Click here to switch to the map view.

The map label for this gene is recF

Identifier: 45655917

GI number: 45655917

Start: 5062

End: 6159

Strand: Direct

Name: recF

Synonym: LIC10003

Alternate gene names: 45655917

Gene position: 5062-6159 (Clockwise)

Preceding gene: 45655916

Following gene: 45655918

Centisome position: 0.12

GC content: 30.42

Gene sequence:

>1098_bases
ATGTTTTTAAAACATCTTACTATTCAAAACTTTAGAAACCACGAAGAACTGAGTCTAGATTTTGATTCCAGGCTTATTTT
TTTTGTTGGAGATAATGGAGAAGGTAAAACTAATCTTTTAGAAGCGATTTGTATATTATCTTGGTTAAAAAGTTTTCGCG
AATCTGAAGATTCAAACCTTATTCGATGGGGTTCTGAAAATTATTTTCTCAGAGGAAAAATTAAAAATAACCTAAAAGAA
TCCGTTTTAGAAATTGGATTTACTTCTAAACCATCTGTTAAAAGAAAATTAAAATTTAATCAAGAAGAAATAAAAAAAAG
AACAGATCTAATCGGCAAGTTTATCACTGTATTATTAACTCCGATGGATTTAAAAATTATAGAAGGTGGTCCGGCGGAAA
GAAGAAAGTTTATAGACGCTTTTATTTCTTCCTTTGATCCTTTTTATCTTGAATTTTTATTAGAATATAATAAAATTCTA
AAACATAGAAATGCGCTTTTAAAAAGTGGAAATCTAGATATTTCTCATCTTTCTATTTGGGATAAAAAAATCGTAGAAAA
AGGGATCTTTATACTCAACAAAAGAAGAGAAGTTGTTTTGGAATTAAATTCTTTTTATAGGGTTAATTTAGATAAACTGA
GCGGAGGAAAAGACGGACTCGAATTAATCTATAAACCTAATGTAAAAGACCAAGACGAATTTTTAGAAAAACTTAACCGT
AATTTAAGTAGAGATTTAAGGCTTGGTTATACTTCAGTAGGAATTCACAGAGATGATCTTTTTATTGGAACCGATCAAAG
AGATATTACCGAATTTGGTTCCCAAGGTCAAAAAAGAAGTACCGTGATCGCTTTAAAGGCGGCAACTTTTAATTATTACA
AAGATATTTTGAACACCATTCCCGTTTTATTAATTGATGATGTCATTCGAGAGCTGGACGTAAAAAGAAGAGAATACTTT
GTAGATCTTGTAGTTACTGCGGGGCAGGCTTTTTTTACTACGACGGATTTAGAAGGAATTCAAGATTATGTCGGTAAACT
TAAGGATCAAAAGCAAATTTTCCTAATTCGACAAGGAAAAGTCGAGCCTATAAAATGA

Upstream 100 bases:

>100_bases
AGTCAGATTTGCCCGATTTTATTGTGCTAAACTCATTTAATATTAAGATTTTTAAATATTTCTTTTAAAACTTTAAATAG
TCTTTTGGTTATGTATTATA

Downstream 100 bases:

>100_bases
AAGACGATTTAATTTCATCTAAAAAGATCGAAACCTCCGAATTTCGTTCTATTTTAAATCAAATGGGAATTACGGAAGAA
AATCTCCAAGAAAAAATTTC

Product: DNA repair and genetic recombination protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFLKHLTIQNFRNHEELSLDFDSRLIFFVGDNGEGKTNLLEAICILSWLKSFRESEDSNLIRWGSENYFLRGKIKNNLKE
SVLEIGFTSKPSVKRKLKFNQEEIKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLEFLLEYNKIL
KHRNALLKSGNLDISHLSIWDKKIVEKGIFILNKRREVVLELNSFYRVNLDKLSGGKDGLELIYKPNVKDQDEFLEKLNR
NLSRDLRLGYTSVGIHRDDLFIGTDQRDITEFGSQGQKRSTVIALKAATFNYYKDILNTIPVLLIDDVIRELDVKRREYF
VDLVVTAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIRQGKVEPIK

Sequences:

>Translated_365_residues
MFLKHLTIQNFRNHEELSLDFDSRLIFFVGDNGEGKTNLLEAICILSWLKSFRESEDSNLIRWGSENYFLRGKIKNNLKE
SVLEIGFTSKPSVKRKLKFNQEEIKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLEFLLEYNKIL
KHRNALLKSGNLDISHLSIWDKKIVEKGIFILNKRREVVLELNSFYRVNLDKLSGGKDGLELIYKPNVKDQDEFLEKLNR
NLSRDLRLGYTSVGIHRDDLFIGTDQRDITEFGSQGQKRSTVIALKAATFNYYKDILNTIPVLLIDDVIRELDVKRREYF
VDLVVTAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIRQGKVEPIK
>Mature_365_residues
MFLKHLTIQNFRNHEELSLDFDSRLIFFVGDNGEGKTNLLEAICILSWLKSFRESEDSNLIRWGSENYFLRGKIKNNLKE
SVLEIGFTSKPSVKRKLKFNQEEIKKRTDLIGKFITVLLTPMDLKIIEGGPAERRKFIDAFISSFDPFYLEFLLEYNKIL
KHRNALLKSGNLDISHLSIWDKKIVEKGIFILNKRREVVLELNSFYRVNLDKLSGGKDGLELIYKPNVKDQDEFLEKLNR
NLSRDLRLGYTSVGIHRDDLFIGTDQRDITEFGSQGQKRSTVIALKAATFNYYKDILNTIPVLLIDDVIRELDVKRREYF
VDLVVTAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIRQGKVEPIK

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=366, Percent_Identity=28.1420765027322, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_LEPIC (Q72WD4)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_000003.1
- ProteinModelPortal:   Q72WD4
- GeneID:   2772734
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC10003
- NMPDR:   fig|267671.1.peg.3
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK573281
- BioCyc:   LINT267671:LIC_10003-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42430; Mature: 42430

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLKHLTIQNFRNHEELSLDFDSRLIFFVGDNGEGKTNLLEAICILSWLKSFRESEDSNL
CCCCHHHHHHCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IRWGSENYFLRGKIKNNLKESVLEIGFTSKPSVKRKLKFNQEEIKKRTDLIGKFITVLLT
EEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PMDLKIIEGGPAERRKFIDAFISSFDPFYLEFLLEYNKILKHRNALLKSGNLDISHLSIW
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
DKKIVEKGIFILNKRREVVLELNSFYRVNLDKLSGGKDGLELIYKPNVKDQDEFLEKLNR
HHHHHHCCCEEEECCCEEEEEECCEEEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
NLSRDLRLGYTSVGIHRDDLFIGTDQRDITEFGSQGQKRSTVIALKAATFNYYKDILNTI
HHHHHHHCCCEEECEEECCEEEECCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PVLLIDDVIRELDVKRREYFVDLVVTAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIRQGK
HHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCHHHHHHHHHHCCCCCEEEEEECCC
VEPIK
CCCCC
>Mature Secondary Structure
MFLKHLTIQNFRNHEELSLDFDSRLIFFVGDNGEGKTNLLEAICILSWLKSFRESEDSNL
CCCCHHHHHHCCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCE
IRWGSENYFLRGKIKNNLKESVLEIGFTSKPSVKRKLKFNQEEIKKRTDLIGKFITVLLT
EEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PMDLKIIEGGPAERRKFIDAFISSFDPFYLEFLLEYNKILKHRNALLKSGNLDISHLSIW
CCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
DKKIVEKGIFILNKRREVVLELNSFYRVNLDKLSGGKDGLELIYKPNVKDQDEFLEKLNR
HHHHHHCCCEEEECCCEEEEEECCEEEEEHHHCCCCCCCCEEEECCCCCCHHHHHHHHHH
NLSRDLRLGYTSVGIHRDDLFIGTDQRDITEFGSQGQKRSTVIALKAATFNYYKDILNTI
HHHHHHHCCCEEECEEECCEEEECCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PVLLIDDVIRELDVKRREYFVDLVVTAGQAFFTTTDLEGIQDYVGKLKDQKQIFLIRQGK
HHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCHHHHHHHHHHCCCCCEEEEEECCC
VEPIK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA