAGAGACATTTAATG
TTCTTATGGGTGACGAAGTTCAACCTCGAAAACAATTCATAGAAGTAAATGCGGCTAAGGTAGCAAACTTAGATCTTTGA

Upstream 100 bases:

>100_bases
AAAAACGTTACCTAGAGATTCTTCAATATCTTTAAGGATTCGATGAATTTCCTTGCCTCCCTATGGTTTTCAGGAAAACT
ATTCCTGAGGTCCGAAAAAT

Downstream 100 bases:

>100_bases
TCGAAAGGGGATTTTGAAATGAGTCAAGAAATGGAAAATGAAACCAAAGTCCTAAGCTATAATATTGCTGGAAAACCAGA
TATTGCAGATGCTCTTAAAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSQEETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIKISILPDNIVEVKDNGRGI
PVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKVYTQKYEKGVPVSSVQIQG
DSSERGTIVRFKPDATIFTTVDFQFDVLSARFRELAFLNKGLVLVVEDHRRGKENILRNEFQFSGGIVSFVEHINENKHP
MHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDSTLSKKHPTGLSGEDV
KEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRK
TVLEGGGLPGKLADCSEKDPAHSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRVLVSALG
TGIGEDEFNIDKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYVYSDKEKEEL
LKNIGAEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNVLMGDEVQPRKQFIEVNAAKVANLDL

Sequences:

>Translated_639_residues
MSQEETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIKISILPDNIVEVKDNGRGI
PVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKVYTQKYEKGVPVSSVQIQG
DSSERGTIVRFKPDATIFTTVDFQFDVLSARFRELAFLNKGLVLVVEDHRRGKENILRNEFQFSGGIVSFVEHINENKHP
MHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDSTLSKKHPTGLSGEDV
KEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRK
TVLEGGGLPGKLADCSEKDPAHSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRVLVSALG
TGIGEDEFNIDKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYVYSDKEKEEL
LKNIGAEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNVLMGDEVQPRKQFIEVNAAKVANLDL
>Mature_638_residues
SQEETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTEIKISILPDNIVEVKDNGRGIP
VDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGVGVSVVNALSEYLEVEVHQKGKVYTQKYEKGVPVSSVQIQGD
SSERGTIVRFKPDATIFTTVDFQFDVLSARFRELAFLNKGLVLVVEDHRRGKENILRNEFQFSGGIVSFVEHINENKHPM
HKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDFLKKDSTLSKKHPTGLSGEDVK
EGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSEGLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRKT
VLEGGGLPGKLADCSEKDPAHSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRVLVSALGT
GIGEDEFNIDKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLYLIKHGKNSTYVYSDKEKEELL
KNIGAEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKVKLDDFVEAEETFNVLMGDEVQPRKQFIEVNAAKVANLDL

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=609, Percent_Identity=28.5714285714286, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI19913406, Length=611, Percent_Identity=25.69558101473, Blast_Score=143, Evalue=6e-34,
Organism=Escherichia coli, GI48994957, Length=556, Percent_Identity=56.8345323741007, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=607, Percent_Identity=39.3739703459638, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17535065, Length=634, Percent_Identity=24.7634069400631, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=532, Percent_Identity=25.9398496240602, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=188, Percent_Identity=31.3829787234043, Blast_Score=80, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=641, Percent_Identity=26.8330733229329, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI17136538, Length=652, Percent_Identity=26.3803680981595, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71576; Mature: 71445

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTE
CCCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEE
IKISILPDNIVEVKDNGRGIPVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGV
EEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEYLEVEVHQKGKVYTQKYEKGVPVSSVQIQGDSSERGTIVRFKPDATIFTT
HHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
VDFQFDVLSARFRELAFLNKGLVLVVEDHRRGKENILRNEFQFSGGIVSFVEHINENKHP
EEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCC
MHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDF
HHHHHEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LKKDSTLSKKHPTGLSGEDVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSE
HHCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRKTVLEGGGLPGKLADCSEKDP
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AHSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRVLVSALG
CCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCHHHHHHHHHHC
TGIGEDEFNIDKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLY
CCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEE
LIKHGKNSTYVYSDKEKEELLKNIGAEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKV
EEEECCCCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
KLDDFVEAEETFNVLMGDEVQPRKQFIEVNAAKVANLDL
ECCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHEECCCC
>Mature Secondary Structure 
SQEETSYSAGQIKILEGLEAVRKRPGMYIGTQDETGLHKMVYEVVDNSVDEAMAGHCTE
CCCCCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEE
IKISILPDNIVEVKDNGRGIPVDIHPDKKISTIEVVMTILHAGGKFENDAYKVSGGLHGV
EEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHH
GVSVVNALSEYLEVEVHQKGKVYTQKYEKGVPVSSVQIQGDSSERGTIVRFKPDATIFTT
HHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCEEEEECCCCEEEEE
VDFQFDVLSARFRELAFLNKGLVLVVEDHRRGKENILRNEFQFSGGIVSFVEHINENKHP
EEEEHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCC
MHKVIHFERNKDDVLAEISIQYSETYTENIFCFTNNINNNLGGTHLEGFRAALTRTLNDF
HHHHHEECCCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
LKKDSTLSKKHPTGLSGEDVKEGLTAVISIKIPQPQFNSQTKEKLVNAEIKGIMQTLSSE
HHCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
GLTLFFEENPNITKKILEKCILSAKAREAARKARDLTRRKTVLEGGGLPGKLADCSEKDP
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AHSELYLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSSEEIRVLVSALG
CCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECCHHHHHHHHHCCHHHHHHHHHHC
TGIGEDEFNIDKIRYHKIMIMTDADIDGSHIRTLLLTFFFRYMRPVIERGYLYVAQPPLY
CCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEE
LIKHGKNSTYVYSDKEKEELLKNIGAEKVVIQRYKGLGEMNPEQLWETTMDPSNRVVLKV
EEEECCCCEEEECCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEE
KLDDFVEAEETFNVLMGDEVQPRKQFIEVNAAKVANLDL
ECCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]