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The map label for this gene is leuA [H]

Identifier: 45358626

GI number: 45358626

Start: 1055566

End: 1057110

Strand: Direct

Name: leuA [H]

Synonym: MMP1063

Alternate gene names: 45358626

Gene position: 1055566-1057110 (Clockwise)

Preceding gene: 45358625

Following gene: 161621698

Centisome position: 63.54

GC content: 37.22

Gene sequence:

>1545_bases
ATGGAATCTTATTTAAACAGCAATGAAATTATCAAAAACTCCCTAAAATCAATGAAACTGCCAGAAAAAGTACGTGTTTT
TGACACTACGCTAAGGGATGGAGAACAGACTCCAGGAGTCTCCCTAATGCCTGATCAAAAAATGGATATTGCAAAAAATC
TAAGTGAAATCGGTGTTGATGCAATAGAAGCAGGCTTTCCAGTTTCTTCTGAAGGAGAACAGGAATCTATTAAAAATATA
ACGTCCCTAGGGCTGGATGCAGAAATCTGCGGACTTGCAAGGGCTGTTAAAAAAGATATAGATGTTGCAATTGACTGTGG
AGTTGACAGTATCCACACATTTATTGCTACGTCCCCGCTACATCGGGAATACAAATTAAAAATGAGTAAAGAAAAGATTA
TCGACATTGCAGTTGAATCTATAGAATATATAAAAGAACATGGCGTAATTGTAGAATTTTCCGCAGAAGACGCTACAAGA
ACTGAACTTGATTATTTAAAAGAAGTTTACAAAAAAGCAGTTGAAGCTGGAGCTGACCGGATAAATGTTCCGGATACCGT
TGGTGTTATGGTTCCAAATTCGATGTCCTATTTAATAAAAGAATTAAAAAACGATATAAAAGTTCCAATATCCGTGCACT
GCCACAATGACTTTGGAATTGCTGTATCAAACTCTGTTGCAGCAGTTGAAGCTGGAGCCGAACAGGTACACTGTACTGTA
AACGGCCTTGGTGAAAGAGCGGGAAACGCATCACTAGAAGAAACCGTGATGACTTTAAACATGGTTTATGGAATTAATAC
CAATGTAAATACAAAAATGTTAACAAAACTTTCAAAACTGGTTTCACACTACACTGGAATTAAAACACAGCCTAATAAAG
CAATTGTTGGTGAAAATTCATTTGCACACGAAAGTGGAATTCACGCACACGGTGTTTTAGCAAATGCCCTAACATACGAA
CCAATTGATCCAGAAATAGTTGGAAACAGACGTAGAATTGTTCTTGGAAAACACAGCGGTGCTCACGCAATAAAATCAAA
ACTCTCAGAAATCGGTGTTGAAGTTGGAGAAATCGTTTCAGAAGAGCAGTTCTGTGAAATAGTTGAAAGAGTAAAAGCAA
TTGGCGATAAGGGAAAAATCGTGACAGATGCGGATGTAATGGCAATTGCAGAAGATATTACTAAAAGAACATTGAAATCT
GAAAGAATAGTGGATTTAGAACAGTTTGCAGTAATTACTGGAAATCATGTACTTCCAACTGCAAGCGTTGCGTTGAAAGT
AAAAGATAAAATTTATAAAACGTCTGAACTTGGAGTAGGGCCTGTTGATGCTGCACTAAAAGCAATTCAAGCCGCAGTTG
GTGAAAATATCCGATTAAACGAATACAACATCAGTGCAATTTCTGGAGGAACTGACGCCATTGCAGAAGTTACAGTTAGA
CTTGAAAATCAAGAAAAAGAAGTTATAGCAAAAGCTACTGGCGATGACGTTGTTAAAGCATCCGTAGAAGCAGTTATCGA
TGGAATAAACAAATTAATGGGTTAA

Upstream 100 bases:

>100_bases
ATCGTAAAAATAATTAAATAGTTCTTTACCCTATTATCTTTAAATTCTTTTTAAAATTATATTATTCAAATATTTCTGCT
CCATTGTAATGGTGATATTT

Downstream 100 bases:

>100_bases
TTCCATTTTTTACTTTTTTAAATTATTTAAAAAAATTAAGTTAAATTTAAAGAATTAAATTAATGTAAAAAAAACAGCGG
GATTGTAGAACATATCATTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVDAIEAGFPVSSEGEQESIKNI
TSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPLHREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATR
TELDYLKEVYKKAVEAGADRINVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV
NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENSFAHESGIHAHGVLANALTYE
PIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVSEEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKS
ERIVDLEQFAVITGNHVLPTASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR
LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG

Sequences:

>Translated_514_residues
MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVDAIEAGFPVSSEGEQESIKNI
TSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPLHREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATR
TELDYLKEVYKKAVEAGADRINVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV
NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENSFAHESGIHAHGVLANALTYE
PIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVSEEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKS
ERIVDLEQFAVITGNHVLPTASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR
LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG
>Mature_514_residues
MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVDAIEAGFPVSSEGEQESIKNI
TSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPLHREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATR
TELDYLKEVYKKAVEAGADRINVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV
NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENSFAHESGIHAHGVLANALTYE
PIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVSEEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKS
ERIVDLEQFAVITGNHVLPTASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR
LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=37.7470355731225, Blast_Score=307, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6320071, Length=392, Percent_Identity=32.6530612244898, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320019, Length=358, Percent_Identity=33.2402234636872, Blast_Score=196, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6324682, Length=562, Percent_Identity=23.8434163701068, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=569, Percent_Identity=22.8471001757469, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24582381, Length=306, Percent_Identity=25.1633986928105, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55482; Mature: 55482

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVD
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCHH
AIEAGFPVSSEGEQESIKNITSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPL
HHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCC
HREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATRTELDYLKEVYKKAVEAGADR
CHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
INVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV
CCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHEECCCCCEEEEEE
NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENS
ECHHCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
FAHESGIHAHGVLANALTYEPIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVS
CCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHC
EEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKSERIVDLEQFAVITGNHVLPT
HHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCC
ASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR
CCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHEEEEEEE
LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG
ECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVD
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCHH
AIEAGFPVSSEGEQESIKNITSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPL
HHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCC
HREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATRTELDYLKEVYKKAVEAGADR
CHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCC
INVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV
CCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHEECCCCCEEEEEE
NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENS
ECHHCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
FAHESGIHAHGVLANALTYEPIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVS
CCCCCCCCHHHHHHHCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHC
EEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKSERIVDLEQFAVITGNHVLPT
HHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCC
ASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR
CCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCHHEEEEEEE
LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG
ECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]