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The map label for this gene is hisF [H]

Identifier: 45359285

GI number: 45359285

Start: 1660311

End: 1661129

Strand: Direct

Name: hisF [H]

Synonym: MMP1722

Alternate gene names: 45359285

Gene position: 1660311-1661129 (Clockwise)

Preceding gene: 45359284

Following gene: NA

Centisome position: 99.95

GC content: 34.19

Gene sequence:

>819_bases
ATGCTTACTAAAAGAATCATTCCCTGCCTTGATATCAAAGAGGGTAGAGTTGTTAAAGGAACAAATTTTGTAGAGTTAAG
AGATGCCGGAGATCCGGTTGAGCTATCTAAAATTTACAACGAACAGGGTGCTGACGAACTTGTATTTTTGGATATTACCG
CATCTTTTGAAAAAAGAGACATTATTATTGACGTTGTAAAAAGAACTGCCGAACAAGTATTCATTCCATTAACTGTTGGT
GGAGGCATAAAAACAGTTGATGACTTTAGAAAAATATTGAGAGCTGGCGCTGATAAAATTTCAATAAATACATCAGCAGT
AAAAACTCCAGACTTGATAAAAGAAGCTTCTGAAATTTTTGGGACGCAGTGTGTTGTTGTTGCAATGGATGTAAAAAGAA
AGTATATAACAGATTCTCAGGATGAAAATTTGAAAAATAAGAACATCTTTGAAACAAAACTTGGATCCTGCTGGTTTGAA
GTCTATATCTATGGTGGAAGAGAAGGAACTGGTATTGATGCAATAGAATGGGCTAAAAAGGTTGAATATTTAGGTGCTGG
AGAAATTCTTTTAACTAGTATGGATGCAGATGGTACAAAAGATGGGTACGATTTAGTATTGACGAAAGCAATTTCTGAAA
ATACAAAACTTCCAATAATTGCAAGCGGAGGTTGTGGAAATGCAAACCACGTTGTCGATGCATTTACTGAAGGAAAAGCT
GATGCTGCATTAATGGCAAGCATTTTACACTATAGAGAATGTACTGTTAATGATTTGAAAAAAGAAGTTGAAAAAAATAA
TATTCCTGTAAGATTTTAA

Upstream 100 bases:

>100_bases
TTAAAAGTAGCAAAAGAATTAAAAGATATGACATCTAAAAGACAGGATGTTGAAGAAGACGAATTTTAAAAAATCAATTT
ATTACTTTTTTGGTGAATTT

Downstream 100 bases:

>100_bases
TTTTTTAAATTTTTTTGATAGTTTAAAATATCGCTTAATCCAAATATACAAATTAATATGCCCAAAATAAAAAGTGACTA
ACAGCGTCACATTGTTCCCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MLTKRIIPCLDIKEGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDITASFEKRDIIIDVVKRTAEQVFIPLTVG
GGIKTVDDFRKILRAGADKISINTSAVKTPDLIKEASEIFGTQCVVVAMDVKRKYITDSQDENLKNKNIFETKLGSCWFE
VYIYGGREGTGIDAIEWAKKVEYLGAGEILLTSMDADGTKDGYDLVLTKAISENTKLPIIASGGCGNANHVVDAFTEGKA
DAALMASILHYRECTVNDLKKEVEKNNIPVRF

Sequences:

>Translated_272_residues
MLTKRIIPCLDIKEGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDITASFEKRDIIIDVVKRTAEQVFIPLTVG
GGIKTVDDFRKILRAGADKISINTSAVKTPDLIKEASEIFGTQCVVVAMDVKRKYITDSQDENLKNKNIFETKLGSCWFE
VYIYGGREGTGIDAIEWAKKVEYLGAGEILLTSMDADGTKDGYDLVLTKAISENTKLPIIASGGCGNANHVVDAFTEGKA
DAALMASILHYRECTVNDLKKEVEKNNIPVRF
>Mature_272_residues
MLTKRIIPCLDIKEGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDITASFEKRDIIIDVVKRTAEQVFIPLTVG
GGIKTVDDFRKILRAGADKISINTSAVKTPDLIKEASEIFGTQCVVVAMDVKRKYITDSQDENLKNKNIFETKLGSCWFE
VYIYGGREGTGIDAIEWAKKVEYLGAGEILLTSMDADGTKDGYDLVLTKAISENTKLPIIASGGCGNANHVVDAFTEGKA
DAALMASILHYRECTVNDLKKEVEKNNIPVRF

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=276, Percent_Identity=40.5797101449275, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI87082028, Length=230, Percent_Identity=28.2608695652174, Blast_Score=85, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=38.6792452830189, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 29961; Mature: 29961

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDIKEGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDITASFEKRD
CCCCCCCCCEECCCCCEEECCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCH
IIIDVVKRTAEQVFIPLTVGGGIKTVDDFRKILRAGADKISINTSAVKTPDLIKEASEIF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GTQCVVVAMDVKRKYITDSQDENLKNKNIFETKLGSCWFEVYIYGGREGTGIDAIEWAKK
CCEEEEEEEECHHHHCCCCCCCCCCCCCCHHHHHCCEEEEEEEEECCCCCCCHHHHHHHH
VEYLGAGEILLTSMDADGTKDGYDLVLTKAISENTKLPIIASGGCGNANHVVDAFTEGKA
HHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCHHEEHHCCCCC
DAALMASILHYRECTVNDLKKEVEKNNIPVRF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDIKEGRVVKGTNFVELRDAGDPVELSKIYNEQGADELVFLDITASFEKRD
CCCCCCCCCEECCCCCEEECCCEEEECCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCCH
IIIDVVKRTAEQVFIPLTVGGGIKTVDDFRKILRAGADKISINTSAVKTPDLIKEASEIF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
GTQCVVVAMDVKRKYITDSQDENLKNKNIFETKLGSCWFEVYIYGGREGTGIDAIEWAKK
CCEEEEEEEECHHHHCCCCCCCCCCCCCCHHHHHCCEEEEEEEEECCCCCCCHHHHHHHH
VEYLGAGEILLTSMDADGTKDGYDLVLTKAISENTKLPIIASGGCGNANHVVDAFTEGKA
HHHCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCHHEEHHCCCCC
DAALMASILHYRECTVNDLKKEVEKNNIPVRF
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA