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The map label for this gene is yqiX [H]

Identifier: 45359195

GI number: 45359195

Start: 1575110

End: 1575832

Strand: Direct

Name: yqiX [H]

Synonym: MMP1632

Alternate gene names: 45359195

Gene position: 1575110-1575832 (Clockwise)

Preceding gene: 45359193

Following gene: 45359199

Centisome position: 94.82

GC content: 33.61

Gene sequence:

>723_bases
ATGAAAAATGCGGTCATATTTTCAGTCCTTTTGGTAATAACCTCGTGCGCATGTATTTCATCTGATTCTAACACGACTAC
ATTGAAAGATGGCGTACTAACTGTAGGGGTGTTTAAATACGTACCACCAGCAGTTTACGAAGAAAATGGAGAATTAAAAG
GTTATGAAGTTGAGTTAATTTCAGAAATTGCAGAACGAATGGATTTAAAAGTGGAATACAAAGAATACATGTTTTCCGAT
CTTTTAAATGCTGTAGCAAATAATGAAGTTGACTGCGCAATATCCGATATTGCAGTAACTCCTGAAAGGGAAAAAATCGT
TTCGTTTAGCAGGGCGTATTCAAAAACTGACATAATTCTTGTGGTACCTAAAGACAGCGACATTGAAAAATTAACAGATA
TCATAGGGCAGAAAGTTGGAGTAACGGCAGGAACTATCGATGATGATAAATCATCTGAACTTTTAAAAAGCATTGATTTT
CAAGTTTGCAGATATAATAACCATGATGAATTATATGATGCATTAGAGAGCGGAAATGTCGATGCAATAATAAGTGAAGA
AAATAACTTTGAATCAGTCCAAAATTCCAGCATAAATTATTTGGATGAACCAATTGCAACGGTTTACTGTGGAATTGCAG
TTAATAAAGATAATAAAGAACTTCGATTAAAAATTAACGATATAATCCTCGAACTTGAACAAGAAGGTTATTTTAACCAG
TGA

Upstream 100 bases:

>100_bases
TATAAAAAATAAAATATTGTCCCCCATATATTTATTTAATGGCTTCTATAATGTTAATTTTAATCTTTTTGTATTTTGAT
AATTTTCTAAGGGGAAACTC

Downstream 100 bases:

>100_bases
TTATTTTAAAACAGGCCAATTATCAGGCATTACTGATCTTTTAATTTTTCTTTTTTGAAACTCTAAAAAAATAAAATTTA
GAGAGTTATTTTAAAATATA

Product: glutamate-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKNAVIFSVLLVITSCACISSDSNTTTLKDGVLTVGVFKYVPPAVYEENGELKGYEVELISEIAERMDLKVEYKEYMFSD
LLNAVANNEVDCAISDIAVTPEREKIVSFSRAYSKTDIILVVPKDSDIEKLTDIIGQKVGVTAGTIDDDKSSELLKSIDF
QVCRYNNHDELYDALESGNVDAIISEENNFESVQNSSINYLDEPIATVYCGIAVNKDNKELRLKINDIILELEQEGYFNQ

Sequences:

>Translated_240_residues
MKNAVIFSVLLVITSCACISSDSNTTTLKDGVLTVGVFKYVPPAVYEENGELKGYEVELISEIAERMDLKVEYKEYMFSD
LLNAVANNEVDCAISDIAVTPEREKIVSFSRAYSKTDIILVVPKDSDIEKLTDIIGQKVGVTAGTIDDDKSSELLKSIDF
QVCRYNNHDELYDALESGNVDAIISEENNFESVQNSSINYLDEPIATVYCGIAVNKDNKELRLKINDIILELEQEGYFNQ
>Mature_240_residues
MKNAVIFSVLLVITSCACISSDSNTTTLKDGVLTVGVFKYVPPAVYEENGELKGYEVELISEIAERMDLKVEYKEYMFSD
LLNAVANNEVDCAISDIAVTPEREKIVSFSRAYSKTDIILVVPKDSDIEKLTDIIGQKVGVTAGTIDDDKSSELLKSIDF
QVCRYNNHDELYDALESGNVDAIISEENNFESVQNSSINYLDEPIATVYCGIAVNKDNKELRLKINDIILELEQEGYFNQ

Specific function: Part of a binding-protein-dependent transport system for arginine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=25.2918287937743, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787031, Length=203, Percent_Identity=27.0935960591133, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788228, Length=212, Percent_Identity=26.4150943396226, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788648, Length=218, Percent_Identity=23.394495412844, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787085, Length=254, Percent_Identity=22.8346456692913, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26753; Mature: 26753

Theoretical pI: Translated: 4.02; Mature: 4.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAVIFSVLLVITSCACISSDSNTTTLKDGVLTVGVFKYVPPAVYEENGELKGYEVELI
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHCCCHHCCCCCCCCCCHHHHH
SEIAERMDLKVEYKEYMFSDLLNAVANNEVDCAISDIAVTPEREKIVSFSRAYSKTDIIL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHCCCCCEEE
VVPKDSDIEKLTDIIGQKVGVTAGTIDDDKSSELLKSIDFQVCRYNNHDELYDALESGNV
EEECCCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHEEEECCHHHHHHHHHCCCE
DAIISEENNFESVQNSSINYLDEPIATVYCGIAVNKDNKELRLKINDIILELEQEGYFNQ
EEEEECCCCHHHHHCCCCCHHHCCHHHEEEEEEECCCCCEEEEEEEHEEEEECCCCCCCC
>Mature Secondary Structure
MKNAVIFSVLLVITSCACISSDSNTTTLKDGVLTVGVFKYVPPAVYEENGELKGYEVELI
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEHHHHHCCCHHCCCCCCCCCCHHHHH
SEIAERMDLKVEYKEYMFSDLLNAVANNEVDCAISDIAVTPEREKIVSFSRAYSKTDIIL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECEEECCCHHHHHHHHHHCCCCCEEE
VVPKDSDIEKLTDIIGQKVGVTAGTIDDDKSSELLKSIDFQVCRYNNHDELYDALESGNV
EEECCCCHHHHHHHHCCCCCCEECCCCCCHHHHHHHHCCHHEEEECCHHHHHHHHHCCCE
DAIISEENNFESVQNSSINYLDEPIATVYCGIAVNKDNKELRLKINDIILELEQEGYFNQ
EEEEECCCCHHHHHCCCCCHHHCCHHHEEEEEEECCCCCEEEEEEEHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]