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The map label for this gene is cbf5 [H]

Identifier: 45359249

GI number: 45359249

Start: 1627999

End: 1629000

Strand: Direct

Name: cbf5 [H]

Synonym: MMP1686

Alternate gene names: 45359249

Gene position: 1627999-1629000 (Clockwise)

Preceding gene: 45359246

Following gene: 45359250

Centisome position: 98.01

GC content: 36.73

Gene sequence:

>1002_bases
TTGGAATTAATTGTAAAAGAAGAATCAAAAACGGATTATAATTACGGATCTGACCCATACAATAGAGATATAAAAACACT
CTTAAATACTGGGCTCGTTGTAATCGATAAGCCATCTGGCCCTACTTCGCACGAAGTTGCCGCATGGGTTAGAAACATGC
TAAATTTAGTTAAAGCAGGTCACGGCGGAACCCTTGACCCAAAAGTTACTGGAGCACTCCCTGTAGCTTTAGGAAACACG
ACAAAATGCGTTCCAATCTGGCACATCCCACCAAAAGAATATGTGTGTTTGATGCATTTACACGATGATGCTGAGCTTGT
TGATATTGAAAATATTTTTAAAGAATTTACTGGAAGAATTCACCAAAGACCTCCTTTAAAAGCAGCGGTTAAAAGAAGCC
TTAGAATTCGAAAAATATATGAAATTGAGATTTTAGAAATCGATGGGCGAGATATATTATTTAGAACAAAATGCCAATCT
GGAACGTATCTAAGAAAACTCGTAGATGATATGGGTGAAGCGCTTGGAACTTCTGCGCACATGCAGGAACTTAGACGAAC
CATTAGCGGACCATTTTACGAAAATGAAGCTGTTTATTTACAGGATTTACTTGATGCATATATATTCTGGAAGGAAGACG
GAAACGAAGAAGAATTAAGAAAAATAGTAAAACCCCTTGAATACGGGCTACAACATCTTAAAAAAATAATTATAAAGGAT
AGCGCAGTTGATGCAGTGTGCCATGGCGCTACACTTTATTCTTCGGGAATCTCAAAAATTGAAAAGGGGCTTGGAACCGA
TGAAGTTGTTTTGATTGAAACACTGAAAGGAGAGGCTGTTGCAGTTGGAAAACCTCTCATGAATACAAAAGATATGTTAA
AAACTGAAGAAGGCGAAGTTGTAGAAATAACGCGTGTTATAATGGAGCCCGGAATTTATCCAAGAATCTGGAAAAAAAGA
AATAAAAATGATAAAAGTAAACCGGAATTAAAGAAGAAATAA

Upstream 100 bases:

>100_bases
GGAATTATTGTAACTGCATTCAGGCTCTACAAATTTATGAAAAAACACCAAAAACAGTTTATGAAAAATAAAAAACGAAA
ATGAGCCCTAAGGTGAAACT

Downstream 100 bases:

>100_bases
AATTAGCGGTGAATATTATGAAGATAATAATTGATGAAAATTACTGTAAAGGCTGCGACATTTGCATTGAAGTGTGCCCT
AAAGACGTTTACAAAAAGTC

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAGHGGTLDPKVTGALPVALGNT
TKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRIHQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQS
GTYLRKLVDDMGEALGTSAHMQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEVVEITRVIMEPGIYPRIWKKR
NKNDKSKPELKKK

Sequences:

>Translated_333_residues
MELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAGHGGTLDPKVTGALPVALGNT
TKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRIHQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQS
GTYLRKLVDDMGEALGTSAHMQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEVVEITRVIMEPGIYPRIWKKR
NKNDKSKPELKKK
>Mature_333_residues
MELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAGHGGTLDPKVTGALPVALGNT
TKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRIHQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQS
GTYLRKLVDDMGEALGTSAHMQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEVVEITRVIMEPGIYPRIWKKR
NKNDKSKPELKKK

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI4503337, Length=335, Percent_Identity=40.2985074626866, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI215599015, Length=335, Percent_Identity=40.2985074626866, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI21040257, Length=230, Percent_Identity=28.2608695652174, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI2367200, Length=191, Percent_Identity=29.3193717277487, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17553978, Length=320, Percent_Identity=38.75, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323204, Length=321, Percent_Identity=44.2367601246106, Blast_Score=261, Evalue=1e-70,
Organism=Drosophila melanogaster, GI281364189, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI281364187, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI281364185, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI281364183, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62471759, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17975520, Length=320, Percent_Identity=41.25, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR004521 [H]

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 37662; Mature: 37662

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAG
CCEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
HGGTLDPKVTGALPVALGNTTKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRI
CCCCCCCCCCCCEEEEECCCCEEEEEEECCCHHEEEEEEECCCCCEECHHHHHHHHHHHH
HQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQSGTYLRKLVDDMGEALGTSAH
CCCCCHHHHHHHHHHHEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHH
MQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
HHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEV
CHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHCCCCCH
VEITRVIMEPGIYPRIWKKRNKNDKSKPELKKK
HHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAG
CCEEEECCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCC
HGGTLDPKVTGALPVALGNTTKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRI
CCCCCCCCCCCCEEEEECCCCEEEEEEECCCHHEEEEEEECCCCCEECHHHHHHHHHHHH
HQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQSGTYLRKLVDDMGEALGTSAH
CCCCCHHHHHHHHHHHEEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHH
MQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
HHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEV
CHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHCCCCCH
VEITRVIMEPGIYPRIWKKRNKNDKSKPELKKK
HHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA