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The map label for this gene is cimA [H]

Identifier: 45358581

GI number: 45358581

Start: 1005952

End: 1007430

Strand: Direct

Name: cimA [H]

Synonym: MMP1018

Alternate gene names: 45358581

Gene position: 1005952-1007430 (Clockwise)

Preceding gene: 45358579

Following gene: 45358582

Centisome position: 60.56

GC content: 37.32

Gene sequence:

>1479_bases
ATGGTTAACATATTCGATACTACATTGAGGGATGGAGAACAAACTCCAGGGGTCTCACTATCTCCAAGTGAAAAACTCGA
ACTGGCAATAAAACTTGATGAACTTGGTGTAAATATTATCGAGGCAGGAAGTGCAATAACCTCAAAAGGGGAACGAGAAG
CCATGAAACTGGTTACAGGGCAGAATTTAAATGCTGAAATATCTTCATTTGTAAGGGCCCTTCCAGTAGATATTGATGCT
GCACTTTCCTGTGATGTTGACAGCGTCCATTTAGTCGTTCCAACTTCAGACATCCACATGACCTACAAATTAAGGAAAAG
TCGTGAAGAAAATTTAAAAGATGCAATGCACGCCGTAGAGTATGCTAAAGATCATGGTTTAATCGTCGAACTTTCAGCAG
AAGATGCAACAAGAAGCGATGTTGAATTTTTAAAAGAACTATTCTTAAAAGGGGAAGAACTAAAAGCTGATAGAATCTGT
GTTTGTGATACTGTCGGTATTTTAACGCCCTTAAAAGCAATAGATCTTATAAAAACAATGAAAAATACAATAAAAATTCC
AGTTTCAATCCACTGTCACAACGATTTTGGAATGGCTACCGCAAATACCCTTTCTGCAATAAGTGCGGGTGCAGACCAGT
GCCACGTTACAATTAATGGTATCGGTGAAAGGGCAGGAAATGCGTCACTTGAAGAAGTAGTTATGAGTTTAAAATCACTC
TACGATATAGAAACGGGAATTGATACTGAAAAACTCCATAAAATTTCAAGAATTGTTTCAAAATATATGAAAATTCCAGT
TCCTGCAAATAAAGCGCTTGTTGGTGACAACGCATTTGCTCATGAAGCAGGAATTCACGTTGACGGCCTTATGAAAAGTA
CGGAAACTTATGAGCCAATTCATCCTGAAACAGTTGGAAACAGGAGAAAAATTATACTTGGAAAACACAGCGGAAAAGCT
GCTTTAAAATATAAACTTGAAATAATGAACATTGGATTGAGTCATGAAGAATTTGAAGAAACATATTCAAAAATTAAGGC
TTTTGGAGACCTTGGAAAATACATCAGTGATGTAGACTTAAAAACCATCATAAATCAGGTTAGGGGAGTTGAACTTGAAA
GAAAAGTAATTCTCGATGAAATTACGGTTGTTTCTGGAAACAAAGTATCGCCTCTTGCATCAATAAACTTGAAATTTGCA
AACGACTGCAATATCAAAGACAATGTCCGTGAAGCAGCATACGGTCTTGGTCCAGTTGATGCGGCAATTAATGCAGTTAA
AAAAGCACTCACAGGAGTTGCAGATATTGAACTTGAAGATTACAGTGTTCGTGCAATGACTGGAGGAACTGATGCTTTAA
TTGAAGTAGTAGTTCACTTGAGAAAAGGAAACGAAGTTGTAGAAGTCAAAAAAGCACACGGAGACGTTGTAATGGCGTCT
GTTGAAGCCATGATGGATGGAATCAATTTGCTGATTTAA

Upstream 100 bases:

>100_bases
ATACTTCAAATATTATGGTTTATCAATTAATCAGTGAAATATTATTTAAACTTATCATTCAATTGGCTAAATACTAAAAC
TAATGGTGCGAGAGGATAAA

Downstream 100 bases:

>100_bases
ACTGTTGATTTAAACCATACCCTTACTTTTTTTAAACTTTTAAAACATATATCTAAACGTGATAAACATGTTTAAAAAAT
CTTTATTTATCCTTTTAATA

Product: (R)-citramalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTGQNLNAEISSFVRALPVDIDA
ALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVEYAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRIC
VCDTVGILTPLKAIDLIKTMKNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPIHPETVGNRRKIILGKHSGKA
ALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDLKTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFA
NDCNIKDNVREAAYGLGPVDAAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
VEAMMDGINLLI

Sequences:

>Translated_492_residues
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTGQNLNAEISSFVRALPVDIDA
ALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVEYAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRIC
VCDTVGILTPLKAIDLIKTMKNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPIHPETVGNRRKIILGKHSGKA
ALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDLKTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFA
NDCNIKDNVREAAYGLGPVDAAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
VEAMMDGINLLI
>Mature_492_residues
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTGQNLNAEISSFVRALPVDIDA
ALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVEYAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRIC
VCDTVGILTPLKAIDLIKTMKNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPIHPETVGNRRKIILGKHSGKA
ALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDLKTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFA
NDCNIKDNVREAAYGLGPVDAAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
VEAMMDGINLLI

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=504, Percent_Identity=34.9206349206349, Blast_Score=269, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6320019, Length=360, Percent_Identity=30.5555555555556, Blast_Score=180, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6320071, Length=360, Percent_Identity=30.5555555555556, Blast_Score=179, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6324682, Length=504, Percent_Identity=23.6111111111111, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=23.191094619666, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 53435; Mature: 53435

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTG
CCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCEEEECCCHHHCCCHHHHHHHHCC
QNLNAEISSFVRALPVDIDAALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVE
CCCCHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHH
YAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRICVCDTVGILTPLKAIDLIKTM
HHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
KNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
HHCEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPI
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHCCCCHHHHHCCCCCCCCC
HPETVGNRRKIILGKHSGKAALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDL
CCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
KTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFANDCNIKDNVREAAYGLGPVD
HHHHHHHHCCCHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCHHH
AAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
HHHHHHHHHHCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEHH
VEAMMDGINLLI
HHHHHHHHHHCC
>Mature Secondary Structure
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTG
CCEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCEEEECCCHHHCCCHHHHHHHHCC
QNLNAEISSFVRALPVDIDAALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVE
CCCCHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHH
YAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRICVCDTVGILTPLKAIDLIKTM
HHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
KNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
HHCEEEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHH
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPI
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHCCCCHHHHHCCCCCCCCC
HPETVGNRRKIILGKHSGKAALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDL
CCCCCCCCEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
KTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFANDCNIKDNVREAAYGLGPVD
HHHHHHHHCCCHHHHHHHHHEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCHHH
AAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
HHHHHHHHHHCCCEEEEECCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEHH
VEAMMDGINLLI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 9864346 [H]