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The map label for this gene is bioB [H]

Identifier: 45358801

GI number: 45358801

Start: 1223883

End: 1224866

Strand: Direct

Name: bioB [H]

Synonym: MMP1238

Alternate gene names: 45358801

Gene position: 1223883-1224866 (Clockwise)

Preceding gene: 45358800

Following gene: 45358802

Centisome position: 73.68

GC content: 29.57

Gene sequence:

>984_bases
TTGAAGGAAATTAAATTAAATTCTGATTCTTTAGAAATTTACGAAAAATCAGTGTCTGAAAAACTAAATAGAAACGATTT
TATCAAGTTATGGGATTTAGACTTAAATGATTTGCTGGATATTTCATATAATTTAAAAAAATTATTTAACAAAGAAAAAA
TTGATTTGTGCTCAATTATGAATGCTAAATCAGGAATCTGTCCTGAAAACTGCATATTCTGCTCTCAATCAAAACATAAC
ACCTCAAAAATAGATACATATGGATTAAAATCAAAAGAAGAGATTTTAAAAAATGCTAAATCGGTTGAAAAATATTCAAA
CCGCTTTTCAATTGTTGTAAGTGGAAAAACAGTTACAGATTTAGAATTTGAAAAAATTATTGAATCAATTGAAGAAATTC
AAAATAAGACGAAATTAAGGGTTTGCGTTTCATTAGGGCTTTTAAATAAAGATAAATTAAAAGCTTTAAAAGAAAGAAAC
GTAAGAATCCACAACAACCTCGAAACATCTGAAAACTACTTTAAAAATATCTGTACAAGCCACGATTACAGTGAAAAAAT
AAAAGTAATTCTCGAAGCTAAAAAAATAGGGCTTGAAATGTGCAGCGGCGGTATTTTTGGAATGGGTGAAACGATTGAAG
ATAGGGTTGATTTATTTTTAGATTTAAAAAAGCTCGGTGTTGATAGCGTTGCATTAAATCTTTTAAATCCAATTTACGGC
ACCAAAATATACGAAAAAATAAAATCTGGAGATATTTCCCCAATTAACTCCACTGATGCATTGAAATCCATCTGCATTGC
AAGAATTGCCCTTCCAAATAAAGTGATCCGGCTTTGTGGTGGAAGAGAACATGTTTTAAAAGATATGCAGAAATATTCAC
TTCTTGCACTCGATGGTTTAATGATTGGAAACTATTTAACGACAAATGGACAAAATATCCAGTCCGATTTAAAAATGATT
GAAGAAATGGGTTTTGAGCGATGA

Upstream 100 bases:

>100_bases
TGGATCCGAAGAAAACAGATGAATCATTAAATTTAAAATGTAAAAACAAATACTATTTTTAAAATTATTTTAGAAACTTA
TTCACCATAAAGGTGGTTTT

Downstream 100 bases:

>100_bases
TAATTATGGAAAAAGGATTGCTTGAAAAATATAACTCGTTACTTGAGTTTTTCAAAAATAAAAAAGTAATTGTTGCATAT
TCGGGTGGTGTTGACAGCAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKEIKLNSDSLEIYEKSVSEKLNRNDFIKLWDLDLNDLLDISYNLKKLFNKEKIDLCSIMNAKSGICPENCIFCSQSKHN
TSKIDTYGLKSKEEILKNAKSVEKYSNRFSIVVSGKTVTDLEFEKIIESIEEIQNKTKLRVCVSLGLLNKDKLKALKERN
VRIHNNLETSENYFKNICTSHDYSEKIKVILEAKKIGLEMCSGGIFGMGETIEDRVDLFLDLKKLGVDSVALNLLNPIYG
TKIYEKIKSGDISPINSTDALKSICIARIALPNKVIRLCGGREHVLKDMQKYSLLALDGLMIGNYLTTNGQNIQSDLKMI
EEMGFER

Sequences:

>Translated_327_residues
MKEIKLNSDSLEIYEKSVSEKLNRNDFIKLWDLDLNDLLDISYNLKKLFNKEKIDLCSIMNAKSGICPENCIFCSQSKHN
TSKIDTYGLKSKEEILKNAKSVEKYSNRFSIVVSGKTVTDLEFEKIIESIEEIQNKTKLRVCVSLGLLNKDKLKALKERN
VRIHNNLETSENYFKNICTSHDYSEKIKVILEAKKIGLEMCSGGIFGMGETIEDRVDLFLDLKKLGVDSVALNLLNPIYG
TKIYEKIKSGDISPINSTDALKSICIARIALPNKVIRLCGGREHVLKDMQKYSLLALDGLMIGNYLTTNGQNIQSDLKMI
EEMGFER
>Mature_327_residues
MKEIKLNSDSLEIYEKSVSEKLNRNDFIKLWDLDLNDLLDISYNLKKLFNKEKIDLCSIMNAKSGICPENCIFCSQSKHN
TSKIDTYGLKSKEEILKNAKSVEKYSNRFSIVVSGKTVTDLEFEKIIESIEEIQNKTKLRVCVSLGLLNKDKLKALKERN
VRIHNNLETSENYFKNICTSHDYSEKIKVILEAKKIGLEMCSGGIFGMGETIEDRVDLFLDLKKLGVDSVALNLLNPIYG
TKIYEKIKSGDISPINSTDALKSICIARIALPNKVIRLCGGREHVLKDMQKYSLLALDGLMIGNYLTTNGQNIQSDLKMI
EEMGFER

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=301, Percent_Identity=27.2425249169435, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321725, Length=292, Percent_Identity=27.3972602739726, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37126; Mature: 37126

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIKLNSDSLEIYEKSVSEKLNRNDFIKLWDLDLNDLLDISYNLKKLFNKEKIDLCSIM
CCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHH
NAKSGICPENCIFCSQSKHNTSKIDTYGLKSKEEILKNAKSVEKYSNRFSIVVSGKTVTD
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEECH
LEFEKIIESIEEIQNKTKLRVCVSLGLLNKDKLKALKERNVRIHNNLETSENYFKNICTS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCC
HDYSEKIKVILEAKKIGLEMCSGGIFGMGETIEDRVDLFLDLKKLGVDSVALNLLNPIYG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
TKIYEKIKSGDISPINSTDALKSICIARIALPNKVIRLCGGREHVLKDMQKYSLLALDGL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MIGNYLTTNGQNIQSDLKMIEEMGFER
HCCCEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKEIKLNSDSLEIYEKSVSEKLNRNDFIKLWDLDLNDLLDISYNLKKLFNKEKIDLCSIM
CCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHH
NAKSGICPENCIFCSQSKHNTSKIDTYGLKSKEEILKNAKSVEKYSNRFSIVVSGKTVTD
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEECH
LEFEKIIESIEEIQNKTKLRVCVSLGLLNKDKLKALKERNVRIHNNLETSENYFKNICTS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHCC
HDYSEKIKVILEAKKIGLEMCSGGIFGMGETIEDRVDLFLDLKKLGVDSVALNLLNPIYG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
TKIYEKIKSGDISPINSTDALKSICIARIALPNKVIRLCGGREHVLKDMQKYSLLALDGL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
MIGNYLTTNGQNIQSDLKMIEEMGFER
HCCCEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA